Targeting non-human corona viruses to human cancer cells using a bispecific single-chain antibody
Identifieur interne : 000046 ( PascalFrancis/Corpus ); précédent : 000045; suivant : 000047Targeting non-human corona viruses to human cancer cells using a bispecific single-chain antibody
Auteurs : T. Würdinger ; M. H. Verheije ; M. Raaben ; B. J. Bosch ; C. A. M. De Haan ; V. W. Van Beusechem ; P. J. M. Rottier ; W. R. GerritsenSource :
- Gene therapy : (Basingstoke) [ 0969-7128 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
To explore the potential of using non-human coronaviruses for cancer therapy, we first established their ability to kill human tumor cells. We found that the feline infectious peritonitis virus (FIPV) and a felinized murine hepatitis virus (fMHV), both normally incapable of infecting human cells, could rapidly and effectively kill human cancer cells artificially expressing the feline coronavirus receptor aminopeptidase N. Also 3-D multilayer tumor spheroids established from such cells were effectively eradicated. Next, we investigated whether FIPV and fMHV could be targeted to human cancer cells by constructing a bispecific single-chain antibody directed on the one hand against the feline coronavirus spike protein - responsible for receptor binding and subsequent cell entry through virus-cell membrane fusion - and on the other hand against the human epidermal growth factor receptor (EGFR). The targeting antibody mediated specific infection of EGFR-expressing human cancer cells by both coronaviruses. Furthermore, in the presence of the targeting antibody, infected cancer cells formed syncytia typical of productive coronavirus infection. By their potent cytotoxicity, the selective targeting of non-human coronaviruses to human cancer cells provides a rationale for further investigations into the use of these viruses as anticancer agents.
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Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 05-0422331 INIST |
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ET : | Targeting non-human corona viruses to human cancer cells using a bispecific single-chain antibody |
AU : | WÜRDINGER (T.); VERHEIJE (M. H.); RAABEN (M.); BOSCH (B. J.); DE HAAN (C. A. M.); VAN BEUSECHEM (V. W.); ROTTIER (P. J. M.); GERRITSEN (W. R.) |
AF : | Virology Division, Department of Infectious Diseases and Immunology, Utrecht University/Utrecht/Pays-Bas (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 7 aut.); Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center/Amsterdam/Pays-Bas (1 aut., 2 aut., 6 aut., 8 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Gene therapy : (Basingstoke); ISSN 0969-7128; Royaume-Uni; Da. 2005; Vol. 12; No. 18; Pp. 1394-1404; Bibl. 42 ref. |
LA : | Anglais |
EA : | To explore the potential of using non-human coronaviruses for cancer therapy, we first established their ability to kill human tumor cells. We found that the feline infectious peritonitis virus (FIPV) and a felinized murine hepatitis virus (fMHV), both normally incapable of infecting human cells, could rapidly and effectively kill human cancer cells artificially expressing the feline coronavirus receptor aminopeptidase N. Also 3-D multilayer tumor spheroids established from such cells were effectively eradicated. Next, we investigated whether FIPV and fMHV could be targeted to human cancer cells by constructing a bispecific single-chain antibody directed on the one hand against the feline coronavirus spike protein - responsible for receptor binding and subsequent cell entry through virus-cell membrane fusion - and on the other hand against the human epidermal growth factor receptor (EGFR). The targeting antibody mediated specific infection of EGFR-expressing human cancer cells by both coronaviruses. Furthermore, in the presence of the targeting antibody, infected cancer cells formed syncytia typical of productive coronavirus infection. By their potent cytotoxicity, the selective targeting of non-human coronaviruses to human cancer cells provides a rationale for further investigations into the use of these viruses as anticancer agents. |
CC : | 002A31D01D; 002B27D03; 215 |
FD : | Ciblage; Homme; Anticorps bispécifique; Anticorps simple chaîne; Thérapie génique; Tumeur maligne; Coronavirus; Récepteur EGFR |
FG : | Coronaviridae; Nidovirales; Virus |
ED : | Targeting; Human; Bispecific antibody; Single chain antibody; Gene therapy; Malignant tumor; Coronavirus; Epidermal growth factor receptor |
EG : | Coronaviridae; Nidovirales; Virus |
SD : | Blancado; Hombre; Anticuerpo biespecífico; Anticuerpo simple cadena; Terapia génica; Tumor maligno; Coronavirus; Receptor EGFR |
LO : | INIST-26274.354000131717160060 |
ID : | 05-0422331 |
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Pascal:05-0422331Le document en format XML
