CovidV1, PascalFrancis, Corpus, bibRecord, 000034

DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes

Identifieur interne : 000034 ( PascalFrancis/Corpus ); précédent : 000033; suivant : 000035

DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes

Auteurs : N. Zari ; H. Mohammedi ; A. Amine ; M. M. Ennaji

Source :

Analytical letters [ 0003-2719 ] ; 2007.

RBID : Pascal:07-0341588

Descripteurs français

Pascal (Inist)
- Hydrolyse acide, Voltammétrie, Biodétecteur, Onde carrée, Méthode électrochimique, Oxydation, Electrode carbone, Electrode pâte, Sensibilité, Analyse trace, Température ambiante, Capteur chimique, DNA, Guanine, Adénine, Or.

English descriptors

KwdEn :
- Acid hydrolysis, Adenine, Biosensor, Carbon electrode, Chemical sensor, DNA, Electrochemical method, Gold, Guanine, Oxidation, Paste electrode, Room temperature, Sensitivity, Square wave, Trace analysis, Voltammetry.

Abstract

This work reports the development of a biosensor method for the label-free detection of specific DNA sequences. In the initial phase, square wave voltammetry (SWV) was used in a comparative investigation into the electrochemical oxidation of purines (guanine and adenine) and DNA fragments at various electrode surfaces: carbon paste (CPE), glassy carbon electrode (GCE), and gold (AuE). Relative to the carbon electrodes, an approximate 4.0-fold, 6.0-fold, and 3.25-fold increase in the anodic response was observed when guanine, adenine, and hydrolyzed DNA, respectively, were measured on the AuE. It was shown that the guanine and adenine bases could be successfully determined by use of SWV for a deoxyribonucleic acid sample following acid hydrolysis. This label-free detection of hydrolyzed DNA on gold electrodes has significant advantages over methods using existing carbon electrode materials because of its higher sensitivity and the potential applicability of microfabrication techniques for the production of the requisite gold electrodes. In another phase of development, the times and conditions for DNA hydrolysis and purine release were investigated. It was shown that under optimal conditions, trace levels of the purine bases could be readily detected following 20 min of hydrolysis at room temperature. The proposed method can be used to estimate the guanine and Finally, when appropriate probe sequences were first adsorbed on the surface of the screen-printed gold electrode (SPGE), this electrochemical biosensor could be used to specifically detect sequences from ss corona virus aviair following hybridization and hydrolysis reactions on the sensor surface. No enhancement of the voltammetric response was observed when the sensor was challenged with a non-complementary DNA sequence.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`02`	`1`		`@1 MOHAMMEDI (H.)`
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A14	`01`			`@1 Laboratoire des Analyses Chimiques et Biocapteurs, Faculté des Sciences et Techniques @2 Mohammedia @3 MAR @Z 1 aut. @Z 2 aut. @Z 3 aut.`
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A44				`@0 0000 @1 © 2007 INIST-CNRS. All rights reserved.`
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C01	`01`		`ENG`	@0 This work reports the development of a biosensor method for the label-free detection of specific DNA sequences. In the initial phase, square wave voltammetry (SWV) was used in a comparative investigation into the electrochemical oxidation of purines (guanine and adenine) and DNA fragments at various electrode surfaces: carbon paste (CPE), glassy carbon electrode (GCE), and gold (AuE). Relative to the carbon electrodes, an approximate 4.0-fold, 6.0-fold, and 3.25-fold increase in the anodic response was observed when guanine, adenine, and hydrolyzed DNA, respectively, were measured on the AuE. It was shown that the guanine and adenine bases could be successfully determined by use of SWV for a deoxyribonucleic acid sample following acid hydrolysis. This label-free detection of hydrolyzed DNA on gold electrodes has significant advantages over methods using existing carbon electrode materials because of its higher sensitivity