Sequence Analysis of the Porcine Epidemic Diarrhea Virus Genome between the Nucleocapsid and Spike Protein Genes Reveals a Polymorphic ORF
Identifieur interne : 000222 ( Istex/Curation ); précédent : 000221; suivant : 000223Sequence Analysis of the Porcine Epidemic Diarrhea Virus Genome between the Nucleocapsid and Spike Protein Genes Reveals a Polymorphic ORF
Auteurs : Mariela Duarte [Suisse] ; Kurt Tobler [Suisse] ; Anne Bridgen [Suisse] ; Denis Rasschaert [Suisse] ; Mathias Ackermann [Suisse] ; Hubert Laude [Suisse]Source :
- Virology [ 0042-6822 ] ; 1994.
Abstract
Abstract: In order to investigate the genome organization of the porcine epidemic diarrhea virus (PEDV) further, cDNA clones covering the region between the nucleocapsid and the spike (S) protein genes were independently constructed and sequenced for the two virulent isolates Br1/87 and CV777. Of the three major ORFs identified, two were found to encode the major and minor coronavirus membrane proteins M and sM. A potentially single ORF, designated ORF3 according to the pattern of the viral subgenomic mRNAs, could be identified between the S and sM genes. A striking variability, essentially generated by short deletions clustered in a few loci, was observed in the ORF3 of both isolates. The largest predicted polypeptide of 223 amino acids showed homology with polypeptides potentially encoded by other members of the same genetic subset, including two shorter polypeptides of human respiratory virus HCV 229E and one of transmissible gastroenteritis virus TGEV. This homology suggests that the two HCV ORFs may have originated from a single precursor. The function of these polypeptides is not known, but the predicted products of the PEDV ORF3 and related ORFs share features suggestive of a membrane-associated protein.
Url:
DOI: 10.1006/viro.1994.1058
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000242
Links to Exploration step
ISTEX:8C06C0CA1EBB82D2F961DCE44A0C87C0485BA714Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sequence Analysis of the Porcine Epidemic Diarrhea Virus Genome between the Nucleocapsid and Spike Protein Genes Reveals a Polymorphic ORF</title><author><name sortKey="Duarte, Mariela" sort="Duarte, Mariela" uniqKey="Duarte M" first="Mariela" last="Duarte">Mariela Duarte</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author><author><name sortKey="Tobler, Kurt" sort="Tobler, Kurt" uniqKey="Tobler K" first="Kurt" last="Tobler">Kurt Tobler</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author><author><name sortKey="Bridgen, Anne" sort="Bridgen, Anne" uniqKey="Bridgen A" first="Anne" last="Bridgen">Anne Bridgen</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author><author><name sortKey="Rasschaert, Denis" sort="Rasschaert, Denis" uniqKey="Rasschaert D" first="Denis" last="Rasschaert">Denis Rasschaert</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author><author><name sortKey="Ackermann, Mathias" sort="Ackermann, Mathias" uniqKey="Ackermann M" first="Mathias" last="Ackermann">Mathias Ackermann</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author><author><name sortKey="Laude, Hubert" sort="Laude, Hubert" uniqKey="Laude H" first="Hubert" last="Laude">Hubert Laude</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8C06C0CA1EBB82D2F961DCE44A0C87C0485BA714</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1006/viro.1994.1058</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-5V3F98Q3-Q/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000242</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000242</idno><idno type="wicri:Area/Istex/Curation">000222</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Sequence Analysis of the Porcine Epidemic Diarrhea Virus Genome between the Nucleocapsid and Spike Protein Genes Reveals a Polymorphic ORF</title><author><name sortKey="Duarte, Mariela" sort="Duarte, Mariela" uniqKey="Duarte M" first="Mariela" last="Duarte">Mariela Duarte</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author><author><name sortKey="Tobler, Kurt" sort="Tobler, Kurt" uniqKey="Tobler K" first="Kurt" last="Tobler">Kurt Tobler</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author><author><name sortKey="Bridgen, Anne" sort="Bridgen, Anne" uniqKey="Bridgen A" first="Anne" last="Bridgen">Anne Bridgen</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author><author><name sortKey="Rasschaert, Denis" sort="Rasschaert, Denis" uniqKey="Rasschaert D" first="Denis" last="Rasschaert">Denis Rasschaert</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author><author><name sortKey="Ackermann, Mathias" sort="Ackermann, Mathias" uniqKey="Ackermann M" first="Mathias" last="Ackermann">Mathias Ackermann</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author><author><name sortKey="Laude, Hubert" sort="Laude, Hubert" uniqKey="Laude H" first="Hubert" last="Laude">Hubert Laude</name><affiliation wicri:level="1"><mods:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, 78350 Jouy-en-Josas, France; and Institut für Virologie, Vet. Med. Fak. der Universität Zürich, Winterthurerstrasse 266a, CH-8057 Zürich</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">198</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="466">466</biblScope><biblScope unit="page" to="476">476</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: In order to investigate the genome organization of the porcine epidemic diarrhea virus (PEDV) further, cDNA clones covering the region between the nucleocapsid and the spike (S) protein genes were independently constructed and sequenced for the two virulent isolates Br1/87 and CV777. Of the three major ORFs identified, two were found to encode the major and minor coronavirus membrane proteins M and sM. A potentially single ORF, designated ORF3 according to the pattern of the viral subgenomic mRNAs, could be identified between the S and sM genes. A striking variability, essentially generated by short deletions clustered in a few loci, was observed in the ORF3 of both isolates. The largest predicted polypeptide of 223 amino acids showed homology with polypeptides potentially encoded by other members of the same genetic subset, including two shorter polypeptides of human respiratory virus HCV 229E and one of transmissible gastroenteritis virus TGEV. This homology suggests that the two HCV ORFs may have originated from a single precursor. The function of these polypeptides is not known, but the predicted products of the PEDV ORF3 and related ORFs share features suggestive of a membrane-associated protein.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000222 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000222 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:8C06C0CA1EBB82D2F961DCE44A0C87C0485BA714
|texte= Sequence Analysis of the Porcine Epidemic Diarrhea Virus Genome between the Nucleocapsid and Spike Protein Genes Reveals a Polymorphic ORF
}}
| This area was generated with Dilib version V0.6.33. |  |