CD200R1 Supports HSV-1 Viral Replication and Licenses Pro-Inflammatory Signaling Functions of TLR2
Identifieur interne : 000707 ( Pmc/Curation ); précédent : 000706; suivant : 000708CD200R1 Supports HSV-1 Viral Replication and Licenses Pro-Inflammatory Signaling Functions of TLR2
Auteurs : Roy J. Soberman [États-Unis] ; Christopher R. Mackay [États-Unis] ; Christine A. Vaine [États-Unis] ; Glennice Bowen Ryan [États-Unis] ; Anna M. Cerny [États-Unis] ; Mikayla R. Thompson [États-Unis] ; Boris Nikolic [États-Unis] ; Valeria Primo [États-Unis] ; Peter Christmas [États-Unis] ; Paul Sheiffele [États-Unis] ; Lisa Aronov [États-Unis] ; David M. Knipe [États-Unis] ; Evelyn A. Kurt-Jones [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Abstract
The CD200R1:CD200 axis is traditionally considered to limit tissue inflammation by down-regulating pro-inflammatory signaling in myeloid cells bearing the receptor. We generated CD200R1−/− mice and employed them to explore both the role of CD200R1 in regulating macrophage signaling via TLR2 as well as the host response to an
Url:
DOI: 10.1371/journal.pone.0047740
PubMed: 23082204
PubMed Central: 3474780
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000707
Links to Exploration step
PMC:3474780Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">CD200R1 Supports HSV-1 Viral Replication and Licenses Pro-Inflammatory Signaling Functions of TLR2</title><author><name sortKey="Soberman, Roy J" sort="Soberman, Roy J" uniqKey="Soberman R" first="Roy J." last="Soberman">Roy J. Soberman</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Mackay, Christopher R" sort="Mackay, Christopher R" uniqKey="Mackay C" first="Christopher R." last="Mackay">Christopher R. Mackay</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Vaine, Christine A" sort="Vaine, Christine A" uniqKey="Vaine C" first="Christine A." last="Vaine">Christine A. Vaine</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Ryan, Glennice Bowen" sort="Ryan, Glennice Bowen" uniqKey="Ryan G" first="Glennice Bowen" last="Ryan">Glennice Bowen Ryan</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Cerny, Anna M" sort="Cerny, Anna M" uniqKey="Cerny A" first="Anna M." last="Cerny">Anna M. Cerny</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Thompson, Mikayla R" sort="Thompson, Mikayla R" uniqKey="Thompson M" first="Mikayla R." last="Thompson">Mikayla R. Thompson</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Nikolic, Boris" sort="Nikolic, Boris" uniqKey="Nikolic B" first="Boris" last="Nikolic">Boris Nikolic</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Primo, Valeria" sort="Primo, Valeria" uniqKey="Primo V" first="Valeria" last="Primo">Valeria Primo</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Christmas, Peter" sort="Christmas, Peter" uniqKey="Christmas P" first="Peter" last="Christmas">Peter Christmas</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Sheiffele, Paul" sort="Sheiffele, Paul" uniqKey="Sheiffele P" first="Paul" last="Sheiffele">Paul Sheiffele</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>InGenious Targeting Laboratory Incorporated, Stony Brook, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>InGenious Targeting Laboratory Incorporated, Stony Brook, New York</wicri:regionArea></affiliation></author><author><name sortKey="Aronov, Lisa" sort="Aronov, Lisa" uniqKey="Aronov L" first="Lisa" last="Aronov">Lisa Aronov</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>InGenious Targeting Laboratory Incorporated, Stony Brook, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>InGenious Targeting Laboratory Incorporated, Stony Brook, New York</wicri:regionArea></affiliation></author><author><name sortKey="Knipe, David M" sort="Knipe, David M" uniqKey="Knipe D" first="David M." last="Knipe">David M. Knipe</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Kurt Jones, Evelyn A" sort="Kurt Jones, Evelyn A" uniqKey="Kurt Jones E" first="Evelyn A." last="Kurt-Jones">Evelyn A. Kurt-Jones</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23082204</idno><idno