Expression of the Aminopeptidase N (CD13) Gene in the Human T Cell Lines HuT78 and H9
Identifieur interne : 000670 ( Istex/Corpus ); précédent : 000669; suivant : 000671Expression of the Aminopeptidase N (CD13) Gene in the Human T Cell Lines HuT78 and H9
Auteurs : U. Lendeckel ; Th. Wex ; Th. K Hne ; K. Frank ; D. Reinhold ; S. AnsorgeSource :
- Cellular Immunology [ 0008-8749 ] ; 1994.
Abstract
Abstract: The membrane-bound metalloprotease aminopeptidase N (APN, CD13, EC 3.4.11.2) is a well-established marker of normal and malignant cells of the myelomonocytic lineages. It is also expressed by leukemic blasts of a small group of patients suffering from acute or chronic lymphoid leukemia. CD13-specific monoclonal antibodies do not bind to the surface of normal B lymphocytes and APN-mRNA was not detectable by Northern analysis in normal lymphocytes or in T cell lines. A recent paper, however, describes the expression of CD13 on concanavalin A-stimulated T cells. Here, by means of enzymatic amplification of cDNA, cloning and sequencing of amplified cDNA fragments, in situ hybridization, and transcription start site mapping, evidence is provided that the human T cell lines HuT78 and H9 do contain APN-mRNA. Ala-pNA-hydrolyzing activity was detected in viable cells and cell homogenates as well as in size-fractionated protein fractions. Alanine-β-naphthylamide (Ala-βNA)-hydrolyzing activity was readily detectable after isoelectric focusing and blotting and was shown to be localized exclusively intracellularly in both cell lines. Usage of aminopeptidase-specific effectors revealed this activity to be distinct from CD13.
Url:
DOI: 10.1006/cimm.1994.1019
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Ansorge</name><affiliation><mods:affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cellular Immunology</title><title level="j" type="abbrev">YCIMM</title><idno type="ISSN">0008-8749</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">153</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="214">214</biblScope><biblScope unit="page" to="226">226</biblScope></imprint><idno type="ISSN">0008-8749</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0008-8749</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The membrane-bound metalloprotease aminopeptidase N (APN, CD13, EC 3.4.11.2) is a well-established marker of normal and malignant cells of the myelomonocytic lineages. It is also expressed by leukemic blasts of a small group of patients suffering from acute or chronic lymphoid leukemia. CD13-specific monoclonal antibodies do not bind to the surface of normal B lymphocytes and APN-mRNA was not detectable by Northern analysis in normal lymphocytes or in T cell lines. A recent paper, however, describes the expression of CD13 on concanavalin A-stimulated T cells. Here, by means of enzymatic amplification of cDNA, cloning and sequencing of amplified cDNA fragments, in situ hybridization, and transcription start site mapping, evidence is provided that the human T cell lines HuT78 and H9 do contain APN-mRNA. Ala-pNA-hydrolyzing activity was detected in viable cells and cell homogenates as well as in size-fractionated protein fractions. Alanine-β-naphthylamide (Ala-βNA)-hydrolyzing activity was readily detectable after isoelectric focusing and blotting and was shown to be localized exclusively intracellularly in both cell lines. 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Aminopeptidase N (CD13) Gene in the Human T Cell Lines HuT78 and H9</title><author xml:id="author-0000"><persName><forename type="first">U.</forename><surname>Lendeckel</surname></persName><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Th.</forename><surname>Wex</surname></persName><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Th.</forename><surname>Kähne</surname></persName><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation></author><author xml:id="author-0003"><persName><forename type="first">K.</forename><surname>Frank</surname></persName><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation></author><author xml:id="author-0004"><persName><forename type="first">D.</forename><surname>Reinhold</surname></persName><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation></author><author xml:id="author-0005"><persName><forename type="first">S.</forename><surname>Ansorge</surname></persName><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation></author><idno type="istex">EEDE35298F7A4A95DA30E2558AD5CFFDA2971763</idno><idno type="ark">ark:/67375/6H6-Z3MR9B50-Z</idno><idno type="DOI">10.1006/cimm.1994.1019</idno><idno type="PII">S0008-8749(84)71019-7</idno><idno type="article-id">71019</idno></analytic><monogr><title level="j">Cellular Immunology</title><title level="j" type="abbrev">YCIMM</title><idno type="pISSN">0008-8749</idno><idno type="PII">S0008-8749(00)X0118-9</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994"></date><biblScope unit="volume">153</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="214">214</biblScope><biblScope unit="page" to="226">226</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1994</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The membrane-bound metalloprotease aminopeptidase N (APN, CD13, EC 3.4.11.2) is a well-established marker of normal and malignant cells of the myelomonocytic lineages. It is also expressed by leukemic blasts of a small group of patients suffering from acute or chronic lymphoid leukemia. CD13-specific monoclonal antibodies do not bind to the surface of normal B lymphocytes and APN-mRNA was not detectable by Northern analysis in normal lymphocytes or in T cell lines. A recent paper, however, describes the expression of CD13 on concanavalin A-stimulated T cells. Here, by means of enzymatic amplification of cDNA, cloning and sequencing of amplified cDNA fragments, in situ hybridization, and transcription start site mapping, evidence is provided that the human T cell lines HuT78 and H9 do contain APN-mRNA. Ala-pNA-hydrolyzing activity was detected in viable cells and cell homogenates as well as in size-fractionated protein fractions. Alanine-β-naphthylamide (Ala-βNA)-hydrolyzing activity was readily detectable after isoelectric focusing and blotting and was shown to be localized exclusively intracellularly in both cell lines. Usage of aminopeptidase-specific effectors revealed this activity to be distinct from CD13.