Comparison of electron microscopy, enzyme-linked immunosorbent assay, solid-phase radioimmunoassay, and indirect immunofluorescence for detection of human rotavirus antigen in faeces.
Identifieur interne : 001774 ( Main/Merge ); précédent : 001773; suivant : 001775Comparison of electron microscopy, enzyme-linked immunosorbent assay, solid-phase radioimmunoassay, and indirect immunofluorescence for detection of human rotavirus antigen in faeces.
Auteurs : C J Birch ; N I Lehmann ; A J Hawker ; J A Marshall ; I D GustSource :
- Journal of Clinical Pathology [ 0021-9746 ] ; 1979-07.
English descriptors
- Teeft :
- Birch, Bovine albumin, Bryden, Carbonate buffer, Centrifugation, Clinical microbiology, Clinical pathology, Coating antibody, Electron microscope, Electron microscopy, Elisa, Elisa system, Elisa test, Equal volume, Faecal, Faecal extracts, Fairfield hospital, Fetal calf serum, Fluorescent virus precipitin test, Fluorescing cells, Gastroenteritis, Human agent, Human rotavirus, Hyperimmune rabbit serum, Iiffor detection, Immunofluorescence, Immunosorbent assay, Indirect immunofluorescence, Large numbers, Microtiter radioimmunoassay, Neutralisation test, Normal saline, Other agents, Radioimmunoassay, Room temperature, Rotavirus, Rotavirus antigen, Rotavirus infections, Rotavirus particles, Rotavirus serum, Routine diagnosis, Routine examination, Serum wells, Several weeks, Sodium azide, Viral antigen, Virology department, Virus particles, Yolken.
Abstract
Four techniques were compared for their practicability, speed, and sensitivity for the detection of human rotavirus. Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) were found to be the most sensitive means of identifying rotavirus, and, once processed, up to 40 specimens could be examined daily. Electron microscopy, although less sensitive than these techniques, had the advantage of being able to detect other viral agents present in faecal extracts. Indirect immunofluorescence failed to detect rotavirus as often as the other three methods. In laboratories where routine examination of faecal specimens from patients with gastroenteritis is required, ELISA and RIA are useful alternatives to electron microscopy.
Url:
DOI: 10.1136/jcp.32.7.700
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Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) were found to be the most sensitive means of identifying rotavirus, and, once processed, up to 40 specimens could be examined daily. Electron microscopy, although less sensitive than these techniques, had the advantage of being able to detect other viral agents present in faecal extracts. Indirect immunofluorescence failed to detect rotavirus as often as the other three methods. In laboratories where routine examination of faecal specimens from patients with gastroenteritis is required, ELISA and RIA are useful alternatives to electron microscopy.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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