Molecular screening by polymerase chain reaction detects panleukopenia virus DNA in formalin-fixed hearts from cats with idiopathic cardiomyopathy and myocarditis.
Identifieur interne : 001844 ( Main/Exploration ); précédent : 001843; suivant : 001845Molecular screening by polymerase chain reaction detects panleukopenia virus DNA in formalin-fixed hearts from cats with idiopathic cardiomyopathy and myocarditis.
Auteurs : K M Meurs [États-Unis] ; P R Fox ; A L Magnon ; S. Liu ; J A TowbinSource :
- Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology [ 1054-8807 ]
Descripteurs français
- KwdFr :
- ADN viral (analyse), Animaux, Cardiomyopathies (anatomopathologie), Cardiomyopathies (médecine vétérinaire), Chats, Coeur (virologie), Fixateurs, Formaldéhyde, Maladies des chats (virologie), Myocardite (anatomopathologie), Myocardite (médecine vétérinaire), Réaction de polymérisation en chaîne, Virus de la panleucopénie féline (génétique).
- MESH :
- analyse : ADN viral.
- anatomopathologie : Cardiomyopathies, Myocardite.
- génétique : Virus de la panleucopénie féline.
- médecine vétérinaire : Cardiomyopathies, Myocardite.
- virologie : Coeur, Maladies des chats.
- Animaux, Chats, Fixateurs, Formaldéhyde, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Viral.
- genetics : Feline Panleukopenia Virus.
- pathology : Cardiomyopathies, Myocarditis.
- veterinary : Cardiomyopathies, Myocarditis.
- virology : Cat Diseases, Heart.
- Animals, Cats, Fixatives, Formaldehyde, Polymerase Chain Reaction.
Abstract
Viral myocarditis has been suggested as an etiology for cardiomyopathy in several mammalian species. Myocarditis and idiopathic cardiomyopathy have been reported in the domestic cat, although a viral etiology has not been demonstrated. Because of the continuing interest in the potential relationship between viral myocarditis and cardiomyopathy, we evaluated hearts from cats with spontaneous, idiopathic cardiomyopathy for viral genomic material within myocytes by polymerase chain reaction, and for the presence of myocarditis by light microscopy. Thirty-one (31) formalin-fixed hearts from domestic cats who died of idiopathic cardiomyopathy were randomly selected from pathology archives. Seventeen (17) formalin-fixed hearts from healthy cats were similarly selected as normal controls. The polymerase chain reaction (PCR) was used to evaluate myocardial tissue for the presence of viral genome from feline panleukopenia virus, herpes virus, calici virus, and corona virus. Hearts were examined using light microscopy for histologic evidence of myocarditis according to the Dallas criteria. Panleukopenia virus was identified by PCR in 10 of 31 cats with cardiomyopathy but in none of the controls. Neither cardiomyopathic or control cats tested positive by PCR for herpes virus, calici virus, and corona virus. Myocarditis was detected by histologic examination in 18 of 31 cardiomyopathic cats and in none of 17 control cats. Myocarditis and or feline panleukopenia virus genome was detected in felines with idiopathic hypertrophic, dilated, and restrictive cardiomyopathy, suggesting a possible role of viral infection and inflammation in the pathogenesis of cardiomyopathy in this species.
DOI: 10.1016/s1054-8807(00)00031-4
PubMed: 10867362
Affiliations:
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Le document en format XML
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Liu</name></author><author><name sortKey="Towbin, J A" sort="Towbin, J A" uniqKey="Towbin J" first="J A" last="Towbin">J A Towbin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2000 Mar-Apr</MedlineDate></PubDate></date><idno type="RBID">pubmed:10867362</idno><idno type="pmid">10867362</idno><idno type="doi">10.1016/s1054-8807(00)00031-4</idno><idno type="wicri:Area/PubMed/Corpus">000726</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000726</idno><idno type="wicri:Area/PubMed/Curation">000726</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000726</idno><idno type="wicri:Area/PubMed/Checkpoint">000766</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000766</idno><idno type="wicri:Area/Ncbi/Merge">000005</idno><idno type="wicri:Area/Ncbi/Curation">000005</idno><idno type="wicri:Area/Ncbi/Checkpoint">000005</idno><idno type="wicri:doubleKey">1054-8807::Meurs K:molecular:screening:by</idno><idno type="wicri:Area/Main/Merge">001879</idno><idno type="wicri:Area/Main/Curation">001844</idno><idno type="wicri:Area/Main/Exploration">001844</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular screening by polymerase chain reaction detects panleukopenia virus DNA in formalin-fixed hearts from cats with idiopathic cardiomyopathy and myocarditis.</title><author><name sortKey="Meurs, K M" sort="Meurs, K M" uniqKey="Meurs K" first="K M" last="Meurs">K M Meurs</name><affiliation wicri:level="1"><nlm:affiliation>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH 43210, USA. meurs.1@osu.