Coronavirus (JHM) Replication within the Retina: Analysis of Cell Tropism in Mouse Retinal Cell Cultures
Identifieur interne : 001356 ( Main/Exploration ); précédent : 001355; suivant : 001357Coronavirus (JHM) Replication within the Retina: Analysis of Cell Tropism in Mouse Retinal Cell Cultures
Auteurs : Yun Wang [États-Unis] ; Barbara Detrick [États-Unis] ; John J. Hooks [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1993.
Abstract
Abstract: The murine coronavirus, mouse hepatitis virus, JHM strain, induces a retinal degenerative disease in adult BALB/c mice. Coronavirus infections are highly species specific with virus exhibiting a strong tissue and cell specificity. In this report we evaluated the cellular basis of JHM virus retinal tropism. Retinal cultures and retinal pigment epithelial (RPE)-retinal mixed cell cultures were prepared from eyes obtained from Balb/c mice. The ability of JHM virus to infect and replicate in these retinal cultures was evaluated by light microscopy, immunofluorescent staining, electron microscopy, and virus isolation. Cytopathology was not observed and virus could not be detected in supernatant fluid in retinal cultures. However, low levels of infectious virus could be detected within the cells for the first 4 days. This observation suggested that cell-to-cell interactions may be critical since virus particles and virus antigens can be seen in vivo within the neural retina and the RPE. In contrast to the retinal cultures, retinal-RPE mixed cultures were supportive to JHM virus replication. Syncytial cytopathology was observed for the first 4 days and virus was isolated from supernatant fluids. By electron microscopy, virus was found intracellularly within vacuoles and extracellularly at the plasma membrane. After Day 4, a persistent virus infection was established in which cells produced virus for 5 weeks without cytopathic effects or cell death. Double-labeling immunofluorescent studies of retinal-RPE mixed cultures showed that the virus antigen was co-expressed with a Muller cell marker, glutamine synthetase. This cell is the most prominent glial element in the retina. These studies demonstrate that JHM virus is capable of establishing a persistent virus infection in mixed retinal (Muller)-RPE cell cultures. Moreover, these data suggest that cell-to-cell interactions influence the establishment of coronavirus infections in the retina.
Url:
DOI: 10.1006/viro.1993.1109
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000810
- to stream Istex, to step Curation: 000790
- to stream Istex, to step Checkpoint: 000521
- to stream Main, to step Merge: 001372
- to stream Main, to step Curation: 001356
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Coronavirus (JHM) Replication within the Retina: Analysis of Cell Tropism in Mouse Retinal Cell Cultures</title>
<author><name sortKey="Wang, Yun" sort="Wang, Yun" uniqKey="Wang Y" first="Yun" last="Wang">Yun Wang</name>
</author>
<author><name sortKey="Detrick, Barbara" sort="Detrick, Barbara" uniqKey="Detrick B" first="Barbara" last="Detrick">Barbara Detrick</name>
</author>
<author><name sortKey="Hooks, John J" sort="Hooks, John J" uniqKey="Hooks J" first="John J." last="Hooks">John J. Hooks</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:BFA735B8CB7B01D48FEA5D6D3E00A74E3B005232</idno>
<date when="1993" year="1993">1993</date>
<idno type="doi">10.1006/viro.1993.1109</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-91ZJBRGQ-W/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000810</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000810</idno>
<idno type="wicri:Area/Istex/Curation">000790</idno>
<idno type="wicri:Area/Istex/Checkpoint">000521</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000521</idno>
<idno type="wicri:doubleKey">0042-6822:1993:Wang Y:coronavirus:jhm:replication</idno>
<idno type="wicri:Area/Main/Merge">001372</idno>
<idno type="wicri:Area/Main/Curation">001356</idno>
<idno type="wicri:Area/Main/Exploration">001356</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Coronavirus (JHM) Replication within the Retina: Analysis of Cell Tropism in Mouse Retinal Cell Cultures</title>
<author><name sortKey="Wang, Yun" sort="Wang, Yun" uniqKey="Wang Y" first="Yun" last="Wang">Yun Wang</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">District de Columbia</region>
</placeName>
<wicri:cityArea>Immunology & Virology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892; and Department of Pathology, George Washington University Medical Center, Washington</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Detrick, Barbara" sort="Detrick, Barbara" uniqKey="Detrick B" first="Barbara" last="Detrick">Barbara Detrick</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">District de Columbia</region>
</placeName>
<wicri:cityArea>Immunology & Virology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892; and Department of Pathology, George Washington University Medical Center, Washington</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Hooks, John J" sort="Hooks, John J" uniqKey="Hooks J" first="John J." last="Hooks">John J. Hooks</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">District de Columbia</region>
</placeName>
<wicri:cityArea>Immunology & Virology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892; and Department of Pathology, George Washington University Medical Center, Washington</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Virology</title>
<title level="j" type="abbrev">YVIRO</title>
<idno type="ISSN">0042-6822</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1993">1993</date>
<biblScope unit="volume">193</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="124">124</biblScope>
<biblScope unit="page" to="137">137</biblScope>
</imprint>
<idno type="ISSN">0042-6822</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0042-6822</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: The murine coronavirus, mouse hepatitis virus, JHM strain, induces a retinal degenerative disease in adult BALB/c mice. Coronavirus infections are highly species specific with virus exhibiting a strong tissue and cell specificity. In this report we evaluated the cellular basis of JHM virus retinal tropism. Retinal cultures and retinal pigment epithelial (RPE)-retinal mixed cell cultures were prepared from eyes obtained from Balb/c mice. The ability of JHM virus to infect and replicate in these retinal cultures was evaluated by light microscopy, immunofluorescent staining, electron microscopy, and virus isolation. Cytopathology was not observed and virus could not be detected in supernatant fluid in retinal cultures. However, low levels of infectious virus could be detected within the cells for the first 4 days. This observation suggested that cell-to-cell interactions may be critical since virus particles and virus antigens can be seen in vivo within the neural retina and the RPE. In contrast to the retinal cultures, retinal-RPE mixed cultures were supportive to JHM virus replication. Syncytial cytopathology was observed for the first 4 days and virus was isolated from supernatant fluids. By electron microscopy, virus was found intracellularly within vacuoles and extracellularly at the plasma membrane. After Day 4, a persistent virus infection was established in which cells produced virus for 5 weeks without cytopathic effects or cell death. Double-labeling immunofluorescent studies of retinal-RPE mixed cultures showed that the virus antigen was co-expressed with a Muller cell marker, glutamine synthetase. This cell is the most prominent glial element in the retina. These studies demonstrate that JHM virus is capable of establishing a persistent virus infection in mixed retinal (Muller)-RPE cell cultures. Moreover, these data suggest that cell-to-cell interactions influence the establishment of coronavirus infections in the retina.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>District de Columbia</li>
</region>
</list>
<tree><country name="États-Unis"><region name="District de Columbia"><name sortKey="Wang, Yun" sort="Wang, Yun" uniqKey="Wang Y" first="Yun" last="Wang">Yun Wang</name>
</region>
<name sortKey="Detrick, Barbara" sort="Detrick, Barbara" uniqKey="Detrick B" first="Barbara" last="Detrick">Barbara Detrick</name>
<name sortKey="Hooks, John J" sort="Hooks, John J" uniqKey="Hooks J" first="John J." last="Hooks">John J. Hooks</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001356 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001356 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:BFA735B8CB7B01D48FEA5D6D3E00A74E3B005232 |texte= Coronavirus (JHM) Replication within the Retina: Analysis of Cell Tropism in Mouse Retinal Cell Cultures }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |