Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection
Identifieur interne : 000142 ( Ncbi/Merge ); précédent : 000141; suivant : 000143Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection
Auteurs : Sandra Thibivilliers [États-Unis] ; Trupti Joshi [États-Unis] ; Kimberly B. Campbell [États-Unis] ; Brian Scheffler [États-Unis] ; Dong Xu [États-Unis] ; Bret Cooper [États-Unis] ; Henry T. Nguyen [États-Unis] ; Gary Stacey [États-Unis]Source :
- BMC Plant Biology [ 1471-2229 ] ; 2009.
Abstract
A subtractive bean cDNA library composed of 10,581 unisequences was constructed and enriched in sequences regulated by either bean rust race 41, a virulent strain, or race 49, an avirulent strain on cultivar Early Gallatin carrying the resistance gene
The enrichment of the public database with over 6,000 bean ESTs significantly adds to the genomic resources available for this important crop plant. The analysis of these genes in response to bean rust infection provides a foundation for further studies of the mechanism of fungal disease resistance. The expression pattern of 90 bean genes upon rust infection shares several features with other legumes infected by biotrophic fungi. This finding suggests that the
Url:
DOI: 10.1186/1471-2229-9-46
PubMed: 19397807
PubMed Central: 2684537
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PMC:2684537Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Generation of <italic>Phaseolus vulgaris </italic>
ESTs and investigation of their regulation upon <italic>Uromyces appendiculatus </italic>
infection</title>
<author><name sortKey="Thibivilliers, Sandra" sort="Thibivilliers, Sandra" uniqKey="Thibivilliers S" first="Sandra" last="Thibivilliers">Sandra Thibivilliers</name>
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<author><name sortKey="Nguyen, Henry T" sort="Nguyen, Henry T" uniqKey="Nguyen H" first="Henry T" last="Nguyen">Henry T. Nguyen</name>
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<author><name sortKey="Stacey, Gary" sort="Stacey, Gary" uniqKey="Stacey G" first="Gary" last="Stacey">Gary Stacey</name>
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<series><title level="j">BMC Plant Biology</title>
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<imprint><date when="2009">2009</date>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p><italic>Phaseolus vulgaris </italic>
(common bean) is the second most important legume crop in the world after soybean. Consequently, yield losses due to fungal infection, like <italic>Uromyces appendiculatus </italic>
(bean rust), have strong consequences. Several resistant genes were identified that confer resistance to bean rust infection. However, the downstream genes and mechanisms involved in bean resistance to infection are poorly characterized.</p>
</sec>
<sec><title>Results</title>
<p>A subtractive bean cDNA library composed of 10,581 unisequences was constructed and enriched in sequences regulated by either bean rust race 41, a virulent strain, or race 49, an avirulent strain on cultivar Early Gallatin carrying the resistance gene <italic>Ur-4</italic>
. The construction of this library allowed the identification of 6,202 new bean ESTs, significantly adding to the available sequences for this plant. Regulation of selected bean genes in response to bean rust infection was confirmed by qRT-PCR. Plant gene expression was similar for both race 41 and 49 during the first 48 hours of the infection process but varied significantly at the later time points (72–96 hours after inoculation) mainly due to the presence of the <italic>Avr4 </italic>
gene in the race 49 leading to a hypersensitive response in the bean plants. A biphasic pattern of gene expression was observed for several genes regulated in response to fungal infection.</p>
</sec>
<sec><title>Conclusion</title>
<p>The enrichment of the public database with over 6,000 bean ESTs significantly adds to the genomic resources available for this important crop plant. The analysis of these genes in response to bean rust infection provides a foundation for further studies of the mechanism of fungal disease resistance. The expression pattern of 90 bean genes upon rust infection shares several features with other legumes infected by biotrophic fungi. This finding suggests that the <italic>P. vulgaris</italic>
-<italic>U. appendiculatus </italic>
pathosystem could serve as a model to explore legume-rust interaction.</p>
</sec>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Plant Biol</journal-id>
<journal-title>BMC Plant Biology</journal-title>
<issn pub-type="epub">1471-2229</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">19397807</article-id>
<article-id pub-id-type="pmc">2684537</article-id>
<article-id pub-id-type="publisher-id">1471-2229-9-46</article-id>
<article-id pub-id-type="doi">10.1186/1471-2229-9-46</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Generation of <italic>Phaseolus vulgaris </italic>
ESTs and investigation of their regulation upon <italic>Uromyces appendiculatus </italic>
infection</article-title>
</title-group>
<contrib-group><contrib id="A1" contrib-type="author"><name><surname>Thibivilliers</surname>
<given-names>Sandra</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>st5y7@mizzou.edu</email>
</contrib>
<contrib id="A2" contrib-type="author"><name><surname>Joshi</surname>
<given-names>Trupti</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>joshitr@missouri.edu</email>
</contrib>
<contrib id="A3" contrib-type="author"><name><surname>Campbell</surname>
<given-names>Kimberly B</given-names>
</name>
<xref ref-type="aff" rid="I3">3</xref>
<email>campbelk@ba.ars.usda.go</email>
</contrib>
<contrib id="A4" contrib-type="author"><name><surname>Scheffler</surname>
<given-names>Brian</given-names>