and the potential applicability of microfabrication techniques for the production of the requisite gold electrodes. In another phase of development, the times and conditions for DNA hydrolysis and purine release were investigated. It was shown that under optimal conditions, trace levels of the purine bases could be readily detected following 20 min of hydrolysis at room temperature. The proposed method can be used to estimate the guanine and Finally, when appropriate probe sequences were first adsorbed on the surface of the screen-printed gold electrode (SPGE), this electrochemical biosensor could be used to specifically detect sequences from ss corona virus aviair following hybridization and hydrolysis reactions on the sensor surface. No enhancement of the voltammetric response was observed when the sensor was challenged with a non-complementary DNA sequence.
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N21				`@1 218`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 07-0341588 INIST
ET :	DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes
AU :	ZARI (N.); MOHAMMEDI (H.); AMINE (A.); ENNAJI (M. M.)
AF :	Laboratoire des Analyses Chimiques et Biocapteurs, Faculté des Sciences et Techniques/Mohammedia/Maroc (1 aut., 2 aut., 3 aut.); Laboratoire de Virologie et Hygiène & Microbiologie, Faculté des Sciences et Techniques/Mohammedia/Maroc (4 aut.)
DT :	Publication en série; Niveau analytique
SO :	Analytical letters; ISSN 0003-2719; Coden ANALBP; Etats-Unis; Da. 2007; Vol. 40; No. 7-9; Pp. 1698-1713; Bibl. 1 p.1/2
LA :	Anglais
EA :	This work reports the development of a biosensor method for the label-free detection of specific DNA sequences. In the initial phase, square wave voltammetry (SWV) was used in a comparative investigation into the electrochemical oxidation of purines (guanine and adenine) and DNA fragments at various electrode surfaces: carbon paste (CPE), glassy carbon electrode (GCE), and gold (AuE). Relative to the carbon electrodes, an approximate 4.0-fold, 6.0-fold, and 3.25-fold increase in the anodic response was observed when guanine, adenine, and hydrolyzed DNA, respectively, were measured on the AuE. It was shown that the guanine and adenine bases could be successfully determined by use of SWV for a deoxyribonucleic acid sample following acid hydrolysis. This label-free detection of hydrolyzed DNA on gold electrodes has significant advantages over methods using existing carbon electrode materials because of its higher sensitivity and the potential applicability of microfabrication techniques for the production of the requisite gold electrodes. In another phase of development, the times and conditions for DNA hydrolysis and purine release were investigated. It was shown that under optimal conditions, trace levels of the purine bases could be readily detected following 20 min of hydrolysis at room temperature. The proposed method can be used to estimate the guanine and Finally, when appropriate probe sequences were first adsorbed on the surface of the screen-printed gold electrode (SPGE), this electrochemical biosensor could be used to specifically detect sequences from ss corona virus aviair following hybridization and hydrolysis reactions on the sensor surface. No enhancement of the voltammetric response was observed when the sensor was challenged with a non-complementary DNA sequence.
CC :	001C04E; 002A31C09B; 001C04A; 215
FD :	Hydrolyse acide; Voltammétrie; Biodétecteur; Onde carrée; Méthode électrochimique; Oxydation; Electrode carbone; Electrode pâte; Sensibilité; Analyse trace; Température ambiante; Capteur chimique; DNA; Guanine; Adénine; Or
ED :	Acid hydrolysis; Voltammetry; Biosensor; Square wave; Electrochemical method; Oxidation; Carbon electrode; Paste electrode; Sensitivity; Trace analysis; Room temperature; Chemical sensor; DNA; Guanine; Adenine; Gold
SD :	Voltametría; Biodetector; Onda cuadrada; Método electroquímico; Oxidación; Electrodo carbono; Electrodo pasta; Sensibilidad; Análisis huella; Temperatura ambiente; Captador químico; DNA; Guanina; Adenina; Oro
LO :	INIST-13703.354000162914610330
ID :	07-0341588

