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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of a multiplex RT-PCR for simultaneous diagnosis of human metapneumovirus (HMPV) and human respiratory syncytial virus (HRSV) from clinical specimens</title><author><name sortKey="Dayakar, Seetha" sort="Dayakar, Seetha" uniqKey="Dayakar S" first="Seetha" last="Dayakar">Seetha Dayakar</name><affiliation><nlm:aff id="Aff1">Laboratory Medicine and Molecular Diagnostics, Rajiv Gandhi Centre for Biotechnology, Bio-Innovation Center (BIC), KINFRA Film & Video Park, Thiruvananthapuram, Kerala 695585 India</nlm:aff></affiliation></author><author><name sortKey="Pillai, Heera R" sort="Pillai, Heera R" uniqKey="Pillai H" first="Heera R." last="Pillai">Heera R. Pillai</name><affiliation><nlm:aff id="Aff1">Laboratory Medicine and Molecular Diagnostics, Rajiv Gandhi Centre for Biotechnology, Bio-Innovation Center (BIC), KINFRA Film & Video Park, Thiruvananthapuram, Kerala 695585 India</nlm:aff></affiliation></author><author><name sortKey="Thulasi, Vineetha P" sort="Thulasi, Vineetha P" uniqKey="Thulasi V" first="Vineetha P." last="Thulasi">Vineetha P. Thulasi</name><affiliation><nlm:aff id="Aff1">Laboratory Medicine and Molecular Diagnostics, Rajiv Gandhi Centre for Biotechnology, Bio-Innovation Center (BIC), KINFRA Film & Video Park, Thiruvananthapuram, Kerala 695585 India</nlm:aff></affiliation></author><author><name sortKey="Nair, Radhakrishnan R" sort="Nair, Radhakrishnan R" uniqKey="Nair R" first="Radhakrishnan R." last="Nair">Radhakrishnan R. Nair</name><affiliation><nlm:aff id="Aff1">Laboratory Medicine and Molecular Diagnostics, Rajiv Gandhi Centre for Biotechnology, Bio-Innovation Center (BIC), KINFRA Film & Video Park, Thiruvananthapuram, Kerala 695585 India</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28004017</idno><idno type="pmc">5142592</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5142592</idno><idno type="RBID">PMC:5142592</idno><idno type="doi">10.1007/s13337-016-0348-2</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000096</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000096</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development of a multiplex RT-PCR for simultaneous diagnosis of human metapneumovirus (HMPV) and human respiratory syncytial virus (HRSV) from clinical specimens</title><author><name sortKey="Dayakar, Seetha" sort="Dayakar, Seetha" uniqKey="Dayakar S" first="Seetha" last="Dayakar">Seetha Dayakar</name><affiliation><nlm:aff id="Aff1">Laboratory Medicine and Molecular Diagnostics, Rajiv Gandhi Centre for Biotechnology, Bio-Innovation Center (BIC), KINFRA Film & Video Park, Thiruvananthapuram, Kerala 695585 India</nlm:aff></affiliation></author><author><name sortKey="Pillai, Heera R" sort="Pillai, Heera R" uniqKey="Pillai H" first="Heera R." last="Pillai">Heera R. Pillai</name><affiliation><nlm:aff id="Aff1">Laboratory Medicine and Molecular Diagnostics, Rajiv Gandhi Centre for Biotechnology, Bio-Innovation Center (BIC), KINFRA Film & Video Park, Thiruvananthapuram, Kerala 695585 India</nlm:aff></affiliation></author><author><name sortKey="Thulasi, Vineetha P" sort="Thulasi, Vineetha P" uniqKey="Thulasi V" first="Vineetha P." last="Thulasi">Vineetha P. Thulasi</name><affiliation><nlm:aff id="Aff1">Laboratory Medicine and Molecular Diagnostics, Rajiv Gandhi Centre for Biotechnology, Bio-Innovation Center (BIC), KINFRA Film & Video Park, Thiruvananthapuram, Kerala 695585 India</nlm:aff></affiliation></author><author><name sortKey="Nair, Radhakrishnan R" sort="Nair, Radhakrishnan R" uniqKey="Nair R" first="Radhakrishnan R." last="Nair">Radhakrishnan R. Nair</name><affiliation><nlm:aff id="Aff1">Laboratory Medicine and Molecular Diagnostics, Rajiv Gandhi Centre for Biotechnology, Bio-Innovation Center (BIC), KINFRA Film & Video Park, Thiruvananthapuram, Kerala 695585 India</nlm:aff></affiliation></author></analytic><series><title level="j">VirusDisease</title><idno type="ISSN">2347-3584</idno><idno type="eISSN">2347-3517</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Human metapneumovirus (HMPV) and human respiratory syncytial virus (HRSV) are ubiquitous respiratory viral pathogens. They belong to the family <italic>Paramyxoviridae</italic> (subfamily <italic>Pneumovirinae</italic>) and is responsible for acute respiratory tract infections in children, elderly and immunocompromised patients. We designed and tested a multiplex reverse transcriptase polymerase chain reaction (mRT-PCR) as a cost-effective alternative to real-time PCR and cell culture based detection for HMPV and HRSV. The newly developed PCR was used to screen nasal/throat swab samples from 356 patients with suspected acute respiratory infection attending the Government Medical College, Thiruvananthapuram, Kerala, India. The method was compared with a commercially available kit employing real time PCR, for its sensitivity and specificity. 