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Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus

Identifieur interne : 000147 ( Ncbi/Merge ); précédent : 000146; suivant : 000148

Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus

Auteurs : Anqi Yang [République populaire de Chine] ; Lei Wei [République populaire de Chine] ; Weiran Zhao [République populaire de Chine] ; Yuanyuan Xu [République populaire de Chine] ; Zihe Rao [République populaire de Chine]

Source :

RBID : PMC:2720334

Abstract

The N-terminal domain of nsp2 from avian infectious bronchitis virus has been purified and crystallized. The crystals diffracted to 2.5 Å resolution.


Url:
DOI: 10.1107/S1744309109024749
PubMed: 19652340
PubMed Central: 2720334

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PMC:2720334

Le document en format XML

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<name sortKey="Yang, Anqi" sort="Yang, Anqi" uniqKey="Yang A" first="Anqi" last="Yang">Anqi Yang</name>
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<nlm:aff id="a">Tsinghua–Nankai–IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084,
<country>People’s Republic of China</country>
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<country xml:lang="fr">République populaire de Chine</country>
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<name sortKey="Wei, Lei" sort="Wei, Lei" uniqKey="Wei L" first="Lei" last="Wei">Lei Wei</name>
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<name sortKey="Zhao, Weiran" sort="Zhao, Weiran" uniqKey="Zhao W" first="Weiran" last="Zhao">Weiran Zhao</name>
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<name sortKey="Xu, Yuanyuan" sort="Xu, Yuanyuan" uniqKey="Xu Y" first="Yuanyuan" last="Xu">Yuanyuan Xu</name>
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<title xml:lang="en" level="a" type="main">Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus</title>
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<name sortKey="Yang, Anqi" sort="Yang, Anqi" uniqKey="Yang A" first="Anqi" last="Yang">Anqi Yang</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<author>
<name sortKey="Wei, Lei" sort="Wei, Lei" uniqKey="Wei L" first="Lei" last="Wei">Lei Wei</name>
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<nlm:aff id="a">Tsinghua–Nankai–IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084,
<country>People’s Republic of China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author>
<name sortKey="Zhao, Weiran" sort="Zhao, Weiran" uniqKey="Zhao W" first="Weiran" last="Zhao">Weiran Zhao</name>
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<nlm:aff id="a">Tsinghua–Nankai–IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084,
<country>People’s Republic of China</country>
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<country xml:lang="fr">République populaire de Chine</country>
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<name sortKey="Xu, Yuanyuan" sort="Xu, Yuanyuan" uniqKey="Xu Y" first="Yuanyuan" last="Xu">Yuanyuan Xu</name>
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<nlm:aff id="a">Tsinghua–Nankai–IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084,
<country>People’s Republic of China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
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<name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name>
<affiliation wicri:level="1">
<nlm:aff id="a">Tsinghua–Nankai–IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084,
<country>People’s Republic of China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<title level="j">Acta Crystallographica Section F: Structural Biology and Crystallization Communications</title>
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<p>The N-terminal domain of nsp2 from avian infectious bronchitis virus has been purified and crystallized. The crystals diffracted to 2.5 Å resolution.</p>
</div>
</front>
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<journal-title>Acta Crystallographica Section F: Structural Biology and Crystallization Communications</journal-title>
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<publisher-name>International Union of Crystallography</publisher-name>
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<article-id pub-id-type="pmid">19652340</article-id>
<article-id pub-id-type="pmc">2720334</article-id>
<article-id pub-id-type="publisher-id">fw5224</article-id>
<article-id pub-id-type="doi">10.1107/S1744309109024749</article-id>
