Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification
Identifieur interne : 000540 ( Ncbi/Merge ); précédent : 000539; suivant : 000541Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification
Auteurs : B. Radhika [Inde] ; N. Vinod Kumar [Inde] ; D. Sreenivasulu [Inde]Source :
- Veterinary World [ 0972-8988 ] ; 2016.
Abstract
The loop mediated isothermal amplification (LAMP) was standardized for rapid detection of
A total of 120 fecal samples were collected from enterotoxemia suspected lambs were used for screening of
Out of 120 samples screened 112 (93.3%) samples were positive by both LAMP and polymerase chain reaction (PCR) for detection of
Both LAMP and PCR for detection of
Url:
DOI: 10.14202/vetworld.2016.60-64
PubMed: 27051186
PubMed Central: 4819352
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000049
- to stream Pmc, to step Curation: 000049
- to stream Pmc, to step Checkpoint: 000279
Links to Exploration step
PMC:4819352Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of <italic>Clostridium perfringens</italic>
alpha toxin gene in lambs by loop mediated isothermal amplification</title>
<author><name sortKey="Radhika, B" sort="Radhika, B" uniqKey="Radhika B" first="B." last="Radhika">B. Radhika</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">State Level Diagnostic Laboratory, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea>State Level Diagnostic Laboratory, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea>
<wicri:noRegion>Andhra Pradesh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kumar, N Vinod" sort="Kumar, N Vinod" uniqKey="Kumar N" first="N. Vinod" last="Kumar">N. Vinod Kumar</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea>Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea>
<wicri:noRegion>Andhra Pradesh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sreenivasulu, D" sort="Sreenivasulu, D" uniqKey="Sreenivasulu D" first="D." last="Sreenivasulu">D. Sreenivasulu</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea>Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea>
<wicri:noRegion>Andhra Pradesh</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27051186</idno>
<idno type="pmc">4819352</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819352</idno>
<idno type="RBID">PMC:4819352</idno>
<idno type="doi">10.14202/vetworld.2016.60-64</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">000049</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000049</idno>
<idno type="wicri:Area/Pmc/Curation">000049</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000049</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000279</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000279</idno>
<idno type="wicri:Area/Ncbi/Merge">000540</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Detection of <italic>Clostridium perfringens</italic>
alpha toxin gene in lambs by loop mediated isothermal amplification</title>
<author><name sortKey="Radhika, B" sort="Radhika, B" uniqKey="Radhika B" first="B." last="Radhika">B. Radhika</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">State Level Diagnostic Laboratory, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea>State Level Diagnostic Laboratory, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea>
<wicri:noRegion>Andhra Pradesh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kumar, N Vinod" sort="Kumar, N Vinod" uniqKey="Kumar N" first="N. Vinod" last="Kumar">N. Vinod Kumar</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea>Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea>
<wicri:noRegion>Andhra Pradesh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sreenivasulu, D" sort="Sreenivasulu, D" uniqKey="Sreenivasulu D" first="D." last="Sreenivasulu">D. Sreenivasulu</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff>
<country xml:lang="fr">Inde</country>
<wicri:regionArea>Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea>
<wicri:noRegion>Andhra Pradesh</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Veterinary World</title>
<idno type="ISSN">0972-8988</idno>
<idno type="eISSN">2231-0916</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec id="st1"><title>Aim:</title>
<p>The loop mediated isothermal amplification (LAMP) was standardized for rapid detection of <italic>Clostridium perfringens</italic>
.</p>
</sec>
<sec id="st2"><title>Materials and Methods:</title>
<p>A total of 120 fecal samples were collected from enterotoxemia suspected lambs were used for screening of <italic>C. perfringens cpa</italic>
gene by LAMP. The specificity of the LAMP amplified products was tested by digesting with restriction enzyme <italic>XmnI</italic>