type="pmc">3474780</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3474780</idno><idno type="RBID">PMC:3474780</idno><idno type="doi">10.1371/journal.pone.0047740</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000707</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000707</idno><idno type="wicri:Area/Pmc/Curation">000707</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000707</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">CD200R1 Supports HSV-1 Viral Replication and Licenses Pro-Inflammatory Signaling Functions of TLR2</title><author><name sortKey="Soberman, Roy J" sort="Soberman, Roy J" uniqKey="Soberman R" first="Roy J." last="Soberman">Roy J. Soberman</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Mackay, Christopher R" sort="Mackay, Christopher R" uniqKey="Mackay C" first="Christopher R." last="Mackay">Christopher R. Mackay</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Vaine, Christine A" sort="Vaine, Christine A" uniqKey="Vaine C" first="Christine A." last="Vaine">Christine A. Vaine</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Ryan, Glennice Bowen" sort="Ryan, Glennice Bowen" uniqKey="Ryan G" first="Glennice Bowen" last="Ryan">Glennice Bowen Ryan</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Cerny, Anna M" sort="Cerny, Anna M" uniqKey="Cerny A" first="Anna M." last="Cerny">Anna M. Cerny</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Thompson, Mikayla R" sort="Thompson, Mikayla R" uniqKey="Thompson M" first="Mikayla R." last="Thompson">Mikayla R. Thompson</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Nikolic, Boris" sort="Nikolic, Boris" uniqKey="Nikolic B" first="Boris" last="Nikolic">Boris Nikolic</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Primo, Valeria" sort="Primo, Valeria" uniqKey="Primo V" first="Valeria" last="Primo">Valeria Primo</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Christmas, Peter" sort="Christmas, Peter" uniqKey="Christmas P" first="Peter" last="Christmas">Peter Christmas</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Sheiffele, Paul" sort="Sheiffele, Paul" uniqKey="Sheiffele P" first="Paul" last="Sheiffele">Paul Sheiffele</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>InGenious Targeting Laboratory Incorporated, Stony Brook, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>InGenious Targeting Laboratory Incorporated, Stony Brook, New York</wicri:regionArea></affiliation></author><author><name sortKey="Aronov, Lisa" sort="Aronov, Lisa" uniqKey="Aronov L" first="Lisa" last="Aronov">Lisa Aronov</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>InGenious Targeting Laboratory Incorporated, Stony Brook, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>InGenious Targeting Laboratory Incorporated, Stony Brook, New York</wicri:regionArea></affiliation></author><author><name sortKey="Knipe, David M" sort="Knipe, David M" uniqKey="Knipe D" first="David M." last="Knipe">David M. Knipe</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Kurt Jones, Evelyn A" sort="Kurt Jones, Evelyn A" uniqKey="Kurt Jones E" first="Evelyn A." last="Kurt-Jones">Evelyn A. Kurt-Jones</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The CD200R1:CD200 axis is traditionally considered to limit tissue inflammation by down-regulating pro-inflammatory signaling in myeloid cells bearing the receptor. We generated CD200R1<sup>−/−</sup> mice and employed them to explore both the role of CD200R1 in regulating macrophage signaling via TLR2 as well as the host response to an <italic>in vivo</italic>, TLR2-dependent model, herpes simplex virus 1 (HSV-1) infection. CD200R1<sup>−/−</sup> peritoneal macrophages demonstrated a 70–75% decrease in the generation of IL-6 and CCL5 (Rantes) in response to the TLR2 agonist Pam<sub>2</sub>CSK<sub>4</sub> and to HSV-1. CD200R1<sup>−/−</sup> macrophages could neither up-regulate the expression of TLR2, nor assemble a functional inflammasome in response to HSV-1. CD200R1<sup>−/−</sup> mice were protected from HSV-1 infection and exhibited dysfunctional TLR2 signaling. Finally, both CD200R1<sup>−/−</sup> mice and CD200R1<sup>−/−</sup> fibroblasts and macrophages showed a markedly reduced ability to support HSV-1 replication. In summary, our data demonstrate an unanticipated and novel requirement for CD200R1 in “licensing” pro-inflammatory functions of TLR2 and in limiting viral replication that are supported by <italic>ex vivo</italic> and <italic>in vivo</italic> evidence.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Creagh, Em" uniqKey="Creagh E">EM Creagh</name></author><author><name sortKey="O Eill, La" uniqKey="O Eill L">LA O’Neill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dunne, A" uniqKey="Dunne A">A Dunne</name></author><author><name sortKey="O Eill, La" uniqKey="O Eill L">LA O’Neill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Medzhitov, R" uniqKey="Medzhitov R">R Medzhitov</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Kurt Jones, Ea" uniqKey="Kurt Jones E">EA Kurt-Jones</name></author><author><name sortKey="Belko, J" uniqKey="Belko J">J Belko</name></author><author><name sortKey="Yu, C" uniqKey="Yu C">C Yu</name></author><author><name sortKey="Newburger, Pe" uniqKey="Newburger P">PE Newburger</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kurt Jones, Ea" uniqKey="Kurt Jones E">EA Kurt-Jones</name></author><author><name sortKey="Chan, M" uniqKey="Chan M">M Chan</name></author><author><name sortKey="Zhou, S" uniqKey="Zhou S">S Zhou</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author><author><name sortKey="Reed, G" uniqKey="Reed G">G Reed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kurt Jones, Ea" uniqKey="Kurt Jones E">EA Kurt-Jones</name></author><author><name sortKey="Belko, J" uniqKey="Belko J">J Belko</name></author><author><name sortKey="Yu, C" uniqKey="Yu C">C Yu</name></author><author><name sortKey="Newburger, Pe" uniqKey="Newburger P">PE Newburger</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lundberg, P" uniqKey="Lundberg P">P Lundberg</name></author><author><name sortKey="Ramakrishna, C" uniqKey="Ramakrishna C">C Ramakrishna</name></author><author><name sortKey="Brown, J" uniqKey="Brown J">J Brown</name></author><author><name sortKey="Tyszka, Jm" uniqKey="Tyszka J">JM Tyszka</name></author><author><name sortKey="Hamamura, M" uniqKey="Hamamura M">M Hamamura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Jp" uniqKey="Wang J">JP Wang</name></author><author><name sortKey="Bowen, Gn" uniqKey="Bowen G">GN Bowen</name></author><author><name sortKey="Zhou, S" uniqKey="Zhou S">S Zhou</name></author><author><name sortKey="Cerny, A" uniqKey="Cerny A">A Cerny</name></author><author><name sortKey="Zacharia, A" uniqKey="Zacharia A">A Zacharia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Whitley, R" uniqKey="Whitley R">R Whitley</name></author><author><name sortKey="Arvin, A" uniqKey="Arvin A">A Arvin</name></author><author><name sortKey="Prober, C" uniqKey="Prober C">C Prober</name></author><author><name sortKey="Corey, L" uniqKey="Corey L">L Corey</name></author><author><name sortKey="Burchett, S" uniqKey="Burchett S">S Burchett</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Carr, Dj" uniqKey="Carr D">DJ Carr</name></author><author><name sortKey="Campbell, Il" uniqKey="Campbell I">IL Campbell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vilela, Mc" uniqKey="Vilela M">MC Vilela</name></author><author><name sortKey="Mansur, Ds" uniqKey="Mansur D">DS Mansur</name></author><author><name sortKey="Lacerda Queiroz, N" uniqKey="Lacerda Queiroz N">N Lacerda-Queiroz</name></author><author><name sortKey="Rodrigues, Dh" uniqKey="Rodrigues D">DH Rodrigues</name></author><author><name sortKey="Lima, Gk" uniqKey="Lima G">GK Lima</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Melchjorsen, J" uniqKey="Melchjorsen J">J Melchjorsen</name></author><author><name sortKey="Paludan, Sr" uniqKey="Paludan S">SR Paludan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cook, Wj" uniqKey="Cook W">WJ Cook</name></author><author><name sortKey="Kramer, Mf" uniqKey="Kramer M">MF