CovidV1, PascalFrancis, Curation, bibRecord, 000006

Development and comparison of the real-time amplification based methods: NASBA-Beacon, RT-PCR Taqman and RT-PCR hybridization probe assays: For the qualitative detection of SARS coronavirus

Identifieur interne : 000006 ( PascalFrancis/Curation ); précédent : 000005; suivant : 000007

Development and comparison of the real-time amplification based methods: NASBA-Beacon, RT-PCR Taqman and RT-PCR hybridization probe assays: For the qualitative detection of SARS coronavirus

Auteurs : Wasun Chantratita [Thaïlande] ; Wiroj Pongtanapisit [Thaïlande] ; Wantanich Piroj [Thaïlande] ; Chutatip Srichunrasmi [Thaïlande] ; Somying Seesuai [Thaïlande]

Source :

Southeast Asian journal of tropical medicine and public health [ 0125-1562 ] ; 2004.

RBID : Pascal:04-0591783

Descripteurs français

Pascal (Inist)
- Etude comparative, Réaction chaîne polymérase RT, Sonde, Syndrome respiratoire aigu sévère, Coronavirus, Médecine tropicale.

English descriptors

KwdEn :
- Comparative study, Coronavirus, Probe, Reverse transcription polymerase chain reaction, Severe acute respiratory syndrome, Tropical medicine.

Abstract

The aim of this study was to develop a rapid, sensitive and robust procedure for the qualitative detection of SARS coronavirus RNA. Three unique detection formats were developed for real-time RNA amplification assays: a post amplification detection step with a virus-specific internal capture probe based on Taqman (RT-PCR TaqMan assay), hybridization probe (RT-PCR hybridization probe assay) and a real-time assay with virus-specific molecular beacon probes (NASBA-Beacon assay). The analytical sensitivity or reproducibility of the test results among those three assays was compared. All assays yielded results by detecting SARS coronavirus targeting the BNI-1 region in less than 2 hours. RNA detection by all the formats was unaffected by the presence of human sputum. The limits of detection were at least 10 copies of input RNA for both RT-PCR formats (RT-PCR TaqMan and RT-PCR hybridization probe assays), while the NASBA-Beacon assay could detect as little as 1 copy per reaction, with high reproducibility of the coefficient of variation (CV) of <10. These results demonstrate that real-time NASBA provides a rapid and sensitive alternative to RT-PCR for the routine qualitative assay of sputum for SARS corona viral RNA detection.

