Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases
Identifieur interne : 000664 ( Main/Exploration ); précédent : 000663; suivant : 000665Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases
Auteurs : Fujiko Sunaga ; Shinobu Tsuchiaka ; Mai Kishimoto ; Hiroshi Aoki ; Mari Kakinoki ; Katsumasa Kure ; Hanako Okumura ; Maho Okumura ; Atsushi Okumura ; Makoto Nagai ; Tsutomu Omatsu ; Tetsuya MizutaniSource :
- The Journal of Veterinary Medical Science [ 0916-7250 ] ; 2019.
Abstract
The etiology of Porcine respiratory disease complex is complicated by infections with
multiple pathogens, and multiple infections increase the difficulty in identifying the
causal pathogen. In this present study, we developed a detection system of microbes from
porcine respiratory by using TaqMan real-time PCR (referred to as Dempo-PCR) to screen a
broad range of pathogens associated with porcine respiratory diseases in a single run. We
selected 17 porcine respiratory pathogens (
Url:
DOI: 10.1292/jvms.19-0063
PubMed: 31866601
PubMed Central: 7041981
Affiliations:
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associated with porcine respiratory diseases</title><author><name sortKey="Sunaga, Fujiko" sort="Sunaga, Fujiko" uniqKey="Sunaga F" first="Fujiko" last="Sunaga">Fujiko Sunaga</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Tsuchiaka, Shinobu" sort="Tsuchiaka, Shinobu" uniqKey="Tsuchiaka S" first="Shinobu" last="Tsuchiaka">Shinobu Tsuchiaka</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Kishimoto, Mai" sort="Kishimoto, Mai" uniqKey="Kishimoto M" first="Mai" last="Kishimoto">Mai Kishimoto</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Aoki, Hiroshi" sort="Aoki, Hiroshi" uniqKey="Aoki H" first="Hiroshi" last="Aoki">Hiroshi Aoki</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Kakinoki, Mari" sort="Kakinoki, Mari" uniqKey="Kakinoki M" first="Mari" last="Kakinoki">Mari Kakinoki</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Kure, Katsumasa" sort="Kure, Katsumasa" uniqKey="Kure K" first="Katsumasa" last="Kure">Katsumasa Kure</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Okumura, Hanako" sort="Okumura, Hanako" uniqKey="Okumura H" first="Hanako" last="Okumura">Hanako Okumura</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Okumura, Maho" sort="Okumura, Maho" uniqKey="Okumura M" first="Maho" last="Okumura">Maho Okumura</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Okumura, Atsushi" sort="Okumura, Atsushi" uniqKey="Okumura A" first="Atsushi" last="Okumura">Atsushi Okumura</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Nagai, Makoto" sort="Nagai, Makoto" uniqKey="Nagai M" first="Makoto" last="Nagai">Makoto Nagai</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Omatsu, Tsutomu" sort="Omatsu, Tsutomu" uniqKey="Omatsu T" first="Tsutomu" last="Omatsu">Tsutomu Omatsu</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31866601</idno><idno type="pmc">7041981</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041981</idno><idno type="RBID">PMC:7041981</idno><idno type="doi">10.1292/jvms.19-0063</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000642</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000642</idno><idno type="wicri:Area/Pmc/Curation">000642</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000642</idno><idno type="wicri:Area/Pmc/Checkpoint">000087</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000087</idno><idno type="wicri:Area/Ncbi/Merge">000803</idno><idno type="wicri:Area/Ncbi/Curation">000803</idno><idno type="wicri:Area/Ncbi/Checkpoint">000803</idno><idno type="wicri:doubleKey">0916-7250:2019:Sunaga F:development:of:a</idno><idno type="wicri:Area/Main/Merge">000666</idno><idno type="wicri:Area/Main/Curation">000664</idno><idno type="wicri:Area/Main/Exploration">000664</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development of a one-run real-time PCR detection system for pathogens
