Heterogeneity of infectious bronchitis virus grown in eggs
Identifieur interne : 000584 ( Istex/Corpus ); précédent : 000583; suivant : 000585Heterogeneity of infectious bronchitis virus grown in eggs
Auteurs : M. S. Collins ; D. J. Alexander ; J. W. HarknessSource :
- Archives of Virology [ 0304-8608 ] ; 1976-03-01.
English descriptors
- Teeft :
- Academic press, Allantoic fluid, Amorphous material, Avian, Bronchitis, Bronchitis virus, Buoyant density, Cellular material, Complement fixation test, Continuous sucrose gradients, Densest peak, Density gradient peaks, Different preparations, Discontinuous density gradients, Discontinuous gradient, Distinct peaks, Electron microscopy, Fertile chicken eggs, Fraction number, Great britain, Heterogeneity, Infectious bronchitis virus, Infectivity, Isopyenie separation, Large particles, Lipid composition, Major polypeptides, Minor polypeptides, Newcastle disease virus, Other peaks, Peak, Pleomorphic virus particles, Polypeptide, Relative migration, Similar results, Strain beaudette, Sucrose, Sucrose density gradients, Sucrose solutions, Virol, Virology, Virus, Virus particles.
Abstract
Summary: Egg-grown infectious bronchitis virus, strain Beaudette, was concentrated and centrifuged on sucrose density gradients to separate the virus into five peaks with densities of 1.144, 1.160, 1.172, 1.191 and 1.218 g/cm3. All peaks retained infectivity, complement fixation activity and were labelled with3H-uridine. Morphologically the densest peak consisted of very large virus particles and amorphous material, the other peaks consisted of mainly intact particles although small differences in size and pleomorphism were seen. Polyacrylamide gel electrophoresis of material from the density gradient peaks revealed four major polypeptides and at least 10 minor polypeptides. The proportions of the polypeptides were approximately similar for all peaks with the exception of the densest peak in which the major polypeptides were greatly reduced. The four major polypeptides had approximate molecular weights of 1. 52,000, 2. 45,000, 3. 34,000, 4. 32,000. The major polypeptides 1 and 4 were shown to be glycosylated as were two of the minor polypeptides.
Url:
DOI: 10.1007/BF01318001
Links to Exploration step
ISTEX:8CD998C8F1C58A9441BDE584ACD3AD6647937DC1Le document en format XML
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Collins</name><affiliation><mods:affiliation>Poultry Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, Great Britain</mods:affiliation></affiliation><affiliation><mods:affiliation>Virology Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, Great Britain</mods:affiliation></affiliation></author><author><name sortKey="Alexander, D J" sort="Alexander, D J" uniqKey="Alexander D" first="D. J." last="Alexander">D. J. Alexander</name><affiliation><mods:affiliation>Poultry Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, Great Britain</mods:affiliation></affiliation><affiliation><mods:affiliation>Virology Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, Great Britain</mods:affiliation></affiliation></author><author><name sortKey="Harkness, J W" sort="Harkness, J W" uniqKey="Harkness J" first="J. W." last="Harkness">J. W. 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All peaks retained infectivity, complement fixation activity and were labelled with3H-uridine. Morphologically the densest peak consisted of very large virus particles and amorphous material, the other peaks consisted of mainly intact particles although small differences in size and pleomorphism were seen. Polyacrylamide gel electrophoresis of material from the density gradient peaks revealed four major polypeptides and at least 10 minor polypeptides. The proportions of the polypeptides were approximately similar for all peaks with the exception of the densest peak in which the major polypeptides were greatly reduced. The four major polypeptides had approximate molecular weights of 1. 52,000, 2. 45,000, 3. 34,000, 4. 32,000. 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Polyacrylamide gel electrophoresis of material from the density gradient peaks revealed four major polypeptides and at least 10 minor polypeptides. The proportions of the polypeptides were approximately similar for all peaks with the exception of the densest peak in which the major polypeptides were greatly reduced. The four major polypeptides had approximate molecular weights of 1. 52,000, 2. 45,000, 3. 34,000, 4. 32,000. 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All peaks retained infectivity, complement fixation activity and were labelled with3H-uridine. Morphologically the densest peak consisted of very large virus particles and amorphous material, the other peaks consisted of mainly intact particles although small differences in size and pleomorphism were seen. Polyacrylamide gel electrophoresis of material from the density gradient peaks revealed four major polypeptides and at least 10 minor polypeptides. The proportions of the polypeptides were approximately similar for all peaks with the exception of the densest peak in which the major polypeptides were greatly reduced. The four major polypeptides had approximate molecular weights of 1. 52,000, 2. 45,000, 3. 34,000, 4. 32,000. The major polypeptides 1 and 4 were shown to be glycosylated as were two of the minor polypeptides.