Binding of porcine transmissible gastroenteritis virus by enterocytes from newborn and weaned piglets
Identifieur interne : 000657 ( Istex/Corpus ); précédent : 000656; suivant : 000658Binding of porcine transmissible gastroenteritis virus by enterocytes from newborn and weaned piglets
Auteurs : H. M. Weingartl ; J. B. DerbyshireSource :
- Veterinary Microbiology [ 0378-1135 ] ; 1993.
English descriptors
- Teeft :
- Alkaline phosphatase activity, Assay, Cell surface, Cells preincubated, Chelation, Chelation enterocytes, Competitive virus binding assay, Crypt, Cryptal, Cryptal enterocytes, Derbyshire, Enterocyte, Enterocyte fractions, Enterocytes, Gastroenteritis, Gastroenteritis virus, Intestine, Mdbk, Mdbk cells, Newborn piglet, Newborn piglets, Piglet, Plasma membrane, Porcine, Radiolabelled virus, Receptor, Saturable, Saturable binding, Saturable binding sites, Small intestine, Specific receptors, Tgev, Total binding, Transmissible, Transmissible gastroenteritis, Transmissible gastroenteritis virus, Villous, Villous enterocytes, Villus, Virus, Virus binding.
Abstract
Abstract: Enterocytes were harvested by chelation in a series of seven fractions from the tips of the villi to the crypts of the jejunum of newborn or weaned piglets. Binding of the low cell culture passaged Miller-6 strain of transmissible gastroenteritis virus (TGEV) to villous enterocytes from newborn piglets was at a high level, similar to that observed to culturedswine test is (ST) cells. Binding of the virus to cryptal enterocytes from newborn piglets or to villous or cryptal enterocytes from weaned piglets was significantly lower. In a competitive virus binding assay with radiolabelled virus, the binding of TGEV to ST cells was found to be saturable, while binding to MDBK cells, in which the virus fails to replicate, was at a lower level and was non-saturable. In the same assay, virus binding to the villous enterocytes from the jejunum of a newborn piglet was saturable, while binding to cryptal enterocytes from a newborn piglet, and to villous and cryptal enterocytes from a weaned piglet, was non-saturable. It was concluded that the high susceptibility of newborn piglets to TGEV infection, and the tropism of the virus for villous enterocytes, may relate to the presence of specific, saturable binding sites on the plasma membrane of villous enterocytes.
Url:
DOI: 10.1016/0378-1135(93)90113-L
Links to Exploration step
ISTEX:55F980F38D856E8724DD9C335FE16DB74BBBA562Le document en format XML
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Binding of the low cell culture passaged Miller-6 strain of transmissible gastroenteritis virus (TGEV) to villous enterocytes from newborn piglets was at a high level, similar to that observed to culturedswine test is (ST) cells. Binding of the virus to cryptal enterocytes from newborn piglets or to villous or cryptal enterocytes from weaned piglets was significantly lower. In a competitive virus binding assay with radiolabelled virus, the binding of TGEV to ST cells was found to be saturable, while binding to MDBK cells, in which the virus fails to replicate, was at a lower level and was non-saturable. In the same assay, virus binding to the villous enterocytes from the jejunum of a newborn piglet was saturable, while binding to cryptal enterocytes from a newborn piglet, and to villous and cryptal enterocytes from a weaned piglet, was non-saturable. It was concluded that the high susceptibility of newborn piglets to TGEV infection, and the tropism of the virus for villous enterocytes, may relate to the presence of specific, saturable binding sites on the plasma membrane of villous enterocytes.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>enterocytes</json:string><json:string>piglet</json:string><json:string>tgev</json:string><json:string>receptor</json:string><json:string>gastroenteritis</json:string><json:string>villus</json:string><json:string>newborn piglets</json:string><json:string>villous</json:string><json:string>saturable</json:string><json:string>virus binding</json:string><json:string>chelation</json:string><json:string>villous enterocytes</json:string><json:string>enterocyte</json:string><json:string>derbyshire</json:string><json:string>porcine</json:string><json:string>mdbk</json:string><json:string>saturable binding</json:string><json:string>crypt</json:string><json:string>assay</json:string><json:string>cryptal enterocytes</json:string><json:string>cryptal</json:string><json:string>transmissible</json:string><json:string>transmissible gastroenteritis</json:string><json:string>mdbk cells</json:string><json:string>transmissible gastroenteritis virus</json:string><json:string>newborn piglet</json:string><json:string>gastroenteritis virus</json:string><json:string>total binding</json:string><json:string>intestine</json:string><json:string>small intestine</json:string><json:string>saturable binding sites</json:string><json:string>radiolabelled virus</json:string><json:string>alkaline phosphatase activity</json:string><json:string>plasma membrane</json:string><json:string>specific receptors</json:string><json:string>competitive virus binding assay</json:string><json:string>enterocyte fractions</json:string><json:string>chelation enterocytes</json:string><json:string>cell surface</json:string><json:string>cells