The Nucleocapsid Protein of Human Coronavirus NL63
Identifieur interne : 000308 ( Pmc/Checkpoint ); précédent : 000307; suivant : 000309The Nucleocapsid Protein of Human Coronavirus NL63
Auteurs : Kaja Zuwała [Pologne] ; Anna Golda [Pologne] ; Wojciech Kabala [Pologne] ; Michał Burmistrz [Pologne] ; Michal Zdzalik [Pologne] ; Paulina Nowak [Pologne] ; Sylwia Kedracka-Krok [Pologne] ; Mirosław Zarebski [Pologne] ; Jerzy Dobrucki [Pologne] ; Dominik Florek [Pologne] ; Sławomir Zeglen [Pologne] ; Jacek Wojarski [Pologne] ; Jan Potempa [Pologne, États-Unis] ; Grzegorz Dubin [Pologne] ; Krzysztof Pyrc [Pologne]Source :
- PLoS ONE [ 1932-6203 ] ; 2015.
Abstract
Human coronavirus (HCoV) NL63 was first described in 2004 and is associated with respiratory tract disease of varying severity. At the genetic and structural level, HCoV-NL63 is similar to other members of the
Url:
DOI: 10.1371/journal.pone.0117833
PubMed: 25700263
PubMed Central: 4336326
Affiliations:
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Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Golda, Anna" sort="Golda, Anna" uniqKey="Golda A" first="Anna" last="Golda">Anna Golda</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Kabala, Wojciech" sort="Kabala, Wojciech" uniqKey="Kabala W" first="Wojciech" last="Kabala">Wojciech Kabala</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Burmistrz, Michal" sort="Burmistrz, Michal" uniqKey="Burmistrz M" first="Michał" last="Burmistrz">Michał Burmistrz</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Zdzalik, Michal" sort="Zdzalik, Michal" uniqKey="Zdzalik M" first="Michal" last="Zdzalik">Michal Zdzalik</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Nowak, Paulina" sort="Nowak, Paulina" uniqKey="Nowak P" first="Paulina" last="Nowak">Paulina Nowak</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Kedracka Krok, Sylwia" sort="Kedracka Krok, Sylwia" uniqKey="Kedracka Krok S" first="Sylwia" last="Kedracka-Krok">Sylwia Kedracka-Krok</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Zarebski, Miroslaw" sort="Zarebski, Miroslaw" uniqKey="Zarebski M" first="Mirosław" last="Zarebski">Mirosław Zarebski</name><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Dobrucki, Jerzy" sort="Dobrucki, Jerzy" uniqKey="Dobrucki J" first="Jerzy" last="Dobrucki">Jerzy Dobrucki</name><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Florek, Dominik" sort="Florek, Dominik" uniqKey="Florek D" first="Dominik" last="Florek">Dominik Florek</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Zeglen, Slawomir" sort="Zeglen, Slawomir" uniqKey="Zeglen S" first="Sławomir" last="Zeglen">Sławomir Zeglen</name><affiliation wicri:level="1"><nlm:aff id="aff005"><addr-line>Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Szpitalna 2, 41–800, Zabrze, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Szpitalna 2, 41–800, Zabrze</wicri:regionArea><wicri:noRegion>Zabrze</wicri:noRegion></affiliation></author><author><name sortKey="Wojarski, Jacek" sort="Wojarski, Jacek" uniqKey="Wojarski J" first="Jacek" last="Wojarski">Jacek Wojarski</name><affiliation wicri:level="1"><nlm:aff id="aff005"><addr-line>Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Szpitalna 2, 41–800, Zabrze, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Szpitalna 2, 41–800, Zabrze</wicri:regionArea><wicri:noRegion>Zabrze</wicri:noRegion></affiliation></author><author><name sortKey="Potempa, Jan" sort="Potempa, Jan" uniqKey="Potempa J" first="Jan" last="Potempa">Jan Potempa</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff006"><addr-line>Oral Health and Systemic Disease Research Group, School of Dentistry, University of Louisville, Louisville, KY, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Oral Health and Systemic Disease Research Group, School of Dentistry, University of Louisville, Louisville, KY</wicri:regionArea><wicri:noRegion>KY</wicri:noRegion></affiliation></author><author><name sortKey="Dubin, Grzegorz" sort="Dubin, Grzegorz" uniqKey="Dubin