A01	`01`	`1`		`@0 0125-1562`
A02	`01`			`@0 SJTMAK`
A03		`1`		`@0 Southeast asian j. trop. med. public health`
A05				`@2 35`
A06				`@2 3`
A08	`01`	`1`	`ENG`	`@1 Development and comparison of the real-time amplification based methods: NASBA-Beacon, RT-PCR Taqman and RT-PCR hybridization probe assays: For the qualitative detection of SARS coronavirus`
A11	`01`	`1`		`@1 CHANTRATITA (Wasun)`
A11	`02`	`1`		`@1 PONGTANAPISIT (Wiroj)`
A11	`03`	`1`		`@1 PIROJ (Wantanich)`
A11	`04`	`1`		`@1 SRICHUNRASMI (Chutatip)`
A11	`05`	`1`		`@1 SEESUAI (Somying)`
A14	`01`			`@1 Virology and Molecular Microbiology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University @2 Bangkok @3 THA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut.`
A20				`@1 623-629`
A21				`@1 2004`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 19778 @5 354000122499500230`
A44				`@0 0000 @1 © 2004 INIST-CNRS. All rights reserved.`
A45				`@0 11 ref.`
A47	`01`	`1`		`@0 04-0591783`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Southeast Asian journal of tropical medicine and public health`
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C01	`01`		`ENG`	@0 The aim of this study was to develop a rapid, sensitive and robust procedure for the qualitative detection of SARS coronavirus RNA. Three unique detection formats were developed for real-time RNA amplification assays: a post amplification detection step with a virus-specific internal capture probe based on Taqman (RT-PCR TaqMan assay), hybridization probe (RT-PCR hybridization probe assay) and a real-time assay with virus-specific molecular beacon probes (NASBA-Beacon assay). The analytical sensitivity or reproducibility of the test results among those three assays was compared. All assays yielded results by detecting SARS coronavirus targeting the BNI-1 region in less than 2 hours. RNA detection by all the formats was unaffected by the presence of human sputum. The limits of detection were at least 10 copies of input RNA for both RT-PCR formats (RT-PCR TaqMan and RT-PCR hybridization probe assays), while the NASBA-Beacon assay could detect as little as 1 copy per reaction, with high reproducibility of the coefficient of variation (CV) of <10. These results demonstrate that real-time NASBA provides a rapid and sensitive alternative to RT-PCR for the routine qualitative assay of sputum for SARS corona viral RNA detection.
C02	`01`	`X`		`@0 002B01`
C03	`01`	`X`	`FRE`	`@0 Etude comparative @5 02`
C03	`01`	`X`	`ENG`	`@0 Comparative study @5 02`
C03	`01`	`X`	`SPA`	`@0 Estudio comparativo @5 02`
C03	`02`	`X`	`FRE`	`@0 Réaction chaîne polymérase RT @5 03`
C03	`02`	`X`	`ENG`	`@0 Reverse transcription polymerase chain reaction @5 03`
C03	`02`	`X`	`SPA`	`@0 Reacción cadena polimerasa transcripción inversa @5 03`
C03	`03`	`X`	`FRE`	`@0 Sonde @5 05`
C03	`03`	`X`	`ENG`	`@0 Probe @5 05`
C03	`03`	`X`	`SPA`	`@0 Sonda @5 05`
C03	`04`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 06`
C03	`04`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 06`
C03	`04`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 06`
C03	`05`	`X`	`FRE`	`@0 Coronavirus @2 NW @5 08`
C03	`05`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 08`
C03	`05`	`X`	`SPA`	`@0 Coronavirus @2 NW @5 08`
C03	`06`	`X`	`FRE`	`@0 Médecine tropicale @5 09`
C03	`06`	`X`	`ENG`	`@0 Tropical medicine @5 09`
C03	`06`	`X`	`SPA`	`@0 Medicina tropical @5 09`
C07	`01`	`X`	`FRE`	`@0 Virose @2 NM`
C07	`01`	`X`	`ENG`	`@0 Viral disease @2 NM`
C07	`01`	`X`	`SPA`	`@0 Virosis @2 NM`
C07	`02`	`X`	`FRE`	`@0 Infection @2 NM`
C07	`02`	`X`	`ENG`	`@0 Infection @2 NM`
C07	`02`	`X`	`SPA`	`@0 Infección @2 NM`
C07	`03`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`03`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`03`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`04`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`04`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`04`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`05`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`05`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`05`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`06`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 37`
C07	`06`	`X`	`ENG`	`@0 Respiratory disease @5 37`
C07	`06`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 37`
C07	`07`	`X`	`FRE`	`@0 Poumon pathologie @5 38`
C07	`07`	`X`	`ENG`	`@0 Lung disease @5 38`
C07	`07`	`X`	`SPA`	`@0 Pulmón patología @5 38`
N21				`@1 341`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

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Le document en format XML

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<front><div type="abstract" xml:lang="en">The aim of this study was to develop a rapid, sensitive and robust procedure for the qualitative detection of SARS coronavirus RNA. Three unique detection formats were developed for real-time RNA amplification assays: a post amplification detection step with a virus-specific internal capture probe based on Taqman (RT-PCR TaqMan assay), hybridization probe (RT-PCR hybridization probe assay) and a real-time assay with virus-specific molecular beacon probes (NASBA-Beacon assay). The analytical sensitivity or reproducibility of the test results among those three assays was compared. All assays yielded results by detecting SARS coronavirus targeting the BNI-1 region in less than 2 hours. RNA detection by all the formats was unaffected by the presence of human sputum. The limits of detection were at least 10 copies of input RNA for both RT-PCR formats (RT-PCR TaqMan and RT-PCR hybridization probe assays), while the NASBA-Beacon assay could detect as little as 1 copy per reaction, with high reproducibility of the coefficient of variation (CV) of <10. These results demonstrate that real-time NASBA provides a rapid and sensitive alternative to RT-PCR for the routine qualitative assay of sputum for SARS corona viral RNA detection.</div>
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<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Tropical medicine</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Medicina tropical</s0>
<s5>09</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Virose</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Viral disease</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Virosis</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Infección</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>37</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>37</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>37</s5>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>38</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>38</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>38</s5>
</fC07>
<fN21><s1>341</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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   |texte=   Development and comparison of the real-time amplification based methods: NASBA-Beacon, RT-PCR Taqman and RT-PCR hybridization probe assays: For the qualitative detection of SARS coronavirus
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Development and comparison of the real-time amplification based methods: NASBA-Beacon, RT-PCR Taqman and RT-PCR hybridization probe assays: For the qualitative detection of SARS coronavirus

Development and comparison of the real-time amplification based methods: NASBA-Beacon, RT-PCR Taqman and RT-PCR hybridization probe assays: For the qualitative detection of SARS coronavirus

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