53 (14.9 %) samples were positive for at least one tested pathogen by mRT-PCR. All except one among the positive samples showed similar pathogen profile when tested using real time PCR. 8 (15.1 %) among these 53 were positive for HRSVA, 33 (62.3 %) positive for HRSVB and 12 (22.6 %) were positive for HMPV. 17 (32.7 %) samples showed co-infections in them. Sensitivity and specificity of the mRT-PCR was comparable to that of the commercial kit. Our findings indicate that this newly developed mRT-PCR can be used as a cost-effective alternative for laboratory diagnosis of HMPV/HRSV infection and will significantly reduce diagnostic costs for these viruses in clinical settings.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s13337-016-0348-2) contains supplementary material, which is available to authorized users.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virusdisease</journal-id><journal-id journal-id-type="iso-abbrev">Virusdisease</journal-id><journal-title-group><journal-title>VirusDisease</journal-title></journal-title-group><issn pub-type="ppub">2347-3584</issn><issn pub-type="epub">2347-3517</issn><publisher><publisher-name>Springer India</publisher-name><publisher-loc>New Delhi</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28004017</article-id><article-id pub-id-type="pmc">5142592</article-id><article-id pub-id-type="publisher-id">348</article-id><article-id pub-id-type="doi">10.1007/s13337-016-0348-2</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Development of a multiplex RT-PCR for simultaneous diagnosis of human metapneumovirus (HMPV) and human respiratory syncytial virus (HRSV) from clinical specimens</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dayakar</surname><given-names>Seetha</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Pillai</surname><given-names>Heera R.</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Thulasi</surname><given-names>Vineetha P.</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Nair</surname><given-names>Radhakrishnan R.</given-names></name><address><phone>+91 471 2781222</phone><email>radhakrishnan@rgcb.res.in</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1">Laboratory Medicine and Molecular Diagnostics, Rajiv Gandhi Centre for Biotechnology, Bio-Innovation Center (BIC), KINFRA Film & Video Park, Thiruvananthapuram, Kerala 695585 India</aff></contrib-group><pub-date pub-type="epub"><day>26</day><month>9</month><year>2016</year></pub-date><pub-date pub-type="ppub"><month>12</month><year>2016</year></pub-date><volume>27</volume><issue>4</issue><fpage>375</fpage><lpage>381</lpage><history><date date-type="received"><day>29</day><month>3</month><year>2016</year></date><date date-type="accepted"><day>12</day><month>9</month><year>2016</year></date></history><permissions><copyright-statement>© Indian Virological Society 2016</copyright-statement></permissions><abstract id="Abs1"><p>Human metapneumovirus (HMPV) and human respiratory syncytial virus (HRSV) are ubiquitous respiratory viral pathogens. They belong to the family <italic>Paramyxoviridae</italic> (subfamily <italic>Pneumovirinae</italic>) and is responsible for acute respiratory tract infections in children, elderly and immunocompromised patients. We designed and tested a multiplex reverse transcriptase polymerase chain reaction (mRT-PCR) as a cost-effective alternative to real-time PCR and cell culture based detection for HMPV and HRSV. The newly developed PCR was used to screen nasal/throat swab samples from 356 patients with suspected acute respiratory infection attending the Government Medical College, Thiruvananthapuram, Kerala, India. The method was compared with a commercially available kit employing real time PCR, for its sensitivity and specificity. 53 (14.9 %) samples were positive for at least one tested pathogen by mRT-PCR. All except one among the positive samples showed similar pathogen profile when tested using real time PCR. 8 (15.1 %) among these 53 were positive for HRSVA, 33 (62.3 %) positive for HRSVB and 12 (22.6 %) were positive for HMPV. 17 (32.7 %) samples showed co-infections in them. Sensitivity and specificity of the mRT-PCR was comparable to that of the commercial kit. Our findings indicate that this newly developed mRT-PCR can be used as a cost-effective alternative for laboratory diagnosis of HMPV/HRSV infection and will significantly reduce diagnostic costs for these viruses in clinical settings.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s13337-016-0348-2) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Acute respiratory infection</kwd><kwd>Human metapneumovirus</kwd><kwd>Human respiratory syncytial virus</kwd><kwd>Multiplex reverse transcriptase PCR</kwd><kwd>Real-time reverse transcriptase PCR</kwd></kwd-group><funding-group><award-group><funding-source><institution>intramural research RGCB</institution></funding-source></award-group></funding-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Indian Virological Society 2016</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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