Kramer</name></author><author><name sortKey="Walker, Rm" uniqKey="Walker R">RM Walker</name></author><author><name sortKey="Burwell, Tj" uniqKey="Burwell T">TJ Burwell</name></author><author><name sortKey="Holman, Ha" uniqKey="Holman H">HA Holman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wright, Gj" uniqKey="Wright G">GJ Wright</name></author><author><name sortKey="Puklavec, Mj" uniqKey="Puklavec M">MJ Puklavec</name></author><author><name sortKey="Willis, Ac" uniqKey="Willis A">AC Willis</name></author><author><name sortKey="Hoek, Rm" uniqKey="Hoek R">RM Hoek</name></author><author><name sortKey="Sedgwick, Jd" uniqKey="Sedgwick J">JD Sedgwick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hoek, Rm" uniqKey="Hoek R">RM Hoek</name></author><author><name sortKey="Ruuls, Sr" uniqKey="Ruuls S">SR Ruuls</name></author><author><name sortKey="Murphy, Ca" uniqKey="Murphy C">CA Murphy</name></author><author><name sortKey="Wright, Gj" uniqKey="Wright G">GJ Wright</name></author><author><name sortKey="Goddard, R" uniqKey="Goddard R">R Goddard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Snelgrove, Rj" uniqKey="Snelgrove R">RJ Snelgrove</name></author><author><name sortKey="Goulding, J" uniqKey="Goulding J">J Goulding</name></author><author><name sortKey="Didierlaurent, Am" uniqKey="Didierlaurent A">AM Didierlaurent</name></author><author><name sortKey="Lyonga, D" uniqKey="Lyonga D">D Lyonga</name></author><author><name sortKey="Vekaria, S" uniqKey="Vekaria S">S Vekaria</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, S" uniqKey="Zhang S">S Zhang</name></author><author><name sortKey="Cherwinski, H" uniqKey="Cherwinski H">H Cherwinski</name></author><author><name sortKey="Sedgwick, Jd" uniqKey="Sedgwick J">JD Sedgwick</name></author><author><name sortKey="Phillips, Jh" uniqKey="Phillips J">JH Phillips</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mihrshahi, R" uniqKey="Mihrshahi R">R Mihrshahi</name></author><author><name sortKey="Barclay, An" uniqKey="Barclay A">AN Barclay</name></author><author><name sortKey="Brown, Mh" uniqKey="Brown M">MH Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mihrshahi, R" uniqKey="Mihrshahi R">R Mihrshahi</name></author><author><name sortKey="Brown, Mh" uniqKey="Brown M">MH Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wright, Gj" uniqKey="Wright G">GJ Wright</name></author><author><name sortKey="Cherwinski, H" uniqKey="Cherwinski H">H Cherwinski</name></author><author><name sortKey="Foster Cuevas, M" uniqKey="Foster Cuevas M">M Foster-Cuevas</name></author><author><name sortKey="Brooke, G" uniqKey="Brooke G">G Brooke</name></author><author><name sortKey="Puklavec, Mj" uniqKey="Puklavec M">MJ Puklavec</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mukhopadhyay, S" uniqKey="Mukhopadhyay S">S Mukhopadhyay</name></author><author><name sortKey="Pluddemann, A" uniqKey="Pluddemann A">A Pluddemann</name></author><author><name sortKey="Hoe, Jc" uniqKey="Hoe J">JC Hoe</name></author><author><name sortKey="Williams, Kj" uniqKey="Williams K">KJ Williams</name></author><author><name sortKey="Varin, A" uniqKey="Varin A">A Varin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deckert, M" uniqKey="Deckert M">M Deckert</name></author><author><name sortKey="Sedgwick, Jd" uniqKey="Sedgwick J">JD Sedgwick</name></author><author><name sortKey="Fischer, E" uniqKey="Fischer E">E Fischer</name></author><author><name sortKey="Schluter, D" uniqKey="Schluter D">D Schluter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cortez, M" uniqKey="Cortez M">M Cortez</name></author><author><name sortKey="Huynh, C" uniqKey="Huynh C">C Huynh</name></author><author><name sortKey="Fernandes, Mc" uniqKey="Fernandes M">MC Fernandes</name></author><author><name sortKey="Kennedy, Ka" uniqKey="Kennedy K">KA Kennedy</name></author><author><name sortKey="Aderem, A" uniqKey="Aderem A">A Aderem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Karnam, G" uniqKey="Karnam G">G Karnam</name></author><author><name sortKey="Rygiel, Tp" uniqKey="Rygiel T">TP Rygiel</name></author><author><name