Evaluation of Internal Reference Genes for Quantitative Expression Analysis by Real-Time PCR in Ovine Whole Blood
Identifieur interne : 000C54 ( Main/Exploration ); précédent : 000C53; suivant : 000C55Evaluation of Internal Reference Genes for Quantitative Expression Analysis by Real-Time PCR in Ovine Whole Blood
Auteurs : Simone Peletto ; Simone Bertuzzi ; Chiara Campanella ; Paola Modesto ; Maria Grazia Maniaci ; Claudio Bellino ; Dario Ariello ; Antonio Quasso ; Maria Caramelli ; Pier Luigi AcutisSource :
- International Journal of Molecular Sciences [ 1422-0067 ] ; 2011.
Abstract
The use of reference genes is commonly accepted as the most reliable approach to normalize qRT-PCR and to reduce possible errors in the quantification of gene expression. The most suitable reference genes in sheep have been identified for a restricted range of tissues, but no specific data on whole blood are available. The aim of this study was to identify a set of reference genes for normalizing qRT-PCR from ovine whole blood. We designed 11 PCR assays for commonly employed reference genes belonging to various functional classes and then determined their expression stability in whole blood samples from control and disease-stressed sheep.
Url:
DOI: 10.3390/ijms12117732
PubMed: 22174628
PubMed Central: 3233434
Affiliations:
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Evaluation of Internal Reference Genes for Quantitative Expression Analysis by Real-Time PCR in Ovine Whole Blood</title>
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<front><div type="abstract" xml:lang="en"><p>The use of reference genes is commonly accepted as the most reliable approach to normalize qRT-PCR and to reduce possible errors in the quantification of gene expression. The most suitable reference genes in sheep have been identified for a restricted range of tissues, but no specific data on whole blood are available. The aim of this study was to identify a set of reference genes for normalizing qRT-PCR from ovine whole blood. We designed 11 PCR assays for commonly employed reference genes belonging to various functional classes and then determined their expression stability in whole blood samples from control and disease-stressed sheep. <italic>SDHA</italic>
and <italic>YWHAZ</italic>
were considered the most suitable internal controls as they were stably expressed regardless of disease status according to both geNorm and NormFinder software; furthermore, geNorm indicated <italic>SDHA</italic>
/<italic>HPRT</italic>
, <italic>YWHAZ/GAPDH</italic>
and <italic>SDHA/YWHAZ</italic>
as the best reference gene combinations in control, disease-stressed and combined sheep groups, respectively. Our study provides a validated panel of optimal control genes which may be useful for the identification of genes differentially expressed by qRT-PCR in a readily accessible tissue, with potential for discovering new physiological and disease markers and as a tool to improve production traits (e.g., by identifying expression Quantitative Trait Loci). An additional outcome of the study is a set of intron-spanning primer sequences suitable for gene expression experiments employing SYBR Green chemistry on other ovine tissues and cells.</p>
</div>
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<tree><noCountry><name sortKey="Acutis, Pier Luigi" sort="Acutis, Pier Luigi" uniqKey="Acutis P" first="Pier Luigi" last="Acutis">Pier Luigi Acutis</name>
<name sortKey="Ariello, Dario" sort="Ariello, Dario" uniqKey="Ariello D" first="Dario" last="Ariello">Dario Ariello</name>
<name sortKey="Bellino, Claudio" sort="Bellino, Claudio" uniqKey="Bellino C" first="Claudio" last="Bellino">Claudio Bellino</name>
<name sortKey="Bertuzzi, Simone" sort="Bertuzzi, Simone" uniqKey="Bertuzzi S" first="Simone" last="Bertuzzi">Simone Bertuzzi</name>
<name sortKey="Campanella, Chiara" sort="Campanella, Chiara" uniqKey="Campanella C" first="Chiara" last="Campanella">Chiara Campanella</name>
<name sortKey="Caramelli, Maria" sort="Caramelli, Maria" uniqKey="Caramelli M" first="Maria" last="Caramelli">Maria Caramelli</name>
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