Mouse Hepatitis Virus Strain JHM Infects a Human Hepatocellular Carcinoma Cell Line
Identifieur interne : 000674 ( Istex/Curation ); précédent : 000673; suivant : 000675Mouse Hepatitis Virus Strain JHM Infects a Human Hepatocellular Carcinoma Cell Line
Auteurs : Peter J. Koetters ; Loubna Hassanieh ; Stephen A. Stohlman ; Thomas Gallagher ; Michael M. C. LaiSource :
- Virology [ 0042-6822 ] ; 1999.
English descriptors
- Teeft :
- Adoptive transfer, Assay, Baric, Biliary glycoprotein, Biol, Block infection, Cabirac, Carcinoma, Cause fusion, Cell fusion, Cell lines, Cell types, Chen, Coronavirus, Coronaviruses, Cultured cells, Demyelinating disease, Demyelination, Different animal species, Double staining, First hour, Fusion activity, Genomic structures, Glycoprotein, Growth curves, Hcea, Hensley, Hepatitis, Hepatocellular, Hepatotropic strain, Hepg2, Hepg2 cells, Host range, Howard hughes, Human cell line, Human cell lines, Human cells, Human coronavirus, Human disease, Human hepatocellular carcinoma cell line, Infection, Infects, Initial virus infection, Interspecies transfer, Lower virus titer, Mbgp, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Mouse, Mouse cells, Mouse hepatitis virus, Mouse hepatitis virus entry, Mouse hepatitis virus receptors, Multiple sclerosis, Multiple sclerosis patients, Murine, Murine coronavirus spike protein, Murine coronaviruses, Neurotropic strain, Neutralizing antibody, Newborn calf serum, Other strains, Parental strains, Plaque, Plaque assay, Plaque formation, Plaque size, Postinfection, Primate, Protein expression, Protein production, Protein staining, Rabbit polyclonal antibody, Receptor, Receptor mbgp, Receptor specificity, Receptor utilization, Recombinant, Recombinant strains, Recombinant viruses, Replication, Room temperature, Same extent, Same mechanism, Sclerosis, Soluble form, Spike protein, Stohlman, Structural proteins, Syncytia formation, Syncytium, Titer, Transfected, Tryptone phosphate broth, Viral, Virol, Virology, Virus, Virus production, Virus replication, Virus strains, Virus titers, Yokomori.
Abstract
Abstract: Mouse hepatitis virus (MHV) strain JHM is a coronavirus that causes encephalitis and demyelination in susceptible rodents. The known receptors for MHV are all members of the carcinoembryonic antigen family. Although human forms of the MHV receptor can function as MHV receptors in some assays, no human cell line has been identified that can support wild-type MHV infection. Here we describe the infection of a human hepatocellular carcinoma cell line, HuH-7, with MHV. HuH-7 cells were susceptible to strains JHM-DL and JHM-DS, yielding virus titers nearly identical to those seen in mouse DBT cells. In contrast, HuH-7 cells were only marginally susceptible or completely resistant to infection by other MHV strains, including A59. JHM produced a strong cytopathic effect in HuH-7 cells with the formation of round plaques. Studies of various recombinant viruses between JHM and A59 strains suggested that the ability of JHM to infect HuH-7 cells was determined by multiple viral genetic elements. Blocking the viral spike (S) protein with a neutralizing antibody or a soluble form of the MHV receptor inhibited infection of HuH-7 cells, suggesting that infection is mediated through the S protein. Transfection with the prototype mouse receptor, biliary glycoprotein, rendered HuH-7 cells susceptible to infection by other MHV strains as well, suggesting that JHM uses a receptor distinct from the classical MHV receptor to infect HuH-7 cells. Possible implications for human disease are discussed.
Url:
DOI: 10.1006/viro.1999.9984
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Peter J. Koetters<affiliation><mods:affiliation>Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, California, 90033</mods:affiliation>
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<affiliation><mods:affiliation>Loyola University Medical Center, Maywood, Illinois, 60153</mods:affiliation>
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<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adoptive transfer</term>
<term>Assay</term>
<term>Baric</term>
<term>Biliary glycoprotein</term>
<term>Biol</term>
<term>Block infection</term>
<term>Cabirac</term>
<term>Carcinoma</term>
<term>Cause fusion</term>
<term>Cell fusion</term>
<term>Cell lines</term>
<term>Cell types</term>
<term>Chen</term>
<term>Coronavirus</term>
<term>Coronaviruses</term>
<term>Cultured cells</term>
<term>Demyelinating disease</term>
<term>Demyelination</term>
<term>Different animal species</term>
<term>Double staining</term>
<term>First hour</term>
<term>Fusion activity</term>
<term>Genomic structures</term>
<term>Glycoprotein</term>
<term>Growth curves</term>
<term>Hcea</term>
<term>Hensley</term>
<term>Hepatitis</term>
<term>Hepatocellular</term>
<term>Hepatotropic strain</term>
<term>Hepg2</term>
<term>Hepg2 cells</term>
<term>Host range</term>
<term>Howard hughes</term>
<term>Human cell line</term>
<term>Human cell lines</term>
<term>Human cells</term>
<term>Human coronavirus</term>
<term>Human disease</term>
<term>Human hepatocellular carcinoma cell line</term>
<term>Infection</term>
<term>Infects</term>
<term>Initial virus infection</term>
<term>Interspecies transfer</term>
<term>Lower virus titer</term>
<term>Mbgp</term>
<term>Monoclonal</term>
<term>Monoclonal antibodies</term>
<term>Monoclonal antibody</term>
<term>Mouse</term>
<term>Mouse cells</term>
<term>Mouse hepatitis virus</term>
<term>Mouse hepatitis virus entry</term>
<term>Mouse hepatitis virus receptors</term>
<term>Multiple sclerosis</term>
<term>Multiple sclerosis patients</term>
<term>Murine</term>
<term>Murine coronavirus spike protein</term>
<term>Murine coronaviruses</term>
<term>Neurotropic strain</term>
<term>Neutralizing antibody</term>
<term>Newborn calf serum</term>
<term>Other strains</term>
<term>Parental strains</term>
<term>Plaque</term>
<term>Plaque assay</term>
<term>Plaque formation</term>
<term>Plaque size</term>
<term>Postinfection</term>
<term>Primate</term>
<term>Protein expression</term>
<term>Protein production</term>
<term>Protein staining</term>
<term>Rabbit polyclonal antibody</term>
<term>Receptor</term>
<term>Receptor mbgp</term>
<term>Receptor specificity</term>
<term>Receptor utilization</term>
<term>Recombinant</term>
<term>Recombinant strains</term>
<term>Recombinant viruses</term>
<term>Replication</term>
<term>Room temperature</term>
<term>Same extent</term>
<term>Same mechanism</term>
<term>Sclerosis</term>
<term>Soluble form</term>
<term>Spike protein</term>
<term>Stohlman</term>
<term>Structural proteins</term>
<term>Syncytia formation</term>
<term>Syncytium</term>
<term>Titer</term>
<term>Transfected</term>
<term>Tryptone phosphate broth</term>
<term>Viral</term>
<term>Virol</term>
<term>Virology</term>
<term>Virus</term>
<term>Virus production</term>
<term>Virus replication</term>
<term>Virus strains</term>
<term>Virus titers</term>
<term>Yokomori</term>
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<front><div type="abstract" xml:lang="en">Abstract: Mouse hepatitis virus (MHV) strain JHM is a coronavirus that causes encephalitis and demyelination in susceptible rodents. The known receptors for MHV are all members of the carcinoembryonic antigen family. Although human forms of the MHV receptor can function as MHV receptors in some assays, no human cell line has been identified that can support wild-type MHV infection. Here we describe the infection of a human hepatocellular carcinoma cell line, HuH-7, with MHV. HuH-7 cells were susceptible to strains JHM-DL and JHM-DS, yielding virus titers nearly identical to those seen in mouse DBT cells. In contrast, HuH-7 cells were only marginally susceptible or completely resistant to infection by other MHV strains, including A59. JHM produced a strong cytopathic effect in HuH-7 cells with the formation of round plaques. Studies of various recombinant viruses between JHM and A59 strains suggested that the ability of JHM to infect HuH-7 cells was determined by multiple viral genetic elements. Blocking the viral spike (S) protein with a neutralizing antibody or a soluble form of the MHV receptor inhibited infection of HuH-7 cells, suggesting that infection is mediated through the S protein. Transfection with the prototype mouse receptor, biliary glycoprotein, rendered HuH-7 cells susceptible to infection by other MHV strains as well, suggesting that JHM uses a receptor distinct from the classical MHV receptor to infect HuH-7 cells. Possible implications for human disease are discussed.</div>
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