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH 43210</wicri:regionArea><wicri:noRegion>OH 43210</wicri:noRegion></affiliation></author><author><name sortKey="Fox, P R" sort="Fox, P R" uniqKey="Fox P" first="P R" last="Fox">P R Fox</name></author><author><name sortKey="Magnon, A L" sort="Magnon, A L" uniqKey="Magnon A" first="A L" last="Magnon">A L Magnon</name></author><author><name sortKey="Liu, S" sort="Liu, S" uniqKey="Liu S" first="S" last="Liu">S. Liu</name></author><author><name sortKey="Towbin, J A" sort="Towbin, J A" uniqKey="Towbin J" first="J A" last="Towbin">J A Towbin</name></author></analytic><series><title level="j">Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology</title><idno type="ISSN">1054-8807</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cardiomyopathies (pathology)</term><term>Cardiomyopathies (veterinary)</term><term>Cat Diseases (virology)</term><term>Cats</term><term>DNA, Viral (analysis)</term><term>Feline Panleukopenia Virus (genetics)</term><term>Fixatives</term><term>Formaldehyde</term><term>Heart (virology)</term><term>Myocarditis (pathology)</term><term>Myocarditis (veterinary)</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (analyse)</term><term>Animaux</term><term>Cardiomyopathies (anatomopathologie)</term><term>Cardiomyopathies (médecine vétérinaire)</term><term>Chats</term><term>Coeur (virologie)</term><term>Fixateurs</term><term>Formaldéhyde</term><term>Maladies des chats (virologie)</term><term>Myocardite (anatomopathologie)</term><term>Myocardite (médecine vétérinaire)</term><term>Réaction de polymérisation en chaîne</term><term>Virus de la panleucopénie féline (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN viral</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Cardiomyopathies</term><term>Myocardite</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Feline Panleukopenia Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de la panleucopénie féline</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Cardiomyopathies</term><term>Myocardite</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Cardiomyopathies</term><term>Myocarditis</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Cardiomyopathies</term><term>Myocarditis</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Coeur</term><term>Maladies des chats</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cat Diseases</term><term>Heart</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cats</term><term>Fixatives</term><term>Formaldehyde</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chats</term><term>Fixateurs</term><term>Formaldéhyde</term><term>Réaction de polymérisation en chaîne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Viral myocarditis has been suggested as an etiology for cardiomyopathy in several mammalian species. Myocarditis and idiopathic cardiomyopathy have been reported in the domestic cat, although a viral etiology has not been demonstrated. Because of the continuing interest in the potential relationship between viral myocarditis and cardiomyopathy, we evaluated hearts from cats with spontaneous, idiopathic cardiomyopathy for viral genomic material within myocytes by polymerase chain reaction, and for the presence of myocarditis by light microscopy. Thirty-one (31) formalin-fixed hearts from domestic cats who died of idiopathic cardiomyopathy were randomly selected from pathology archives. Seventeen (17) formalin-fixed hearts from healthy cats were similarly selected as normal controls. The polymerase chain reaction (PCR) was used to evaluate myocardial tissue for the presence of viral genome from feline panleukopenia virus, herpes virus, calici virus, and corona virus. Hearts were examined using light microscopy for histologic evidence of myocarditis according to the Dallas criteria. Panleukopenia virus was identified by PCR in 10 of 31 cats with cardiomyopathy but in none of the controls. Neither cardiomyopathic or control cats tested positive by PCR for herpes virus, calici virus, and corona virus. Myocarditis was detected by histologic examination in 18 of 31 cardiomyopathic cats and in none of 17 control cats. Myocarditis and or feline panleukopenia virus genome was detected in felines with idiopathic hypertrophic, dilated, and restrictive cardiomyopathy, suggesting a possible role of viral infection and inflammation in the pathogenesis of cardiomyopathy in this species.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Fox, P R" sort="Fox, P R" uniqKey="Fox P" first="P R" last="Fox">P R Fox</name><name sortKey="Liu, S" sort="Liu, S" uniqKey="Liu S" first="S" last="Liu">S. Liu</name><name sortKey="Magnon, A L" sort="Magnon, A L" uniqKey="Magnon A" first="A L" last="Magnon">A L Magnon</name><name sortKey="Towbin, J A" sort="Towbin, J A" uniqKey="Towbin J" first="J A" last="Towbin">J A Towbin</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Meurs, K M" sort="Meurs, K M" uniqKey="Meurs K" first="K M" last="Meurs">K M Meurs</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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