Kin recognition between medial Golgi enzymes in HeLa cells.
Identifieur interne : 001286 ( Main/Curation ); précédent : 001285; suivant : 001287Kin recognition between medial Golgi enzymes in HeLa cells.
Auteurs : T. Nilsson ; M H Hoe ; P. Slusarewicz ; C. Rabouille ; R. Watson ; F. Hunte ; G. Watzele ; E G Berger ; G. WarrenSource :
- The EMBO Journal [ 0261-4189 ] ; 1994.
Abstract
The medial Golgi enzymes, N-acetylglucosaminyltransferase I (NAGT I) and mannosidase II (Mann II), and the trans Golgi enzyme, beta-1,4-galactosyltransferase (GalT) were each retained in the endoplasmic reticulum (ER) by grafting on the cytoplasmic tail of the p33 invariant chain. Transient and stable expression of p33/NAGT I in HeLa cells caused relocation of endogenous Mann II to the ER and transient expression of p33/Mann II had a similar effect on endogenous NAGT I. Neither of these endogenous medial enzymes were affected by transient expression of p33/GalT. These data provide strong evidence for kin recognition between medial Golgi enzymes and suggest a role for them in the organization of the Golgi stack.
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PubMed: 8313901
PubMed Central: 394845
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