Antigenic differences between European and American isolates of porcine reproductive and respiratory syndrome virus (PRRSV) are encoded by the carboxyterminal portion of viral open reading frame 3
Identifieur interne : 001270 ( Main/Exploration ); précédent : 001269; suivant : 001271Antigenic differences between European and American isolates of porcine reproductive and respiratory syndrome virus (PRRSV) are encoded by the carboxyterminal portion of viral open reading frame 3
Auteurs : Jonathan B. Katz [États-Unis] ; Amy L. Shafer [États-Unis] ; Kenneth A. Eernisse [États-Unis] ; John G. Landgraf [États-Unis] ; Eric A. Nelson [États-Unis]Source :
- Veterinary Microbiology [ 0378-1135 ] ; 1995.
English descriptors
- Teeft :
- American prrs viruses, American swine, Amino, Amino acids, Antigenic, Antigenic differences, Antiserum, Baculovirus, Boehringer mannheim, Carboxyterminal portion, Cell cultures, Differentially reactive, Equine arteritis virus, Experimental reproduction, German sera, Glycosylation, Gnotobiotic, Gnotobiotic lelystad, Gnotobiotic swine, Hyperimmune rabbit serum, Immunoblot, Ipma, Katz, Lactate dehydrogenase, Lelystad, Lelystad virus, Microbiology, Molecular mass markers, Monoclonal antibodies, More reactive, Native protein, Nucleocapsid protein, Orfs, Porcine, Porcine epidemic abortion, Protein products, Prrs, Prrsv, Rabbits inoculated, Radioimmunoprecipitation assay, Reactive, Reading frame, Recombinant, Recombinant baculovirus, Recombinant fusion protein, Recombinant protein, Respiratory syndrome, Respiratory syndrome virus, Ripa, Serologic, Serologic analysis, Serologic differences, Simian hemorrhagic fever virus, Structural proteins, Swine, Swine infertility, Swine pratt, Syndrome, Veterinary microbiology, Viral, Viral antigen, Viral antigens, Virus, Wensvoort, Western immunoblot, Western immunoblotting.
Abstract
Abstract: Antigenic differences between European and American isolates of porcine reproductive and respiratory syndrome virus (PRRSV) were revealed by serologic analysis of a recombinant protein derived from PRRSV open reading frame 3 (ORF 3). The hydrophilic carboxyterminal 199 amino acids encoded by the ORF 3 of a European (Lelystad) isolate of PRRSV were expressed as a recombinant fusion protein (BP03-P) in a baculovirus gene expression system. Sera from gnotobiotic swine exposed to prototypic reference European and American isolates of PRRSV and sera from conventionally reared European and American swine convalescing from naturally acquired PRRSV infections were used to characterize the BP03-P protein. Sera from gnotobiotic and conventionally reared swine exposed to European isolates of PRRSV were significantly more reactive (P < 0.01) with BP03-P than were the corresponding American PRRSV antisera using the indirect immunoperoxidase monolayer assay (IPMA). Prototypic European, but not American, PRRSV antisera also recognized BP03-P using western immunoblotting and radioimmunoprecipitation assay (RIPA) procedures. However, gnotobiotically derived antiserum to an atypical American-origin PRRSV was reactive with BP03-P by both IPMA and western immunoblot. Despite a predicted potential for N-linked glycosylation, studies with tunicamycin and peptide-N-glycosidase F (PNGase F) indicated that BP03-P was not N-glycosylated in either insect cell cultures or Trichoplusia ni larvae infected with the recombinant baculovirus. Sera from rabbits inoculated with BP03-P failed to neutralize both the European (Lelystad) and American (ATCC VR-2332) reference isolates of PRRSV and did not react by IPMA with PRRSV-infected cell cultures. Taken together, the data suggest that the carboxyterminal portion of PRRSV ORF 3 encodes a non-neutralizing viral peptide that is partially responsible for the serologic differences noted between European and most American isolates of PRRSV.
Url:
DOI: 10.1016/0378-1135(94)00113-B
Affiliations:
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<term>More reactive</term>
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<front><div type="abstract" xml:lang="en">Abstract: Antigenic differences between European and American isolates of porcine reproductive and respiratory syndrome virus (PRRSV) were revealed by serologic analysis of a recombinant protein derived from PRRSV open reading frame 3 (ORF 3). The hydrophilic carboxyterminal 199 amino acids encoded by the ORF 3 of a European (Lelystad) isolate of PRRSV were expressed as a recombinant fusion protein (BP03-P) in a baculovirus gene expression system. Sera from gnotobiotic swine exposed to prototypic reference European and American isolates of PRRSV and sera from conventionally reared European and American swine convalescing from naturally acquired PRRSV infections were used to characterize the BP03-P protein. Sera from gnotobiotic and conventionally reared swine exposed to European isolates of PRRSV were significantly more reactive (P < 0.01) with BP03-P than were the corresponding American PRRSV antisera using the indirect immunoperoxidase monolayer assay (IPMA). Prototypic European, but not American, PRRSV antisera also recognized BP03-P using western immunoblotting and radioimmunoprecipitation assay (RIPA) procedures. However, gnotobiotically derived antiserum to an atypical American-origin PRRSV was reactive with BP03-P by both IPMA and western immunoblot. Despite a predicted potential for N-linked glycosylation, studies with tunicamycin and peptide-N-glycosidase F (PNGase F) indicated that BP03-P was not N-glycosylated in either insect cell cultures or Trichoplusia ni larvae infected with the recombinant baculovirus. Sera from rabbits inoculated with BP03-P failed to neutralize both the European (Lelystad) and American (ATCC VR-2332) reference isolates of PRRSV and did not react by IPMA with PRRSV-infected cell cultures. Taken together, the data suggest that the carboxyterminal portion of PRRSV ORF 3 encodes a non-neutralizing viral peptide that is partially responsible for the serologic differences noted between European and most American isolates of PRRSV.</div>
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