Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction
Identifieur interne : 000728 ( Pmc/Curation ); précédent : 000727; suivant : 000729Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction
Auteurs : Mohamed S. Al-Ayed ; Ahmed M. Asaad ; Mohamed A. Qureshi ; Mohammed S. AmeenSource :
- Saudi Medical Journal [ 0379-5284 ] ; 2014.
Abstract
To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.
In a cross-sectional study, 135 children, ≤5 years of age who presented with ARTIs in Najran Maternity and Children Hospital, Najran, Saudi Arabia between October 2012 and July 2013 were included. The clinical and sociodemographic data, and the laboratory results were recorded using a standardized questionnaire. Two nasopharyngeal swabs were collected from each child: one for bacteriological examination, and the second for viral detection using multiplex RT-PCR.
A single viral pathogen was detected in 76 patients, viral coinfections in 9, and mixed viral and bacterial pathogens in 15. Respiratory syncytial virus was isolated in 33 patients, human rhinovirus (hRV) in 22, adenovirus (AdV) in 19, human metapneumovirus in 13, influenza virus in 10, parainfluenza virus in 7, human corona virus (hCoV) in 4, and human bocavirus in one.
Respiratory syncytial virus, hRV, and AdV were the most frequent viruses, accounting for more than two-thirds of the cases. Other viruses, such as MPV, hCoV NL63, and hCoV OC43, may play a role in pediatric ARTIs. Of significance is the potential use of multiplex RT-PCR to provide epidemiological and virological data for early detection of the emergence of novel respiratory viruses in the era of the Middle East respiratory syndrome coronavirus.
Url:
PubMed: 25399211
PubMed Central: 4362149
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000728
Links to Exploration step
PMC:4362149Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction</title><author><name sortKey="Al Ayed, Mohamed S" sort="Al Ayed, Mohamed S" uniqKey="Al Ayed M" first="Mohamed S." last="Al-Ayed">Mohamed S. Al-Ayed</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Asaad, Ahmed M" sort="Asaad, Ahmed M" uniqKey="Asaad A" first="Ahmed M." last="Asaad">Ahmed M. Asaad</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Qureshi, Mohamed A" sort="Qureshi, Mohamed A" uniqKey="Qureshi M" first="Mohamed A." last="Qureshi">Mohamed A. Qureshi</name></author><author><name sortKey="Ameen, Mohammed S" sort="Ameen, Mohammed S" uniqKey="Ameen M" first="Mohammed S." last="Ameen">Mohammed S. Ameen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25399211</idno><idno type="pmc">4362149</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362149</idno><idno type="RBID">PMC:4362149</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000728</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000728</idno><idno type="wicri:Area/Pmc/Curation">000728</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000728</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction</title><author><name sortKey="Al Ayed, Mohamed S" sort="Al Ayed, Mohamed S" uniqKey="Al Ayed M" first="Mohamed S." last="Al-Ayed">Mohamed S. Al-Ayed</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Asaad, Ahmed M" sort="Asaad, Ahmed M" uniqKey="Asaad A" first="Ahmed M." last="Asaad">Ahmed M. Asaad</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Qureshi, Mohamed A" sort="Qureshi, Mohamed A" uniqKey="Qureshi M" first="Mohamed A." last="Qureshi">Mohamed A. Qureshi</name></author><author><name sortKey="Ameen, Mohammed S" sort="Ameen, Mohammed S" uniqKey="Ameen M" first="Mohammed S." last="Ameen">Mohammed S. Ameen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Saudi Medical Journal</title><idno type="ISSN">0379-5284</idno><idno type="eISSN">1658-3175</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Objectives:</title><p>To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.</p></sec><sec id="st2"><title>Methods:</title><p>In a cross-sectional study, 135 children, ≤5 years of age who presented with ARTIs in Najran Maternity and Children Hospital, Najran, Saudi Arabia between October 2012 and July 2013 were included. The clinical and sociodemographic data, and the laboratory results were recorded using a standardized questionnaire. Two nasopharyngeal swabs were collected from each child: one for bacteriological examination, and the second for viral detection using multiplex RT-PCR.