for alpha toxin gene.</p>
</sec>
<sec id="st3"><title>Results:</title>
<p>Out of 120 samples screened 112 (93.3%) samples were positive by both LAMP and polymerase chain reaction (PCR) for detection of <italic>cpa</italic>
gene which indicated the equal sensitivity of both the tests. The enzyme produced single cut in 162 base pair amplified product of alpha toxin gene at 81 base pair resulting in a single band in gel electrophoresis.</p>
</sec>
<sec id="st4"><title>Conclusion:</title>
<p>Both LAMP and PCR for detection of <italic>cpa</italic>
gene indicated the equal sensitivity of both the tests. Standardization of LAMP reaction for amplification of epsilon and beta toxin genes will help to identify the <italic>C. perfringens</italic>
toxin types from the clinical samples. The test could be a suitable alternative to the PCR in detection of toxin types without the help of sophisticated machinery like thermal cycler. Considering its simplicity in operation and high sensitivity, there is the potential use of this technique in clinical diagnosis and surveillance of infectious diseases.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Uzal, F A" uniqKey="Uzal F">F.A Uzal</name>
</author>
<author><name sortKey="Plumb, J" uniqKey="Plumb J">J Plumb</name>
</author>
<author><name sortKey="Blackall, L L" uniqKey="Blackall L">L.L Blackall</name>
</author>
<author><name sortKey="O Oyle, D" uniqKey="O Oyle D">D O’Boyle</name>
</author>
<author><name sortKey="Kelly, W R" uniqKey="Kelly W">W.R Kelly</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dray, T" uniqKey="Dray T">T Dray</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Khairy, E M" uniqKey="Khairy E">E.M Khairy</name>
</author>
<author><name sortKey="Dorgham, S M" uniqKey="Dorgham S">S.M Dorgham</name>
</author>
<author><name sortKey="Bakry, M A" uniqKey="Bakry M">M.A Bakry</name>
</author>
<author><name sortKey="Hakim, A S" uniqKey="Hakim A">A.S Hakim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meer, R R" uniqKey="Meer R">R.R Meer</name>
</author>
<author><name sortKey="Songer, J G" uniqKey="Songer J">J.G Songer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vinod Kumar, N" uniqKey="Vinod Kumar N">N Vinod Kumar</name>
</author>
<author><name sortKey="Sreenivasulu, D" uniqKey="Sreenivasulu D">D Sreenivasulu</name>
</author>
<author><name sortKey="Reddy, Y N" uniqKey="Reddy Y">Y.N Reddy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Parida, M" uniqKey="Parida M">M Parida</name>
</author>
<author><name sortKey="Sannarangaiah, S" uniqKey="Sannarangaiah S">S Sannarangaiah</name>
</author>
<author><name sortKey="Dash, P K" uniqKey="Dash P">P.K Dash</name>
</author>
<author><name sortKey="Rao, P V L" uniqKey="Rao P">P.V.L Rao</name>
</author>
<author><name sortKey="Morita, K" uniqKey="Morita K">K Morita</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name>
</author>
<author><name sortKey="Okayama, H" uniqKey="Okayama H">H Okayama</name>
</author>
<author><name sortKey="Masubuchi, H" uniqKey="Masubuchi H">H Masubuchi</name>
</author>
<author><name sortKey="Yonekawa, T" uniqKey="Yonekawa T">T Yonekawa</name>
</author>
<author><name sortKey="Watanabe, K" uniqKey="Watanabe K">K Watanabe</name>
</author>
<author><name sortKey="Amino, N" uniqKey="Amino N">N Amino</name>
</author>
<author><name sortKey="Hase, T" uniqKey="Hase T">T Hase</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mori, Y" uniqKey="Mori Y">Y Mori</name>
</author>
<author><name sortKey="Nagamine, K" uniqKey="Nagamine K">K Nagamine</name>
</author>
<author><name sortKey="Tomita, N" uniqKey="Tomita N">N Tomita</name>
</author>
<author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nagamine, K" uniqKey="Nagamine K">K Nagamine</name>
</author>
<author><name sortKey="Watanabe, K" uniqKey="Watanabe K">K Watanabe</name>
</author>
<author><name sortKey="Ohtsuka, K" uniqKey="Ohtsuka K">K Ohtsuka</name>
</author>
<author><name sortKey="Hase, T" uniqKey="Hase T">T Hase</name>
</author>
<author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nagamine, K" uniqKey="Nagamine K">K Nagamine</name>
</author>
<author><name sortKey="Hase, T" uniqKey="Hase T">T Hase</name>
</author>
<author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sambrook, J" uniqKey="Sambrook J">J Sambrook</name>
</author>
<author><name sortKey="Russel, D W" uniqKey="Russel D">D.W Russel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kamachi, K" uniqKey="Kamachi K">K Kamachi</name>
</author>
<author><name sortKey="Toyoizumi Ajisaka, H" uniqKey="Toyoizumi Ajisaka H">H Toyoizumi-Ajisaka</name>
</author>
