Comparison of electron microscopy, enzyme-linked immunosorbent assay, solid-phase radioimmunoassay, and indirect immunofluorescence for detection of human rotavirus antigen in faeces.
Identifieur interne : 000235 ( Pmc/Curation ); précédent : 000234; suivant : 000236Comparison of electron microscopy, enzyme-linked immunosorbent assay, solid-phase radioimmunoassay, and indirect immunofluorescence for detection of human rotavirus antigen in faeces.
Auteurs : C J Birch ; N I Lehmann ; A J Hawker ; J A Marshall ; I D GustSource :
- Journal of Clinical Pathology [ 0021-9746 ] ; 1979.
Abstract
Four techniques were compared for their practicability, speed, and sensitivity for the detection of human rotavirus. Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) were found to be the most sensitive means of identifying rotavirus, and, once processed, up to 40 specimens could be examined daily. Electron microscopy, although less sensitive than these techniques, had the advantage of being able to detect other viral agents present in faecal extracts. Indirect immunofluorescence failed to detect rotavirus as often as the other three methods. In laboratories where routine examination of faecal specimens from patients with gastroenteritis is required, ELISA and RIA are useful alternatives to electron microscopy.
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PubMed: 227941
PubMed Central: 1145778
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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Pathol</journal-id><journal-title>Journal of Clinical Pathology</journal-title><issn pub-type="ppub">0021-9746</issn><issn pub-type="epub">1472-4146</issn></journal-meta><article-meta><article-id pub-id-type="pmid">227941</article-id><article-id pub-id-type="pmc">1145778</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Comparison of electron microscopy, enzyme-linked immunosorbent assay, solid-phase radioimmunoassay, and indirect immunofluorescence for detection of human rotavirus antigen in faeces.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Birch</surname><given-names>C J</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lehmann</surname><given-names>N I</given-names></name></contrib><contrib contrib-type="author"><name><surname>Hawker</surname><given-names>A J</given-names></name></contrib><contrib contrib-type="author"><name><surname>Marshall</surname><given-names>J A</given-names></name></contrib><contrib contrib-type="author"><name><surname>Gust</surname><given-names>I D</given-names></name></contrib></contrib-group><pub-date pub-type="ppub"><month>7</month><year>1979</year></pub-date><volume>32</volume><issue>7</issue><fpage>700</fpage><lpage>705</lpage><abstract><p>Four techniques were compared for their practicability, speed, and sensitivity for the detection of human rotavirus. Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) were found to be the most sensitive means of identifying rotavirus, and, once processed, up to 40 specimens could be examined daily. Electron microscopy, although less sensitive than these techniques, had the advantage of being able to detect other viral agents present in faecal extracts. Indirect immunofluorescence failed to detect rotavirus as often as the other three methods. In laboratories where routine examination of faecal specimens from patients with gastroenteritis is required, ELISA and RIA are useful alternatives to electron microscopy.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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