Moesin: A Cell Membrane Protein Linked with Susceptibility to Measles Virus Infection
Identifieur interne : 000826 ( Istex/Corpus ); précédent : 000825; suivant : 000827Moesin: A Cell Membrane Protein Linked with Susceptibility to Measles Virus Infection
Auteurs : Lee M. Dunster ; Jürgen Schneider-Schaulies ; Sieglinde Lüffler ; Wolfgang Lankes ; Reinhard Schwartz-Albiez ; Friedrich Lottspeich ; Volker Ter MeulenSource :
- Virology [ 0042-6822 ] ; 1994.
Abstract
Abstract: Measles virus is a highly contagious virus causing acute and persistent diseases in man, the receptor of which is still not well characterized. We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from 125I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. Our data suggest that moesin is acting as a receptor for measles virus.
Url:
DOI: 10.1006/viro.1994.1029
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Dunster</name><affiliation><mods:affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</mods:affiliation></affiliation></author><author><name sortKey="Schneider Schaulies, Jurgen" sort="Schneider Schaulies, Jurgen" uniqKey="Schneider Schaulies J" first="Jürgen" last="Schneider-Schaulies">Jürgen Schneider-Schaulies</name><affiliation><mods:affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</mods:affiliation></affiliation></author><author><name sortKey="Luffler, Sieglinde" sort="Luffler, Sieglinde" uniqKey="Luffler S" first="Sieglinde" last="Lüffler">Sieglinde Lüffler</name><affiliation><mods:affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</mods:affiliation></affiliation></author><author><name sortKey="Lankes, Wolfgang" sort="Lankes, Wolfgang" uniqKey="Lankes W" first="Wolfgang" last="Lankes">Wolfgang Lankes</name><affiliation><mods:affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</mods:affiliation></affiliation></author><author><name sortKey="Schwartz Albiez, Reinhard" sort="Schwartz Albiez, Reinhard" uniqKey="Schwartz Albiez R" first="Reinhard" last="Schwartz-Albiez">Reinhard Schwartz-Albiez</name><affiliation><mods:affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</mods:affiliation></affiliation></author><author><name sortKey="Lottspeich, Friedrich" sort="Lottspeich, Friedrich" uniqKey="Lottspeich F" first="Friedrich" last="Lottspeich">Friedrich Lottspeich</name><affiliation><mods:affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</mods:affiliation></affiliation></author><author><name sortKey="Meulen, Volker Ter" sort="Meulen, Volker Ter" uniqKey="Meulen V" first="Volker Ter" last="Meulen">Volker Ter Meulen</name><affiliation><mods:affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">198</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="265">265</biblScope><biblScope unit="page" to="274">274</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Measles virus is a highly contagious virus causing acute and persistent diseases in man, the receptor of which is still not well characterized. We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from 125I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. Our data suggest that moesin is acting as a receptor for measles virus.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Lee M. Dunster</name><affiliations><json:string>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</json:string></affiliations></json:item><json:item><name>Jürgen Schneider-Schaulies</name><affiliations><json:string>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</json:string></affiliations></json:item><json:item><name>Sieglinde Lüffler</name><affiliations><json:string>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</json:string></affiliations></json:item><json:item><name>Wolfgang Lankes</name><affiliations><json:string>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</json:string></affiliations></json:item><json:item><name>Reinhard Schwartz-Albiez</name><affiliations><json:string>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</json:string></affiliations></json:item><json:item><name>Friedrich Lottspeich</name><affiliations><json:string>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</json:string></affiliations></json:item><json:item><name>Volker Ter Meulen</name><affiliations><json:string>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-KDXDR28D-H</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Measles virus is a highly contagious virus causing acute and persistent diseases in man, the receptor of which is still not well characterized. We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from 125I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. 