associated with porcine respiratory diseases</title><author><name sortKey="Sunaga, Fujiko" sort="Sunaga, Fujiko" uniqKey="Sunaga F" first="Fujiko" last="Sunaga">Fujiko Sunaga</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Tsuchiaka, Shinobu" sort="Tsuchiaka, Shinobu" uniqKey="Tsuchiaka S" first="Shinobu" last="Tsuchiaka">Shinobu Tsuchiaka</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Kishimoto, Mai" sort="Kishimoto, Mai" uniqKey="Kishimoto M" first="Mai" last="Kishimoto">Mai Kishimoto</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Aoki, Hiroshi" sort="Aoki, Hiroshi" uniqKey="Aoki H" first="Hiroshi" last="Aoki">Hiroshi Aoki</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Kakinoki, Mari" sort="Kakinoki, Mari" uniqKey="Kakinoki M" first="Mari" last="Kakinoki">Mari Kakinoki</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Kure, Katsumasa" sort="Kure, Katsumasa" uniqKey="Kure K" first="Katsumasa" last="Kure">Katsumasa Kure</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Okumura, Hanako" sort="Okumura, Hanako" uniqKey="Okumura H" first="Hanako" last="Okumura">Hanako Okumura</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Okumura, Maho" sort="Okumura, Maho" uniqKey="Okumura M" first="Maho" last="Okumura">Maho Okumura</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Okumura, Atsushi" sort="Okumura, Atsushi" uniqKey="Okumura A" first="Atsushi" last="Okumura">Atsushi Okumura</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Nagai, Makoto" sort="Nagai, Makoto" uniqKey="Nagai M" first="Makoto" last="Nagai">Makoto Nagai</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Omatsu, Tsutomu" sort="Omatsu, Tsutomu" uniqKey="Omatsu T" first="Tsutomu" last="Omatsu">Tsutomu Omatsu</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author></analytic><series><title level="j">The Journal of Veterinary Medical Science</title><idno type="ISSN">0916-7250</idno><idno type="eISSN">1347-7439</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The etiology of Porcine respiratory disease complex is complicated by infections with
multiple pathogens, and multiple infections increase the difficulty in identifying the
causal pathogen. In this present study, we developed a detection system of microbes from
porcine respiratory by using TaqMan real-time PCR (referred to as Dempo-PCR) to screen a
broad range of pathogens associated with porcine respiratory diseases in a single run. We
selected 17 porcine respiratory pathogens (<italic>Actinobacillus
pleuropneumoniae</italic>, <italic>Boldetella bronchiseptica</italic>,
<italic>Haemophilus parasuis</italic>, <italic>Pasteurella multocida</italic>,
<italic>Pasteurella multocid</italic>a toxin, <italic>Streptococcus suis</italic>,
<italic>Mycoplasma hyopneumoniae</italic>, <italic>Mycoplasma hyorhinis</italic>,
<italic>Mycoplasma hyosynovie</italic>, porcine circovirus 2, pseudorabies virus,
porcine cytomegalovirus, swine influenza A virus, porcine reproductive and respiratory
virus US strain, EU strain, porcine respiratory coronavirus and porcine hemagglutinating
encephalomyelitis virus) as detection targets and designed novel specific primer-probe
sets for seven of them. In sensitivity test by using standard curves from synthesized DNA,
all primer-probe sets showed high sensitivity. However, porcine reproductive and
respiratory virus is known to have a high frequency of genetic mutations, and the primer
and probe sequences will need to be checked at a considerable frequency when performing
Dempo-PCR from field samples. A total of 30 lung samples from swine showing respiratory
symptoms on six farms were tested by the Dempo-PCR to validate the assay’s clinical
performance. As the results, 12 pathogens (5 virus and 7 bacteria) were detected and
porcine reproductive and respiratory virus US strain, <italic>Mycoplasma
hyorhinis</italic>, <italic>Haemophilus parasuis</italic>, and porcine cytomegalovirus
were detected at high frequency. These results suggest that Dempo-PCR assay can be applied
as a screening system with wide detection targets.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Angen, O" uniqKey="Angen O">O. Angen</name></author><author><name sortKey="Jensen, J" uniqKey="Jensen J">J. Jensen</name></author><author><name sortKey="Lavritsen, D T" uniqKey="Lavritsen D">D. T. Lavritsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bonifait, L" uniqKey="Bonifait L">L. Bonifait</name></author><author><name sortKey="Veillette, M" uniqKey="Veillette M">M. Veillette</name></author><author><name sortKey="Letourneau, V" uniqKey="Letourneau V">V. Létourneau</name></author><author><name sortKey="Grenier, D" uniqKey="Grenier D">D. Grenier</name></author><author><name sortKey="Duchaine, C" uniqKey="Duchaine C">C. Duchaine</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Edington, N" uniqKey="Edington N">N. Edington</name></author><author><name sortKey="Wrathall, A E" uniqKey="Wrathall A">A. E. Wrathall</name></author><author><name sortKey="Done, J T" uniqKey="Done J">J. T. Done</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Giammarioli, M" uniqKey="Giammarioli M">M. Giammarioli</name></author><author><name sortKey="Pellegrini, C" uniqKey="Pellegrini C">C. Pellegrini</name></author><author><name sortKey="Casciari, C" uniqKey="Casciari C">C. Casciari</name></author><author><name sortKey="De Mia, G M" uniqKey="De Mia G">G. M. De Mia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grau Roma, L" uniqKey="Grau Roma L">L. Grau-Roma</name></author><author><name sortKey="Segales, J" uniqKey="Segales J">J. Segalés</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gu, W" uniqKey="Gu W">W. Gu</name></author><author><name sortKey="Zeng, N" uniqKey="Zeng N">N. Zeng</name></author><author><name sortKey="Zhou, L" uniqKey="Zhou L">L. Zhou</name></author><author><name sortKey="Ge, X" uniqKey="Ge X">X. Ge</name></author><author><name sortKey="Guo, X" uniqKey="Guo X">X. Guo</name></author><author><name sortKey="Yang, H" uniqKey="Yang H">H. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hansen, M S" uniqKey="Hansen M">M. S. Hansen</name></author><author><name sortKey="Pors, S E" uniqKey="Pors S">S. E. Pors</name></author><author><name sortKey="Jensen, H E" uniqKey="Jensen H">H. E. Jensen</name></author><author><name sortKey="Bille Hansen, V" uniqKey="Bille Hansen V">V. Bille-Hansen</name></author><author><name sortKey="Bisgaard, M" uniqKey="Bisgaard M">M. Bisgaard</name></author><author><name sortKey="Flachs, E M" uniqKey="Flachs E">E. M. Flachs</name></author><author><name sortKey="Nielsen, O L" uniqKey="Nielsen