Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)
Identifieur interne : 000677 ( Istex/Corpus ); précédent : 000676; suivant : 000678Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)
Auteurs : B. Charley ; L. LavenantSource :
- Research in Immunology [ 0923-2494 ] ; 1990.
English descriptors
- Teeft :
- Alpha interferon, Antiviral activity, Ascitic fluids, Cell monolayers, Cell surface glycoproteins, Cellules, Cellules infect6es, Coronavirus, Dendritic cells, Dengue virus, Direct effect, Final dilution, Gastroenteritis, Glycoprotein, Herpes, Herpes simplex virus, Human lymphocytes, Ifn0t, Ifn0t induction process, Ifn0t production, Ifna, Ifna production, Igg2a, Induction, Influenza virus, Interferon, Interferon production, Kidney cells, Laude, Lebon, Lesser extent, Leukocyte, Lymphocyte, Major histocompatibility, Membrane interactions, Membrane structures, Monoclonal, Monoclonal antibodies, Monoclonal antibodies reactive, Monolayers, Msa3, Msa3 hybridoma culture, Mycoplasma membranes, Other antisla class, Overnight incubation, Pbmc, Pbmc responsiveness, Porcine, Porcine cells, Pretreatment, Producer cells, Rabbit serum, Sendai virus, Surface antigens, Tgev, Transmissible gastroenteritis coronavirus, Transmissible gastroenteritis virus, Viable cells, Viral glycoprotein, Virus particles.
Abstract
Abstract: Porcine blood mononuclear cells (PBMC) were shown to produce interferon-α (IFNα) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. Monoclonal antibodies (mAb) with specificities for leukocyte subsets and major histocompatibility complex (MHC) antigens were used to characterize IFNα producer cells. The production of IFNα was found to be a function of non-phagocytic, non-adherent, non-T, non-B, CD4+ (and to a lesser extent CD8+) MHC-class-II-positive cells. Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction.
Url:
DOI: 10.1016/0923-2494(90)90133-J
Links to Exploration step
ISTEX:44EE75741B0741123E24817D35B40C8FF2F998E0Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)</title><author><name sortKey="Charley, B" sort="Charley, B" uniqKey="Charley B" first="B." last="Charley">B. Charley</name><affiliation><mods:affiliation>Laboratoire de Virologie et d'Immunologie moléculaires, INRA, 78350 Jouy-en-Josas France</mods:affiliation></affiliation></author><author><name sortKey="Lavenant, L" sort="Lavenant, L" uniqKey="Lavenant L" first="L." last="Lavenant">L. Lavenant</name><affiliation><mods:affiliation>Laboratoire de Virologie et d'Immunologie moléculaires, INRA, 78350 Jouy-en-Josas France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:44EE75741B0741123E24817D35B40C8FF2F998E0</idno><date when="1990" year="1990">1990</date><idno type="doi">10.1016/0923-2494(90)90133-J</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-DM18KW73-L/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000677</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000677</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)</title><author><name sortKey="Charley, B" sort="Charley, B" uniqKey="Charley B" first="B." last="Charley">B. Charley</name><affiliation><mods:affiliation>Laboratoire de Virologie et d'Immunologie moléculaires, INRA, 78350 Jouy-en-Josas France</mods:affiliation></affiliation></author><author><name sortKey="Lavenant, L" sort="Lavenant, L" uniqKey="Lavenant L" first="L." last="Lavenant">L. Lavenant</name><affiliation><mods:affiliation>Laboratoire de Virologie et d'Immunologie moléculaires, INRA, 78350 Jouy-en-Josas France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Research in Immunology</title><title level="j" type="abbrev">X83</title><idno type="ISSN">0923-2494</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990">1990</date><biblScope unit="volume">141</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="141">141</biblScope><biblScope unit="page" to="151">151</biblScope></imprint><idno type="ISSN">0923-2494</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0923-2494</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Alpha interferon</term><term>Antiviral activity</term><term>Ascitic fluids</term><term>Cell monolayers</term><term>Cell surface glycoproteins</term><term>Cellules</term><term>Cellules infect6es</term><term>Coronavirus</term><term>Dendritic cells</term><term>Dengue virus</term><term>Direct effect</term><term>Final dilution</term><term>Gastroenteritis</term><term>Glycoprotein</term><term>Herpes</term><term>Herpes simplex virus</term><term>Human lymphocytes</term><term>Ifn0t</term><term>Ifn0t induction process</term><term>Ifn0t production</term><term>Ifna</term><term>Ifna production</term><term>Igg2a</term><term>Induction</term><term>Influenza