Computational analysis and determination of a highly conserved surface exposed segment in H5N1 avian flu and H1N1 swine flu neuraminidase
Identifieur interne : 000D28 ( Main/Merge ); précédent : 000D27; suivant : 000D29Computational analysis and determination of a highly conserved surface exposed segment in H5N1 avian flu and H1N1 swine flu neuraminidase
Auteurs : Ambarnil Ghosh [Inde] ; Ashesh Nandy [Inde] ; Papiya Nandy [Inde]Source :
- BMC Structural Biology [ 1472-6807 ] ; 2010.
Abstract
Catalytic activity of influenza neuraminidase (NA) facilitates elution of progeny virions from infected cells and prevents their self-aggregation mediated by the catalytic site located in the body region. Research on the active site of the molecule has led to development of effective inhibitors like oseltamivir, zanamivir etc, but the high rate of mutation and interspecies reassortment in viral sequences and the recent reports of oseltamivir resistant strains underlines the importance of determining additional target sites for developing future antiviral compounds. In a recent computational study of 173 H5N1 NA gene sequences we had identified a 50-base highly conserved region in 3'-terminal end of the NA gene.
We extend the graphical and numerical analyses to a larger number of H5N1 NA sequences (514) and H1N1 swine flu sequences (425) accessed from GenBank. We use a 2D graphical representation model for the gene sequences and a Graphical Sliding Window Method (GSWM) for protein sequences scanning the sequences as a block of 16 amino acids at a time. Using a protein sequence descriptor defined in our model, the protein sliding scan method allowed us to compare the different strains for block level variability, which showed significant statistical correlation to average solvent accessibility of the residue blocks; single amino acid position variability results in no correlation, indicating the impact of stretch variability in chemical environment. Close to the C-terminal end the GSWM showed less descriptor-variability with increased average solvent accessibility (ASA) that is also supported by conserved predicted secondary structure of 3' terminal RNA and visual evidence from 3D crystallographic structure.
The identified terminal segment, strongly conserved in both RNA and protein sequences, is especially significant as it is surface exposed and structural chemistry reveals the probable role of this stretch in tetrameric stabilization. It could also participate in other biological processes associated with conserved surface residues. A RNA double hairpin secondary structure found in this segment in a majority of the H5N1 strains also supports this observation. In this paper we propose this conserved region as a probable site for designing inhibitors for broad-spectrum pandemic control of flu viruses with similar NA structure.
Url:
DOI: 10.1186/1472-6807-10-6
PubMed: 20170556
PubMed Central: 2836360
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Catalytic activity of influenza neuraminidase (NA) facilitates elution of progeny virions from infected cells and prevents their self-aggregation mediated by the catalytic site located in the body region. Research on the active site of the molecule has led to development of effective inhibitors like oseltamivir, zanamivir etc, but the high rate of mutation and interspecies reassortment in viral sequences and the recent reports of oseltamivir resistant strains underlines the importance of determining additional target sites for developing future antiviral compounds. In a recent computational study of 173 H5N1 NA gene sequences we had identified a 50-base highly conserved region in 3'-terminal end of the NA gene.</p>
</sec>
<sec><title>Results</title>