O">O. L. Nielsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harms, P A" uniqKey="Harms P">P. A. Harms</name></author><author><name sortKey="Halbur, P G" uniqKey="Halbur P">P. G. Halbur</name></author><author><name sortKey="Sorden, S D" uniqKey="Sorden S">S. D. Sorden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hernandez Garcia, J" uniqKey="Hernandez Garcia J">J. Hernandez-Garcia</name></author><author><name sortKey="Robben, N" uniqKey="Robben N">N. Robben</name></author><author><name sortKey="Magnee, D" uniqKey="Magnee D">D. Magnée</name></author><author><name sortKey="Eley, T" uniqKey="Eley T">T. Eley</name></author><author><name sortKey="Dennis, I" uniqKey="Dennis I">I. Dennis</name></author><author><name sortKey="Kayes, S M" uniqKey="Kayes S">S. M. Kayes</name></author><author><name sortKey="Thomson, J R" uniqKey="Thomson J">J. R. Thomson</name></author><author><name sortKey="Tucker, A W" uniqKey="Tucker A">A. W. Tucker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hirahara, T" uniqKey="Hirahara T">T. Hirahara</name></author><author><name sortKey="Yasuhara, H" uniqKey="Yasuhara H">H. Yasuhara</name></author><author><name sortKey="Matsui, O" uniqKey="Matsui O">O. Matsui</name></author><author><name sortKey="Izumida, A" uniqKey="Izumida A">A. Izumida</name></author><author><name sortKey="Yoshiki, K" uniqKey="Yoshiki K">K. Yoshiki</name></author><author><name sortKey="Fukuyama, S" uniqKey="Fukuyama S">S. Fukuyama</name></author><author><name sortKey="Kodama, K" uniqKey="Kodama K">K., Kodama</name></author><author><name sortKey="Nakai, M" uniqKey="Nakai M">M. Nakai</name></author><author><name sortKey="Sasaki, N" uniqKey="Sasaki N">N. Sasaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kishimoto, M" uniqKey="Kishimoto M">M. Kishimoto</name></author><author><name sortKey="Tsuchiaka, S" uniqKey="Tsuchiaka S">S. Tsuchiaka</name></author><author><name sortKey="Rahpaya, S S" uniqKey="Rahpaya S">S. S. Rahpaya</name></author><author><name sortKey="Hasebe, A" uniqKey="Hasebe A">A. Hasebe</name></author><author><name sortKey="Otsu, K" uniqKey="Otsu K">K. Otsu</name></author><author><name sortKey="Sugimura, S" uniqKey="Sugimura S">S. Sugimura</name></author><author><name sortKey="Kobayashi, S" uniqKey="Kobayashi S">S. Kobayashi</name></author><author><name sortKey="Komatsu, N" uniqKey="Komatsu N">N. Komatsu</name></author><author><name sortKey="Nagai, M" uniqKey="Nagai M">M. Nagai</name></author><author><name sortKey="Omatsu, T" uniqKey="Omatsu T">T. Omatsu</name></author><author><name sortKey="Naoi, Y" uniqKey="Naoi Y">Y. Naoi</name></author><author><name sortKey="Sano, K" uniqKey="Sano K">K. Sano</name></author><author><name sortKey="Okazaki Terashima, S" uniqKey="Okazaki Terashima S">S. Okazaki-Terashima</name></author><author><name sortKey="Oba, M" uniqKey="Oba M">M. Oba</name></author><author><name sortKey="Katayama, Y" uniqKey="Katayama Y">Y. Katayama</name></author><author><name sortKey="Sato, R" uniqKey="Sato R">R. Sato</name></author><author><name sortKey="Asai, T" uniqKey="Asai T">T. Asai</name></author><author><name sortKey="Mizutani, T" uniqKey="Mizutani T">T. Mizutani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kleiboeker, S B" uniqKey="Kleiboeker S">S. B. Kleiboeker</name></author><author><name sortKey="Schommer, S K" uniqKey="Schommer S">S. K. Schommer</name></author><author><name sortKey="Lee, S M" uniqKey="Lee S">S. M. Lee</name></author><author><name sortKey="Watkins, S" uniqKey="Watkins S">S. Watkins</name></author><author><name