</p></sec><sec id="st3"><title>Results:</title><p>A single viral pathogen was detected in 76 patients, viral coinfections in 9, and mixed viral and bacterial pathogens in 15. Respiratory syncytial virus was isolated in 33 patients, human rhinovirus (hRV) in 22, adenovirus (AdV) in 19, human metapneumovirus in 13, influenza virus in 10, parainfluenza virus in 7, human corona virus (hCoV) in 4, and human bocavirus in one.</p></sec><sec id="st4"><title>Conclusion</title><p>Respiratory syncytial virus, hRV, and AdV were the most frequent viruses, accounting for more than two-thirds of the cases. Other viruses, such as MPV, hCoV NL63, and hCoV OC43, may play a role in pediatric ARTIs. Of significance is the potential use of multiplex RT-PCR to provide epidemiological and virological data for early detection of the emergence of novel respiratory viruses in the era of the Middle East respiratory syndrome coronavirus.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bellos, A" uniqKey="Bellos A">A Bellos</name></author><author><name sortKey="Mulholland, K" uniqKey="Mulholland K">K Mulholland</name></author><author><name sortKey="O Rien, Kl" uniqKey="O Rien K">KL O’Brien</name></author><author><name sortKey="Qazi, Sa" uniqKey="Qazi S">SA Qazi</name></author><author><name sortKey="Gayer, M" uniqKey="Gayer M">M Gayer</name></author><author><name sortKey="Checchi, F" uniqKey="Checchi F">F Checchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Der Zalm, Mm" uniqKey="Der Zalm M">MM der Zalm</name></author><author><name sortKey="Van Ewijk, Be" uniqKey="Van Ewijk B">BE van Ewijk</name></author><author><name sortKey="Wilbrink, B" uniqKey="Wilbrink B">B Wilbrink</name></author><author><name sortKey="Uiterwaal, Cs" uniqKey="Uiterwaal C">CS Uiterwaal</name></author><author><name sortKey="Wolfs, Tf" uniqKey="Wolfs T">TF Wolfs</name></author><author><name sortKey="Van Der Ent, Ck" uniqKey="Van Der Ent C">CK van der Ent</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huijskens, Eg" uniqKey="Huijskens E">EG Huijskens</name></author><author><name sortKey="Biesmans, Rc" uniqKey="Biesmans R">RC Biesmans</name></author><author><name sortKey="Buiting, Ag" uniqKey="Buiting A">AG Buiting</name></author><author><name sortKey="Obihara, Cc" uniqKey="Obihara C">CC Obihara</name></author><author><name sortKey="Rossen, Jw" uniqKey="Rossen J">JW Rossen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kwofie, Tb" uniqKey="Kwofie T">TB Kwofie</name></author><author><name sortKey="Anane, Ya" uniqKey="Anane Y">YA Anane</name></author><author><name sortKey="Nkrumah, B" uniqKey="Nkrumah B">B Nkrumah</name></author><author><name sortKey="Annan, A" uniqKey="Annan A">A Annan</name></author><author><name sortKey="Nguah, Sb" uniqKey="Nguah S">SB Nguah</name></author><author><name sortKey="Owusu, M" uniqKey="Owusu M">M Owusu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bicer, S" uniqKey="Bicer S">S Bicer</name></author><author><name sortKey="Giray, T" uniqKey="Giray T">T Giray</name></author><author><name sortKey="Col, D" uniqKey="Col D">D Çöl</name></author><author><name sortKey="Erda, Gc" uniqKey="Erda G">GÇ Erdağ</name></author><author><name sortKey="Vitrinel, A" uniqKey="Vitrinel A">A Vitrinel</name></author><author><name sortKey="Gurol, Y" uniqKey="Gurol Y">Y Gürol</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sloots, Tp" uniqKey="Sloots T">TP Sloots</name></author><author><name sortKey="Whiley, Dm" uniqKey="Whiley D">DM Whiley</name></author><author><name sortKey="Lambert, Sb" uniqKey="Lambert S">SB Lambert</name></author><author><name sortKey="Nissen, Md" uniqKey="Nissen M">MD Nissen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Albuquerque, Mc" uniqKey="Albuquerque M">MC Albuquerque</name></author><author><name sortKey="Pena, Gp" uniqKey="Pena G">GP Pena</name></author><author><name sortKey="Varella, Rb" uniqKey="Varella R">RB Varella</name></author><author><name sortKey="Gallucci, G" uniqKey="Gallucci