Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of Heliothis virescens ascovirus 3 h (HvAV-3h)
Identifieur interne : 000171 ( Pmc/Checkpoint ); précédent : 000170; suivant : 000172Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of Heliothis virescens ascovirus 3 h (HvAV-3h)
Auteurs : Zi-Shu Chen ; Ning-Ning Han ; Jian-Hong Li ; Guo-Hua Huang ; Hu WanSource :
- Scientific Reports [ 2045-2322 ] ; 2017.
Abstract
Ascoviruses are double-stranded DNA viruses that mainly infect noctuid larvae, and are transmitted by the parasitoid wasp
Url:
DOI: 10.1038/s41598-017-07684-w
PubMed: 28765578
PubMed Central: 5539149
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of <italic>Heliothis virescens</italic> ascovirus 3 h (HvAV-3h)</title><author><name sortKey="Chen, Zi Shu" sort="Chen, Zi Shu" uniqKey="Chen Z" first="Zi-Shu" last="Chen">Zi-Shu Chen</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.257160.7</institution-id><institution>Institute of Virology, College of Plant protection,</institution><institution>Hunan Agricultural University,</institution></institution-wrap>Changsha, 410128 Hunan China</nlm:aff><wicri:noCountry code="subfield">410128 Hunan China</wicri:noCountry></affiliation></author><author><name sortKey="Han, Ning Ning" sort="Han, Ning Ning" uniqKey="Han N" first="Ning-Ning" last="Han">Ning-Ning Han</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation></author><author><name sortKey="Li, Jian Hong" sort="Li, Jian Hong" uniqKey="Li J" first="Jian-Hong" last="Li">Jian-Hong Li</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation></author><author><name sortKey="Huang, Guo Hua" sort="Huang, Guo Hua" uniqKey="Huang G" first="Guo-Hua" last="Huang">Guo-Hua Huang</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.257160.7</institution-id><institution>Institute of Virology, College of Plant protection,</institution><institution>Hunan Agricultural University,</institution></institution-wrap>Changsha, 410128 Hunan China</nlm:aff><wicri:noCountry code="subfield">410128 Hunan China</wicri:noCountry></affiliation></author><author><name sortKey="Wan, Hu" sort="Wan, Hu" uniqKey="Wan H" first="Hu" last="Wan">Hu Wan</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28765578</idno><idno type="pmc">5539149</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539149</idno><idno type="RBID">PMC:5539149</idno><idno type="doi">10.1038/s41598-017-07684-w</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000153</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000153</idno><idno type="wicri:Area/Pmc/Curation">000153</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000153</idno><idno type="wicri:Area/Pmc/Checkpoint">000171</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000171</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of <italic>Heliothis virescens</italic> ascovirus 3 h (HvAV-3h)</title><author><name sortKey="Chen, Zi Shu" sort="Chen, Zi Shu" uniqKey="Chen Z" first="Zi-Shu" last="Chen">Zi-Shu Chen</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.257160.7</institution-id><institution>Institute of Virology, College of Plant protection,</institution><institution>Hunan Agricultural University,</institution></institution-wrap>Changsha, 410128 Hunan China</nlm:aff><wicri:noCountry code="subfield">410128 Hunan China</wicri:noCountry></affiliation></author><author><name sortKey="Han, Ning Ning" sort="Han, Ning Ning" uniqKey="Han N" first="Ning-Ning" last="Han">Ning-Ning Han</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation></author><author><name sortKey="Li, Jian Hong" sort="Li, Jian Hong" uniqKey="Li J" first="Jian-Hong" last="Li">Jian-Hong Li</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation></author><author><name sortKey="Huang, Guo Hua" sort="Huang, Guo Hua" uniqKey="Huang G" first="Guo-Hua" last="Huang">Guo-Hua Huang</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.257160.7</institution-id><institution>Institute of Virology, College of Plant protection,</institution><institution>Hunan Agricultural University,</institution></institution-wrap>Changsha, 410128 Hunan China</nlm:aff><wicri:noCountry code="subfield">410128 Hunan China</wicri:noCountry></affiliation></author><author><name sortKey="Wan, Hu" sort="Wan, Hu" uniqKey="Wan H" first="Hu" last="Wan">Hu Wan</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Ascoviruses are double-stranded DNA viruses that mainly infect noctuid larvae, and are transmitted by the parasitoid wasp <italic>Microplitis similis</italic> Lyle. Ascovirus-parasitoids wasp-noctuid insects constitute the dissemination system. Selection of suitable reference genes for the dissemination system could play an important role in elucidating the pathogenic molecular mechanisms of ascovirus. Unfortunately, such studies on potential reference genes in the dissemination system of ascoviruses are lacking. In the present study, we evaluated 11 candidate reference genes: <italic>β-actin1</italic> (<italic>ACT1</italic>), <italic>β-actin2</italic> (<italic>ACT2</italic>), <italic>elongation factor 1</italic> (<italic>EF1</italic>), <italic>elongation factor 2</italic> (<italic>EF2</italic>), <italic>ribosomal protein L10</italic> (<italic>L10</italic>), <italic>ribosomal protein L17A</italic> (<italic>L17A</italic>), <italic>superoxide dismutase</italic> (<italic>SOD</italic>), <italic>28S ribosome</italic> (<italic>28S</italic>), <italic>Tubulin</italic> (<italic>TUB</italic>) and <italic>18S ribosome</italic> (<italic>18S</italic>). The samples were originally from various virus concentrations and points-in-time of experimental treatments using RefFinder and four algorithms. The results showed that <italic>EF1</italic> was the most stable internal gene in <italic>S. exigua</italic> and <italic>M. similis</italic> and that <italic>EF2</italic> was the most stable in the IOZCAS-Spex-II-A cell line, and the stability of reference genes were confirmed via the expression levels of two inhibitor of apoptosis-like (<italic>iap</italic>-like) genes from <italic>Heliothis virescens</italic> ascovirus 3 h (HvAV-3h). This study provides a crucial basis for future research that explores the molecular mechanisms of the pathogenesis of ascoviruses.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Stasiak, K" uniqKey="Stasiak K">K Stasiak</name></author><author><name sortKey="Renault, S" uniqKey="Renault S">S Renault</name></author><author><name sortKey="Demattei, Mv" uniqKey="Demattei M">MV Demattei</name></author><author><name sortKey="Bigot, Y" uniqKey="Bigot Y">Y Bigot</name></author><author><name sortKey="Federici, Ba" uniqKey="Federici B">BA Federici</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Asgari, S" uniqKey="Asgari S">S Asgari</name></author><author><name sortKey="Davis, J" uniqKey="Davis J">J Davis</name></author><author><name sortKey="Wood, D" uniqKey="Wood D">D Wood</name></author><author><name sortKey="Wilson, P" uniqKey="Wilson P">P Wilson</name></author><author><name sortKey="Mcgrath, A" uniqKey="Mcgrath A">A McGrath</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheng, Xw" uniqKey="Cheng X">XW Cheng</name></author><author><name sortKey="Carner, Gr" uniqKey="Carner G">GR Carner</name></author><author><name sortKey="Brown, Tm" uniqKey="Brown T">TM Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, Gh" uniqKey="Huang G">GH Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, Gh" uniqKey="Huang G">GH Huang</name></author><author><name sortKey="Hou, Dh" uniqKey="Hou D">DH Hou</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Cheng, Xw" uniqKey="Cheng X">XW Cheng</name></author><author><name sortKey="Hu, Z" uniqKey="Hu Z">Z Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bideshi, Dk" uniqKey="Bideshi D">DK Bideshi</name></author><author><name sortKey="Tan, Y" uniqKey="Tan Y">Y Tan</name></author><author><name sortKey="Bigot, Y" uniqKey="Bigot Y">Y Bigot</name></author><author><name sortKey="Federici, Ba" uniqKey="Federici B">BA Federici</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Sj" uniqKey="Li S">SJ Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, J" uniqKey="Hu J">J Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valasek, Ma" uniqKey="Valasek M">MA Valasek</name></author><author><name sortKey="Repa, Jj" uniqKey="Repa J">JJ Repa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fleige, S" uniqKey="Fleige S">S Fleige</name></author><author><name sortKey="Pfaffl, Mw" uniqKey="Pfaffl M">MW Pfaffl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huggett, J" uniqKey="Huggett J">J Huggett</name></author><author><name sortKey="Dheda, K" uniqKey="Dheda K">K Dheda</name></author><author><name sortKey="Bustin, S" uniqKey="Bustin S">S Bustin</name></author><author><name sortKey="Zumla, A" uniqKey="Zumla A">A Zumla</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Crespo, D" uniqKey="Garcia Crespo D">D Garcia-Crespo</name></author><author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name></author><author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dheda, K" uniqKey="Dheda K">K Dheda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jain, M" uniqKey="Jain M">M Jain</name></author><author><name sortKey="Nijhawan, A" uniqKey="Nijhawan A">A Nijhawan</name></author><author><name sortKey="Tyagi, Ak" uniqKey="Tyagi A">AK Tyagi</name></author><author><name sortKey="Khurana, Jp" uniqKey="Khurana J">JP Khurana</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Radoni, A" uniqKey="Radoni A">A Radonić</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Salem, Tz" uniqKey="Salem T">TZ Salem</name></author><author><name sortKey="Allam, Wr" uniqKey="Allam W">WR Allam</name></author><author><name sortKey="Thiem, Sm" uniqKey="Thiem S">SM Thiem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xue, Jl" uniqKey="Xue