Response of Rat Tracheal Epithelium to Ozone and Oxygen Exposure in Vitro
Identifieur interne : 000685 ( Istex/Curation ); précédent : 000684; suivant : 000686Response of Rat Tracheal Epithelium to Ozone and Oxygen Exposure in Vitro
Auteurs : Kristen J. Nikula ; Dennis W. Wilson [États-Unis]Source :
- Toxicological Sciences [ 1096-6080 ] ; 1990.
Abstract
Response of Rat Tracheal Epithelium to ozone and Oxygen Exposure in Vitro. NIKULA, K. J., AND WILSON, D. W. (1990). Fundam. Appl. Toxicol. 15, 121–131. Although ozone-induced epithelial injury in vivo has been morphologically characterized, effects of gaseous oxidants on respiratory epithelium in organ culture, where tissue organization is maintained but systemic influences are eliminated, have not been thoroughly investigated. In this study, we exposed tracheal organ cultures from rats to 95% oxygen and 1 ppm ozone, alone and in combination, to determine (1) whether epithelial responses to ozone similar to those observed in vivo occur in airways separated from systemic physiologic, secretory, and inflammatory reactions; (2) whether concentrations of oxygen sufficient to potentially cause oxidant injury result in morphologic epithelial alterations similar to those that occur in ozone toxicity; and (3) if the combined oxidant insult of oxygen and ozone results in more severe damage to the tracheal epithelium than occurs with ozone in air. Tracheal organ cultures were exposed to filtered air and 5% carbon dioxide; filtered air, 5% carbon dioxide, and 1 ppm ozone; 95% oxygen and 5% carbon dioxide; or 95% oxygen, 5% carbon dioxide, and 1 ppm ozone for 96 hr. Light- and quantitative electron-microscopic evaluation showed that epithelia exposed to 1 ppm ozone in air exhibited loss of ciliated cells and ciliated cell damage. The epithelia exposed to 95% oxygen and 5% carbon dioxide were pseudostratified, columnar, ciliated, and hyperplastic. Epithelia exposed to 95% oxygen plus 1 ppm ozone were stratified and nonciliated or very sparsely ciliated. The predominant cell types in epithelia exposed to oxygen plus ozone were serous cells and metaplastic cells, and focal aggregates of adherent necrotic cells were present. We conclude that there was a synergism between oxygen and ozone exposure leading to enhanced epithelial injury and metaplasia.
Url:
DOI: 10.1093/toxsci/15.1.121
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000705
Links to Exploration step
ISTEX:F70AFEBDB9A7D839596D1293160F7710D1844A44Curation
No country items
Kristen J. Nikula<affiliation><mods:affiliation>Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute P.O Box 5890, Albuquerque, New Mexico 87l85</mods:affiliation>
<wicri:noCountry code="subField">87l85</wicri:noCountry>
</affiliation>
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Response of Rat Tracheal Epithelium to Ozone and Oxygen Exposure in Vitro</title>
<author><name sortKey="Nikula, Kristen J" sort="Nikula, Kristen J" uniqKey="Nikula K" first="Kristen J." last="Nikula">Kristen J. Nikula</name>
<affiliation><mods:affiliation>Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute P.O Box 5890, Albuquerque, New Mexico 87l85</mods:affiliation>
<wicri:noCountry code="subField">87l85</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wilson, Dennis W" sort="Wilson, Dennis W" uniqKey="Wilson D" first="Dennis W." last="Wilson">Dennis W. Wilson</name>
<affiliation wicri:level="2"><mods:affiliation>Department of Pathology, School of Veterinary Medicine, University of California Davis, California 95616</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Department of Pathology, School of Veterinary Medicine, University of California Davis</wicri:cityArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:F70AFEBDB9A7D839596D1293160F7710D1844A44</idno>
<date when="1990" year="1990">1990</date>
<idno type="doi">10.1093/toxsci/15.1.121</idno>
<idno type="url">https://api.istex.fr/ark:/67375/HXZ-387GJ4MJ-K/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000705</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000705</idno>
<idno type="wicri:Area/Istex/Curation">000685</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Response of Rat Tracheal Epithelium to Ozone and Oxygen Exposure <hi rend="italic">in Vitro</hi>
</title>
<author><name sortKey="Nikula, Kristen J" sort="Nikula, Kristen J" uniqKey="Nikula K" first="Kristen J." last="Nikula">Kristen J. Nikula</name>
<affiliation><mods:affiliation>Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute P.O Box 5890, Albuquerque, New Mexico 87l85</mods:affiliation>
<wicri:noCountry code="subField">87l85</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wilson, Dennis W" sort="Wilson, Dennis W" uniqKey="Wilson D" first="Dennis W." last="Wilson">Dennis W. Wilson</name>
<affiliation wicri:level="2"><mods:affiliation>Department of Pathology, School of Veterinary Medicine, University of California Davis, California 95616</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Department of Pathology, School of Veterinary Medicine, University of California Davis</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Toxicological Sciences</title>
<idno type="eISSN">1096-0929</idno>
<idno type="ISSN">1096-6080</idno>
<imprint><publisher>Oxford University Press</publisher>
<date when="1990-07">1990</date>
<biblScope unit="vol">15</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="121">121</biblScope>
<biblScope unit="page" to="131">131</biblScope>
</imprint>
<idno type="ISSN">1096-6080</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1096-6080</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">Response of Rat Tracheal Epithelium to ozone and Oxygen Exposure in Vitro. NIKULA, K. J., AND WILSON, D. W. (1990). Fundam. Appl. Toxicol. 15, 121–131. Although ozone-induced epithelial injury in vivo has been morphologically characterized, effects of gaseous oxidants on respiratory epithelium in organ culture, where tissue organization is maintained but systemic influences are eliminated, have not been thoroughly investigated. In this study, we exposed tracheal organ cultures from rats to 95% oxygen and 1 ppm ozone, alone and in combination, to determine (1) whether epithelial responses to ozone similar to those observed in vivo occur in airways separated from systemic physiologic, secretory, and inflammatory reactions; (2) whether concentrations of oxygen sufficient to potentially cause oxidant injury result in morphologic epithelial alterations similar to those that occur in ozone toxicity; and (3) if the combined oxidant insult of oxygen and ozone results in more severe damage to the tracheal epithelium than occurs with ozone in air. Tracheal organ cultures were exposed to filtered air and 5% carbon dioxide; filtered air, 5% carbon dioxide, and 1 ppm ozone; 95% oxygen and 5% carbon dioxide; or 95% oxygen, 5% carbon dioxide, and 1 ppm ozone for 96 hr. Light- and quantitative electron-microscopic evaluation showed that epithelia exposed to 1 ppm ozone in air exhibited loss of ciliated cells and ciliated cell damage. The epithelia exposed to 95% oxygen and 5% carbon dioxide were pseudostratified, columnar, ciliated, and hyperplastic. Epithelia exposed to 95% oxygen plus 1 ppm ozone were stratified and nonciliated or very sparsely ciliated. The predominant cell types in epithelia exposed to oxygen plus ozone were serous cells and metaplastic cells, and focal aggregates of adherent necrotic cells were present. We conclude that there was a synergism between oxygen and ozone exposure leading to enhanced epithelial injury and metaplasia.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000685 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000685 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:F70AFEBDB9A7D839596D1293160F7710D1844A44 |texte= Response of Rat Tracheal Epithelium to Ozone and Oxygen Exposure in Vitro }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |