Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells
Identifieur interne : 000246 ( Pmc/Corpus ); précédent : 000245; suivant : 000247Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells
Auteurs : Evelyn Albu ; Kathryn V. HolmesSource :
- Journal of Virology [ 0022-538X ] ; 1973.
Abstract
An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70
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PubMed: 4797266
PubMed Central: 356749
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<author><name sortKey="Albu, Evelyn" sort="Albu, Evelyn" uniqKey="Albu E" first="Evelyn" last="Albu">Evelyn Albu</name>
</author>
<author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V." last="Holmes">Kathryn V. Holmes</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells</title>
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<author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V." last="Holmes">Kathryn V. Holmes</name>
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<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
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<imprint><date when="1973">1973</date>
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<front><div type="abstract" xml:lang="en"><p>An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70<italic>S</italic>
in sucrose density gradients and has a molecular weight of approximately 10<sup>7</sup>
, as estimated by gel electrophoresis. The R-type VLP can be labeled with <sup>3</sup>
H-uridine in the presence of actinomycin D. On the basis of morphology, site of maturation, and preliminary biochemical characterization, the R-type VLP does not appear to fit into any of the major groups of animal viruses.</p>
<sec sec-type="scanned-figures"><title>Images</title>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id>
<journal-title>Journal of Virology</journal-title>
<issn pub-type="ppub">0022-538X</issn>
<issn pub-type="epub">1098-5514</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">4797266</article-id>
<article-id pub-id-type="pmc">356749</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Animal Viruses</subject>
</subj-group>
</article-categories>
<title-group><article-title>Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells</article-title>
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<contrib-group><contrib contrib-type="author"><name><surname>Albu</surname>
<given-names>Evelyn</given-names>
</name>
<xref ref-type="author-notes" rid="au1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Holmes</surname>
<given-names>Kathryn V.</given-names>
</name>
<xref ref-type="author-notes" rid="au2">2</xref>
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<aff id="af1"><label>a</label>
Department of Microbiology, Georgetown University Schools of Medicine and Dentistry, Washington, D.C. 20007</aff>
<author-notes><fn id="au1"><label>1</label>
<p> Present address: Schering Corporation, Galloping Hill Rd., Kenilworth, N.J. 07033.</p>
</fn>
<fn id="au2"><label>2</label>
<p> Present address: Department of Microbiology, The University of Texas Southwestern Medical School, Dallas, Tex. 75235.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>11</month>
<year>1973</year>
</pub-date>
<volume>12</volume>
<issue>5</issue>
<fpage>1164</fpage>
<lpage>1172</lpage>
<copyright-statement>Copyright © 1973 American Society for Microbiology</copyright-statement>
<abstract><p>An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70<italic>S</italic>
in sucrose density gradients and has a molecular weight of approximately 10<sup>7</sup>
, as estimated by gel electrophoresis. The R-type VLP can be labeled with <sup>3</sup>
H-uridine in the presence of actinomycin D. On the basis of morphology, site of maturation, and preliminary biochemical characterization, the R-type VLP does not appear to fit into any of the major groups of animal viruses.</p>
<sec sec-type="scanned-figures"><title>Images</title>
<fig id="F1"><graphic xlink:href="jvirol00263-0230-a" xlink:role="1166"></graphic>
</fig>
<fig id="F2"><graphic xlink:href="jvirol00263-0230-b" xlink:role="1166"></graphic>
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</fig>
</sec>
</abstract>
</article-meta>
</front>
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