virus</term><term>Interferon</term><term>Interferon production</term><term>Kidney cells</term><term>Laude</term><term>Lebon</term><term>Lesser extent</term><term>Leukocyte</term><term>Lymphocyte</term><term>Major histocompatibility</term><term>Membrane interactions</term><term>Membrane structures</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Monoclonal antibodies reactive</term><term>Monolayers</term><term>Msa3</term><term>Msa3 hybridoma culture</term><term>Mycoplasma membranes</term><term>Other antisla class</term><term>Overnight incubation</term><term>Pbmc</term><term>Pbmc responsiveness</term><term>Porcine</term><term>Porcine cells</term><term>Pretreatment</term><term>Producer cells</term><term>Rabbit serum</term><term>Sendai virus</term><term>Surface antigens</term><term>Tgev</term><term>Transmissible gastroenteritis coronavirus</term><term>Transmissible gastroenteritis virus</term><term>Viable cells</term><term>Viral glycoprotein</term><term>Virus particles</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Porcine blood mononuclear cells (PBMC) were shown to produce interferon-α (IFNα) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. Monoclonal antibodies (mAb) with specificities for leukocyte subsets and major histocompatibility complex (MHC) antigens were used to characterize IFNα producer cells. The production of IFNα was found to be a function of non-phagocytic, non-adherent, non-T, non-B, CD4+ (and to a lesser extent CD8+) MHC-class-II-positive cells. Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>pbmc</json:string><json:string>lymphocyte</json:string><json:string>interferon</json:string><json:string>porcine</json:string><json:string>ifna</json:string><json:string>monolayers</json:string><json:string>leukocyte</json:string><json:string>cellules</json:string><json:string>monoclonal</json:string><json:string>coronavirus</json:string><json:string>pretreatment</json:string><json:string>ifn0t</json:string><json:string>tgev</json:string><json:string>monoclonal antibodies</json:string><json:string>herpes</json:string><json:string>msa3</json:string><json:string>glycoprotein</json:string><json:string>gastroenteritis</json:string><json:string>lebon</json:string><json:string>igg2a</json:string><json:string>cell monolayers</json:string><json:string>transmissible gastroenteritis virus</json:string><json:string>laude</json:string><json:string>membrane interactions</json:string><json:string>porcine cells</json:string><json:string>dendritic cells</json:string><json:string>lesser extent</json:string><json:string>alpha interferon</json:string><json:string>human lymphocytes</json:string><json:string>induction</json:string><json:string>surface antigens</json:string><json:string>ascitic fluids</json:string><json:string>rabbit serum</json:string><json:string>final dilution</json:string><json:string>overnight incubation</json:string><json:string>kidney cells</json:string><json:string>ifn0t induction process</json:string><json:string>producer cells</json:string><json:string>ifn0t production</json:string><json:string>viable cells</json:string><json:string>pbmc responsiveness</json:string><json:string>ifna production</json:string><json:string>msa3 hybridoma culture</json:string><json:string>direct effect</json:string><json:string>sendai virus</json:string><json:string>viral glycoprotein</json:string><json:string>influenza virus</json:string><json:string>mycoplasma membranes</json:string><json:string>dengue virus</json:string><json:string>cell surface glycoproteins</json:string><json:string>membrane structures</json:string><json:string>major histocompatibility</json:string><json:string>cellules infect6es</json:string><json:string>transmissible gastroenteritis coronavirus</json:string><json:string>virus particles</json:string><json:string>monoclonal antibodies reactive</json:string><json:string>antiviral activity</json:string><json:string>interferon production</json:string><json:string>herpes simplex virus</json:string><json:string>other antisla class</json:string></teeft></keywords><author><json:item><name>B. 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Lavenant</name><affiliations><json:string>Laboratoire de Virologie et d'Immunologie moléculaires, INRA, 78350 Jouy-en-Josas France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Interferon-α, Leukocyte, MHC, Coronavirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Transmissible gastroenteritis virus, mAb, PBMC</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Interféron alpha, Lymphocyte, CMH, Coronavirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Anticorps monoclonaux, Virus de la Gastroentérite transmissible du porc</value></json:item></subject><arkIstex>ark:/67375/6H6-DM18KW73-L</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Porcine blood mononuclear cells (PBMC) were shown to produce interferon-α (IFNα) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. Monoclonal antibodies (mAb) with specificities for leukocyte subsets and major histocompatibility complex (MHC) antigens were used to characterize IFNα producer cells. The production of IFNα was found to be a function of non-phagocytic, non-adherent, non-T, non-B, CD4+ (and to a lesser extent CD8+) MHC-class-II-positive cells. Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction.</abstract><qualityIndicators><score>7.045</score><pdfWordCount>3881</pdfWordCount><pdfCharCount>22726</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>11</pdfPageCount><pdfPageSize>548 x 771 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>97</abstractWordCount><abstractCharCount>728</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)</title><pmid><json:string>2167506</json:string></pmid><pii><json:string>0923-2494(90)90133-J</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Soc. exp. Biol. 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(1987)</json:string><json:string>La Bonnardiere and Laude, 1981</json:string><json:string>Kirchner et aL, 1979</json:string><json:string>Fitzgerald-Bocarsly et aL, 1988</json:string><json:string>Dianzani and Capobianchi, 1987</json:string><json:string>La Bonnardi6re and Laude, 1981</json:string><json:string>Salmon et al., 1989</json:string><json:string>Lebon et aL, 1982</json:string><json:string>Pescovitz et aL, 1984</json:string><json:string>Reiss et al., 1984</json:string><json:string>Fitzgerald-Bocarsky et al., 1988</json:string><json:string>Lebon et al., 1982</json:string><json:string>Charley and Laurie, 1988</json:string><json:string>Abb et aL, 1983</json:string><json:string>Kurane et al., 1986</json:string><json:string>Capobianchi et al., 1987</json:string><json:string>Perussia et al., 1985</json:string><json:string>Peter et aL, 1980</json:string><json:string>Capobianchi et al.., 1987</json:string><json:string>Lebon, 1985</json:string><json:string>Kirchner et al., 1979</json:string><json:string>Perussia et aL, 1985</json:string><json:string>Sandberg et al., 1989b</json:string><json:string>Abb et al., 1983</json:string><json:string>Oh et al., 1987</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-DM18KW73-L</json:string></ark><categories><wos></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - immunology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Immunology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1990</publicationDate><copyrightDate>1990</copyrightDate><doi><json:string>10.1016/0923-2494(90)90133-J</json:string></doi><id>44EE75741B0741123E24817D35B40C8FF2F998E0</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-DM18KW73-L/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-DM18KW73-L/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-DM18KW73-L/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1990</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)</title><author xml:id="author-0000"><persName><forename type="first">B.</forename><surname>Charley</surname></persName><affiliation>Laboratoire de Virologie et d'Immunologie moléculaires, INRA, 78350 Jouy-en-Josas France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">L.</forename><surname>Lavenant</surname></persName><affiliation>Laboratoire de Virologie et d'Immunologie moléculaires, INRA, 78350 Jouy-en-Josas France</affiliation></author><idno type="istex">44EE75741B0741123E24817D35B40C8FF2F998E0</idno><idno type="ark">ark:/67375/6H6-DM18KW73-L</idno><idno type="DOI">10.1016/0923-2494(90)90133-J</idno><idno type="PII">0923-2494(90)90133-J</idno></analytic><monogr><title level="j">Research in Immunology</title><title level="j" type="abbrev">X83</title><idno type="pISSN">0923-2494</idno><idno type="PII">S0923-2494(00)X0017-X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990"></date><biblScope unit="volume">141</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="141">141</biblScope><biblScope unit="page" to="151">151</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1990</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Porcine blood mononuclear cells (PBMC) were shown to produce interferon-α (IFNα) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. Monoclonal antibodies (mAb) with specificities for leukocyte subsets and major histocompatibility complex (MHC) antigens were used to characterize IFNα producer cells. The production of IFNα was found to be a function of non-phagocytic, non-adherent, non-T, non-B, CD4+ (and to a lesser extent CD8+) MHC-class-II-positive cells. Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Interferon-α, Leukocyte, MHC, Coronavirus</term></item><item><term>Transmissible gastroenteritis virus, mAb, PBMC</term></item></list></keywords></textClass><textClass xml:lang="fr"><keywords scheme="keyword"><list><head>Mots-clé</head><item><term>Interféron alpha, Lymphocyte, CMH, Coronavirus</term></item><item><term>Anticorps monoclonaux, Virus de la Gastroentérite transmissible du porc</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1990">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-DM18KW73-L/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>X83</jid><aid>9090133J</aid><ce:pii>0923-2494(90)90133-J</ce:pii><ce:doi>10.1016/0923-2494(90)90133-J</ce:doi><ce:copyright type="unknown" year="1990"></ce:copyright></item-info><head><ce:title>Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)</ce:title><ce:author-group><ce:author><ce:given-name>B.</ce:given-name><ce:surname>Charley</ce:surname></ce:author><ce:author><ce:given-name>L.</ce:given-name><ce:surname>Lavenant</ce:surname></ce:author><ce:affiliation><ce:textfn>Laboratoire de Virologie et d'Immunologie moléculaires, INRA, 78350 Jouy-en-Josas France</ce:textfn></ce:affiliation></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Porcine blood mononuclear cells (PBMC) were shown to produce interferon-α (IFNα) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. Monoclonal antibodies (mAb) with specificities for leukocyte subsets and major histocompatibility complex (MHC) antigens were used to characterize IFNα producer cells. The production of IFNα was found to be a function of non-phagocytic, non-adherent, non-T, non-B, CD4<ce:sup>+</ce:sup> (and to a lesser extent CD8<ce:sup>+</ce:sup>) MHC-class-II-positive cells. Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Interferon-α, Leukocyte, MHC, Coronavirus</ce:text></ce:keyword><ce:keyword><ce:text>Transmissible gastroenteritis virus, mAb, PBMC</ce:text></ce:keyword></ce:keywords><ce:keywords xml:lang="fr"><ce:section-title>Mots-clé</ce:section-title><ce:keyword><ce:text>Interféron alpha, Lymphocyte, CMH, Coronavirus</ce:text></ce:keyword><ce:keyword><ce:text>Anticorps monoclonaux, Virus de la Gastroentérite transmissible du porc</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)</title></titleInfo><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Charley</namePart><affiliation>Laboratoire de Virologie et d'Immunologie moléculaires, INRA, 78350 Jouy-en-Josas France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Lavenant</namePart><affiliation>Laboratoire de Virologie et d'Immunologie moléculaires, INRA, 78350 Jouy-en-Josas France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued><copyrightDate encoding="w3cdtf">1990</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Porcine blood mononuclear cells (PBMC) were shown to produce interferon-α (IFNα) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. Monoclonal antibodies (mAb) with specificities for leukocyte subsets and major histocompatibility complex (MHC) antigens were used to characterize IFNα producer cells. The production of IFNα was found to be a function of non-phagocytic, non-adherent, non-T, non-B, CD4+ (and to a lesser extent CD8+) MHC-class-II-positive cells. Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction.</abstract><subject><genre>Keywords</genre><topic>Interferon-α, Leukocyte, MHC, Coronavirus</topic><topic>Transmissible gastroenteritis virus, mAb, PBMC</topic></subject><subject lang="fr"><genre>Mots-clé</genre><topic>Interféron alpha, Lymphocyte, CMH, Coronavirus</topic><topic>Anticorps monoclonaux, Virus de la Gastroentérite transmissible du porc</topic></subject><relatedItem type="host"><titleInfo><title>Research in Immunology</title></titleInfo><titleInfo type="abbreviated"><title>X83</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued></originInfo><identifier type="ISSN">0923-2494</identifier><identifier type="PII">S0923-2494(00)X0017-X</identifier><part><date>1990</date><detail type="volume"><number>141</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>117</start><end>230</end></extent><extent unit="pages"><start>141</start><end>151</end></extent></part></relatedItem><identifier type="istex">44EE75741B0741123E24817D35B40C8FF2F998E0</identifier><identifier type="ark">ark:/67375/6H6-DM18KW73-L</identifier><identifier type="DOI">10.1016/0923-2494(90)90133-J</identifier><identifier type="PII">0923-2494(90)90133-J</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-DM18KW73-L/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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