</p></abstract></profileDesc><revisionDesc><change when="1994">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-Z3MR9B50-Z/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YCIMM</jid><aid>71019</aid><ce:pii>S0008-8749(84)71019-7</ce:pii><ce:doi>10.1006/cimm.1994.1019</ce:doi><ce:copyright type="full-transfer" year="1994">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Expression of the Aminopeptidase N (CD13) Gene in the Human T Cell Lines HuT78 and H9</ce:title><ce:author-group><ce:author><ce:given-name>U.</ce:given-name><ce:surname>Lendeckel</ce:surname></ce:author><ce:author><ce:given-name>Th.</ce:given-name><ce:surname>Wex</ce:surname></ce:author><ce:author><ce:given-name>Th.</ce:given-name><ce:surname>Kähne</ce:surname></ce:author><ce:author><ce:given-name>K.</ce:given-name><ce:surname>Frank</ce:surname></ce:author><ce:author><ce:given-name>D.</ce:given-name><ce:surname>Reinhold</ce:surname></ce:author><ce:author><ce:given-name>S.</ce:given-name><ce:surname>Ansorge</ce:surname></ce:author><ce:affiliation><ce:textfn>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</ce:textfn></ce:affiliation></ce:author-group><ce:abstract class="author"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0010">The membrane-bound metalloprotease aminopeptidase N (APN, CD13, EC 3.4.11.2) is a well-established marker of normal and malignant cells of the myelomonocytic lineages. It is also expressed by leukemic blasts of a small group of patients suffering from acute or chronic lymphoid leukemia. CD13-specific monoclonal antibodies do not bind to the surface of normal B lymphocytes and APN-mRNA was not detectable by Northern analysis in normal lymphocytes or in T cell lines. A recent paper, however, describes the expression of CD13 on concanavalin A-stimulated T cells. Here, by means of enzymatic amplification of cDNA, cloning and sequencing of amplified cDNA fragments, <ce:italic>in situ</ce:italic> hybridization, and transcription start site mapping, evidence is provided that the human T cell lines HuT78 and H9 do contain APN-mRNA. Ala-pNA-hydrolyzing activity was detected in viable cells and cell homogenates as well as in size-fractionated protein fractions. Alanine-β-naphthylamide (Ala-βNA)-hydrolyzing activity was readily detectable after isoelectric focusing and blotting and was shown to be localized exclusively intracellularly in both cell lines. Usage of aminopeptidase-specific effectors revealed this activity to be distinct from CD13.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Expression of the Aminopeptidase N (CD13) Gene in the Human T Cell Lines HuT78 and H9</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Expression of the Aminopeptidase N (CD13) Gene in the Human T Cell Lines HuT78 and H9</title></titleInfo><name type="personal"><namePart type="given">U.</namePart><namePart type="family">Lendeckel</namePart><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Th.</namePart><namePart type="family">Wex</namePart><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Th.</namePart><namePart type="family">Kähne</namePart><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Frank</namePart><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Reinhold</namePart><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Ansorge</namePart><affiliation>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The membrane-bound metalloprotease aminopeptidase N (APN, CD13, EC 3.4.11.2) is a well-established marker of normal and malignant cells of the myelomonocytic lineages. It is also expressed by leukemic blasts of a small group of patients suffering from acute or chronic lymphoid leukemia. CD13-specific monoclonal antibodies do not bind to the surface of normal B lymphocytes and APN-mRNA was not detectable by Northern analysis in normal lymphocytes or in T cell lines. A recent paper, however, describes the expression of CD13 on concanavalin A-stimulated T cells. Here, by means of enzymatic amplification of cDNA, cloning and sequencing of amplified cDNA fragments, in situ hybridization, and transcription start site mapping, evidence is provided that the human T cell lines HuT78 and H9 do contain APN-mRNA. Ala-pNA-hydrolyzing activity was detected in viable cells and cell homogenates as well as in size-fractionated protein fractions. Alanine-β-naphthylamide (Ala-βNA)-hydrolyzing activity was readily detectable after isoelectric focusing and blotting and was shown to be localized exclusively intracellularly in both cell lines. Usage of aminopeptidase-specific effectors revealed this activity to be distinct from CD13.</abstract><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>Cellular Immunology</title></titleInfo><titleInfo type="abbreviated"><title>YCIMM</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued></originInfo><identifier type="ISSN">0008-8749</identifier><identifier type="PII">S0008-8749(00)X0118-9</identifier><part><date>1994</date><detail type="volume"><number>153</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>276</end></extent><extent unit="pages"><start>214</start><end>226</end></extent></part></relatedItem><identifier type="istex">EEDE35298F7A4A95DA30E2558AD5CFFDA2971763</identifier><identifier type="ark">ark:/67375/6H6-Z3MR9B50-Z</identifier><identifier type="DOI">10.1006/cimm.1994.1019</identifier><identifier type="PII">S0008-8749(84)71019-7</identifier><identifier type="ArticleID">71019</identifier><accessCondition type="use and reproduction" contentType="copyright">©1994 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1994</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-Z3MR9B50-Z/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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