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<front><div type="abstract" xml:lang="en">To explore the potential of using non-human coronaviruses for cancer therapy, we first established their ability to kill human tumor cells. We found that the feline infectious peritonitis virus (FIPV) and a felinized murine hepatitis virus (fMHV), both normally incapable of infecting human cells, could rapidly and effectively kill human cancer cells artificially expressing the feline coronavirus receptor aminopeptidase N. Also 3-D multilayer tumor spheroids established from such cells were effectively eradicated. Next, we investigated whether FIPV and fMHV could be targeted to human cancer cells by constructing a bispecific single-chain antibody directed on the one hand against the feline coronavirus spike protein - responsible for receptor binding and subsequent cell entry through virus-cell membrane fusion - and on the other hand against the human epidermal growth factor receptor (EGFR). The targeting antibody mediated specific infection of EGFR-expressing human cancer cells by both coronaviruses. Furthermore, in the presence of the targeting antibody, infected cancer cells formed syncytia typical of productive coronavirus infection. By their potent cytotoxicity, the selective targeting of non-human coronaviruses to human cancer cells provides a rationale for further investigations into the use of these viruses as anticancer agents.</div>
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<s5>04</s5>
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<fC03 i1="04" i2="X" l="SPA"><s0>Anticuerpo simple cadena</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Thérapie génique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Gene therapy</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Terapia génica</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Tumeur maligne</s0>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Malignant tumor</s0>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Tumor maligno</s0>
<s5>14</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>16</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>16</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>16</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Récepteur EGFR</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Epidermal growth factor receptor</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Receptor EGFR</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fN21><s1>290</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 05-0422331 INIST</NO>
<ET>Targeting non-human corona viruses to human cancer cells using a bispecific single-chain antibody</ET>
<AU>WÜRDINGER (T.); VERHEIJE (M. H.); RAABEN (M.); BOSCH (B. J.); DE HAAN (C. A. M.); VAN BEUSECHEM (V. W.); ROTTIER (P. J. M.); GERRITSEN (W. R.)</AU>
<AF>Virology Division, Department of Infectious Diseases and Immunology, Utrecht University/Utrecht/Pays-Bas (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 7 aut.); Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center/Amsterdam/Pays-Bas (1 aut., 2 aut., 6 aut., 8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Gene therapy : (Basingstoke); ISSN 0969-7128; Royaume-Uni; Da. 2005; Vol. 12; No. 18; Pp. 1394-1404; Bibl. 42 ref.</SO>
<LA>Anglais</LA>
<EA>To explore the potential of using non-human coronaviruses for cancer therapy, we first established their ability to kill human tumor cells. We found that the feline infectious peritonitis virus (FIPV) and a felinized murine hepatitis virus (fMHV), both normally incapable of infecting human cells, could rapidly and effectively kill human cancer cells artificially expressing the feline coronavirus receptor aminopeptidase N. Also 3-D multilayer tumor spheroids established from such cells were effectively eradicated. Next, we investigated whether FIPV and fMHV could be targeted to human cancer cells by constructing a bispecific single-chain antibody directed on the one hand against the feline coronavirus spike protein - responsible for receptor binding and subsequent cell entry through virus-cell membrane fusion - and on the other hand against the human epidermal growth factor receptor (EGFR). The targeting antibody mediated specific infection of EGFR-expressing human cancer cells by both coronaviruses. Furthermore, in the presence of the targeting antibody, infected cancer cells formed syncytia typical of productive coronavirus infection. By their potent cytotoxicity, the selective targeting of non-human coronaviruses to human cancer cells provides a rationale for further investigations into the use of these viruses as anticancer agents.</EA>
<CC>002A31D01D; 002B27D03; 215</CC>
<FD>Ciblage; Homme; Anticorps bispécifique; Anticorps simple chaîne; Thérapie génique; Tumeur maligne; Coronavirus; Récepteur EGFR</FD>
<FG>Coronaviridae; Nidovirales; Virus</FG>
<ED>Targeting; Human; Bispecific antibody; Single chain antibody; Gene therapy; Malignant tumor; Coronavirus; Epidermal growth factor receptor</ED>
<EG>Coronaviridae; Nidovirales; Virus</EG>
<SD>Blancado; Hombre; Anticuerpo biespecífico; Anticuerpo simple cadena; Terapia génica; Tumor maligno; Coronavirus; Receptor EGFR</SD>
<LO>INIST-26274.354000131717160060</LO>
<ID>05-0422331</ID>
</server>
</inist>
</record>
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