Response of rat tracheal epithelium to ozone and oxygen exposure in vitro
Identifieur interne : 000680 ( Istex/Corpus ); précédent : 000679; suivant : 000681Response of rat tracheal epithelium to ozone and oxygen exposure in vitro
Auteurs : Kristen J. Nikula ; Dennis W. WilsonSource :
- Fundamental and Applied Toxicology [ 0272-0590 ] ; 1990.
English descriptors
- Teeft :
- Amer, Basal, Basal cells, Carbon dioxide, Cell damage, Cell population, Cilium, Columnar, Culture medium, Dioxide, Epithelial, Epithelial injury, Epithelium, Intermediate cells, Metaplasia, Metaplastic, Metaplastic cells, Morphologic, Nikula, Nonciliated, Organ culture, Oxidant, Oxygen exposure, Ozone, Ozone exposure, Preciliated cells, Predominant cell types, Pseudostratified, Respiratory epithelium, Secretory, Secretory cells, Secretory granules, Serous, Serous cells, Sigler, Sloughed, Sloughed cells, Squamous metaplasia, Statistical significance level, Trachea, Tracheal, Tracheal epithelium, Tracheal organ cultures, Ultrastructural, Ultrastructural study, Vivo.
Abstract
Abstract: Although ozone-induced epithelial injury in vivo has been morphologically characterized, effects of gaseous oxidants on respiratory epithelium in organ culture, where tissue organization is maintained but systemic influences are eliminated, have not been thoroughly investigated. In this study, we exposed tracheal organ cultures from rats to 95% oxygen and 1 ppm ozone, alone and in combination, to determine (1) whether epithelial responses to ozone similar to those observed in vivo occur in airways separated from systemic physiologic, secretory, and inflammatory reactions; (2) whether concentrations of oxygen sufficient to potentially cause oxidant injury result in morphologic epithelial alterations similar to those that occur in ozone toxicity; and (3) if the combined oxidant insult of oxygen and ozone results in more severe damage to the tracheal epithelium than occurs with ozone in air. Tracheal organ cultures were exposed to filtered air and 5% carbon dioxide; filtered air, 5% carbon dioxide, and 1 ppm ozone; 95% oxygen and 5% carbon dioxide; or 95% oxygen, 5% carbon dioxide, and 1 ppm ozone for 96 hr. Light- and quantitative electron-microscopic evaluation showed that epithelia to 1 ppm ozone in air exhibited loss of ciliated cells and ciliated cell damage. The epithelia exposed to 95% oxygen and 5% carbon dioxide were pseudostratified, columnar, ciliated, and hyperplastic. Epithelia exposed to 95% oxygen plus 1 ppm ozone were stratified and nonciliated or very sparsely ciliated. The predominant cell types in epithelia exposed to oxygen plus ozone were serous cells and metaplastic cells, and focal aggregates of adherent necrotic cells were present. We conclude that there was a synergism between oxygen and ozone exposure leading to enhanced epithelial injury and metaplasia.
Url:
DOI: 10.1016/0272-0590(90)90169-K
Links to Exploration step
ISTEX:0D42C950F56AE170C6C68AC71EE2BC4FDE138768Le document en format XML
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In this study, we exposed tracheal organ cultures from rats to 95% oxygen and 1 ppm ozone, alone and in combination, to determine (1) whether epithelial responses to ozone similar to those observed in vivo occur in airways separated from systemic physiologic, secretory, and inflammatory reactions; (2) whether concentrations of oxygen sufficient to potentially cause oxidant injury result in morphologic epithelial alterations similar to those that occur in ozone toxicity; and (3) if the combined oxidant insult of oxygen and ozone results in more severe damage to the tracheal epithelium than occurs with ozone in air. Tracheal organ cultures were exposed to filtered air and 5% carbon dioxide; filtered air, 5% carbon dioxide, and 1 ppm ozone; 95% oxygen and 5% carbon dioxide; or 95% oxygen, 5% carbon dioxide, and 1 ppm ozone for 96 hr. Light- and quantitative electron-microscopic evaluation showed that epithelia to 1 ppm ozone in air exhibited loss of ciliated cells and ciliated cell damage. The epithelia exposed to 95% oxygen and 5% carbon dioxide were pseudostratified, columnar, ciliated, and hyperplastic. Epithelia exposed to 95% oxygen plus 1 ppm ozone were stratified and nonciliated or very sparsely ciliated. The predominant cell types in epithelia exposed to oxygen plus ozone were serous cells and metaplastic cells, and focal aggregates of adherent necrotic cells were present. We conclude that there was a synergism between oxygen and ozone exposure leading to enhanced epithelial injury and metaplasia.