CovidV1, Main, Exploration, bibRecord, 000806

Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Identifieur interne : 000806 ( Main/Exploration ); précédent : 000805; suivant : 000807

Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Auteurs : Seung Gyu Yun ; Min Young Kim ; Jong Moon Choi ; Chang Kyu Lee ; Chae Seung Lim ; Yunjung Cho ; In Bum Suh

Source :

Journal of Clinical Laboratory Analysis [ 0887-8013 ] ; 2017.

RBID : PMC:5836940

Abstract

Background

Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT‐PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT‐PCR assays for the detection of respiratory viruses.

Methods

A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real‐Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence‐adjusted and bias‐adjusted kappa (PABAK) values was performed for comparisons among the three RV assays.

Results

Of the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%‐98%, 0.76‐0.86, and 0.93‐0.96 respectively. The performance of the three assays was very similar, with 94%‐100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results.

Conclusions

We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5836940

DOI: 10.1002/jcla.22230
PubMed: 28397965
PubMed Central: 5836940

Affiliations:

Le document en format XML

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 assays for detection of respiratory viruses: Anyplex <styled-content style="fixed-case">II RV</styled-content>
16, AdvanSure <styled-content style="fixed-case">RV</styled-content>
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<front><div type="abstract" xml:lang="en"><sec id="jcla22230-sec-0001"><title>Background</title>
<p>Due to its great sensitivity, the nucleic acid amplification test (<styled-content style="fixed-case">NAAT</styled-content>
) is widely used for detection of respiratory viruses (<styled-content style="fixed-case">RV</styled-content>
). However, few reports have described a direct comparison between multiplex <styled-content style="fixed-case">RT</styled-content>
‐<styled-content style="fixed-case">PCR</styled-content>
 assays for <styled-content style="fixed-case">RV</styled-content>
. The objective of this study was to perform a direct comparison of three multiplex <styled-content style="fixed-case">RT</styled-content>
‐<styled-content style="fixed-case">PCR</styled-content>
 assays for the detection of respiratory viruses.</p>
</sec>
<sec id="jcla22230-sec-0002"><title>Methods</title>
<p>A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial <styled-content style="fixed-case">RV</styled-content>
 assays: Seegene Anyplex <styled-content style="fixed-case">II RV</styled-content>
16 (<styled-content style="fixed-case">AP</styled-content>
), <styled-content style="fixed-case">LG</styled-content>
 AdvanSure <styled-content style="fixed-case">RV</styled-content>
 (<styled-content style="fixed-case">AD</styled-content>
), and Biosewoom Real‐Q <styled-content style="fixed-case">RV</styled-content>
 (<styled-content style="fixed-case">RQ</styled-content>
). The additional tests for the discrepant results were conducted by repeat <styled-content style="fixed-case">RV</styled-content>
 assay or monoplex <styled-content style="fixed-case">PCR</styled-content>
 coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence‐adjusted and bias‐adjusted kappa (<styled-content style="fixed-case">PABAK</styled-content>
) values was performed for comparisons among the three <styled-content style="fixed-case">RV</styled-content>
 assays.</p>
</sec>
<sec id="jcla22230-sec-0003"><title>Results</title>
<p>Of the 201 samples, <styled-content style="fixed-case">AP</styled-content>
,<styled-content style="fixed-case"> AD</styled-content>
, and <styled-content style="fixed-case">RQ</styled-content>
 detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and <styled-content style="fixed-case">PABAK</styled-content>
 values for the three assays ranged between 97%‐98%, 0.76‐0.86, and 0.93‐0.96 respectively. The performance of the three assays was very similar, with 94%‐100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that <styled-content style="fixed-case">AD</styled-content>
 assay had the highest rate of concordance with original results.</p>
</sec>
<sec id="jcla22230-sec-0004"><title>Conclusions</title>
<p>We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.</p>
</sec>
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Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Source :

Abstract

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