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<subject>Crystallization Communications</subject>
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<title-group>
<article-title>Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus</article-title>
<alt-title>N-terminal domain of nsp2 from avian infectious bronchitis virus</alt-title>
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<contrib contrib-type="author">
<name>
<surname>Yang</surname>
<given-names>Anqi</given-names>
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<contrib contrib-type="author">
<name>
<surname>Wei</surname>
<given-names>Lei</given-names>
</name>
<xref ref-type="aff" rid="a">a</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Zhao</surname>
<given-names>Weiran</given-names>
</name>
<xref ref-type="aff" rid="a">a</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Xu</surname>
<given-names>Yuanyuan</given-names>
</name>
<xref ref-type="aff" rid="a">a</xref>
<xref ref-type="corresp" rid="cor">*</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Rao</surname>
<given-names>Zihe</given-names>
</name>
<xref ref-type="aff" rid="a">a</xref>
</contrib>
<aff id="a">
<label>a</label>
Tsinghua–Nankai–IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084,
<country>People’s Republic of China</country>
</aff>
</contrib-group>
<author-notes>
<corresp id="cor">Correspondence e-mail:
<email>xuyy@mail.xtal.tsinghua.edu.cn </email>
</corresp>
</author-notes>
<pub-date pub-type="collection">
<day>1</day>
<month>8</month>
<year>2009</year>
</pub-date>
<pub-date pub-type="epub">
<day>25</day>
<month>7</month>
<year>2009</year>
</pub-date>
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<day>1</day>
<month>8</month>
<year>2011</year>
</pub-date>
<pmc-comment> PMC Release delay is 24 months and 0 days and was based on the . </pmc-comment>
<volume>65</volume>
<issue>Pt 8</issue>
<issue-id pub-id-type="publisher-id">f090800</issue-id>
<fpage>788</fpage>
<lpage>790</lpage>
<history>
<date date-type="received">
<day>21</day>
<month>5</month>
<year>2009</year>
</date>
<date date-type="accepted">
<day>26</day>
<month>6</month>
<year>2009</year>
</date>
</history>
<permissions>
<copyright-statement>© International Union of Crystallography 2009</copyright-statement>
<copyright-year>2009</copyright-year>
</permissions>
<self-uri xlink:type="simple" xlink:href="http://dx.doi.org/10.1107/S1744309109024749">A full version of this article is available from Crystallography Journals Online.</self-uri>
<abstract abstract-type="toc">
<p>The N-terminal domain of nsp2 from avian infectious bronchitis virus has been purified and crystallized. The crystals diffracted to 2.5 Å resolution.</p>
</abstract>
<abstract>
<p>Avian infectious bronchitis virus (IBV) is a prototype of the group III coronaviruses and encodes 15 nonstructural proteins which make up the transcription/replication machinery. The nsp2 protein from IBV has a unique and novel sequence and has no experimentally confirmed function in replication, whereas it has been proposed to be crucial for early viral infection and may inhibit the early host immune response. The gene that encodes a double-mutant IBV nsp2 N-terminal domain (residues 9–393 of the polyprotein, with mutations Q132L and L270F) was cloned and expressed in
<italic>Escherichia coli</italic>
and the protein was subjected to crystallization trials. The crystals diffracted to 2.5 Å resolution and belonged to space group
<italic>P</italic>
6
<sub>2</sub>
or
<italic>P</italic>
6
<sub>4</sub>
, with unit-cell parameters
<italic>a</italic>
=
<italic>b</italic>
= 114.2,
<italic>c</italic>
= 61.0 Å, α = β = 90, γ = 120°. Each asymmetric unit contained one molecule.</p>
</abstract>
<kwd-group>
<kwd>coronaviruses</kwd>
<kwd>avian infectious bronchitis virus</kwd>
<kwd>nonstructural proteins</kwd>
<kwd>nsp2</kwd>
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<name sortKey="Yang, Anqi" sort="Yang, Anqi" uniqKey="Yang A" first="Anqi" last="Yang">Anqi Yang</name>
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<name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name>
<name sortKey="Wei, Lei" sort="Wei, Lei" uniqKey="Wei L" first="Lei" last="Wei">Lei Wei</name>
<name sortKey="Xu, Yuanyuan" sort="Xu, Yuanyuan" uniqKey="Xu Y" first="Yuanyuan" last="Xu">Yuanyuan Xu</name>
<name sortKey="Zhao, Weiran" sort="Zhao, Weiran" uniqKey="Zhao W" first="Weiran" last="Zhao">Weiran Zhao</name>
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