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>epithelium</json:string><json:string>tracheal</json:string><json:string>basal</json:string><json:string>metaplastic</json:string><json:string>serous</json:string><json:string>epithelial</json:string><json:string>oxidant</json:string><json:string>tracheal epithelium</json:string><json:string>metaplastic cells</json:string><json:string>carbon dioxide</json:string><json:string>nikula</json:string><json:string>serous cells</json:string><json:string>basal cells</json:string><json:string>columnar</json:string><json:string>secretory</json:string><json:string>ozone</json:string><json:string>intermediate cells</json:string><json:string>ultrastructural</json:string><json:string>oxygen exposure</json:string><json:string>amer</json:string><json:string>nonciliated</json:string><json:string>sigler</json:string><json:string>cilium</json:string><json:string>sloughed</json:string><json:string>metaplasia</json:string><json:string>pseudostratified</json:string><json:string>ozone exposure</json:string><json:string>tracheal organ cultures</json:string><json:string>respiratory epithelium</json:string><json:string>organ culture</json:string><json:string>dioxide</json:string><json:string>sloughed cells</json:string><json:string>secretory cells</json:string><json:string>preciliated cells</json:string><json:string>cell damage</json:string><json:string>cell population</json:string><json:string>morphologic</json:string><json:string>trachea</json:string><json:string>predominant cell types</json:string><json:string>statistical significance level</json:string><json:string>squamous metaplasia</json:string><json:string>epithelial injury</json:string><json:string>ultrastructural study</json:string><json:string>culture medium</json:string><json:string>secretory granules</json:string><json:string>vivo</json:string></teeft></keywords><author><json:item><name>Kristen J. Nikula</name><affiliations><json:string>Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, P.O. Box 5890, Albuquerque, New Mexico 87185 USA</json:string></affiliations></json:item><json:item><name>Dennis W. Wilson</name><affiliations><json:string>Department of Pathology, School of Veterinary Medicine, University of California, Davis, California 95616 USA</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-5044MGFM-0</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Although ozone-induced epithelial injury in vivo has been morphologically characterized, effects of gaseous oxidants on respiratory epithelium in organ culture, where tissue organization is maintained but systemic influences are eliminated, have not been thoroughly investigated. In this study, we exposed tracheal organ cultures from rats to 95% oxygen and 1 ppm ozone, alone and in combination, to determine (1) whether epithelial responses to ozone similar to those observed in vivo occur in airways separated from systemic physiologic, secretory, and inflammatory reactions; (2) whether concentrations of oxygen sufficient to potentially cause oxidant injury result in morphologic epithelial alterations similar to those that occur in ozone toxicity; and (3) if the combined oxidant insult of oxygen and ozone results in more severe damage to the tracheal epithelium than occurs with ozone in air. Tracheal organ cultures were exposed to filtered air and 5% carbon dioxide; filtered air, 5% carbon dioxide, and 1 ppm ozone; 95% oxygen and 5% carbon dioxide; or 95% oxygen, 5% carbon dioxide, and 1 ppm ozone for 96 hr. Light- and quantitative electron-microscopic evaluation showed that epithelia to 1 ppm ozone in air exhibited loss of ciliated cells and ciliated cell damage. The epithelia exposed to 95% oxygen and 5% carbon dioxide were pseudostratified, columnar, ciliated, and hyperplastic. Epithelia exposed to 95% oxygen plus 1 ppm ozone were stratified and nonciliated or very sparsely ciliated. The predominant cell types in epithelia exposed to oxygen plus ozone were serous cells and metaplastic cells, and focal aggregates of adherent necrotic cells were present. 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(1973)</json:string><json:string>Clark and Marchok, 1979</json:string><json:string>Wilson et al., 1984</json:string><json:string>Heino and Laitinen, 1982</json:string><json:string>Pavelka, 1976</json:string><json:string>Palekar et al.. 1968</json:string><json:string>Mustafa and Tierney, 1978</json:string><json:string>Nikula et al., 1988</json:string><json:string>Philpott et al.. 1977</json:string><json:string>Jeffery and Reid, 1975</json:string><json:string>Sigler et al.</json:string><json:string>Pearsall et al., 1984</json:string><json:string>Chang et al., 1985</json:string><json:string>Sigler et al., 1987, 1988</json:string><json:string>Mossman and Craighead, 1975</json:string><json:string>Jeffrey and Reid, 1975</json:string><json:string>Marchok et al., 1975</json:string><json:string>Breeze and Wheeldon, 1977</json:string><json:string>Sigler et al., 1988</json:string><json:string>Inayama et al. (1988)</json:string><json:string>Environmental Protection Agency, 1980</json:string><json:string>Chopra and Cooney, 1985</json:string><json:string>Nikula et al.. 1988</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-5044MGFM-0</json:string></ark><categories><wos></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - toxicology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Pharmacology, Toxicology and Pharmaceutics</json:string><json:string>3 - Toxicology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1990</publicationDate><copyrightDate>1990</copyrightDate><doi><json:string>10.1016/0272-0590(90)90169-K</json:string></doi><id>0D42C950F56AE170C6C68AC71EE2BC4FDE138768</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5044MGFM-0/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5044MGFM-0/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-5044MGFM-0/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Response of rat tracheal epithelium to ozone and oxygen exposure in vitro</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1990</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Regular article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Response of rat tracheal epithelium to ozone and oxygen exposure in vitro</title><author xml:id="author-0000"><persName><forename type="first">Kristen J.