A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona Virus.
Identifieur interne : 000557 ( Ncbi/Merge ); précédent : 000556; suivant : 000558A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona Virus.
Auteurs : Il Young Jung [Corée du Sud] ; Jae Bem You [Corée du Sud] ; Bo Ram Choi [Corée du Sud] ; Ji Su Kim [Corée du Sud] ; Hyun Kyung Lee [Corée du Sud] ; Bora Jang [Corée du Sud] ; Han Saem Jeong [Corée du Sud] ; Kyuri Lee [Corée du Sud] ; Sung Gap Im [Corée du Sud] ; Hyukjin Lee [Corée du Sud]Source :
- Advanced healthcare materials [ 2192-2659 ] ; 2016.
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Abstract
Trail polymerization enables a significant enhancement of the DhITACT system. DhITACT-Trail (DNA hydrogel formation by isothermal amplification of complementary targets trail polymerization) offers a robust diagnosis of target RNA strands in pseudo-serum specimen. This system requires minimum liquid handling as compared to conventional analysis. In addition, a definitive diagnostic result can be achieved within 30 min by an optical detection.
DOI: 10.1002/adhm.201600334
PubMed: 27332622
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Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750</wicri:regionArea><wicri:noRegion>120-750</wicri:noRegion></affiliation></author><author><name sortKey="Jeong, Han Saem" sort="Jeong, Han Saem" uniqKey="Jeong H" first="Han Saem" last="Jeong">Han Saem Jeong</name><affiliation wicri:level="1"><nlm:affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750</wicri:regionArea><wicri:noRegion>120-750</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Kyuri" sort="Lee, Kyuri" uniqKey="Lee K" first="Kyuri" last="Lee">Kyuri Lee</name><affiliation wicri:level="1"><nlm:affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750</wicri:regionArea><wicri:noRegion>120-750</wicri:noRegion></affiliation></author><author><name sortKey="Im, Sung Gap" sort="Im, Sung Gap" uniqKey="Im S" first="Sung Gap" last="Im">Sung Gap Im</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea. sgim@kaist.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141</wicri:regionArea><wicri:noRegion>34141</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Hyukjin" sort="Lee, Hyukjin" uniqKey="Lee H" first="Hyukjin" last="Lee">Hyukjin Lee</name><affiliation wicri:level="1"><nlm:affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea. hyukjin@ewha.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750</wicri:regionArea><wicri:noRegion>120-750</wicri:noRegion></affiliation></author></analytic><series><title level="j">Advanced healthcare materials</title><idno type="eISSN">2192-2659</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques</term><term>Coronavirus Infections (diagnosis)</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Sensibilité et spécificité</term><term>Techniques de biocapteur</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biosensing Techniques</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Humains</term><term>Sensibilité et spécificité</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Trail polymerization enables a significant enhancement of the DhITACT system. DhITACT-Trail (DNA hydrogel formation by isothermal amplification of complementary targets trail polymerization) offers a robust diagnosis of target RNA strands in pseudo-serum specimen. This system requires minimum liquid handling as compared to conventional analysis. In addition, a definitive diagnostic result can be achieved within 30 min by an optical detection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27332622</PMID><DateCompleted><Year>2017</Year><Month>11</Month><Day>29</Day></DateCompleted><DateRevised><Year>2018</Year><Month>08</Month><Day>31</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2192-2659</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><Issue>17</Issue><PubDate><Year>2016</Year><Month>09</Month></PubDate></JournalIssue><Title>Advanced healthcare materials</Title><ISOAbbreviation>Adv Healthc Mater</ISOAbbreviation></Journal><ArticleTitle>A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona Virus.</ArticleTitle><Pagination><MedlinePgn>2168-73</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/adhm.201600334</ELocationID><Abstract><AbstractText>Trail polymerization enables a significant enhancement of the DhITACT system. DhITACT-Trail (DNA hydrogel formation by isothermal amplification of complementary targets trail polymerization) offers a robust diagnosis of target RNA strands in pseudo-serum specimen. This system requires minimum liquid handling as compared to conventional analysis. In addition, a definitive diagnostic result can be achieved within 30 min by an optical detection.</AbstractText><CopyrightInformation>© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jung</LastName><ForeName>Il Young</ForeName><Initials>IY</Initials><AffiliationInfo><Affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>You</LastName><ForeName>Jae Bem</ForeName><Initials>JB</Initials><AffiliationInfo><Affiliation>Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Bo Ram</ForeName><Initials>BR</Initials><AffiliationInfo><Affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Ji Su</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Hyun Kyung</ForeName><Initials>HK</Initials><AffiliationInfo><Affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jang</LastName><ForeName>Bora</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jeong</LastName><ForeName>Han Saem</ForeName><Initials>HS</Initials><AffiliationInfo><Affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Kyuri</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Im</LastName><ForeName>Sung Gap</ForeName><Initials>SG</Initials><AffiliationInfo><Affiliation>Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea. sgim@kaist.ac.kr.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>KAIST Institute for Nano Century, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea. sgim@kaist.ac.kr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Hyukjin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 120-750, Republic of Korea. hyukjin@ewha.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>06</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Adv Healthc Mater</MedlineTA><NlmUniqueID>101581613</NlmUniqueID><ISSNLinking>2192-2640</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015374" MajorTopicYN="Y">Biosensing Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="Y">Middle East Respiratory Syndrome Coronavirus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>03</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2006</Year><Month>04</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>6</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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last="Jang">Bora Jang</name><name sortKey="Jeong, Han Saem" sort="Jeong, Han Saem" uniqKey="Jeong H" first="Han Saem" last="Jeong">Han Saem Jeong</name><name sortKey="Kim, Ji Su" sort="Kim, Ji Su" uniqKey="Kim J" first="Ji Su" last="Kim">Ji Su Kim</name><name sortKey="Lee, Hyukjin" sort="Lee, Hyukjin" uniqKey="Lee H" first="Hyukjin" last="Lee">Hyukjin Lee</name><name sortKey="Lee, Hyun Kyung" sort="Lee, Hyun Kyung" uniqKey="Lee H" first="Hyun Kyung" last="Lee">Hyun Kyung Lee</name><name sortKey="Lee, Kyuri" sort="Lee, Kyuri" uniqKey="Lee K" first="Kyuri" last="Lee">Kyuri Lee</name><name sortKey="You, Jae Bem" sort="You, Jae Bem" uniqKey="You J" first="Jae Bem" last="You">Jae Bem You</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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