<p>We extend the graphical and numerical analyses to a larger number of H5N1 NA sequences (514) and H1N1 swine flu sequences (425) accessed from GenBank. We use a 2D graphical representation model for the gene sequences and a Graphical Sliding Window Method (GSWM) for protein sequences scanning the sequences as a block of 16 amino acids at a time. Using a protein sequence descriptor defined in our model, the protein sliding scan method allowed us to compare the different strains for block level variability, which showed significant statistical correlation to average solvent accessibility of the residue blocks; single amino acid position variability results in no correlation, indicating the impact of stretch variability in chemical environment. Close to the C-terminal end the GSWM showed less descriptor-variability with increased average solvent accessibility (ASA) that is also supported by conserved predicted secondary structure of 3' terminal RNA and visual evidence from 3D crystallographic structure.</p>
</sec>
<sec><title>Conclusion</title>
<p>The identified terminal segment, strongly conserved in both RNA and protein sequences, is especially significant as it is surface exposed and structural chemistry reveals the probable role of this stretch in tetrameric stabilization. It could also participate in other biological processes associated with conserved surface residues. A RNA double hairpin secondary structure found in this segment in a majority of the H5N1 strains also supports this observation. In this paper we propose this conserved region as a probable site for designing inhibitors for broad-spectrum pandemic control of flu viruses with similar NA structure.</p>
</sec>
</div>
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<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Tumpey, Tm" uniqKey="Tumpey T">TM Tumpey</name>
</author>
<author><name sortKey="Basler, Cf" uniqKey="Basler C">CF Basler</name>
</author>
<author><name sortKey="Aguilar, Pv" uniqKey="Aguilar P">PV Aguilar</name>
</author>
<author><name sortKey="Zeng, H" uniqKey="Zeng H">H Zeng</name>
</author>
<author><name sortKey="Solorzano, A" uniqKey="Solorzano A">A Solorzano</name>
</author>
<author><name sortKey="Swayne, De" uniqKey="Swayne D">DE Swayne</name>
</author>
<author><name sortKey="Cox, Nj" uniqKey="Cox N">NJ Cox</name>
</author>
<author><name sortKey="Katz, Jm" uniqKey="Katz J">JM Katz</name>
</author>
<author><name sortKey="Taubenberger, Jk" uniqKey="Taubenberger J">JK Taubenberger</name>
</author>
<author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name>
</author>
<author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A Garcia-Sastre</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Haolum, J" uniqKey="Haolum J">J Haolum</name>
</author>
<author><name sortKey="Choung, Uk" uniqKey="Choung U">UK Choung</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lal, Sk" uniqKey="Lal S">SK Lal</name>
</author>
<author><name sortKey="Chow, Vtk" uniqKey="Chow V">VTK Chow</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="He, Cq" uniqKey="He C">CQ He</name>
</author>
<author><name sortKey="Xie, Zx" uniqKey="Xie Z">ZX Xie</name>
</author>
<author><name sortKey="Han, Gz" uniqKey="Han G">GZ Han</name>
</author>
<author><name sortKey="Dong, Jb" uniqKey="Dong J">JB Dong</name>
</author>
<author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name>
</author>
<author><name sortKey="Liu, Jb" uniqKey="Liu J">JB Liu</name>
</author>
<author><name sortKey="Ma, Ly" uniqKey="Ma L">LY Ma</name>
</author>
<author><name sortKey="Tang, Xf" uniqKey="Tang X">XF Tang</name>
</author>
<author><name sortKey="Liu, Xp" uniqKey="Liu X">XP Liu</name>
</author>
<author><name sortKey="Pang, Ys" uniqKey="Pang Y">YS Pang</name>
</author>