</forename><surname>Nikula</surname></persName><affiliation>Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, P.O. Box 5890, Albuquerque, New Mexico 87185 USA</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Dennis W.</forename><surname>Wilson</surname></persName><affiliation>Department of Pathology, School of Veterinary Medicine, University of California, Davis, California 95616 USA</affiliation></author><idno type="istex">0D42C950F56AE170C6C68AC71EE2BC4FDE138768</idno><idno type="ark">ark:/67375/6H6-5044MGFM-0</idno><idno type="DOI">10.1016/0272-0590(90)90169-K</idno><idno type="PII">0272-0590(90)90169-K</idno></analytic><monogr><title level="j">Fundamental and Applied Toxicology</title><title level="j" type="abbrev">YFAAT</title><idno type="pISSN">0272-0590</idno><idno type="PII">S0272-0590(00)X0116-9</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990"></date><biblScope unit="volume">15</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="121">121</biblScope><biblScope unit="page" to="131">131</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1990</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Although ozone-induced epithelial injury in vivo has been morphologically characterized, effects of gaseous oxidants on respiratory epithelium in organ culture, where tissue organization is maintained but systemic influences are eliminated, have not been thoroughly investigated. In this study, we exposed tracheal organ cultures from rats to 95% oxygen and 1 ppm ozone, alone and in combination, to determine (1) whether epithelial responses to ozone similar to those observed in vivo occur in airways separated from systemic physiologic, secretory, and inflammatory reactions; (2) whether concentrations of oxygen sufficient to potentially cause oxidant injury result in morphologic epithelial alterations similar to those that occur in ozone toxicity; and (3) if the combined oxidant insult of oxygen and ozone results in more severe damage to the tracheal epithelium than occurs with ozone in air. Tracheal organ cultures were exposed to filtered air and 5% carbon dioxide; filtered air, 5% carbon dioxide, and 1 ppm ozone; 95% oxygen and 5% carbon dioxide; or 95% oxygen, 5% carbon dioxide, and 1 ppm ozone for 96 hr. Light- and quantitative electron-microscopic evaluation showed that epithelia to 1 ppm ozone in air exhibited loss of ciliated cells and ciliated cell damage. The epithelia exposed to 95% oxygen and 5% carbon dioxide were pseudostratified, columnar, ciliated, and hyperplastic. Epithelia exposed to 95% oxygen plus 1 ppm ozone were stratified and nonciliated or very sparsely ciliated. The predominant cell types in epithelia exposed to oxygen plus ozone were serous cells and metaplastic cells, and focal aggregates of adherent necrotic cells were present. We conclude that there was a synergism between oxygen and ozone exposure leading to enhanced epithelial injury and metaplasia.</p></abstract></profileDesc><revisionDesc><change when="1990">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5044MGFM-0/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YFAAT</jid><aid>9090169K</aid><ce:pii>0272-0590(90)90169-K</ce:pii><ce:doi>10.1016/0272-0590(90)90169-K</ce:doi><ce:copyright type="unknown" year="1990"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular article</ce:textfn></ce:dochead><ce:title>Response of rat tracheal epithelium to ozone and oxygen exposure <ce:italic>in vitro</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>Kristen J.</ce:given-name><ce:surname>Nikula</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Dennis W.</ce:given-name><ce:surname>Wilson</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, P.O. Box 5890, Albuquerque, New Mexico 87185 USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Pathology, School of Veterinary Medicine, University of California, Davis, California 95616 USA</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="22" month="9" year="1989"></ce:date-received><ce:date-accepted day="22" month="1" year="1990"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Although ozone-induced epithelial injury <ce:italic>in vivo</ce:italic> has been morphologically characterized, effects of gaseous oxidants on respiratory epithelium in organ culture, where tissue organization is maintained but systemic influences are eliminated, have not been thoroughly investigated. In this study, we exposed tracheal organ cultures from rats to 95% oxygen