</name>
<xref ref-type="aff" rid="I4">4</xref>
<email>bscheffler@msa-stoneville.ars.usda.gov</email>
</contrib>
<contrib id="A5" contrib-type="author"><name><surname>Xu</surname>
<given-names>Dong</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>xudong@missouri.edu</email>
</contrib>
<contrib id="A6" contrib-type="author"><name><surname>Cooper</surname>
<given-names>Bret</given-names>
</name>
<xref ref-type="aff" rid="I3">3</xref>
<email>cooperb@ba.ars.usda.gov</email>
</contrib>
<contrib id="A7" contrib-type="author"><name><surname>Nguyen</surname>
<given-names>Henry T</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>nguyenhenry@missouri.edu</email>
</contrib>
<contrib id="A8" corresp="yes" contrib-type="author"><name><surname>Stacey</surname>
<given-names>Gary</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>staceyg@missouri.edu</email>
</contrib>
</contrib-group>
<aff id="I1"><label>1</label>
National Center for Soybean Biotechnology, Center for Sustainable Energy, Divisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, MO, 65211, USA</aff>
<aff id="I2"><label>2</label>
Computer Science Department and Christopher S Bond Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA</aff>
<aff id="I3"><label>3</label>
Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, 20705, USA</aff>
<aff id="I4"><label>4</label>
MSA Genomics Laboratory, USDA-ARS, Stoneville, MS, 38776, USA</aff>
<pub-date pub-type="collection"><year>2009</year>
</pub-date>
<pub-date pub-type="epub"><day>27</day>
<month>4</month>
<year>2009</year>
</pub-date>
<volume>9</volume>
<fpage>46</fpage>
<lpage>46</lpage>
<ext-link ext-link-type="uri" xlink:href="http://www.biomedcentral.com/1471-2229/9/46"></ext-link>
<history><date date-type="received"><day>17</day>
<month>11</month>
<year>2008</year>
</date>
<date date-type="accepted"><day>27</day>
<month>4</month>
<year>2009</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2009 Thibivilliers et al; licensee BioMed Central Ltd.</copyright-statement>
<copyright-year>2009</copyright-year>
<copyright-holder>Thibivilliers et al; licensee BioMed Central Ltd.</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0"></ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
<pmc-comment>
Thibivilliers
Sandra
st5y7@mizzou.edu
Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection
2009 BMC Plant Biology 9(1): 46-. (2009) 1471-2229(2009)9:1<46> urn:ISSN:1471-2229 </pmc-comment>
</license>
</permissions>
<abstract><sec><title>Background</title>
<p><italic>Phaseolus vulgaris </italic>
(common bean) is the second most important legume crop in the world after soybean. Consequently, yield losses due to fungal infection, like <italic>Uromyces appendiculatus </italic>
(bean rust), have strong consequences. Several resistant genes were identified that confer resistance to bean rust infection. However, the downstream genes and mechanisms involved in bean resistance to infection are poorly characterized.</p>
</sec>
<sec><title>Results</title>
<p>A subtractive bean cDNA library composed of 10,581 unisequences was constructed and enriched in sequences regulated by either bean rust race 41, a virulent strain, or race 49, an avirulent strain on cultivar Early Gallatin carrying the resistance gene <italic>Ur-4</italic>
. The construction of this library allowed the identification of 6,202 new bean ESTs, significantly adding to the available sequences for this plant. Regulation of selected bean genes in response to bean rust infection was confirmed by qRT-PCR. Plant gene expression was similar for both race 41 and 49 during the first 48 hours of the infection process but varied significantly at the later time points (72–96 hours after inoculation) mainly due to the presence of the <italic>Avr4 </italic>
gene in the race 49 leading to a hypersensitive response in the bean plants. A biphasic pattern of gene expression was observed for several genes regulated in response to fungal infection.</p>
</sec>
<sec><title>Conclusion</title>
<p>The enrichment of the public database with over 6,000 bean ESTs significantly adds to the genomic resources available for this important crop plant. The analysis of these genes in response to bean rust infection provides a foundation for further studies of the mechanism of fungal disease resistance. The expression pattern of 90 bean genes upon rust infection shares several features with other legumes infected by biotrophic fungi. This finding suggests that the <italic>P. vulgaris</italic>
-<italic>U. appendiculatus </italic>
pathosystem could serve as a model to explore legume-rust interaction.</p>
</sec>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Thibivilliers, Sandra" sort="Thibivilliers, Sandra" uniqKey="Thibivilliers S" first="Sandra" last="Thibivilliers">Sandra Thibivilliers</name>
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<name sortKey="Campbell, Kimberly B" sort="Campbell, Kimberly B" uniqKey="Campbell K" first="Kimberly B" last="Campbell">Kimberly B. Campbell</name>
<name sortKey="Cooper, Bret" sort="Cooper, Bret" uniqKey="Cooper B" first="Bret" last="Cooper">Bret Cooper</name>
<name sortKey="Joshi, Trupti" sort="Joshi, Trupti" uniqKey="Joshi T" first="Trupti" last="Joshi">Trupti Joshi</name>
<name sortKey="Nguyen, Henry T" sort="Nguyen, Henry T" uniqKey="Nguyen H" first="Henry T" last="Nguyen">Henry T. Nguyen</name>
<name sortKey="Scheffler, Brian" sort="Scheffler, Brian" uniqKey="Scheffler B" first="Brian" last="Scheffler">Brian Scheffler</name>
<name sortKey="Stacey, Gary" sort="Stacey, Gary" uniqKey="Stacey G" first="Gary" last="Stacey">Gary Stacey</name>
<name sortKey="Xu, Dong" sort="Xu, Dong" uniqKey="Xu D" first="Dong" last="Xu">Dong Xu</name>
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