sortKey="Raaben, M" uniqKey="Raaben M">M Raaben</name></author><author><name sortKey="Grinwis, Gc" uniqKey="Grinwis G">GC Grinwis</name></author><author><name sortKey="Coenjaerts, Fe" uniqKey="Coenjaerts F">FE Coenjaerts</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shlapatska, Lm" uniqKey="Shlapatska L">LM Shlapatska</name></author><author><name sortKey="Mikhalap, Sv" uniqKey="Mikhalap S">SV Mikhalap</name></author><author><name sortKey="Berdova, Ag" uniqKey="Berdova A">AG Berdova</name></author><author><name sortKey="Zelensky, Om" uniqKey="Zelensky O">OM Zelensky</name></author><author><name sortKey="Yun, Tj" uniqKey="Yun T">TJ Yun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kurt Jones, Ea" uniqKey="Kurt Jones E">EA Kurt-Jones</name></author><author><name sortKey="Sandor, F" uniqKey="Sandor F">F Sandor</name></author><author><name sortKey="Ortiz, Y" uniqKey="Ortiz Y">Y Ortiz</name></author><author><name sortKey="Bowen, Gn" uniqKey="Bowen G">GN Bowen</name></author><author><name sortKey="Counter, Sl" uniqKey="Counter S">SL Counter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="St Leger, Aj" uniqKey="St Leger A">AJ St Leger</name></author><author><name sortKey="Peters, B" uniqKey="Peters B">B Peters</name></author><author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name></author><author><name sortKey="Sette, A" uniqKey="Sette A">A Sette</name></author><author><name sortKey="Hendricks, Rl" uniqKey="Hendricks R">RL Hendricks</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sheridan, Bs" uniqKey="Sheridan B">BS Sheridan</name></author><author><name sortKey="Cherpes, Tl" uniqKey="Cherpes T">TL Cherpes</name></author><author><name sortKey="Urban, J" uniqKey="Urban J">J Urban</name></author><author><name sortKey="Kalinski, P" uniqKey="Kalinski P">P Kalinski</name></author><author><name sortKey="Hendricks, Rl" uniqKey="Hendricks R">RL Hendricks</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taylor, Tj" uniqKey="Taylor T">TJ Taylor</name></author><author><name sortKey="Mcnamee, Ee" uniqKey="Mcnamee E">EE McNamee</name></author><author><name sortKey="Day, C" uniqKey="Day C">C Day</name></author><author><name sortKey="Knipe, Dm" uniqKey="Knipe D">DM Knipe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boudakov, I" uniqKey="Boudakov I">I Boudakov</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="Fan, N" uniqKey="Fan N">N Fan</name></author><author><name sortKey="Gulay, P" uniqKey="Gulay P">P Gulay</name></author><author><name sortKey="Wong, K" uniqKey="Wong K">K Wong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Young, Jl" uniqKey="Young J">JL Young</name></author><author><name sortKey="Mora, A" uniqKey="Mora A">A Mora</name></author><author><name sortKey="Cerny, A" uniqKey="Cerny A">A Cerny</name></author><author><name sortKey="Czech, Mp" uniqKey="Czech M">MP Czech</name></author><author><name sortKey="Woda, B" uniqKey="Woda B">B Woda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mcginnes, Lw" uniqKey="Mcginnes L">LW McGinnes</name></author><author><name sortKey="Gravel, Ka" uniqKey="Gravel K">KA Gravel</name></author><author><name sortKey="Finberg, Rw" uniqKey="Finberg R">RW Finberg</name></author><author><name sortKey="Kurt Jones, Ea" uniqKey="Kurt Jones E">EA Kurt-Jones</name></author><author><name sortKey="Massare, Mj" uniqKey="Massare M">MJ Massare</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mariathasan, S" uniqKey="Mariathasan S">S Mariathasan</name></author><author><name sortKey="Weiss, Ds" uniqKey="Weiss D">DS Weiss</name></author><author><name sortKey="Newton, K" uniqKey="Newton K">K Newton</name></author><author><name sortKey="Mcbride, J" uniqKey="Mcbride J">J McBride</name></author><author><name sortKey="O Ourke, K" uniqKey="O Ourke K">K O’Rourke</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Hornung, V" uniqKey="Hornung V">V Hornung</name></author><author><name sortKey="Ablasser, A" uniqKey="Ablasser A">A Ablasser</name></author><author><name sortKey="Charrel Dennis, M" uniqKey="Charrel Dennis M">M Charrel-Dennis</name></author><author><name