<author><name sortKey="Li, Gr" uniqKey="Li G">GR Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pasick, J" uniqKey="Pasick J">J Pasick</name>
</author>
<author><name sortKey="Handel, K" uniqKey="Handel K">K Handel</name>
</author>
<author><name sortKey="Robinson, J" uniqKey="Robinson J">J Robinson</name>
</author>
<author><name sortKey="Copps, J" uniqKey="Copps J">J Copps</name>
</author>
<author><name sortKey="Ridd, D" uniqKey="Ridd D">D Ridd</name>
</author>
<author><name sortKey="Hills, K" uniqKey="Hills K">K Hills</name>
</author>
<author><name sortKey="Kehler, H" uniqKey="Kehler H">H Kehler</name>
</author>
<author><name sortKey="Cottam Birt, C" uniqKey="Cottam Birt C">C Cottam-Birt</name>
</author>
<author><name sortKey="Neufeld, J" uniqKey="Neufeld J">J Neufeld</name>
</author>
<author><name sortKey="Berhane, Y" uniqKey="Berhane Y">Y Berhane</name>
</author>
<author><name sortKey="Czub, S" uniqKey="Czub S">S Czub</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Suarez, Dl" uniqKey="Suarez D">DL Suarez</name>
</author>
<author><name sortKey="Senne, Da" uniqKey="Senne D">DA Senne</name>
</author>
<author><name sortKey="Banks, J" uniqKey="Banks J">J Banks</name>
</author>
<author><name sortKey="Brown, Ih" uniqKey="Brown I">IH Brown</name>
</author>
<author><name sortKey="Essen, Sc" uniqKey="Essen S">SC Essen</name>
</author>
<author><name sortKey="Lee, Cw" uniqKey="Lee C">CW Lee</name>
</author>
<author><name sortKey="Manvell, Rj" uniqKey="Manvell R">RJ Manvell</name>
</author>
<author><name sortKey="Mathieu Benson, C" uniqKey="Mathieu Benson C">C Mathieu-Benson</name>
</author>
<author><name sortKey="Moreno, V" uniqKey="Moreno V">V Moreno</name>
</author>
<author><name sortKey="Pedersen, Jc" uniqKey="Pedersen J">JC Pedersen</name>
</author>
<author><name sortKey="Panigrahy, P" uniqKey="Panigrahy P">P Panigrahy</name>
</author>
<author><name sortKey="Rojas, H" uniqKey="Rojas H">H Rojas</name>
</author>
<author><name sortKey="Spackman, E" uniqKey="Spackman E">E Spackman</name>
</author>
<author><name sortKey="Alexander, Dj" uniqKey="Alexander D">DJ Alexander</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gibbs, Mj" uniqKey="Gibbs M">MJ Gibbs</name>
</author>
<author><name sortKey="Armstrong, Js" uniqKey="Armstrong J">JS Armstrong</name>
</author>
<author><name sortKey="Gibbs, Aj" uniqKey="Gibbs A">AJ Gibbs</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bergmann, M" uniqKey="Bergmann M">M Bergmann</name>
</author>
<author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A Garcia-Sastre</name>
</author>
<author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Niman, H" uniqKey="Niman H">H Niman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ghosh, A" uniqKey="Ghosh A">A Ghosh</name>
</author>
<author><name sortKey="Nandy, A" uniqKey="Nandy A">A Nandy</name>
</author>
<author><name sortKey="Nandy, P" uniqKey="Nandy P">P Nandy</name>
</author>
<author><name sortKey="Gute, Bd" uniqKey="Gute B">BD Gute</name>
</author>
<author><name sortKey="Basak, Sc" uniqKey="Basak S">SC Basak</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Jefferson, T" uniqKey="Jefferson T">T Jefferson</name>
</author>
<author><name sortKey="Demicheli, V" uniqKey="Demicheli V">V Demicheli</name>
</author>
<author><name sortKey="Di Pietrantonj, C" uniqKey="Di Pietrantonj C">C Di Pietrantonj</name>
</author>
<author><name sortKey="Rivetti, D" uniqKey="Rivetti D">D Rivetti</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moscona, A" uniqKey="Moscona A">A Moscona</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yen, Hl" uniqKey="Yen H">HL Yen</name>
</author>
<author><name sortKey="Ilyushina, Na" uniqKey="Ilyushina N">NA Ilyushina</name>
</author>
<author><name sortKey="Salomon, R" uniqKey="Salomon R">R Salomon</name>