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Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Jürgen</forename><surname>Schneider-Schaulies</surname></persName><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Sieglinde</forename><surname>Lüffler</surname></persName><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Wolfgang</forename><surname>Lankes</surname></persName><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Reinhard</forename><surname>Schwartz-Albiez</surname></persName><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Friedrich</forename><surname>Lottspeich</surname></persName><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation></author><author xml:id="author-0006"><persName><forename type="first">Volker Ter</forename><surname>Meulen</surname></persName><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation></author><idno type="istex">284C220BE1609FE3A5AB6D2A1781EC88364DADFF</idno><idno type="ark">ark:/67375/6H6-KDXDR28D-H</idno><idno type="DOI">10.1006/viro.1994.1029</idno><idno type="PII">S0042-6822(84)71029-4</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0173-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994"></date><biblScope unit="volume">198</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="265">265</biblScope><biblScope unit="page" to="274">274</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1994</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Measles virus is a highly contagious virus causing acute and persistent diseases in man, the receptor of which is still not well characterized. We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from 125I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. Our data suggest that moesin is acting as a receptor for measles virus.</p></abstract></profileDesc><revisionDesc><change when="1994">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-KDXDR28D-H/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YVIRO</jid><aid>71029</aid><ce:pii>S0042-6822(84)71029-4</ce:pii><ce:doi>10.1006/viro.1994.1029</ce:doi><ce:copyright type="full-transfer" year="1994">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Moesin: A Cell Membrane Protein Linked with Susceptibility to Measles Virus Infection</ce:title><ce:author-group><ce:author><ce:given-name>Lee M.</ce:given-name><ce:surname>Dunster</ce:surname></ce:author><ce:author><ce:given-name>Jürgen</ce:given-name><ce:surname>Schneider-Schaulies</ce:surname></ce:author><ce:author><ce:given-name>Sieglinde</ce:given-name><ce:surname>Lüffler</ce:surname></ce:author><ce:author><ce:given-name>Wolfgang</ce:given-name><ce:surname>Lankes</ce:surname></ce:author><ce:author><ce:given-name>Reinhard</ce:given-name><ce:surname>Schwartz-Albiez</ce:surname></ce:author><ce:author><ce:given-name>Friedrich</ce:given-name><ce:surname>Lottspeich</ce:surname></ce:author><ce:author><ce:given-name>Volker Ter</ce:given-name><ce:surname>Meulen</ce:surname></ce:author><ce:affiliation><ce:textfn>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</ce:textfn></ce:affiliation></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Measles virus is a highly contagious virus causing acute and persistent diseases in man, the receptor of which is still not well characterized. We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from <ce:sup>125</ce:sup>I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. Our data suggest that moesin is acting as a receptor for measles virus.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Moesin: A Cell Membrane Protein Linked with Susceptibility to Measles Virus Infection</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Moesin: A Cell Membrane Protein Linked with Susceptibility to Measles Virus Infection</title></titleInfo><name type="personal"><namePart type="given">Lee M.</namePart><namePart type="family">Dunster</namePart><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jürgen</namePart><namePart type="family">Schneider-Schaulies</namePart><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sieglinde</namePart><namePart type="family">Lüffler</namePart><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wolfgang</namePart><namePart type="family">Lankes</namePart><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Reinhard</namePart><namePart type="family">Schwartz-Albiez</namePart><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Friedrich</namePart><namePart type="family">Lottspeich</namePart><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Volker Ter</namePart><namePart type="family">Meulen</namePart><affiliation>Institut für Virologie und Immunbiologie, Versbacher Strasse 7, D-97078 Würzburg, Germany; Medizinische Klinik I, Klinikum der RWTH Aachen, Pauwel Strasse 30, D-52074 Aachen, Germany; Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany; and Max Planck Institut für Biochemie, Am Klopferspitz, D- 82152 Martinsried, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Measles virus is a highly contagious virus causing acute and persistent diseases in man, the receptor of which is still not well characterized. We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from 125I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. Our data suggest that moesin is acting as a receptor for measles virus.</abstract><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0173-0</identifier><part><date>1994</date><detail type="volume"><number>198</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>413</end></extent><extent unit="pages"><start>265</start><end>274</end></extent></part></relatedItem><identifier type="istex">284C220BE1609FE3A5AB6D2A1781EC88364DADFF</identifier><identifier type="ark">ark:/67375/6H6-KDXDR28D-H</identifier><identifier type="DOI">10.1006/viro.1994.1029</identifier><identifier type="PII">S0042-6822(84)71029-4</identifier><accessCondition type="use and reproduction" contentType="copyright">©1994 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1994</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-KDXDR28D-H/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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