and 1 ppm ozone, alone and in combination, to determine (1) whether epithelial responses to ozone similar to those observed <ce:italic>in vivo</ce:italic> occur in airways separated from systemic physiologic, secretory, and inflammatory reactions; (2) whether concentrations of oxygen sufficient to potentially cause oxidant injury result in morphologic epithelial alterations similar to those that occur in ozone toxicity; and (3) if the combined oxidant insult of oxygen and ozone results in more severe damage to the tracheal epithelium than occurs with ozone in air. Tracheal organ cultures were exposed to filtered air and 5% carbon dioxide; filtered air, 5% carbon dioxide, and 1 ppm ozone; 95% oxygen and 5% carbon dioxide; or 95% oxygen, 5% carbon dioxide, and 1 ppm ozone for 96 hr. Light- and quantitative electron-microscopic evaluation showed that epithelia to 1 ppm ozone in air exhibited loss of ciliated cells and ciliated cell damage. The epithelia exposed to 95% oxygen and 5% carbon dioxide were pseudostratified, columnar, ciliated, and hyperplastic. Epithelia exposed to 95% oxygen plus 1 ppm ozone were stratified and nonciliated or very sparsely ciliated. The predominant cell types in epithelia exposed to oxygen plus ozone were serous cells and metaplastic cells, and focal aggregates of adherent necrotic cells were present. We conclude that there was a synergism between oxygen and ozone exposure leading to enhanced epithelial injury and metaplasia.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Response of rat tracheal epithelium to ozone and oxygen exposure in vitro</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Response of rat tracheal epithelium to ozone and oxygen exposure</title></titleInfo><name type="personal"><namePart type="given">Kristen J.</namePart><namePart type="family">Nikula</namePart><affiliation>Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, P.O. Box 5890, Albuquerque, New Mexico 87185 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dennis W.</namePart><namePart type="family">Wilson</namePart><affiliation>Department of Pathology, School of Veterinary Medicine, University of California, Davis, California 95616 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued><copyrightDate encoding="w3cdtf">1990</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Although ozone-induced epithelial injury in vivo has been morphologically characterized, effects of gaseous oxidants on respiratory epithelium in organ culture, where tissue organization is maintained but systemic influences are eliminated, have not been thoroughly investigated. In this study, we exposed tracheal organ cultures from rats to 95% oxygen and 1 ppm ozone, alone and in combination, to determine (1) whether epithelial responses to ozone similar to those observed in vivo occur in airways separated from systemic physiologic, secretory, and inflammatory reactions; (2) whether concentrations of oxygen sufficient to potentially cause oxidant injury result in morphologic epithelial alterations similar to those that occur in ozone toxicity; and (3) if the combined oxidant insult of oxygen and ozone results in more severe damage to the tracheal epithelium than occurs with ozone in air. Tracheal organ cultures were exposed to filtered air and 5% carbon dioxide; filtered air, 5% carbon dioxide, and 1 ppm ozone; 95% oxygen and 5% carbon dioxide; or 95% oxygen, 5% carbon dioxide, and 1 ppm ozone for 96 hr. Light- and quantitative electron-microscopic evaluation showed that epithelia to 1 ppm ozone in air exhibited loss of ciliated cells and ciliated cell damage. The epithelia exposed to 95% oxygen and 5% carbon dioxide were pseudostratified, columnar, ciliated, and hyperplastic. Epithelia exposed to 95% oxygen plus 1 ppm ozone were stratified and nonciliated or very sparsely ciliated. The predominant cell types in epithelia exposed to oxygen plus ozone were serous cells and metaplastic cells, and focal aggregates of adherent necrotic cells were present. We conclude that there was a synergism between oxygen and ozone exposure leading to enhanced epithelial injury and metaplasia.</abstract><note type="content">Section title: Regular article</note><relatedItem type="host"><titleInfo><title>Fundamental and Applied Toxicology</title></titleInfo><titleInfo type="abbreviated"><title>YFAAT</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued></originInfo><identifier type="ISSN">0272-0590</identifier><identifier type="PII">S0272-0590(00)X0116-9</identifier><part><date>1990</date><detail type="volume"><number>15</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>212</end></extent><extent unit="pages"><start>121</start><end>131</end></extent></part></relatedItem><identifier type="istex">0D42C950F56AE170C6C68AC71EE2BC4FDE138768</identifier><identifier type="ark">ark:/67375/6H6-5044MGFM-0</identifier><identifier type="DOI">10.1016/0272-0590(90)90169-K</identifier><identifier type="PII">0272-0590(90)90169-K</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5044MGFM-0/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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