</author>
<author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E Hoffmann</name>
</author>
<author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name>
</author>
<author><name sortKey="Govorkova, Ea" uniqKey="Govorkova E">EA Govorkova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kiso, M" uniqKey="Kiso M">M Kiso</name>
</author>
<author><name sortKey="Mitamura, K" uniqKey="Mitamura K">K Mitamura</name>
</author>
<author><name sortKey="Sakai Tagawa, Y" uniqKey="Sakai Tagawa Y">Y Sakai-Tagawa</name>
</author>
<author><name sortKey="Shiraishi, K" uniqKey="Shiraishi K">K Shiraishi</name>
</author>
<author><name sortKey="Kawakami, C" uniqKey="Kawakami C">C Kawakami</name>
</author>
<author><name sortKey="Kimura, K" uniqKey="Kimura K">K Kimura</name>
</author>
<author><name sortKey="Hayden, Fg" uniqKey="Hayden F">FG Hayden</name>
</author>
<author><name sortKey="Sugaya, N" uniqKey="Sugaya N">N Sugaya</name>
</author>
<author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reece, Pa" uniqKey="Reece P">PA Reece</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gubareva, Lv" uniqKey="Gubareva L">LV Gubareva</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hurt, Ac" uniqKey="Hurt A">AC Hurt</name>
</author>
<author><name sortKey="Holien, Jk" uniqKey="Holien J">JK Holien</name>
</author>
<author><name sortKey="Parker, M" uniqKey="Parker M">M Parker</name>
</author>
<author><name sortKey="Kelso, A" uniqKey="Kelso A">A Kelso</name>
</author>
<author><name sortKey="Barr, Ig" uniqKey="Barr I">IG Barr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nandy, A" uniqKey="Nandy A">A Nandy</name>
</author>
<author><name sortKey="Basak, Sc" uniqKey="Basak S">SC Basak</name>
</author>
<author><name sortKey="Gute, Bd" uniqKey="Gute B">BD Gute</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hamori, E" uniqKey="Hamori E">E Hamori</name>
</author>
<author><name sortKey="Ruskin, J" uniqKey="Ruskin J">J Ruskin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gates, Ma" uniqKey="Gates M">MA Gates</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nandy, A" uniqKey="Nandy A">A Nandy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Leong, Pm" uniqKey="Leong P">PM Leong</name>
</author>
<author><name sortKey="Morgenthaler, S" uniqKey="Morgenthaler S">S Morgenthaler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nandy, A" uniqKey="Nandy A">A Nandy</name>
</author>
<author><name sortKey="Harle, M" uniqKey="Harle M">M Harle</name>
</author>
<author><name sortKey="Basak, Sc" uniqKey="Basak S">SC Basak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liao, B" uniqKey="Liao B">B Liao</name>
</author>
<author><name sortKey="Xiang, X" uniqKey="Xiang X">X Xiang</name>
</author>
<author><name sortKey="Zhu, W" uniqKey="Zhu W">W Zhu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liao, B" uniqKey="Liao B">B Liao</name>
</author>
<author><name sortKey="Tan, M" uniqKey="Tan M">M Tan</name>
</author>
<author><name sortKey="Ding, K" uniqKey="Ding K">K Ding</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Larionov, S" uniqKey="Larionov S">S Larionov</name>
</author>
<author><name sortKey="Loskutov, A" uniqKey="Loskutov A">A Loskutov</name>
</author>
<author><name sortKey="Ryadchenko, E" uniqKey="Ryadchenko E">E Ryadchenko</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Randic, M" uniqKey="Randic M">M Randic'</name>
</author>
<author><name sortKey="Zupan, J" uniqKey="Zupan J">J Zupan</name>
</author>
<author><name sortKey="Balaban, At" uniqKey="Balaban A">AT Balaban</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Randi, M" uniqKey="Randi M">M Randić</name>
</author>
<author><name sortKey="Novi, M" uniqKey="Novi M">M Novič</name>
</author>