sortKey="Bauernfeind, F" uniqKey="Bauernfeind F">F Bauernfeind</name></author><author><name sortKey="Horvath, G" uniqKey="Horvath G">G Horvath</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rathinam, Va" uniqKey="Rathinam V">VA Rathinam</name></author><author><name sortKey="Jiang, Z" uniqKey="Jiang Z">Z Jiang</name></author><author><name sortKey="Waggoner, Sn" uniqKey="Waggoner S">SN Waggoner</name></author><author><name sortKey="Sharma, S" uniqKey="Sharma S">S Sharma</name></author><author><name sortKey="Cole, Le" uniqKey="Cole L">LE Cole</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muruve, Da" uniqKey="Muruve D">DA Muruve</name></author><author><name sortKey="Petrilli, V" uniqKey="Petrilli V">V Petrilli</name></author><author><name sortKey="Zaiss, Ak" uniqKey="Zaiss A">AK Zaiss</name></author><author><name sortKey="White, Lr" uniqKey="White L">LR White</name></author><author><name sortKey="Clark, Sa" uniqKey="Clark S">SA Clark</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vollstedt, S" uniqKey="Vollstedt S">S Vollstedt</name></author><author><name sortKey="Franchini, M" uniqKey="Franchini M">M Franchini</name></author><author><name sortKey="Alber, G" uniqKey="Alber G">G Alber</name></author><author><name sortKey="Ackermann, M" uniqKey="Ackermann M">M Ackermann</name></author><author><name sortKey="Suter, M" uniqKey="Suter M">M Suter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Menachery, Vd" uniqKey="Menachery V">VD Menachery</name></author><author><name sortKey="Pasieka, Tj" uniqKey="Pasieka T">TJ Pasieka</name></author><author><name sortKey="Leib, Da" uniqKey="Leib D">DA Leib</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Detje, Cn" uniqKey="Detje C">CN Detje</name></author><author><name sortKey="Meyer, T" uniqKey="Meyer T">T Meyer</name></author><author><name sortKey="Schmidt, H" uniqKey="Schmidt H">H Schmidt</name></author><author><name sortKey="Kreuz, D" uniqKey="Kreuz D">D Kreuz</name></author><author><name sortKey="Rose, Jk" uniqKey="Rose J">JK Rose</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eidson, Km" uniqKey="Eidson K">KM Eidson</name></author><author><name sortKey="Hobbs, We" uniqKey="Hobbs W">WE Hobbs</name></author><author><name sortKey="Manning, Bj" uniqKey="Manning B">BJ Manning</name></author><author><name sortKey="Carlson, P" uniqKey="Carlson P">P Carlson</name></author><author><name sortKey="Deluca, Na" uniqKey="Deluca N">NA DeLuca</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perry, Ak" uniqKey="Perry A">AK Perry</name></author><author><name sortKey="Chen, G" uniqKey="Chen G">G Chen</name></author><author><name sortKey="Zheng, D" uniqKey="Zheng D">D Zheng</name></author><author><name sortKey="Tang, H" uniqKey="Tang H">H Tang</name></author><author><name sortKey="Cheng, G" uniqKey="Cheng G">G Cheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tabeta, K" uniqKey="Tabeta K">K Tabeta</name></author><author><name sortKey="Georgel, P" uniqKey="Georgel P">P Georgel</name></author><author><name sortKey="Janssen, E" uniqKey="Janssen E">E Janssen</name></author><author><name sortKey="Du, X" uniqKey="Du X">X Du</name></author><author><name sortKey="Hoebe, K" uniqKey="Hoebe K">K Hoebe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hochrein, H" uniqKey="Hochrein H">H Hochrein</name></author><author><name sortKey="Schlatter, B" uniqKey="Schlatter B">B Schlatter</name></author><author><name sortKey="O Eeffe, M" uniqKey="O Eeffe M">M O’Keeffe</name></author><author><name sortKey="Wagner, C" uniqKey="Wagner C">C Wagner</name></author><author><name sortKey="Schmitz, F" uniqKey="Schmitz F">F Schmitz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lund, J" uniqKey="Lund J">J Lund</name></author><author><name sortKey="Sato, A" uniqKey="Sato A">A Sato</name></author><author><name sortKey="Akira, S" uniqKey="Akira S">S Akira</name></author><author><name sortKey="Medzhitov, R" uniqKey="Medzhitov R">R Medzhitov</name></author><author><name sortKey="Iwasaki, A" uniqKey="Iwasaki A">A Iwasaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Davey, Gm" uniqKey="Davey G">GM Davey</name></author><author><name sortKey="Wojtasiak, M" uniqKey="Wojtasiak M">M Wojtasiak</name></author><author><name sortKey="Proietto, Ai" uniqKey="Proietto A">AI Proietto</name></author><author><name sortKey="Carbone, Fr" uniqKey="Carbone F">FR Carbone</name></author><author><name sortKey="Heath, Wr" uniqKey="Heath W">WR Heath</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Koning, N" uniqKey="Koning N">N Koning</name></author><author><name sortKey="Swaab, Df" uniqKey="Swaab D">DF Swaab</name></author><author><name sortKey="Hoek, Rm" uniqKey="Hoek R">RM Hoek</name></author><author><name sortKey="Huitinga, I" uniqKey="Huitinga I">I Huitinga</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Mott, Kr" uniqKey="Mott K">KR Mott</name></author><author><name sortKey="Underhill, D" uniqKey="Underhill D">D Underhill</name></author><author><name sortKey="Wechsler, Sl" uniqKey="Wechsler S">SL Wechsler</name></author><author><name sortKey="Town, T" uniqKey="Town T">T Town</name></author><author><name sortKey="Ghiasi, H" uniqKey="Ghiasi H">H Ghiasi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Lint, Al" uniqKey="Van Lint A">AL van Lint</name></author><author><name sortKey="Murawski, Mr" uniqKey="Murawski M">MR Murawski</name></author><author><name sortKey="Goodbody, Re" uniqKey="Goodbody R">RE Goodbody</name></author><author><name sortKey="Severa, M" uniqKey="Severa M">M Severa</name></author><author><name sortKey="Fitzgerald, Ka" uniqKey="Fitzgerald K">KA Fitzgerald</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Semmling, V" uniqKey="Semmling V">V Semmling</name></author><author><name sortKey="Lukacs Kornek, V" uniqKey="Lukacs Kornek V">V Lukacs-Kornek</name></author><author><name sortKey="Thaiss, Ca" uniqKey="Thaiss C">CA Thaiss</name></author><author><name sortKey="Quast, T" uniqKey="Quast T">T Quast</name></author><author><name sortKey="Hochheiser, K" uniqKey="Hochheiser K">K Hochheiser</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23082204</article-id><article-id pub-id-type="pmc">3474780</article-id><article-id pub-id-type="publisher-id">PONE-D-12-21208</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0047740</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Immunology</subject><subj-group><subject>Immune Response</subject></subj-group></subj-group><subj-group><subject>Microbiology</subject><subj-group><subject>Immunity</subject><subj-group><subject>Inflammation</subject><subject>Innate Immunity</subject></subj-group></subj-group><subj-group><subject>Virology</subject><subj-group><subject>Viral Transmission and Infection</subject><subj-group><subject>Host Cells</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine</subject><subj-group><subject>Infectious Diseases</subject><subj-group><subject>Viral Diseases</subject><subj-group><subject>Herpes Simplex</subject></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>CD200R1 Supports HSV-1 Viral Replication and Licenses Pro-Inflammatory Signaling Functions of TLR2</article-title><alt-title alt-title-type="running-head">CD200R1 Supports HSV Replication and TLR2 Signals</alt-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Soberman</surname><given-names>Roy J.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>MacKay</surname><given-names>Christopher R.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Vaine</surname><given-names>Christine A.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ryan</surname><given-names>Glennice Bowen</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Cerny</surname><given-names>Anna M.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Thompson</surname><given-names>Mikayla R.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nikolic</surname><given-names>Boris</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Primo</surname><given-names>Valeria</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Christmas</surname><given-names>Peter</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sheiffele</surname><given-names>Paul</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Aronov</surname><given-names>Lisa</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Knipe</surname><given-names>David M.