sortKey="Chittick, W" uniqKey="Chittick W">W. Chittick</name></author><author><name sortKey="Polson, D" uniqKey="Polson D">D. Polson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lin, K" uniqKey="Lin K">K. Lin</name></author><author><name sortKey="Wang, C" uniqKey="Wang C">C. Wang</name></author><author><name sortKey="Murtaugh, M P" uniqKey="Murtaugh M">M. P. Murtaugh</name></author><author><name sortKey="Ramamoorthy, S" uniqKey="Ramamoorthy S">S. Ramamoorthy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lorbach, J N" uniqKey="Lorbach J">J. N. Lorbach</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Nolting, J M" uniqKey="Nolting J">J. M. Nolting</name></author><author><name sortKey="Benjamin, M G" uniqKey="Benjamin M">M. G. Benjamin</name></author><author><name sortKey="Killian, M L" uniqKey="Killian M">M. L. Killian</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y. Zhang</name></author><author><name sortKey="Bowman, A S" uniqKey="Bowman A">A. S. Bowman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lung, O" uniqKey="Lung O">O. Lung</name></author><author><name sortKey="Ohene Adjei, S" uniqKey="Ohene Adjei S">S. Ohene-Adjei</name></author><author><name sortKey="Buchanan, C" uniqKey="Buchanan C">C. Buchanan</name></author><author><name sortKey="Joseph, T" uniqKey="Joseph T">T. Joseph</name></author><author><name sortKey="King, R" uniqKey="King R">R. King</name></author><author><name sortKey="Erickson, A" uniqKey="Erickson A">A. Erickson</name></author><author><name sortKey="Detmer, S" uniqKey="Detmer S">S. Detmer</name></author><author><name sortKey="Ambagala, A" uniqKey="Ambagala A">A. Ambagala</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ma, W" uniqKey="Ma W">W. Ma</name></author><author><name sortKey="Lager, K M" uniqKey="Lager K">K. M. Lager</name></author><author><name sortKey="Richt, J A" uniqKey="Richt J">J. A. Richt</name></author><author><name sortKey="Stoffregen, W C" uniqKey="Stoffregen W">W. C. Stoffregen</name></author><author><name sortKey="Zhou, F" uniqKey="Zhou F">F. Zhou</name></author><author><name sortKey="Yoon, K J" uniqKey="Yoon K">K. J. Yoon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maes, D" uniqKey="Maes D">D. Maes</name></author><author><name sortKey="Deluyker, H" uniqKey="Deluyker H">H. Deluyker</name></author><author><name sortKey="Verdonck, M" uniqKey="Verdonck M">M. Verdonck</name></author><author><name sortKey="Castryck, F" uniqKey="Castryck F">F. Castryck</name></author><author><name sortKey="Miry, C" uniqKey="Miry C">C. Miry</name></author><author><name sortKey="Vrijens, B" uniqKey="Vrijens B">B. Vrijens</name></author><author><name sortKey="De Kruif, A" uniqKey="De Kruif A">A. de Kruif</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marois, C" uniqKey="Marois C">C. Marois</name></author><author><name sortKey="Dory, D" uniqKey="Dory D">D. Dory</name></author><author><name sortKey="Fablet, C" uniqKey="Fablet C">C. Fablet</name></author><author><name sortKey="Madec, F" uniqKey="Madec F">F. Madec</name></author><author><name sortKey="Kobisch, M" uniqKey="Kobisch M">M. Kobisch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Masuda, T" uniqKey="Masuda T">T. Masuda</name></author><author><name sortKey="Tsuchiaka, S" uniqKey="Tsuchiaka S">S. Tsuchiaka</name></author><author><name sortKey="Ashiba, T" uniqKey="Ashiba T">T. Ashiba</name></author><author><name sortKey="Yamasato, H" uniqKey="Yamasato H">H. Yamasato</name></author><author><name sortKey="Fukunari, K" uniqKey="Fukunari