<author><name sortKey="Vra Ko, M" uniqKey="Vra Ko M">M Vračko</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, Dc" uniqKey="Li D">DC Li</name>
</author>
<author><name sortKey="Yua, X" uniqKey="Yua X">X Yua</name>
</author>
<author><name sortKey="Yanga, L" uniqKey="Yanga L">L Yanga</name>
</author>
<author><name sortKey="Zhengb, X" uniqKey="Zhengb X">X Zhengb</name>
</author>
<author><name sortKey="Wanga, Z" uniqKey="Wanga Z">Z Wanga</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bai, F" uniqKey="Bai F">F Bai</name>
</author>
<author><name sortKey="Zhu, W" uniqKey="Zhu W">W Zhu</name>
</author>
<author><name sortKey="Wang, T" uniqKey="Wang T">T Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Estrada, E" uniqKey="Estrada E">E Estrada</name>
</author>
<author><name sortKey="Uriarte, E" uniqKey="Uriarte E">E Uriarte</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez Diaz, H" uniqKey="Gonzalez Diaz H">H González-Díaz</name>
</author>
<author><name sortKey="Vilar, S" uniqKey="Vilar S">S Vilar</name>
</author>
<author><name sortKey="Santana, L" uniqKey="Santana L">L Santana</name>
</author>
<author><name sortKey="Uriarte, E" uniqKey="Uriarte E">E Uriarte</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Aguero Chapin, G" uniqKey="Aguero Chapin G">G Aguero-Chapin</name>
</author>
<author><name sortKey="Varona Santos, J" uniqKey="Varona Santos J">J Varona-Santos</name>
</author>
<author><name sortKey="De La Riva, Ga" uniqKey="De La Riva G">GA de la Riva</name>
</author>
<author><name sortKey="Antunes, A" uniqKey="Antunes A">A Antunes</name>
</author>
<author><name sortKey="Gonzalez Villa, T" uniqKey="Gonzalez Villa T">T Gonzalez-Villa</name>
</author>
<author><name sortKey="Uriarte, E" uniqKey="Uriarte E">E Uriarte</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez Diaz, H" uniqKey="Gonzalez Diaz H">H Gonzalez-Diaz</name>
</author>
<author><name sortKey="Prado Prado, F" uniqKey="Prado Prado F">F Prado-Prado</name>
</author>
<author><name sortKey="Ubeira, Fm" uniqKey="Ubeira F">FM Ubeira</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cruz Monteagudo, M" uniqKey="Cruz Monteagudo M">M Cruz-Monteagudo</name>
</author>
<author><name sortKey="Gonzalez Diaz, H" uniqKey="Gonzalez Diaz H">H González-Díaz</name>
</author>
<author><name sortKey="Borges, F" uniqKey="Borges F">F Borges</name>
</author>
<author><name sortKey="Dominguez, Er" uniqKey="Dominguez E">ER Dominguez</name>
</author>
<author><name sortKey="Cordeiro, Mn" uniqKey="Cordeiro M">MN Cordeiro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez Diaz, H" uniqKey="Gonzalez Diaz H">H González-Díaz</name>
</author>
<author><name sortKey="Gonzalez Diaz, Y" uniqKey="Gonzalez Diaz Y">Y González-Díaz</name>
</author>
<author><name sortKey="Santana, L" uniqKey="Santana L">L Santana</name>
</author>
<author><name sortKey="Ubeira, Fm" uniqKey="Ubeira F">FM Ubeira</name>
</author>
<author><name sortKey="Uriarte, E" uniqKey="Uriarte E">E Uriarte</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez Diaz, H" uniqKey="Gonzalez Diaz H">H González-Díaz</name>
</author>
<author><name sortKey="Perez Montoto, Lg" uniqKey="Perez Montoto L">LG Pérez-Montoto</name>
</author>
<author><name sortKey="Duardo Sanchez, A" uniqKey="Duardo Sanchez A">A Duardo-Sanchez</name>
</author>
<author><name sortKey="Paniagua, E" uniqKey="Paniagua E">E Paniagua</name>
</author>
<author><name sortKey="Vazquez Prieto, S" uniqKey="Vazquez Prieto S">S Vázquez-Prieto</name>
</author>
<author><name sortKey="Vilas, R" uniqKey="Vilas R">R Vilas</name>
</author>
<author><name sortKey="Dea Ayuela, Ma" uniqKey="Dea Ayuela M">MA Dea-Ayuela</name>
</author>
<author><name sortKey="Bolas Fernandez, F" uniqKey="Bolas Fernandez F">F Bolas-Fernández</name>
</author>
<author><name sortKey="Munteanu, Cr" uniqKey="Munteanu C">CR Munteanu</name>