<author><name sortKey="Toda, K" uniqKey="Toda K">K Toda</name>
</author>
<author><name sortKey="Soeung, S C" uniqKey="Soeung S">S.C Soeung</name>
</author>
<author><name sortKey="Sarath, S" uniqKey="Sarath S">S Sarath</name>
</author>
<author><name sortKey="Nareth, Y" uniqKey="Nareth Y">Y Nareth</name>
</author>
<author><name sortKey="Horiuchi, Y" uniqKey="Horiuchi Y">Y Horiuchi</name>
</author>
<author><name sortKey="Kojima, K" uniqKey="Kojima K">K Kojima</name>
</author>
<author><name sortKey="Takahashi, M" uniqKey="Takahashi M">M Takahashi</name>
</author>
<author><name sortKey="Arakawa, Y" uniqKey="Arakawa Y">Y Arakawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Das, A" uniqKey="Das A">A Das</name>
</author>
<author><name sortKey="Babiuk, S" uniqKey="Babiuk S">S Babiuk</name>
</author>
<author><name sortKey="Mclntosh, M T" uniqKey="Mclntosh M">M.T Mclntosh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kaneko, K" uniqKey="Kaneko K">K Kaneko</name>
</author>
<author><name sortKey="Miyamoto, K" uniqKey="Miyamoto K">K Miyamoto</name>
</author>
<author><name sortKey="Mimura, K" uniqKey="Mimura K">K Mimura</name>
</author>
<author><name sortKey="Yumine, N" uniqKey="Yumine N">N Yumine</name>
</author>
<author><name sortKey="Utsunomiya, H" uniqKey="Utsunomiya H">H Utsunomiya</name>
</author>
<author><name sortKey="Akimoto, S" uniqKey="Akimoto S">S Akimoto</name>
</author>
<author><name sortKey="Mcclane, A B" uniqKey="Mcclane A">A.B McClane</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Aryan, E" uniqKey="Aryan E">E Aryan</name>
</author>
<author><name sortKey="Makvandi, M" uniqKey="Makvandi M">M Makvandi</name>
</author>
<author><name sortKey="Farajzadeh, A" uniqKey="Farajzadeh A">A Farajzadeh</name>
</author>
<author><name sortKey="Huygen, K" uniqKey="Huygen K">K Huygen</name>
</author>
<author><name sortKey="Bifani, P" uniqKey="Bifani P">P Bifani</name>
</author>
<author><name sortKey="Mousavi, S" uniqKey="Mousavi S">S Mousavi</name>
</author>
<author><name sortKey="Fateh, A" uniqKey="Fateh A">A Fateh</name>
</author>
<author><name sortKey="Jelodar, A" uniqKey="Jelodar A">A Jelodar</name>
</author>
<author><name sortKey="Gouya, M" uniqKey="Gouya M">M Gouya</name>
</author>
<author><name sortKey="Romano, M" uniqKey="Romano M">M Romano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Suzuki, R" uniqKey="Suzuki R">R Suzuki</name>
</author>
<author><name sortKey="Ihira, M" uniqKey="Ihira M">M Ihira</name>
</author>
<author><name sortKey="Enomoto, Y" uniqKey="Enomoto Y">Y Enomoto</name>
</author>
<author><name sortKey="Yano, H" uniqKey="Yano H">H Yano</name>
</author>
<author><name sortKey="Maruyama, F" uniqKey="Maruyama F">F Maruyama</name>
</author>
<author><name sortKey="Emi, N" uniqKey="Emi N">N Emi</name>
</author>
<author><name sortKey="Asano, Y" uniqKey="Asano Y">Y Asano</name>
</author>
<author><name sortKey="Yoshikawa, T" uniqKey="Yoshikawa T">T Yoshikawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hong, T C" uniqKey="Hong T">T.C Hong</name>
</author>
<author><name sortKey="Mai, Q L" uniqKey="Mai Q">Q.L Mai</name>
</author>
<author><name sortKey="Cuong, D V" uniqKey="Cuong D">D.V Cuong</name>
</author>
<author><name sortKey="Parida, M" uniqKey="Parida M">M Parida</name>
</author>
<author><name sortKey="Minekawa, H" uniqKey="Minekawa H">H Minekawa</name>
</author>
<author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name>
</author>
<author><name sortKey="Hasebe, F" uniqKey="Hasebe F">F Hasebe</name>
</author>
<author><name sortKey="Morita, K" uniqKey="Morita K">K Morita</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hara Kudo, Y" uniqKey="Hara Kudo Y">Y Hara Kudo</name>
</author>
<author><name sortKey="Yoshino, M" uniqKey="Yoshino M">M Yoshino</name>
</author>
<author><name sortKey="Kojima, T" uniqKey="Kojima T">T Kojima</name>
</author>
<author><name sortKey="Ikedo, M" uniqKey="Ikedo M">M Ikedo</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Vet World</journal-id>
<journal-id journal-id-type="iso-abbrev">Vet World</journal-id>
<journal-id journal-id-type="publisher-id">Vet World</journal-id>
<journal-title-group><journal-title>Veterinary World</journal-title>
</journal-title-group>
<issn pub-type="ppub">0972-8988</issn>
<issn pub-type="epub">2231-0916</issn>
<publisher><publisher-name>Veterinary World</publisher-name>
<publisher-loc>India</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">27051186</article-id>
<article-id pub-id-type="pmc">4819352</article-id>
<article-id pub-id-type="doi">10.14202/vetworld.2016.60-64</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Detection of <italic>Clostridium perfringens</italic>