K">K. Fukunari</name></author><author><name sortKey="Omatsu, T" uniqKey="Omatsu T">T. Omatsu</name></author><author><name sortKey="Furuya, T" uniqKey="Furuya T">T. Furuya</name></author><author><name sortKey="Shirai, J" uniqKey="Shirai J">J. Shirai</name></author><author><name sortKey="Mizutani, T" uniqKey="Mizutani T">T. Mizutani</name></author><author><name sortKey="Nagai, M" uniqKey="Nagai M">M. Nagai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Opriessnig, T" uniqKey="Opriessnig T">T. Opriessnig</name></author><author><name sortKey="Gimenez Lirola, L G" uniqKey="Gimenez Lirola L">L. G. Giménez-Lirola</name></author><author><name sortKey="Halbur, P G" uniqKey="Halbur P">P. G. Halbur</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rech, R R" uniqKey="Rech R">R. R. Rech</name></author><author><name sortKey="Gava, D" uniqKey="Gava D">D. Gava</name></author><author><name sortKey="Silva, M C" uniqKey="Silva M">M. C. Silva</name></author><author><name sortKey="Fernandes, L T" uniqKey="Fernandes L">L. T. Fernandes</name></author><author><name sortKey="Haach, V" uniqKey="Haach V">V. Haach</name></author><author><name sortKey="Ciacci Zanella, J R" uniqKey="Ciacci Zanella J">J. R. Ciacci-Zanella</name></author><author><name sortKey="Schaefer, R" uniqKey="Schaefer R">R. Schaefer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schmidt, C" uniqKey="Schmidt C">C. Schmidt</name></author><author><name sortKey="Cibulski, S P" uniqKey="Cibulski S">S. P. Cibulski</name></author><author><name sortKey="Andrade, C P" uniqKey="Andrade C">C. P. Andrade</name></author><author><name sortKey="Teixeira, T F" uniqKey="Teixeira T">T. F. Teixeira</name></author><author><name sortKey="Varela, A P M" uniqKey="Varela A">A. P. M. Varela</name></author><author><name sortKey="Scheffer, C M" uniqKey="Scheffer C">C. M. Scheffer</name></author><author><name sortKey="Franco, A C" uniqKey="Franco A">A. C. Franco</name></author><author><name sortKey="De Almeida, L L" uniqKey="De Almeida L">L. L. de Almeida</name></author><author><name sortKey="Roehe, P M" uniqKey="Roehe P">P. M. Roehe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tang, X" uniqKey="Tang X">X. Tang</name></author><author><name sortKey="Zhao, Z" uniqKey="Zhao Z">Z. Zhao</name></author><author><name sortKey="Hu, J" uniqKey="Hu J">J. Hu</name></author><author><name sortKey="Wu, B" uniqKey="Wu B">B. Wu</name></author><author><name sortKey="Cai, X" uniqKey="Cai X">X. Cai</name></author><author><name sortKey="He, Q" uniqKey="He Q">Q. He</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tizolova, A" uniqKey="Tizolova A">A. Tizolova</name></author><author><name sortKey="Brun, D" uniqKey="Brun D">D. Brun</name></author><author><name sortKey="Guiso, N" uniqKey="Guiso N">N. Guiso</name></author><author><name sortKey="Guillot, S" uniqKey="Guillot S">S. Guillot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tocqueville, V" uniqKey="Tocqueville V">V. Tocqueville</name></author><author><name sortKey="Ferre, S" uniqKey="Ferre S">S. Ferré</name></author><author><name sortKey="Nguyen, N H P" uniqKey="Nguyen N">N. H. P. Nguyen</name></author><author><name sortKey="Kempf, I" uniqKey="Kempf I">I. Kempf</name></author><author><name sortKey="Marois Crehan, C" uniqKey="Marois Crehan C">C. Marois-Créhan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tsuchiaka, S" uniqKey="Tsuchiaka S">S. Tsuchiaka</name></author><author><name sortKey="Masuda, T" uniqKey="Masuda T">T. Masuda</name></author><author><name sortKey="Sugimura, S" uniqKey="Sugimura