Biodegradable Nanoparticle-Entrapped Vaccine Induces Cross-Protective Immune Response against a Virulent Heterologous Respiratory Viral Infection in Pigs
Identifieur interne : 000B85 ( Main/Exploration ); précédent : 000B84; suivant : 000B86Biodegradable Nanoparticle-Entrapped Vaccine Induces Cross-Protective Immune Response against a Virulent Heterologous Respiratory Viral Infection in Pigs
Auteurs : Varun Dwivedi [États-Unis] ; Cordelia Manickam [États-Unis] ; Basavaraj Binjawadagi [États-Unis] ; Dechamma Joyappa [Inde] ; Gourapura J. Renukaradhya [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Abstract
Biodegradable nanoparticle-based vaccine development research is unexplored in large animals and humans. In this study, we illustrated the efficacy of nanoparticle-entrapped UV-killed virus vaccine against an economically important respiratory viral disease of pigs called porcine reproductive and respiratory syndrome virus (PRRSV). We entrapped PLGA [poly (lactide-co-glycolides)] nanoparticles with killed PRRSV antigens (Nano-KAg) and detected its phagocytosis by pig alveolar macrophages. Single doses of Nano-KAg vaccine administered intranasally to pigs upregulated innate and PRRSV specific adaptive responses. In a virulent heterologous PRRSV challenge study, Nano-KAg vaccine significantly reduced the lung pathology and viremia, and the viral load in the lungs. Immunologically, enhanced innate and adaptive immune cell population and associated cytokines with decreased secretion of immunosuppressive mediators were observed at both mucosal sites and blood. In summary, we demonstrated the benefits of intranasal delivery of nanoparticle-based viral vaccine in eliciting cross-protective immune response in pigs, a potential large animal model.
Url:
DOI: 10.1371/journal.pone.0051794
PubMed: 23240064
PubMed Central: 3519908
Affiliations:
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Immune Response against a Virulent Heterologous Respiratory Viral Infection in Pigs</title><author><name sortKey="Dwivedi, Varun" sort="Dwivedi, Varun" uniqKey="Dwivedi V" first="Varun" last="Dwivedi">Varun Dwivedi</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, United States, and Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, United States, and Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Manickam, Cordelia" sort="Manickam, Cordelia" uniqKey="Manickam C" first="Cordelia" last="Manickam">Cordelia Manickam</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, United States, and Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, United States, and Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Binjawadagi, Basavaraj" sort="Binjawadagi, Basavaraj" uniqKey="Binjawadagi B" first="Basavaraj" last="Binjawadagi">Basavaraj Binjawadagi</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, United States, and Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, United States, and Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Joyappa, Dechamma" sort="Joyappa, Dechamma" uniqKey="Joyappa D" first="Dechamma" last="Joyappa">Dechamma Joyappa</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Foot and Mouth Disease Laboratory, Indian Veterinary Research Institute, Hebbal, Bengaluru, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Foot and Mouth Disease Laboratory, Indian Veterinary Research Institute, Hebbal, Bengaluru</wicri:regionArea><wicri:noRegion>Bengaluru</wicri:noRegion></affiliation></author><author><name sortKey="Renukaradhya, Gourapura J" sort="Renukaradhya, Gourapura J" uniqKey="Renukaradhya G" first="Gourapura J." last="Renukaradhya">Gourapura J. Renukaradhya</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, United States, and Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, United States, and Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Biodegradable nanoparticle-based vaccine development research is unexplored in large animals and humans. In this study, we illustrated the efficacy of nanoparticle-entrapped UV-killed virus vaccine against an economically important respiratory viral disease of pigs called porcine reproductive and respiratory syndrome virus (PRRSV). We entrapped PLGA [poly (lactide-co-glycolides)] nanoparticles with killed PRRSV antigens (Nano-KAg) and detected its phagocytosis by pig alveolar macrophages. Single doses of Nano-KAg vaccine administered intranasally to pigs upregulated innate and PRRSV specific adaptive responses. In a virulent heterologous PRRSV challenge study, Nano-KAg vaccine significantly reduced the lung pathology and viremia, and the viral load in the lungs. Immunologically, enhanced innate and adaptive immune cell population and associated cytokines with decreased secretion of immunosuppressive mediators were observed at both mucosal sites and blood. In summary, we demonstrated the benefits of intranasal delivery of nanoparticle-based viral vaccine in eliciting cross-protective immune response in pigs, a potential large animal model.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Aguado, Mt" uniqKey="Aguado M">MT Aguado</name></author><author><name sortKey="Lambert, Ph" uniqKey="Lambert P">PH Lambert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Park, Tg" uniqKey="Park T">TG Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hein, Wr" uniqKey="Hein W">WR Hein</name></author><author><name sortKey="Griebel, Pj" uniqKey="Griebel P">PJ Griebel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rogers, Cs" uniqKey="Rogers C">CS Rogers</name></author><author><name sortKey="Abraham, Wm" uniqKey="Abraham W">WM Abraham</name></author><author><name sortKey="Brogden, Ka" 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M">MP Murtaugh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Welsh, Rm" uniqKey="Welsh R">RM Welsh</name></author><author><name sortKey="Lin, My" uniqKey="Lin M">MY Lin</name></author><author><name sortKey="Lohman, Bl" uniqKey="Lohman B">BL Lohman</name></author><author><name sortKey="Varga, Sm" uniqKey="Varga S">SM Varga</name></author><author><name sortKey="Zarozinski, Cc" uniqKey="Zarozinski C">CC Zarozinski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dwivedi, V" uniqKey="Dwivedi V">V Dwivedi</name></author><author><name sortKey="Manickam, C" uniqKey="Manickam C">C Manickam</name></author><author><name sortKey="Patterson, R" uniqKey="Patterson R">R Patterson</name></author><author><name sortKey="Dodson, K" uniqKey="Dodson K">K Dodson</name></author><author><name sortKey="Weeman, M" uniqKey="Weeman M">M Weeman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ogra, Pl" uniqKey="Ogra P">PL Ogra</name></author><author><name sortKey="Karzon, Dt" uniqKey="Karzon D">DT Karzon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liew, Fy" uniqKey="Liew F">FY Liew</name></author><author><name sortKey="Russell, Sm" uniqKey="Russell S">SM Russell</name></author><author><name sortKey="Appleyard, G" uniqKey="Appleyard G">G Appleyard</name></author><author><name sortKey="Brand, Cm" uniqKey="Brand C">CM Brand</name></author><author><name sortKey="Beale, J" uniqKey="Beale J">J Beale</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Inde</li><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><country name="États-Unis"><region name="Ohio"><name sortKey="Dwivedi, Varun" sort="Dwivedi, Varun" uniqKey="Dwivedi V" first="Varun" last="Dwivedi">Varun Dwivedi</name></region><name sortKey="Binjawadagi, Basavaraj" sort="Binjawadagi, Basavaraj" uniqKey="Binjawadagi B" first="Basavaraj" last="Binjawadagi">Basavaraj Binjawadagi</name><name sortKey="Manickam, Cordelia" sort="Manickam, Cordelia" uniqKey="Manickam C" first="Cordelia" last="Manickam">Cordelia Manickam</name><name sortKey="Renukaradhya, Gourapura J" sort="Renukaradhya, Gourapura J" uniqKey="Renukaradhya G" first="Gourapura J." last="Renukaradhya">Gourapura J. Renukaradhya</name></country><country name="Inde"><noRegion><name sortKey="Joyappa, Dechamma" sort="Joyappa, Dechamma" uniqKey="Joyappa D" first="Dechamma" last="Joyappa">Dechamma Joyappa</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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