preincubated</json:string><json:string>virus</json:string></teeft></keywords><author><json:item><name>H.M. Weingartl</name><affiliations><json:string>Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ont., Canada</json:string></affiliations></json:item><json:item><name>J.B. Derbyshire</name><affiliations><json:string>Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ont., Canada</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-8GPN0MG9-G</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Enterocytes were harvested by chelation in a series of seven fractions from the tips of the villi to the crypts of the jejunum of newborn or weaned piglets. Binding of the low cell culture passaged Miller-6 strain of transmissible gastroenteritis virus (TGEV) to villous enterocytes from newborn piglets was at a high level, similar to that observed to culturedswine test is (ST) cells. Binding of the virus to cryptal enterocytes from newborn piglets or to villous or cryptal enterocytes from weaned piglets was significantly lower. In a competitive virus binding assay with radiolabelled virus, the binding of TGEV to ST cells was found to be saturable, while binding to MDBK cells, in which the virus fails to replicate, was at a lower level and was non-saturable. In the same assay, virus binding to the villous enterocytes from the jejunum of a newborn piglet was saturable, while binding to cryptal enterocytes from a newborn piglet, and to villous and cryptal enterocytes from a weaned piglet, was non-saturable. 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(1992)</json:string><json:string>Saif and Heckert, 1990</json:string><json:string>Yeager et al., 1992</json:string><json:string>Bohl and Pensaert, 1989</json:string><json:string>Vlasak et al., 1988</json:string><json:string>Delmas et al. ( 1992 )</json:string><json:string>Weiser, 1973</json:string><json:string>Witte and Walther, 1976</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-8GPN0MG9-G</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - veterinary sciences</json:string><json:string>2 - microbiology</json:string></wos><scienceMetrix><json:string>1 - applied sciences</json:string><json:string>2 - agriculture, fisheries & forestry</json:string><json:string>3 - veterinary sciences</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Veterinary</json:string><json:string>3 - General Veterinary</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Microbiology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string><json:string>4 - gastroenterologie. foie. pancreas. abdomen</json:string></inist></categories><publicationDate>1993</publicationDate><copyrightDate>1993</copyrightDate><doi><json:string>10.1016/0378-1135(93)90113-L</json:string></doi><id>55F980F38D856E8724DD9C335FE16DB74BBBA562</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-8GPN0MG9-G/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-8GPN0MG9-G/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-8GPN0MG9-G/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Binding of porcine transmissible gastroenteritis virus by enterocytes from newborn and weaned piglets</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1993</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Binding of porcine transmissible gastroenteritis virus by enterocytes from newborn and weaned piglets</title><author xml:id="author-0000"><persName><forename type="first">H.M.</forename><surname>Weingartl</surname></persName><affiliation>Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ont., Canada</affiliation></author><author xml:id="author-0001"><persName><forename type="first">J.B.</forename><surname>Derbyshire</surname></persName><note type="correspondence"><p>Correspondence to: J.B. Derbyshire, Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.</p></note><affiliation>Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ont., Canada</affiliation></author><idno type="istex">55F980F38D856E8724DD9C335FE16DB74BBBA562</idno><idno type="ark">ark:/67375/6H6-8GPN0MG9-G</idno><idno type="DOI">10.1016/0378-1135(93)90113-L</idno><idno type="PII">0378-1135(93)90113-L</idno></analytic><monogr><title level="j">Veterinary Microbiology</title><title level="j" type="abbrev">VETMIC</title><idno type="pISSN">0378-1135</idno><idno type="PII">S0378-1135(00)X0198-8</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993"></date><biblScope unit="volume">35</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="23">23</biblScope><biblScope unit="page" to="32">32</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1993</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Enterocytes were harvested by chelation in a series of seven fractions from the tips of the villi to the crypts of the jejunum of newborn or weaned piglets. Binding of the low cell culture passaged Miller-6 strain of transmissible gastroenteritis virus (TGEV) to villous enterocytes from newborn piglets was at a high level, similar to that observed to culturedswine test is (ST) cells. Binding of the virus to cryptal enterocytes from newborn piglets or to villous or cryptal enterocytes from weaned piglets was significantly lower. In a competitive virus binding assay with radiolabelled virus, the binding of TGEV to ST cells was found to be saturable, while binding to MDBK cells, in which the virus fails to replicate, was at a lower level and was non-saturable. In the same assay, virus binding to the villous enterocytes from the jejunum of a newborn piglet was saturable, while binding to cryptal enterocytes from a newborn piglet, and to villous and cryptal enterocytes from a weaned piglet, was non-saturable. It was concluded that the high susceptibility of newborn piglets to TGEV infection, and the tropism of the virus for villous enterocytes, may relate to the presence of specific, saturable binding sites on the plasma membrane of villous enterocytes.