Isolation of Infectious Bronchitis, Infectious Bursal Disease and Avian Adenoviruses from Chickens with Nephritis‐Nephrosis Syndrome
Identifieur interne : 001723 ( Main/Exploration ); précédent : 001722; suivant : 001724Isolation of Infectious Bronchitis, Infectious Bursal Disease and Avian Adenoviruses from Chickens with Nephritis‐Nephrosis Syndrome
Auteurs : Manik Chandra [Inde] ; Gurkirpal Singh [Inde] ; Balwant Singh [Inde]Source :
- Zentralblatt für Veterinärmedizin Reihe B [ 0931-2021 ] ; 1980-06.
English descriptors
- Teeft :
- Adenovirus, Agar, Avian, Avian adenovirus, Balanced salt solution, Balwant singh, Bronchite infectieuse, Bronchitis, Bronchitis virus, Bronquitis infecciosa, Bursitis infecciosa, Cell culture, Cent goat serum, Clinical symptoms, Connecticut strain, Culture tubes, First passage, Growth medium, Incubation period, Infectious bronchitis, Infectious bronchitis virus, Infectious bursa1 disease, Kidney suspension, Lesion, Maintenance medium, Microscopic lesions, Nephritis, Nephrosis, Neutralization, Neutralization indices, Neutralization test, Peripheral wells, Precipitation test, Precipitin line, Punjab, Room temperature, Singh, Sont rkvklks, Standard procedure, Subacute glomerulonephritis, Such cases, Syndrome, Test adenovlrus, Veterinary pathology, Viral, Viral agents, Viral isolation, Virus.
Abstract
Attempts at viral isolation were made in chicken kidney cell culture using kidney suspension as inoculum from 39 cases exhibiting typical gross and microscopic lesions of the nephritis‐nephrosis syndrome. Eleven viral isolates were recovered. Five isolates were identified as infectious bronchitis virus and one isolate as infectious bursal disease virus by neutralization test with anti‐IB serum and anti‐IBD serum, respectively. The remaining five isolates were identified as avian adenovirus in the agar gel precipitation test using anti‐CELO serum.
Isolierung vom Virus der Infektiösen Bronchitis und der Infektiösen Bursitis sowie aviärer Adenoviren von Küken mit Nephritis‐Nephrose‐Syndrom Zur Virusisolierung wurden Kükennieren‐Zellkulturen mit Zellsuspensionen aus Nieren von 39 Küken beimpft, die für das Nephritis‐Nephrose‐Syndrom typische makroskopische und mikroskopische Veränderungen zeigten. Von 11 gewonnenen Virusisolaten konnten 5 als Infektiöse Bronchitisund 1 Isolat als Infektiöse Bursitis‐Viren im Neutralisationstest, die übrigen 5 Isolate als aviäre Adenoviren in der Agargelpräzipitation mittels entsprechender Antiseren nachgewiesen werden.
Aislamiento de virus de la bronquitis infecciosa, bursitis infecciosa y adenovirus aviares a partir de pollitos con síndrome nefritis‐nefrosis Para poder aislar virus, se inyectaron cultivos celulares de riñones de pollitos con suspensiones celulares de riñones de 39 pollitos, los cuales presentaban las modificaciones macro y microscópicas típicas del síndrome nefritis‐nefrosis. Die 11 aislamientos de virus obtenidos, se pudieron identificar 5 como virus de la bronquitis infecciosa y 1 aislamiento como virus de la bursitis infecciosa en la prueba de neutralización, mientras que los 5 aislamientos restantes como adenovirus aviares en la precipitación en gelosa de agar mediante los antisueros correspondientes.
Url:
DOI: 10.1111/j.1439-0450.1980.tb01702.x
Affiliations:
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- to stream Istex, to step Corpus: 000402
- to stream Istex, to step Curation: 000382
- to stream Istex, to step Checkpoint: 000830
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- to stream Main, to step Curation: 001723
Le document en format XML
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<term>Cent goat serum</term>
<term>Clinical symptoms</term>
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<front><div type="abstract" xml:lang="en">Attempts at viral isolation were made in chicken kidney cell culture using kidney suspension as inoculum from 39 cases exhibiting typical gross and microscopic lesions of the nephritis‐nephrosis syndrome. Eleven viral isolates were recovered. Five isolates were identified as infectious bronchitis virus and one isolate as infectious bursal disease virus by neutralization test with anti‐IB serum and anti‐IBD serum, respectively. The remaining five isolates were identified as avian adenovirus in the agar gel precipitation test using anti‐CELO serum.</div>
<div type="abstract" xml:lang="de">Isolierung vom Virus der Infektiösen Bronchitis und der Infektiösen Bursitis sowie aviärer Adenoviren von Küken mit Nephritis‐Nephrose‐Syndrom Zur Virusisolierung wurden Kükennieren‐Zellkulturen mit Zellsuspensionen aus Nieren von 39 Küken beimpft, die für das Nephritis‐Nephrose‐Syndrom typische makroskopische und mikroskopische Veränderungen zeigten. Von 11 gewonnenen Virusisolaten konnten 5 als Infektiöse Bronchitisund 1 Isolat als Infektiöse Bursitis‐Viren im Neutralisationstest, die übrigen 5 Isolate als aviäre Adenoviren in der Agargelpräzipitation mittels entsprechender Antiseren nachgewiesen werden.</div>
<div type="abstract" xml:lang="es">Aislamiento de virus de la bronquitis infecciosa, bursitis infecciosa y adenovirus aviares a partir de pollitos con síndrome nefritis‐nefrosis Para poder aislar virus, se inyectaron cultivos celulares de riñones de pollitos con suspensiones celulares de riñones de 39 pollitos, los cuales presentaban las modificaciones macro y microscópicas típicas del síndrome nefritis‐nefrosis. Die 11 aislamientos de virus obtenidos, se pudieron identificar 5 como virus de la bronquitis infecciosa y 1 aislamiento como virus de la bursitis infecciosa en la prueba de neutralización, mientras que los 5 aislamientos restantes como adenovirus aviares en la precipitación en gelosa de agar mediante los antisueros correspondientes.</div>
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