G">G Gallucci</name></author><author><name sortKey="Erdman, D" uniqKey="Erdman D">D Erdman</name></author><author><name sortKey="Santos, N" uniqKey="Santos N">N Santos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rudan, I" uniqKey="Rudan I">I Rudan</name></author><author><name sortKey="Boschi Pinto, C" uniqKey="Boschi Pinto C">C Boschi-Pinto</name></author><author><name sortKey="Biloglav, Z" uniqKey="Biloglav Z">Z Biloglav</name></author><author><name sortKey="Mulholland, K" uniqKey="Mulholland K">K Mulholland</name></author><author><name sortKey="Campbell, H" uniqKey="Campbell H">H Campbell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fashner, J" uniqKey="Fashner J">J Fashner</name></author><author><name sortKey="Ericson, K" uniqKey="Ericson K">K Ericson</name></author><author><name sortKey="Werner, S" uniqKey="Werner S">S Werner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Advani, S" uniqKey="Advani S">S Advani</name></author><author><name sortKey="Sengupta, A" uniqKey="Sengupta A">A Sengupta</name></author><author><name sortKey="Forman, M" uniqKey="Forman M">M Forman</name></author><author><name sortKey="Valsamakis, A" uniqKey="Valsamakis A">A Valsamakis</name></author><author><name sortKey="Milstone, Am" uniqKey="Milstone A">AM Milstone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bibby, Df" uniqKey="Bibby D">DF Bibby</name></author><author><name sortKey="Mcelarney, I" uniqKey="Mcelarney I">I McElarney</name></author><author><name sortKey="Breuer, J" uniqKey="Breuer J">J Breuer</name></author><author><name sortKey="Clark, Da" uniqKey="Clark D">DA Clark</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Renois, F" uniqKey="Renois F">F Renois</name></author><author><name sortKey="Talmud, D" uniqKey="Talmud D">D Talmud</name></author><author><name sortKey="Huguenin, A" uniqKey="Huguenin A">A Huguenin</name></author><author><name sortKey="Moutte, L" uniqKey="Moutte L">L Moutte</name></author><author><name sortKey="Strady, C" uniqKey="Strady C">C Strady</name></author><author><name sortKey="Cousson, J" uniqKey="Cousson J">J Cousson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Culebras, E" uniqKey="Culebras E">E Culebras</name></author><author><name sortKey="Betriu, C" uniqKey="Betriu C">C Betriu</name></author><author><name sortKey="Vazquez Cid, E" uniqKey="Vazquez Cid E">E Vázquez-Cid</name></author><author><name sortKey="L Pez Varela, E" uniqKey="L Pez Varela E">E López-Varela</name></author><author><name sortKey="Rueda, S" uniqKey="Rueda S">S Rueda</name></author><author><name sortKey="Picazo, Jj" uniqKey="Picazo J">JJ Picazo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Al Hajjar, S" uniqKey="Al Hajjar S">S Al Hajjar</name></author><author><name sortKey="Al Thawadi, S" uniqKey="Al Thawadi S">S Al Thawadi</name></author><author><name sortKey="Al Seraihi, A" uniqKey="Al Seraihi A">A Al Seraihi</name></author><author><name sortKey="Al Muhsen, S" uniqKey="Al Muhsen S">S Al Muhsen</name></author><author><name sortKey="Imambaccus, H" uniqKey="Imambaccus H">H Imambaccus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abdel Moneim, As" uniqKey="Abdel Moneim A">AS Abdel-Moneim</name></author><author><name sortKey="Kamel, Mm" uniqKey="Kamel M">MM Kamel</name></author><author><name sortKey="Al Ghamdi, As" uniqKey="Al Ghamdi A">AS Al-Ghamdi</name></author><author><name sortKey="Al Malky, Mi" uniqKey="Al Malky M">MI Al-Malky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Al Majhdi, Fn" uniqKey="Al Majhdi F">FN Al-Majhdi</name></author><author><name sortKey="Al Jarallah, A" uniqKey="Al Jarallah A">A Al-Jarallah</name></author><author><name sortKey="Elaeed, M" uniqKey="Elaeed M">M Elaeed</name></author><author><name sortKey="Latif, A" uniqKey="Latif A">A Latif</name></author><author><name sortKey="Gissmann, L" uniqKey="Gissmann L">L Gissmann</name></author><author><name sortKey="Amer, Hm" uniqKey="Amer H">HM Amer</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Vandepitte, J" uniqKey="Vandepitte J">J Vandepitte</name></author><author><name sortKey="Verhaegen, J" uniqKey="Verhaegen J">J Verhaegen</name></author><author><name sortKey="Engbaek, K" uniqKey="Engbaek K">K