</author>
<author><name sortKey="Dorado, J" uniqKey="Dorado J">J Dorado</name>
</author>
<author><name sortKey="Costas, J" uniqKey="Costas J">J Costas</name>
</author>
<author><name sortKey="Ubeira, Fm" uniqKey="Ubeira F">FM Ubeira</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nandy, A" uniqKey="Nandy A">A Nandy</name>
</author>
<author><name sortKey="Ghosh, A" uniqKey="Ghosh A">A Ghosh</name>
</author>
<author><name sortKey="Nandy, P" uniqKey="Nandy P">P Nandy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Raychaudhury, C" uniqKey="Raychaudhury C">C Raychaudhury</name>
</author>
<author><name sortKey="Nandy, A" uniqKey="Nandy A">A Nandy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="M Randi, M" uniqKey="M Randi M">M M Randić</name>
</author>
<author><name sortKey="Vra Ko, M" uniqKey="Vra Ko M">M Vračko</name>
</author>
<author><name sortKey="Nandy, A" uniqKey="Nandy A">A Nandy</name>
</author>
<author><name sortKey="Basak, Sc" uniqKey="Basak S">SC Basak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nufer, O" uniqKey="Nufer O">O Nufer</name>
</author>
<author><name sortKey="Guldbrandsen, S" uniqKey="Guldbrandsen S">S Guldbrandsen</name>
</author>
<author><name sortKey="Degen, M" uniqKey="Degen M">M Degen</name>
</author>
<author><name sortKey="Kappeler, F" uniqKey="Kappeler F">F Kappeler</name>
</author>
<author><name sortKey="Paccaud, J P" uniqKey="Paccaud J">J-P Paccaud</name>
</author>
<author><name sortKey="Tani, K" uniqKey="Tani K">K Tani</name>
</author>
<author><name sortKey="Hauri, H P" uniqKey="Hauri H">H-P Hauri</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Seale, Jw" uniqKey="Seale J">JW Seale</name>
</author>
<author><name sortKey="Horowitz, Pm" uniqKey="Horowitz P">PM Horowitz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nagano, H" uniqKey="Nagano H">H Nagano</name>
</author>
<author><name sortKey="Okuno, T" uniqKey="Okuno T">T Okuno</name>
</author>
<author><name sortKey="Mise, K" uniqKey="Mise K">K Mise</name>
</author>
<author><name sortKey="Furusawa, I" uniqKey="Furusawa I">I Furusawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gao, L" uniqKey="Gao L">L Gao</name>
</author>
<author><name sortKey="Tripathy, A" uniqKey="Tripathy A">A Tripathy</name>
</author>
<author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name>
</author>
<author><name sortKey="Meissner, G" uniqKey="Meissner G">G Meissner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goto, H" uniqKey="Goto H">H Goto</name>
</author>
<author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, X" uniqKey="Li X">X Li</name>
</author>
<author><name sortKey="Fang, F" uniqKey="Fang F">F Fang</name>
</author>
<author><name sortKey="Song, Y" uniqKey="Song Y">Y Song</name>
</author>
<author><name sortKey="Yan, H" uniqKey="Yan H">H Yan</name>
</author>
<author><name sortKey="Chang, H" uniqKey="Chang H">H Chang</name>
</author>
<author><name sortKey="Sun, S" uniqKey="Sun S">S Sun</name>
</author>
<author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nandy, A" uniqKey="Nandy A">A Nandy</name>
</author>
<author><name sortKey="Basak, Sc" uniqKey="Basak S">SC Basak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nandy, A" uniqKey="Nandy A">A Nandy</name>
</author>
<author><name sortKey="Nandy, P" uniqKey="Nandy P">P Nandy</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Zuker, M" uniqKey="Zuker M">M Zuker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mathews, Dh" uniqKey="Mathews D">DH Mathews</name>
</author>
<author><name sortKey="Sabina, J" uniqKey="Sabina J">J Sabina</name>
</author>
<author><name sortKey="Zuker, M" uniqKey="Zuker M">M Zuker</name>
</author>