</p></abstract></profileDesc><revisionDesc><change when="1993">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-8GPN0MG9-G/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>VETMIC</jid><aid>9390113L</aid><ce:pii>0378-1135(93)90113-L</ce:pii><ce:doi>10.1016/0378-1135(93)90113-L</ce:doi><ce:copyright type="unknown" year="1993"></ce:copyright></item-info><head><ce:title>Binding of porcine transmissible gastroenteritis virus by enterocytes from newborn and weaned piglets</ce:title><ce:author-group><ce:author><ce:given-name>H.M.</ce:given-name><ce:surname>Weingartl</ce:surname></ce:author><ce:author><ce:given-name>J.B.</ce:given-name><ce:surname>Derbyshire</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ont., Canada</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence to: J.B. Derbyshire, Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="8" month="10" year="1992"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Enterocytes were harvested by chelation in a series of seven fractions from the tips of the villi to the crypts of the jejunum of newborn or weaned piglets. Binding of the low cell culture passaged Miller-6 strain of transmissible gastroenteritis virus (TGEV) to villous enterocytes from newborn piglets was at a high level, similar to that observed to culturedswine test is (ST) cells. Binding of the virus to cryptal enterocytes from newborn piglets or to villous or cryptal enterocytes from weaned piglets was significantly lower. In a competitive virus binding assay with radiolabelled virus, the binding of TGEV to ST cells was found to be saturable, while binding to MDBK cells, in which the virus fails to replicate, was at a lower level and was non-saturable. In the same assay, virus binding to the villous enterocytes from the jejunum of a newborn piglet was saturable, while binding to cryptal enterocytes from a newborn piglet, and to villous and cryptal enterocytes from a weaned piglet, was non-saturable. It was concluded that the high susceptibility of newborn piglets to TGEV infection, and the tropism of the virus for villous enterocytes, may relate to the presence of specific, saturable binding sites on the plasma membrane of villous enterocytes.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Binding of porcine transmissible gastroenteritis virus by enterocytes from newborn and weaned piglets</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Binding of porcine transmissible gastroenteritis virus by enterocytes from newborn and weaned piglets</title></titleInfo><name type="personal"><namePart type="given">H.M.</namePart><namePart type="family">Weingartl</namePart><affiliation>Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ont., Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.B.</namePart><namePart type="family">Derbyshire</namePart><affiliation>Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ont., Canada</affiliation><description>Correspondence to: J.B. Derbyshire, Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Enterocytes were harvested by chelation in a series of seven fractions from the tips of the villi to the crypts of the jejunum of newborn or weaned piglets. Binding of the low cell culture passaged Miller-6 strain of transmissible gastroenteritis virus (TGEV) to villous enterocytes from newborn piglets was at a high level, similar to that observed to culturedswine test is (ST) cells. Binding of the virus to cryptal enterocytes from newborn piglets or to villous or cryptal enterocytes from weaned piglets was significantly lower. In a competitive virus binding assay with radiolabelled virus, the binding of TGEV to ST cells was found to be saturable, while binding to MDBK cells, in which the virus fails to replicate, was at a lower level and was non-saturable. In the same assay, virus binding to the villous enterocytes from the jejunum of a newborn piglet was saturable, while binding to cryptal enterocytes from a newborn piglet, and to villous and cryptal enterocytes from a weaned piglet, was non-saturable. It was concluded that the high susceptibility of newborn piglets to TGEV infection, and the tropism of the virus for villous enterocytes, may relate to the presence of specific, saturable binding sites on the plasma membrane of villous enterocytes.</abstract><relatedItem type="host"><titleInfo><title>Veterinary Microbiology</title></titleInfo><titleInfo type="abbreviated"><title>VETMIC</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued></originInfo><identifier type="ISSN">0378-1135</identifier><identifier type="PII">S0378-1135(00)X0198-8</identifier><part><date>1993</date><detail type="volume"><number>35</number><caption>vol.</caption></detail><detail type="issue"><number>1–2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>185</end></extent><extent unit="pages"><start>23</start><end>32</end></extent></part></relatedItem><identifier type="istex">55F980F38D856E8724DD9C335FE16DB74BBBA562</identifier><identifier type="ark">ark:/67375/6H6-8GPN0MG9-G</identifier><identifier type="DOI">10.1016/0378-1135(93)90113-L</identifier><identifier type="PII">0378-1135(93)90113-L</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-8GPN0MG9-G/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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