G" first="Grzegorz" last="Dubin">Grzegorz Dubin</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author><author><name sortKey="Pyrc, Krzysztof" sort="Pyrc, Krzysztof" uniqKey="Pyrc K" first="Krzysztof" last="Pyrc">Krzysztof Pyrc</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow</wicri:regionArea><wicri:noRegion>Krakow</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Human coronavirus (HCoV) NL63 was first described in 2004 and is associated with respiratory tract disease of varying severity. At the genetic and structural level, HCoV-NL63 is similar to other members of the <italic>Coronavirinae</italic> subfamily, especially human coronavirus 229E (HCoV-229E). Detailed analysis, however, reveals several unique features of the pathogen. The coronaviral nucleocapsid protein is abundantly present in infected cells. It is a multi-domain, multi-functional protein important for viral replication and a number of cellular processes. The aim of the present study was to characterize the HCoV-NL63 nucleocapsid protein. Biochemical analyses revealed that the protein shares characteristics with homologous proteins encoded in other coronaviral genomes, with the N-terminal domain responsible for nucleic acid binding and the C-terminal domain involved in protein oligomerization. Surprisingly, analysis of the subcellular localization of the N protein of HCoV-NL63 revealed that, differently than homologous proteins from other coronaviral species except for SARS-CoV, it is not present in the nucleus of infected or transfected cells. Furthermore, no significant alteration in cell cycle progression in cells expressing the protein was observed. This is in stark contrast with results obtained for other coronaviruses, except for the SARS-CoV.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="King, Amq" uniqKey="King A">AMQ King</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamre, D" uniqKey="Hamre D">D Hamre</name></author><author><name sortKey="Procknow, Jj" uniqKey="Procknow J">JJ Procknow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mcintosh, K" uniqKey="Mcintosh K">K McIntosh</name></author><author><name sortKey="Becker, Wb" uniqKey="Becker W">WB Becker</name></author><author><name sortKey="Chanock, Rm" uniqKey="Chanock R">RM Chanock</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tyrrell, Da" uniqKey="Tyrrell D">DA Tyrrell</name></author><author><name sortKey="Bynoe, Ml" uniqKey="Bynoe M">ML 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article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25700263</article-id><article-id pub-id-type="pmc">4336326</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0117833</article-id><article-id pub-id-type="publisher-id">PONE-D-14-22478</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>The Nucleocapsid Protein of Human Coronavirus NL63</article-title><alt-title alt-title-type="running-head">HCoV-NL63 N Protein</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zuwała</surname><given-names>Kaja</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Golda</surname><given-names>Anna</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kabala</surname><given-names>Wojciech</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Burmistrz</surname><given-names>Michał</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zdzalik</surname><given-names>Michal</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nowak</surname><given-names>Paulina</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kedracka-Krok</surname><given-names>Sylwia</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zarebski</surname><given-names>Mirosław</given-names></name><xref ref-type="aff" rid="aff004"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Dobrucki</surname><given-names>Jerzy</given-names></name><xref ref-type="aff" rid="aff004"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Florek</surname><given-names>Dominik</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zeglen</surname><given-names>Sławomir</given-names></name><xref