</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Kurt-Jones</surname><given-names>Evelyn A.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Renal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America</addr-line></aff><aff id="aff3"><label>3</label><addr-line>InGenious Targeting Laboratory Incorporated, Stony Brook, New York, United States of America</addr-line></aff><aff id="aff4"><label>4</label><addr-line>Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Ansari</surname><given-names>Aftab A.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Emory University School of Medicine, United States of America</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>Soberman@helix.mgh.harvard.edu</email> (RJS); <email>evelyn.kurt-jones@umassmed.edu</email> (EAK-J)</corresp><fn fn-type="COI-statement"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: RJS EAK-J. Performed the experiments: CRM CAV GBR AMC MRT BN VP PC. Analyzed the data: RJS CAV EAK-J. Contributed reagents/materials/analysis tools: PS LA DMK. Wrote the paper: RJS CAV EAK-J.</p></fn></author-notes><pub-date pub-type="collection"><year>2012</year></pub-date><pub-date pub-type="epub"><day>17</day><month>10</month><year>2012</year></pub-date><volume>7</volume><issue>10</issue><elocation-id>e47740</elocation-id><history><date date-type="received"><day>17</day><month>7</month><year>2012</year></date><date date-type="accepted"><day>14</day><month>9</month><year>2012</year></date></history><permissions><copyright-statement>© 2012 Soberman et al</copyright-statement><copyright-year>2012</copyright-year><copyright-holder>Soberman et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.</license-p></license></permissions><abstract><p>The CD200R1:CD200 axis is traditionally considered to limit tissue inflammation by down-regulating pro-inflammatory signaling in myeloid cells bearing the receptor. We generated CD200R1<sup>−/−</sup> mice and employed them to explore both the role of CD200R1 in regulating macrophage signaling via TLR2 as well as the host response to an <italic>in vivo</italic>, TLR2-dependent model, herpes simplex virus 1 (HSV-1) infection. CD200R1<sup>−/−</sup> peritoneal macrophages demonstrated a 70–75% decrease in the generation of IL-6 and CCL5 (Rantes) in response to the TLR2 agonist Pam<sub>2</sub>CSK<sub>4</sub> and to HSV-1. CD200R1<sup>−/−</sup> macrophages could neither up-regulate the expression of TLR2, nor assemble a functional inflammasome in response to HSV-1. CD200R1<sup>−/−</sup> mice were protected from HSV-1 infection and exhibited dysfunctional TLR2 signaling. Finally, both CD200R1<sup>−/−</sup> mice and CD200R1<sup>−/−</sup> fibroblasts and macrophages showed a markedly reduced ability to support HSV-1 replication. In summary, our data demonstrate an unanticipated and novel requirement for CD200R1 in “licensing” pro-inflammatory functions of TLR2 and in limiting viral replication that are supported by <italic>ex vivo</italic> and <italic>in vivo</italic> evidence.</p></abstract><funding-group><funding-statement>This work was supported by grants R01AI068871 (RJS), R01AI068871-S4 (RJS and CV), P01 AI083215-01 (EAK-J and DMK) and UMass CFAR Grant 5P30 AI-42845 (EAK-J) from the National Institutes of Health. Core resources supported by the Diabetes Endocrinology Research Center grant DK32520 were also used. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="12"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000707 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000707 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:3474780
|texte= CD200R1 Supports HSV-1 Viral Replication and Licenses Pro-Inflammatory Signaling Functions of TLR2
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:23082204" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a CovidV1
| This area was generated with Dilib version V0.6.33. |  |