<author><name sortKey="Turner, Dh" uniqKey="Turner D">DH Turner</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Russell, Rj" uniqKey="Russell R">RJ Russell</name>
</author>
<author><name sortKey="Haire, Lf" uniqKey="Haire L">LF Haire</name>
</author>
<author><name sortKey="Stevens, Dj" uniqKey="Stevens D">DJ Stevens</name>
</author>
<author><name sortKey="Collins, Pj" uniqKey="Collins P">PJ Collins</name>
</author>
<author><name sortKey="Lin, Yp" uniqKey="Lin Y">YP Lin</name>
</author>
<author><name sortKey="Blackburn, Gm" uniqKey="Blackburn G">GM Blackburn</name>
</author>
<author><name sortKey="Hay, Aj" uniqKey="Hay A">AJ Hay</name>
</author>
<author><name sortKey="Gamblin, Sj" uniqKey="Gamblin S">SJ Gamblin</name>
</author>
<author><name sortKey="Skehel, Jj" uniqKey="Skehel J">JJ Skehel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Delano" uniqKey="Delano">DeLano</name>
</author>
<author><name sortKey="Warren, L" uniqKey="Warren L">L Warren</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Baudin, F" uniqKey="Baudin F">F Baudin</name>
</author>
<author><name sortKey="Bach, C" uniqKey="Bach C">C Bach</name>
</author>
<author><name sortKey="Cusack, S" uniqKey="Cusack S">S Cusack</name>
</author>
<author><name sortKey="Ruigrok, Rwh" uniqKey="Ruigrok R">RWH Ruigrok</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pritlove, Dc" uniqKey="Pritlove D">DC Pritlove</name>
</author>
<author><name sortKey="Fodor, E" uniqKey="Fodor E">E Fodor</name>
</author>
<author><name sortKey="Seongt, Bl" uniqKey="Seongt B">BL Seongt</name>
</author>
<author><name sortKey="Brownlee, Gg" uniqKey="Brownlee G">GG Brownlee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fodor, E" uniqKey="Fodor E">E Fodor</name>
</author>
<author><name sortKey="Pritlove, Dc" uniqKey="Pritlove D">DC Pritlove</name>
</author>
<author><name sortKey="Brownlee, Gg" uniqKey="Brownlee G">GG Brownlee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="West, Rb" uniqKey="West R">RB West</name>
</author>
<author><name sortKey="Lieber, Mr" uniqKey="Lieber M">MR Lieber</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Casey, Jl" uniqKey="Casey J">JL Casey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, S" uniqKey="Yang S">S Yang</name>
</author>
<author><name sortKey="Temin, Hm" uniqKey="Temin H">HM Temin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pal, A" uniqKey="Pal A">A Pal</name>
</author>
<author><name sortKey="Chakrabarti, P" uniqKey="Chakrabarti P">P Chakrabarti</name>
</author>
<author><name sortKey="Bahadur, R" uniqKey="Bahadur R">R Bahadur</name>
</author>
<author><name sortKey="Rodier, F" uniqKey="Rodier F">F Rodier</name>
</author>
<author><name sortKey="Janin, J" uniqKey="Janin J">J Janin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hardy, Ja" uniqKey="Hardy J">JA Hardy</name>
</author>
<author><name sortKey="Lam, J" uniqKey="Lam J">J Lam</name>
</author>
<author><name sortKey="Nguyen, Jt" uniqKey="Nguyen J">JT Nguyen</name>
</author>
<author><name sortKey="O Brien, T" uniqKey="O Brien T">T O'Brien</name>
</author>
<author><name sortKey="Wells, Ja" uniqKey="Wells J">JA Wells</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stewart, R" uniqKey="Stewart R">R Stewart</name>
</author>
<author><name sortKey="Zissimopoulos, S" uniqKey="Zissimopoulos S">S Zissimopoulos</name>
</author>
<author><name sortKey="Lai, Fa" uniqKey="Lai F">FA Lai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="May, Lt" uniqKey="May L">LT May</name>
</author>
<author><name sortKey="Leach, K" uniqKey="Leach K">K Leach</name>
</author>
<author><name sortKey="Sexton, Pm" uniqKey="Sexton P">PM Sexton</name>
</author>
<author><name sortKey="Christopoulos, A" uniqKey="Christopoulos A">A Christopoulos</name>
</author>
</analytic>
</biblStruct>
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