Links to Exploration step

Pascal:07-0341588

Le document en format XML

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<front><div type="abstract" xml:lang="en">This work reports the development of a biosensor method for the label-free detection of specific DNA sequences. In the initial phase, square wave voltammetry (SWV) was used in a comparative investigation into the electrochemical oxidation of purines (guanine and adenine) and DNA fragments at various electrode surfaces: carbon paste (CPE), glassy carbon electrode (GCE), and gold (AuE). Relative to the carbon electrodes, an approximate 4.0-fold, 6.0-fold, and 3.25-fold increase in the anodic response was observed when guanine, adenine, and hydrolyzed DNA, respectively, were measured on the AuE. It was shown that the guanine and adenine bases could be successfully determined by use of SWV for a deoxyribonucleic acid sample following acid hydrolysis. This label-free detection of hydrolyzed DNA on gold electrodes has significant advantages over methods using existing carbon electrode materials because of its higher sensitivity and the potential applicability of microfabrication techniques for the production of the requisite gold electrodes. In another phase of development, the times and conditions for DNA hydrolysis and purine release were investigated. It was shown that under optimal conditions, trace levels of the purine bases could be readily detected following 20 min of hydrolysis at room temperature. The proposed method can be used to estimate the guanine and  Finally, when appropriate probe sequences were first adsorbed on the surface of the screen-printed gold electrode (SPGE), this electrochemical biosensor could be used to specifically detect sequences from ss corona virus aviair following hybridization and hydrolysis reactions on the sensor surface. No enhancement of the voltammetric response was observed when the sensor was challenged with a non-complementary DNA sequence.</div>
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</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Biodétecteur</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Biosensor</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Biodetector</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Onde carrée</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Square wave</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Onda cuadrada</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Méthode électrochimique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Electrochemical method</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Método electroquímico</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Oxydation</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Oxidation</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Oxidación</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Electrode carbone</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Carbon electrode</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Electrodo carbono</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Electrode pâte</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Paste electrode</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Electrodo pasta</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Sensibilité</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Sensitivity</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Sensibilidad</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Analyse trace</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Trace analysis</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Análisis huella</s0>
<s5>10</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Température ambiante</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Room temperature</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Temperatura ambiente</s0>
<s5>11</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Capteur chimique</s0>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Chemical sensor</s0>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Captador químico</s0>
<s5>12</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>DNA</s0>
<s5>15</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>DNA</s0>
<s5>15</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>DNA</s0>
<s5>15</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Guanine</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Guanine</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Guanina</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Adénine</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>17</s5>
</fC03>
<fC03 i1="15" i2="X" l="ENG"><s0>Adenine</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>17</s5>
</fC03>
<fC03 i1="15" i2="X" l="SPA"><s0>Adenina</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>17</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Or</s0>
<s2>NC</s2>
<s5>18</s5>
</fC03>
<fC03 i1="16" i2="X" l="ENG"><s0>Gold</s0>
<s2>NC</s2>
<s5>18</s5>
</fC03>
<fC03 i1="16" i2="X" l="SPA"><s0>Oro</s0>
<s2>NC</s2>
<s5>18</s5>
</fC03>
<fN21><s1>218</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 07-0341588 INIST</NO>
<ET>DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes</ET>
<AU>ZARI (N.); MOHAMMEDI (H.); AMINE (A.); ENNAJI (M. M.)</AU>
<AF>Laboratoire des Analyses Chimiques et Biocapteurs, Faculté des Sciences et Techniques/Mohammedia/Maroc (1 aut., 2 aut., 3 aut.); Laboratoire de Virologie et Hygiène & Microbiologie, Faculté des Sciences et Techniques/Mohammedia/Maroc (4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Analytical letters; ISSN 0003-2719; Coden ANALBP; Etats-Unis; Da. 2007; Vol. 40; No. 7-9; Pp. 1698-1713; Bibl. 1 p.1/2</SO>
<LA>Anglais</LA>
<EA>This work reports the development of a biosensor method for the label-free detection of specific DNA sequences. In the initial phase, square wave voltammetry (SWV) was used in a comparative investigation into the electrochemical oxidation of purines (guanine and adenine) and DNA fragments at various electrode surfaces: carbon paste (CPE), glassy carbon electrode (GCE), and gold (AuE). Relative to the carbon electrodes, an approximate 4.0-fold, 6.0-fold, and 3.25-fold increase in the anodic response was observed when guanine, adenine, and hydrolyzed DNA, respectively, were measured on the AuE. It was shown that the guanine and adenine bases could be successfully determined by use of SWV for a deoxyribonucleic acid sample following acid hydrolysis. This label-free detection of hydrolyzed DNA on gold electrodes has significant advantages over methods using existing carbon electrode materials because of its higher sensitivity and the potential applicability of microfabrication techniques for the production of the requisite gold electrodes. In another phase of development, the times and conditions for DNA hydrolysis and purine release were investigated. It was shown that under optimal conditions, trace levels of the purine bases could be readily detected following 20 min of hydrolysis at room temperature. The proposed method can be used to estimate the guanine and  Finally, when appropriate probe sequences were first adsorbed on the surface of the screen-printed gold electrode (SPGE), this electrochemical biosensor could be used to specifically detect sequences from ss corona virus aviair following hybridization and hydrolysis reactions on the sensor surface. No enhancement of the voltammetric response was observed when the sensor was challenged with a non-complementary DNA sequence.</EA>
<CC>001C04E; 002A31C09B; 001C04A; 215</CC>
<FD>Hydrolyse acide; Voltammétrie; Biodétecteur; Onde carrée; Méthode électrochimique; Oxydation; Electrode carbone; Electrode pâte; Sensibilité; Analyse trace; Température ambiante; Capteur chimique; DNA; Guanine; Adénine; Or</FD>
<ED>Acid hydrolysis; Voltammetry; Biosensor; Square wave; Electrochemical method; Oxidation; Carbon electrode; Paste electrode; Sensitivity; Trace analysis; Room temperature; Chemical sensor; DNA; Guanine; Adenine; Gold</ED>
<SD>Voltametría; Biodetector; Onda cuadrada; Método electroquímico; Oxidación; Electrodo carbono; Electrodo pasta; Sensibilidad; Análisis huella; Temperatura ambiente; Captador químico; DNA; Guanina; Adenina; Oro</SD>
<LO>INIST-13703.354000162914610330</LO>
<ID>07-0341588</ID>
</server>
</inist>
</record>

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DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes

DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes

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