ref-type="aff" rid="aff005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Wojarski</surname><given-names>Jacek</given-names></name><xref ref-type="aff" rid="aff005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Potempa</surname><given-names>Jan</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff006"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Dubin</surname><given-names>Grzegorz</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Pyrc</surname><given-names>Krzysztof</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref rid="cor001" ref-type="corresp">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></aff><aff id="aff003"><label>3</label><addr-line>Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30–387, Krakow, Poland</addr-line></aff><aff id="aff004"><label>4</label><addr-line>Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland</addr-line></aff><aff id="aff005"><label>5</label><addr-line>Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Szpitalna 2, 41–800, Zabrze, Poland</addr-line></aff><aff id="aff006"><label>6</label><addr-line>Oral Health and Systemic Disease Research Group, School of Dentistry, University of Louisville, Louisville, KY, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Darlix</surname><given-names>Jean-Luc EPH</given-names></name><role>Academic Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Institut National de la Santé et de la Recherche Médicale, FRANCE</addr-line></aff><author-notes><fn fn-type="COI-statement" id="coi001"><p><bold>Competing Interests: </bold>Krzysztof Pyrc serves as an active academic editor for PLOS ONE, but this does not alter the authors’ adherence to PLOS ONE Editorial policies and criteria.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: KZ AG GD SKK M. Zarebski M. Zdzalik KP. Performed the experiments: KZ AG WK MB PN SKK DF. Analyzed the data: KP GD. Contributed reagents/materials/analysis tools: SZ JW. Wrote the paper: KZ GD JD JP KP.</p></fn><corresp id="cor001">* E-mail: <email>k.a.pyrc@uj.edu.pl</email></corresp></author-notes><pub-date pub-type="epub"><day>20</day><month>2</month><year>2015</year></pub-date><pub-date pub-type="collection"><year>2015</year></pub-date><volume>10</volume><issue>2</issue><elocation-id>e0117833</elocation-id><history><date date-type="received"><day>20</day><month>5</month><year>2014</year></date><date date-type="accepted"><day>2</day><month>1</month><year>2015</year></date></history><permissions><license xlink:href="https://creativecommons.org/publicdomain/zero/1.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="pone.0117833.pdf"></self-uri><abstract><p>Human coronavirus (HCoV) NL63 was first described in 2004 and is associated with respiratory tract disease of varying severity. At the genetic and structural level, HCoV-NL63 is similar to other members of the <italic>Coronavirinae</italic> subfamily, especially human coronavirus 229E (HCoV-229E). Detailed analysis, however, reveals several unique features of the pathogen. The coronaviral nucleocapsid protein is abundantly present in infected cells. It is a multi-domain, multi-functional protein important for viral replication and a number of cellular processes. The aim of the present study was to characterize the HCoV-NL63 nucleocapsid protein. Biochemical analyses revealed that the protein shares characteristics with homologous proteins encoded in other coronaviral genomes, with the N-terminal domain responsible for nucleic acid binding and the C-terminal domain involved in protein oligomerization. Surprisingly, analysis of the subcellular localization of the N protein of HCoV-NL63 revealed that, differently than homologous proteins from other coronaviral species except for SARS-CoV, it is not present in the nucleus of infected or transfected cells. Furthermore, no significant alteration in cell cycle progression in cells expressing the protein was observed. This is in stark contrast with results obtained for other coronaviruses, except for the SARS-CoV.