J">JL Xue</name></author><author><name sortKey="Salem, Tz" uniqKey="Salem T">TZ Salem</name></author><author><name sortKey="Turney, Cm" uniqKey="Turney C">CM Turney</name></author><author><name sortKey="Cheng, Xw" uniqKey="Cheng X">XW Cheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Niu, J" uniqKey="Niu J">J Niu</name></author><author><name sortKey="Cappelle, K" uniqKey="Cappelle K">K Cappelle</name></author><author><name sortKey="De Miranda, Jr" uniqKey="De Miranda J">JR de Miranda</name></author><author><name sortKey="Smagghe, G" uniqKey="Smagghe G">G Smagghe</name></author><author><name sortKey="Meeus, I" uniqKey="Meeus I">I Meeus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Radoni, A" uniqKey="Radoni A">A Radonić</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, S" uniqKey="Zhang S">S Zhang</name></author><author><name sortKey="Zhang, Yj" uniqKey="Zhang Y">YJ Zhang</name></author><author><name sortKey="Su, Hh" uniqKey="Su H">HH Su</name></author><author><name sortKey="Gao, Xw" uniqKey="Gao X">XW Gao</name></author><author><name sortKey="Guo, Yy" uniqKey="Guo Y">YY Guo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deveraux, Ql" uniqKey="Deveraux Q">QL Deveraux</name></author><author><name sortKey="Reed, Jc" uniqKey="Reed J">JC Reed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Crook, Ne" uniqKey="Crook N">NE Crook</name></author><author><name sortKey="Clem, Rj" uniqKey="Clem R">RJ Clem</name></author><author><name sortKey="Miller, Lk" uniqKey="Miller L">LK Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Clem, Rj" uniqKey="Clem R">RJ Clem</name></author><author><name sortKey="Miller, Lk" uniqKey="Miller L">LK Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, X" uniqKey="Jiang X">X Jiang</name></author><author><name sortKey="Luo, L" uniqKey="Luo L">L Luo</name></author><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Sj" uniqKey="Li S">SJ Li</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Reed, Lj" uniqKey="Reed L">LJ Reed</name></author><author><name sortKey="Muench, Ha" uniqKey="Muench H">HA Muench</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tillman, Pg" uniqKey="Tillman P">PG Tillman</name></author><author><name sortKey="Styer, El" uniqKey="Styer E">EL Styer</name></author><author><name sortKey="Hamm, Jj" uniqKey="Hamm J">JJ Hamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ma, Ks" uniqKey="Ma K">KS Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, X" uniqKey="Zhu X">X Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pfaffl, Mw" uniqKey="Pfaffl M">MW Pfaffl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Radoni, A" uniqKey="Radoni A">A Radonić</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vandesompele, J" uniqKey="Vandesompele J">J Vandesompele</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Andersen, Cl" uniqKey="Andersen C">CL Andersen</name></author><author><name sortKey="Jensen, Jl" uniqKey="Jensen J">JL Jensen</name></author><author><name sortKey=" Rntoft, Tf" uniqKey=" Rntoft T">TF Ørntoft</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pfaffl, Mw" uniqKey="Pfaffl M">MW Pfaffl</name></author><author><name sortKey="Tichopad, A" uniqKey="Tichopad A">A Tichopad</name></author><author><name sortKey="Prgomet, C" uniqKey="Prgomet C">C Prgomet</name></author><author><name sortKey="Neuvians, Tp" uniqKey="Neuvians T">TP Neuvians</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Silver, N" uniqKey="Silver N">N Silver</name></author><author><name sortKey="Best, S" uniqKey="Best S">S Best</name></author><author><name sortKey="Jiang, J" uniqKey="Jiang J">J Jiang</name></author><author><name sortKey="Thein, Sl" uniqKey="Thein S">SL Thein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Livak, Kj" uniqKey="Livak K">KJ Livak</name></author><author><name sortKey="Schmittgen, Td" uniqKey="Schmittgen T">TD Schmittgen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bustin, Sa" uniqKey="Bustin S">SA Bustin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Teng, X" uniqKey="Teng X">X Teng</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author><author><name sortKey="He, G" uniqKey="He G">G He</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L Yang</name></author><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brito, Dv" uniqKey="Brito D">DV Brito</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ma, L" uniqKey="Ma L">L Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Hl" uniqKey="Chen H">HL Chen</name></author><author><name sortKey="Zhang, Hy" uniqKey="Zhang H">HY Zhang</name></author><author><name sortKey="Throne, Je" uniqKey="Throne J">JE Throne</name></author><author><name sortKey="Zhu, Ky" uniqKey="Zhu K">KY Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Asgari, S" uniqKey="Asgari S">S Asgari</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, H" uniqKey="Zhang