S">S. Sugimura</name></author><author><name sortKey="Kobayashi, S" uniqKey="Kobayashi S">S. Kobayashi</name></author><author><name sortKey="Komatsu, N" uniqKey="Komatsu N">N. Komatsu</name></author><author><name sortKey="Nagai, M" uniqKey="Nagai M">M. Nagai</name></author><author><name sortKey="Omatsu, T" uniqKey="Omatsu T">T. Omatsu</name></author><author><name sortKey="Furuya, T" uniqKey="Furuya T">T. Furuya</name></author><author><name sortKey="Oba, M" uniqKey="Oba M">M. Oba</name></author><author><name sortKey="Katayama, Y" uniqKey="Katayama Y">Y. Katayama</name></author><author><name sortKey="Kanda, S" uniqKey="Kanda S">S. Kanda</name></author><author><name sortKey="Yokoyama, T" uniqKey="Yokoyama T">T. Yokoyama</name></author><author><name sortKey="Mizutani, T" uniqKey="Mizutani T">T. Mizutani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Turni, C" uniqKey="Turni C">C. Turni</name></author><author><name sortKey="Pyke, M" uniqKey="Pyke M">M. Pyke</name></author><author><name sortKey="Blackall, P J" uniqKey="Blackall P">P. J. Blackall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wernike, K" uniqKey="Wernike K">K. Wernike</name></author><author><name sortKey="Hoffmann, B" uniqKey="Hoffmann B">B. Hoffmann</name></author><author><name sortKey="Dauber, M" uniqKey="Dauber M">M. Dauber</name></author><author><name sortKey="Lange, E" uniqKey="Lange E">E. Lange</name></author><author><name sortKey="Schirrmeier, H" uniqKey="Schirrmeier H">H. Schirrmeier</name></author><author><name sortKey="Beer, M" uniqKey="Beer M">M. Beer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wernike, K" uniqKey="Wernike K">K. Wernike</name></author><author><name sortKey="Hoffmann, B" uniqKey="Hoffmann B">B. Hoffmann</name></author><author><name sortKey="Kalthoff, D" uniqKey="Kalthoff D">D. Kalthoff</name></author><author><name sortKey="Konig, P" uniqKey="Konig P">P. König</name></author><author><name sortKey="Beer, M" uniqKey="Beer M">M. Beer</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Aoki, Hiroshi" sort="Aoki, Hiroshi" uniqKey="Aoki H" first="Hiroshi" last="Aoki">Hiroshi Aoki</name><name sortKey="Kakinoki, Mari" sort="Kakinoki, Mari" uniqKey="Kakinoki M" first="Mari" last="Kakinoki">Mari Kakinoki</name><name sortKey="Kishimoto, Mai" sort="Kishimoto, Mai" uniqKey="Kishimoto M" first="Mai" last="Kishimoto">Mai Kishimoto</name><name sortKey="Kure, Katsumasa" sort="Kure, Katsumasa" uniqKey="Kure K" first="Katsumasa" last="Kure">Katsumasa Kure</name><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><name sortKey="Nagai, Makoto" sort="Nagai, Makoto" uniqKey="Nagai M" first="Makoto" last="Nagai">Makoto Nagai</name><name sortKey="Okumura, Atsushi" sort="Okumura, Atsushi" uniqKey="Okumura A" first="Atsushi" last="Okumura">Atsushi Okumura</name><name sortKey="Okumura, Hanako" sort="Okumura, Hanako" uniqKey="Okumura H" first="Hanako" last="Okumura">Hanako Okumura</name><name sortKey="Okumura, Maho" sort="Okumura, Maho" uniqKey="Okumura M" first="Maho" last="Okumura">Maho Okumura</name><name sortKey="Omatsu, Tsutomu" sort="Omatsu, Tsutomu" uniqKey="Omatsu T" first="Tsutomu" last="Omatsu">Tsutomu Omatsu</name><name sortKey="Sunaga, Fujiko" sort="Sunaga, Fujiko" uniqKey="Sunaga F" first="Fujiko" last="Sunaga">Fujiko Sunaga</name><name sortKey="Tsuchiaka, Shinobu" sort="Tsuchiaka, Shinobu" uniqKey="Tsuchiaka S" first="Shinobu" last="Tsuchiaka">Shinobu Tsuchiaka</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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