</p></abstract><funding-group><funding-statement>This work was supported by the LIDER grant from the National Centre for Research and Development (Lider/27/55/L-2/10/2011) (KP) and Iuventus Plus grant from the Ministry of Science and Higher Education, Poland (IP 2010 033870 and IP2011 044371) (KP) and grants from the National Science Center (UMO-2012/05/B/NZ4/02428 (SKK), UMO-2011/01/D/NZ1/01169 (GD) and UMO-2012/07/E/NZ6/01712 (KP)). The Faculty of Biochemistry, Biophysics and Biotechnology of the Jagiellonian University is a beneficiary of the structural funds from the European Union (grant No: POIG.02.01.00-12-064/08 – “Molecular biotechnology for health”). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><fig-count count="7"></fig-count><table-count count="3"></table-count><page-count count="23"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper and its Supporting Information files.</p></notes></front></pmc><affiliations><list><country><li>Pologne</li><li>États-Unis</li></country></list><tree><country name="Pologne"><noRegion><name sortKey="Zuwala, Kaja" sort="Zuwala, Kaja" uniqKey="Zuwala K" first="Kaja" last="Zuwała">Kaja Zuwała</name></noRegion><name sortKey="Burmistrz, Michal" sort="Burmistrz, Michal" uniqKey="Burmistrz M" first="Michał" last="Burmistrz">Michał Burmistrz</name><name sortKey="Dobrucki, Jerzy" sort="Dobrucki, Jerzy" uniqKey="Dobrucki J" first="Jerzy" last="Dobrucki">Jerzy Dobrucki</name><name sortKey="Dubin, Grzegorz" sort="Dubin, Grzegorz" uniqKey="Dubin G" first="Grzegorz" last="Dubin">Grzegorz Dubin</name><name sortKey="Dubin, Grzegorz" sort="Dubin, Grzegorz" uniqKey="Dubin G" first="Grzegorz" last="Dubin">Grzegorz Dubin</name><name sortKey="Florek, Dominik" sort="Florek, Dominik" uniqKey="Florek D" first="Dominik" last="Florek">Dominik Florek</name><name sortKey="Golda, Anna" sort="Golda, Anna" uniqKey="Golda A" first="Anna" last="Golda">Anna Golda</name><name sortKey="Kabala, Wojciech" sort="Kabala, Wojciech" uniqKey="Kabala W" first="Wojciech" last="Kabala">Wojciech Kabala</name><name sortKey="Kabala, Wojciech" sort="Kabala, Wojciech" uniqKey="Kabala W" first="Wojciech" last="Kabala">Wojciech Kabala</name><name sortKey="Kedracka Krok, Sylwia" sort="Kedracka Krok, Sylwia" uniqKey="Kedracka Krok S" first="Sylwia" last="Kedracka-Krok">Sylwia Kedracka-Krok</name><name sortKey="Kedracka Krok, Sylwia" sort="Kedracka Krok, Sylwia" uniqKey="Kedracka Krok S" first="Sylwia" last="Kedracka-Krok">Sylwia Kedracka-Krok</name><name sortKey="Nowak, Paulina" sort="Nowak, Paulina" uniqKey="Nowak P" first="Paulina" last="Nowak">Paulina Nowak</name><name sortKey="Potempa, Jan" sort="Potempa, Jan" uniqKey="Potempa J" first="Jan" last="Potempa">Jan Potempa</name><name sortKey="Pyrc, Krzysztof" sort="Pyrc, Krzysztof" uniqKey="Pyrc K" first="Krzysztof" last="Pyrc">Krzysztof Pyrc</name><name sortKey="Pyrc, Krzysztof" sort="Pyrc, Krzysztof" uniqKey="Pyrc K" first="Krzysztof" last="Pyrc">Krzysztof Pyrc</name><name sortKey="Wojarski, Jacek" sort="Wojarski, Jacek" uniqKey="Wojarski J" first="Jacek" last="Wojarski">Jacek Wojarski</name><name sortKey="Zarebski, Miroslaw" sort="Zarebski, Miroslaw" uniqKey="Zarebski M" first="Mirosław" last="Zarebski">Mirosław Zarebski</name><name sortKey="Zdzalik, Michal" sort="Zdzalik, Michal" uniqKey="Zdzalik M" first="Michal" last="Zdzalik">Michal Zdzalik</name><name sortKey="Zeglen, Slawomir" sort="Zeglen, Slawomir" uniqKey="Zeglen S" first="Sławomir" last="Zeglen">Sławomir Zeglen</name></country><country name="États-Unis"><noRegion><name sortKey="Potempa, Jan" sort="Potempa, Jan" uniqKey="Potempa J" first="Jan" last="Potempa">Jan Potempa</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|clé= PMC:4336326
|texte= The Nucleocapsid Protein of Human Coronavirus NL63
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:25700263" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a CovidV1
| This area was generated with Dilib version V0.6.33. |  |