alpha toxin gene in lambs by loop mediated isothermal amplification</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Radhika</surname>
<given-names>B.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="corresp" rid="cor1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kumar</surname>
<given-names>N. Vinod</given-names>
</name>
<xref ref-type="aff" rid="aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sreenivasulu</surname>
<given-names>D.</given-names>
</name>
<xref ref-type="aff" rid="aff2">2</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
State Level Diagnostic Laboratory, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</aff>
<aff id="aff2"><label>2</label>
Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</aff>
<author-notes><corresp id="cor1"><bold>Corresponding author:</bold>
B. Radhika, e-mail: <email xlink:href="radhi.radhi.71@gmail.com">radhi.radhi.71@gmail.com</email>
, NVK: <email xlink:href="nagaram_vinod@yahoo.com">nagaram_vinod@yahoo.com</email>
, DS: <email xlink:href="dsreenivasulu10@gmail.com">dsreenivasulu10@gmail.com</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>1</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub"><day>20</day>
<month>1</month>
<year>2016</year>
</pub-date>
<volume>9</volume>
<issue>1</issue>
<fpage>60</fpage>
<lpage>64</lpage>
<history><date date-type="received"><day>20</day>
<month>8</month>
<year>2015</year>
</date>
<date date-type="rev-recd"><day>02</day>
<month>12</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>11</day>
<month>12</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>Copyright: © Radhika <italic>et al</italic>
.</copyright-statement>
<copyright-year>2016</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0"><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>
Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>
) applies to the data made available in this article, unless otherwise stated.</license-p>
</license>
</permissions>
<abstract><sec id="st1"><title>Aim:</title>
<p>The loop mediated isothermal amplification (LAMP) was standardized for rapid detection of <italic>Clostridium perfringens</italic>
.</p>
</sec>
<sec id="st2"><title>Materials and Methods:</title>
<p>A total of 120 fecal samples were collected from enterotoxemia suspected lambs were used for screening of <italic>C. perfringens cpa</italic>
gene by LAMP. The specificity of the LAMP amplified products was tested by digesting with restriction enzyme <italic>XmnI</italic>
for alpha toxin gene.</p>
</sec>
<sec id="st3"><title>Results:</title>
<p>Out of 120 samples screened 112 (93.3%) samples were positive by both LAMP and polymerase chain reaction (PCR) for detection of <italic>cpa</italic>
gene which indicated the equal sensitivity of both the tests. The enzyme produced single cut in 162 base pair amplified product of alpha toxin gene at 81 base pair resulting in a single band in gel electrophoresis.</p>
</sec>
<sec id="st4"><title>Conclusion:</title>
<p>Both LAMP and PCR for detection of <italic>cpa</italic>
gene indicated the equal sensitivity of both the tests. Standardization of LAMP reaction for amplification of epsilon and beta toxin genes will help to identify the <italic>C. perfringens</italic>
toxin types from the clinical samples. The test could be a suitable alternative to the PCR in detection of toxin types without the help of sophisticated machinery like thermal cycler. Considering its simplicity in operation and high sensitivity, there is the potential use of this technique in clinical diagnosis and surveillance of infectious diseases.</p>
</sec>
</abstract>
<kwd-group><kwd><italic>Clostridium perfringens</italic>
</kwd>
<kwd>enterotoxemia</kwd>
<kwd>lambs</kwd>
<kwd>loop mediated isothermal amplification</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Inde</li>
</country>
</list>
<tree><country name="Inde"><noRegion><name sortKey="Radhika, B" sort="Radhika, B" uniqKey="Radhika B" first="B." last="Radhika">B. Radhika</name>
</noRegion>
<name sortKey="Kumar, N Vinod" sort="Kumar, N Vinod" uniqKey="Kumar N" first="N. Vinod" last="Kumar">N. Vinod Kumar</name>
<name sortKey="Sreenivasulu, D" sort="Sreenivasulu, D" uniqKey="Sreenivasulu D" first="D." last="Sreenivasulu">D. Sreenivasulu</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000540 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000540 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV1 |flux= Ncbi |étape= Merge |type= RBID |clé= PMC:4819352 |texte= Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:27051186" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a CovidV1
This area was generated with Dilib version V0.6.33. |