Direct structural evidence for formation of a stem-loop structure involved in ribosomal frameshifting in human immunodeficiency virus type 1
Identifieur interne : 001199 ( Main/Exploration ); précédent : 001198; suivant : 001200Direct structural evidence for formation of a stem-loop structure involved in ribosomal frameshifting in human immunodeficiency virus type 1
Auteurs : Hunseung Kang [Corée du Sud]Source :
- BBA - Gene Structure and Expression [ 0167-4781 ] ; 1998.
English descriptors
- Teeft :
- Biophysica acta, Enhancer, Enhancer sequence, Frameshift, Frameshifting, Frameshifting efficiency, Frameshifting sequence elements, Mrna, Nucleic acids, Nucleotide, Oligonucleotides, Pseudoknot, Pseudoknot structure, Ribosomal, Ribosomal frameshifting, Rnase, Secondary structure, Sequence element, Shift site, Simple structure, Slippery sequence, Sodium phosphate, Spacer, Spacer region, Spacer sequence.
Abstract
Abstract: Programmed ribosomal frameshifting in viral messenger RNA occurs in response to neighboring sequence elements consisting of: a frameshift site, a spacer, and a downstream enhancer sequence. In human immunodeficiency virus type 1 (HIV-1) mRNA, this sequence element has a potential to form either a stem-loop or a pseudoknot structure. Based on many mutational studies, the stem-loop structure has been proposed for the downstream enhancer region of the HIV-1 mRNA. This stimulatory stem-loop structure is separated from the shift site by a spacer of seven nucleotides. In contrast, a recent report has proposed an alternative model in which the bases in the spacer sequence form a pseudoknot structure as the downstream enhancer sequence [Du et al., Biochemistry 35 (1996) 4187–4198.]. Using UV melting and enzymatic mapping analyses, we have investigated the conformation of the sequence region involved in ribosomal frameshifting in HIV-1. Our S1, V1, and T1 endonuclease mappings, together with UV melting analysis, clearly indicate that this sequence element of the HIV-1 mRNA frameshift site forms a stem-loop structure, not a pseudoknot structure. This finding further supports the stem-loop structure proposed by many mutational studies for the downstream enhancer sequence of the HIV-1 mRNA.
Url:
DOI: 10.1016/S0167-4781(98)00004-9
Affiliations:
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Le document en format XML
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Ltd., 572, Ssangam-dong, Kwangsan-gu, Kwangju</wicri:regionArea><wicri:noRegion>Kwangju</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">BBA - Gene Structure and Expression</title><title level="j" type="abbrev">BBAEXP</title><idno type="ISSN">0167-4781</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">1397</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="73">73</biblScope><biblScope unit="page" to="78">78</biblScope></imprint><idno type="ISSN">0167-4781</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0167-4781</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Biophysica acta</term><term>Enhancer</term><term>Enhancer sequence</term><term>Frameshift</term><term>Frameshifting</term><term>Frameshifting efficiency</term><term>Frameshifting sequence elements</term><term>Mrna</term><term>Nucleic acids</term><term>Nucleotide</term><term>Oligonucleotides</term><term>Pseudoknot</term><term>Pseudoknot structure</term><term>Ribosomal</term><term>Ribosomal frameshifting</term><term>Rnase</term><term>Secondary structure</term><term>Sequence element</term><term>Shift site</term><term>Simple structure</term><term>Slippery sequence</term><term>Sodium phosphate</term><term>Spacer</term><term>Spacer region</term><term>Spacer sequence</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Programmed ribosomal frameshifting in viral messenger RNA occurs in response to neighboring sequence elements consisting of: a frameshift site, a spacer, and a downstream enhancer sequence. In human immunodeficiency virus type 1 (HIV-1) mRNA, this sequence element has a potential to form either a stem-loop or a pseudoknot structure. Based on many mutational studies, the stem-loop structure has been proposed for the downstream enhancer region of the HIV-1 mRNA. This stimulatory stem-loop structure is separated from the shift site by a spacer of seven nucleotides. In contrast, a recent report has proposed an alternative model in which the bases in the spacer sequence form a pseudoknot structure as the downstream enhancer sequence [Du et al., Biochemistry 35 (1996) 4187–4198.]. Using UV melting and enzymatic mapping analyses, we have investigated the conformation of the sequence region involved in ribosomal frameshifting in HIV-1. Our S1, V1, and T1 endonuclease mappings, together with UV melting analysis, clearly indicate that this sequence element of the HIV-1 mRNA frameshift site forms a stem-loop structure, not a pseudoknot structure. This finding further supports the stem-loop structure proposed by many mutational studies for the downstream enhancer sequence of the HIV-1 mRNA.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Kang, Hunseung" sort="Kang, Hunseung" uniqKey="Kang H" first="Hunseung" last="Kang">Hunseung Kang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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