Engbaek</name></author><author><name sortKey="Rohner, P" uniqKey="Rohner P">P Rohner</name></author><author><name sortKey="Piot, P" uniqKey="Piot P">P Piot</name></author><author><name sortKey="Heuck, Cc" uniqKey="Heuck C">CC Heuck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, G" uniqKey="Huang G">G Huang</name></author><author><name sortKey="Yu, D" uniqKey="Yu D">D Yu</name></author><author><name sortKey="Mao, N" uniqKey="Mao N">N Mao</name></author><author><name sortKey="Zhu, Z" uniqKey="Zhu Z">Z Zhu</name></author><author><name sortKey="Zhang, H" uniqKey="Zhang H">H Zhang</name></author><author><name sortKey="Jiang, Z" uniqKey="Jiang Z">Z Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suryadevara, M" uniqKey="Suryadevara M">M Suryadevara</name></author><author><name sortKey="Cummings, E" uniqKey="Cummings E">E Cummings</name></author><author><name sortKey="Bonville, Ca" uniqKey="Bonville C">CA Bonville</name></author><author><name sortKey="Bartholoma, N" uniqKey="Bartholoma N">N Bartholoma</name></author><author><name sortKey="Riddell, S" uniqKey="Riddell S">S Riddell</name></author><author><name sortKey="Kiska, D" uniqKey="Kiska D">D Kiska</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hasman, H" uniqKey="Hasman H">H Hasman</name></author><author><name sortKey="Pachucki, Ct" uniqKey="Pachucki C">CT Pachucki</name></author><author><name sortKey="Unal, A" uniqKey="Unal A">A Unal</name></author><author><name sortKey="Nguyen, D" uniqKey="Nguyen D">D Nguyen</name></author><author><name sortKey="Devlin, T" uniqKey="Devlin T">T Devlin</name></author><author><name sortKey="Peeples, Me" uniqKey="Peeples M">ME Peeples</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martin, Et" uniqKey="Martin E">ET Martin</name></author><author><name sortKey="Kuypers, J" uniqKey="Kuypers J">J Kuypers</name></author><author><name sortKey="Wald, A" uniqKey="Wald A">A Wald</name></author><author><name sortKey="Englund, Ja" uniqKey="Englund J">JA Englund</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, Jk" uniqKey="Kim J">JK Kim</name></author><author><name sortKey="Jeon, Js" uniqKey="Jeon J">JS Jeon</name></author><author><name sortKey="Kim, Jw" uniqKey="Kim J">JW Kim</name></author><author><name sortKey="Rheem, I" uniqKey="Rheem I">I Rheem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Greenberg, Sb" uniqKey="Greenberg S">SB Greenberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jansen, Rr" uniqKey="Jansen R">RR Jansen</name></author><author><name sortKey="Wieringa, J" uniqKey="Wieringa J">J Wieringa</name></author><author><name sortKey="Koekkoek, Sm" uniqKey="Koekkoek S">SM Koekkoek</name></author><author><name sortKey="Visser, Ce" uniqKey="Visser C">CE Visser</name></author><author><name sortKey="Pajkrt, D" uniqKey="Pajkrt D">D Pajkrt</name></author><author><name sortKey="Molenkamp, R" uniqKey="Molenkamp R">R Molenkamp</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Garcia, Ml" uniqKey="Garcia Garcia M">ML García-García</name></author><author><name sortKey="Calvo, C" uniqKey="Calvo C">C Calvo</name></author><author><name sortKey="Falc N, A" uniqKey="Falc N A">A Falcón</name></author><author><name sortKey="Pozo, F" uniqKey="Pozo F">F Pozo</name></author><author><name sortKey="Perez Bre A, P" uniqKey="Perez Bre A P">P Pérez-Breña</name></author><author><name sortKey="De Cea, Jm" uniqKey="De Cea J">JM De Cea</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tregoning, Js" uniqKey="Tregoning J">JS Tregoning</name></author><author><name sortKey="Schwarze, J" uniqKey="Schwarze J">J Schwarze</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Panda, S" uniqKey="Panda S">S Panda</name></author><author><name sortKey="Mohakud, Nk" uniqKey="Mohakud N">NK Mohakud</name></author><author><name sortKey="Pena, L" uniqKey="Pena L">L Pena</name></author><author><name sortKey="Kumar, S" uniqKey="Kumar S">S Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Saudi Med J</journal-id><journal-id journal-id-type="iso-abbrev">Saudi Med J</journal-id><journal-id journal-id-type="publisher-id">SaudiMedJ</journal-id><journal-title-group><journal-title>Saudi Medical Journal</journal-title></journal-title-group><issn pub-type="ppub">0379-5284</issn><issn pub-type="epub">1658-3175</issn><publisher><publisher-name>Saudi Medical Journal</publisher-name><publisher-loc>Saudi Arabia</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25399211</article-id><article-id pub-id-type="pmc">4362149</article-id><article-id pub-id-type="publisher-id">SaudiMedJ-35-1348</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Al-Ayed</surname><given-names>Mohamed S.</given-names></name><degrees>MBBS, MD</degrees><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Asaad</surname><given-names>Ahmed M.</given-names></name><degrees>MBBS, MD</degrees><xref ref-type="aff" rid="aff1"></xref><xref ref-type="corresp" rid="cor1"></xref></contrib><contrib contrib-type="author"><name><surname>Qureshi</surname><given-names>Mohamed A.</given-names></name><degrees>BSc, MSc</degrees></contrib><contrib contrib-type="author"><name><surname>Ameen</surname><given-names>Mohammed S.</given-names></name><degrees>MBBS, MD</degrees><xref ref-type="aff" rid="aff1"></xref></contrib></contrib-group><aff id="aff1"><italic>From the Departments of Pediatrics (Al-Ayed, Ameen), Microbiology (Assad, Qureshi) College of Medicine, Najran University, Najran, Kingdom of Saudi Arabia.</italic></aff><author-notes><corresp id="cor1"><italic>Address correspondence and reprint request to: Dr. Ahmed M. Asaad, Professor of Microbiology, College of Medicine, Najran University, PO Box 1988, Najran, Kingdom of Saudi Arabia. Tel. +966 (17) 5428516. Fax. +966 (17) 5442419. E-mail: <email xlink:href="ahmedmoradasaad@hotmail.com">ahmedmoradasaad@hotmail.com</email>/<email xlink:href="amasad@nu.edu.sa">amasad@nu.edu.sa</email></italic></corresp></author-notes><pub-date pub-type="ppub"><year>2014</year></pub-date><volume>35</volume><issue>11</issue><fpage>1348</fpage><lpage>1353</lpage><history><date date-type="received"><day>29</day><month>5</month><year>2014</year></date><date date-type="accepted"><day>19</day><month>8</month><year>2014</year></date></history><permissions><copyright-statement>Copyright: © Saudi Medical Journal</copyright-statement><copyright-year>2014</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0"><license-p>Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work.</license-p></license></permissions><abstract><sec id="st1"><title>Objectives:</title><p>To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.</p></sec><sec id="st2"><title>Methods:</title><p>In a cross-sectional study, 135 children, ≤5 years of age who presented with ARTIs in Najran Maternity and Children Hospital, Najran, Saudi Arabia between October 2012 and July 2013 were included. The clinical and sociodemographic data, and the laboratory results were recorded using a standardized questionnaire. Two nasopharyngeal swabs were collected from each child: one for bacteriological examination, and the second for viral detection using multiplex RT-PCR.</p></sec><sec id="st3"><title>Results:</title><p>A single viral pathogen was detected in 76 patients, viral coinfections in 9, and mixed viral and bacterial pathogens in 15. Respiratory syncytial virus was isolated in 33 patients, human rhinovirus (hRV) in 22, adenovirus (AdV) in 19, human metapneumovirus in 13, influenza virus in 10, parainfluenza virus in 7, human corona virus (hCoV) in 4, and human bocavirus in one.</p></sec><sec id="st4"><title>Conclusion</title><p>Respiratory syncytial virus, hRV, and AdV were the most frequent viruses, accounting for more than two-thirds of the cases. Other viruses, such as MPV, hCoV NL63, and hCoV OC43, may play a role in pediatric ARTIs. Of significance is the potential use of multiplex RT-PCR to provide epidemiological and virological data for early detection of the emergence of novel respiratory viruses in the era of the Middle East respiratory syndrome coronavirus.</p></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000728 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000728 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:4362149
|texte= Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:25399211" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a CovidV1
| This area was generated with Dilib version V0.6.33. |  |