H">H Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hussain, M" uniqKey="Hussain M">M Hussain</name></author><author><name sortKey="Asgari, S" uniqKey="Asgari S">S Asgari</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id><journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id><journal-title-group><journal-title>Scientific Reports</journal-title></journal-title-group><issn pub-type="epub">2045-2322</issn><publisher><publisher-name>Nature Publishing Group UK</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28765578</article-id><article-id pub-id-type="pmc">5539149</article-id><article-id pub-id-type="publisher-id">7684</article-id><article-id pub-id-type="doi">10.1038/s41598-017-07684-w</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of <italic>Heliothis virescens</italic> ascovirus 3 h (HvAV-3h)</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Zi-Shu</given-names></name><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Han</surname><given-names>Ning-Ning</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Jian-Hong</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Guo-Hua</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Wan</surname><given-names>Hu</given-names></name><address><email>huwan@mail.hzau.edu.cn</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="GRID">grid.257160.7</institution-id><institution>Institute of Virology, College of Plant protection,</institution><institution>Hunan Agricultural University,</institution></institution-wrap>Changsha, 410128 Hunan China</aff></contrib-group><pub-date pub-type="epub"><day>1</day><month>8</month><year>2017</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>8</month><year>2017</year></pub-date><pub-date pub-type="collection"><year>2017</year></pub-date><volume>7</volume><elocation-id>7045</elocation-id><history><date date-type="received"><day>22</day><month>12</month><year>2016</year></date><date date-type="accepted"><day>30</day><month>6</month><year>2017</year></date></history><permissions><copyright-statement>© The Author(s) 2017</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold> This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>.</license-p></license></permissions><abstract id="Abs1"><p id="Par1">Ascoviruses are double-stranded DNA viruses that mainly infect noctuid larvae, and are transmitted by the parasitoid wasp <italic>Microplitis similis</italic> Lyle. Ascovirus-parasitoids wasp-noctuid insects constitute the dissemination system. Selection of suitable reference genes for the dissemination system could play an important role in elucidating the pathogenic molecular mechanisms of ascovirus. Unfortunately, such studies on potential reference genes in the dissemination system of ascoviruses are lacking. In the present study, we evaluated 11 candidate reference genes: <italic>β-actin1</italic> (<italic>ACT1</italic>), <italic>β-actin2</italic> (<italic>ACT2</italic>), <italic>elongation factor 1</italic> (<italic>EF1</italic>), <italic>elongation factor 2</italic> (<italic>EF2</italic>), <italic>ribosomal protein L10</italic> (<italic>L10</italic>), <italic>ribosomal protein L17A</italic> (<italic>L17A</italic>), <italic>superoxide dismutase</italic> (<italic>SOD</italic>), <italic>28S ribosome</italic> (<italic>28S</italic>), <italic>Tubulin</italic> (<italic>TUB</italic>) and <italic>18S ribosome</italic> (<italic>18S</italic>). The samples were originally from various virus concentrations and points-in-time of experimental treatments using RefFinder and four algorithms. The results showed that <italic>EF1</italic> was the most stable internal gene in <italic>S. exigua</italic> and <italic>M. similis</italic> and that <italic>EF2</italic> was the most stable in the IOZCAS-Spex-II-A cell line, and the stability of reference genes were confirmed via the expression levels of two inhibitor of apoptosis-like (<italic>iap</italic>-like) genes from <italic>Heliothis virescens</italic> ascovirus 3 h (HvAV-3h). This study provides a crucial basis for future research that explores the molecular mechanisms of the pathogenesis of ascoviruses.</p></abstract><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2017</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Chen, Zi Shu" sort="Chen, Zi Shu" uniqKey="Chen Z" first="Zi-Shu" last="Chen">Zi-Shu Chen</name><name sortKey="Han, Ning Ning" sort="Han, Ning Ning" uniqKey="Han N" first="Ning-Ning" last="Han">Ning-Ning Han</name><name sortKey="Huang, Guo Hua" sort="Huang, Guo Hua" uniqKey="Huang G" first="Guo-Hua" last="Huang">Guo-Hua Huang</name><name sortKey="Li, Jian Hong" sort="Li, Jian Hong" uniqKey="Li J" first="Jian-Hong" last="Li">Jian-Hong Li</name><name sortKey="Wan, Hu" sort="Wan, Hu" uniqKey="Wan H" first="Hu" last="Wan">Hu Wan</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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