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Medical Microbiology</term></item><item><term>Virology</term></item><item><term>Infectious Diseases</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1975-04-24">Created</change><change when="1976-03-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-CZ5J5V1V-S/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" 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TocLevels="0"><VolumeIDStart>50</VolumeIDStart><VolumeIDEnd>50</VolumeIDEnd><VolumeIssueCount>4</VolumeIssueCount></VolumeInfo><Issue IssueType="Combined"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>2</IssueIDEnd><IssueArticleCount>17</IssueArticleCount><IssueHistory><CoverDate><DateString>1976</DateString><Year>1976</Year><Month>3</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1976</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art6"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF01318001</ArticleID><ArticleDOI>10.1007/BF01318001</ArticleDOI><ArticleSequenceNumber>6</ArticleSequenceNumber><ArticleTitle Language="En">Heterogeneity of infectious bronchitis virus grown in eggs</ArticleTitle><ArticleCategory>Original Papers</ArticleCategory><ArticleFirstPage>55</ArticleFirstPage><ArticleLastPage>72</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>3</Month><Day>2</Day></RegistrationDate><Received><Year>1975</Year><Month>4</Month><Day>24</Day></Received><Accepted><Year>1975</Year><Month>9</Month><Day>3</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1976</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>705</JournalID><VolumeIDStart>50</VolumeIDStart><VolumeIDEnd>50</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>2</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>M.</GivenName><GivenName>S.</GivenName><FamilyName>Collins</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>D.</GivenName><GivenName>J.</GivenName><FamilyName>Alexander</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>J.</GivenName><GivenName>W.</GivenName><FamilyName>Harkness</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Poultry Department</OrgDivision><OrgName>Central Veterinary Laboratory</OrgName><OrgAddress><City>New Haw, Weybridge</City><State>Surrey</State><Country>Great Britain</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Virology Department</OrgDivision><OrgName>Central Veterinary Laboratory</OrgName><OrgAddress><City>New Haw, Weybridge</City><State>Surrey</State><Country>Great Britain</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Summary</Heading><Para>Egg-grown infectious bronchitis virus, strain Beaudette, was concentrated and centrifuged on sucrose density gradients to separate the virus into five peaks with densities of 1.144, 1.160, 1.172, 1.191 and 1.218 g/cm<Superscript>3</Superscript>. All peaks retained infectivity, complement fixation activity and were labelled with<Superscript>3</Superscript>H-uridine. Morphologically the densest peak consisted of very large virus particles and amorphous material, the other peaks consisted of mainly intact particles although small differences in size and pleomorphism were seen.</Para><Para>Polyacrylamide gel electrophoresis of material from the density gradient peaks revealed four major polypeptides and at least 10 minor polypeptides. The proportions of the polypeptides were approximately similar for all peaks with the exception of the densest peak in which the major polypeptides were greatly reduced. The four major polypeptides had approximate molecular weights of 1. 52,000, 2. 45,000, 3. 34,000, 4. 32,000. The major polypeptides 1 and 4 were shown to be glycosylated as were two of the minor polypeptides.</Para></Abstract><ArticleNote Type="Misc"><SimplePara>With 8 Figures</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Heterogeneity of infectious bronchitis virus grown in eggs</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Heterogeneity of infectious bronchitis virus grown in eggs</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="given">S.</namePart><namePart type="family">Collins</namePart><affiliation>Poultry Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, Great Britain</affiliation><affiliation>Virology Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, Great Britain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="given">J.</namePart><namePart type="family">Alexander</namePart><affiliation>Poultry Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, Great Britain</affiliation><affiliation>Virology Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, Great Britain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="given">W.</namePart><namePart type="family">Harkness</namePart><affiliation>Virology Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, Great Britain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Vienna</placeTerm></place><dateCreated encoding="w3cdtf">1975-04-24</dateCreated><dateIssued encoding="w3cdtf">1976-03-01</dateIssued><copyrightDate encoding="w3cdtf">1976</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Summary: Egg-grown infectious bronchitis virus, strain Beaudette, was concentrated and centrifuged on sucrose density gradients to separate the virus into five peaks with densities of 1.144, 1.160, 1.172, 1.191 and 1.218 g/cm3. All peaks retained infectivity, complement fixation activity and were labelled with3H-uridine. Morphologically the densest peak consisted of very large virus particles and amorphous material, the other peaks consisted of mainly intact particles although small differences in size and pleomorphism were seen. Polyacrylamide gel electrophoresis of material from the density gradient peaks revealed four major polypeptides and at least 10 minor polypeptides. The proportions of the polypeptides were approximately similar for all peaks with the exception of the densest peak in which the major polypeptides were greatly reduced. The four major polypeptides had approximate molecular weights of 1. 52,000, 2. 45,000, 3. 34,000, 4. 32,000. The major polypeptides 1 and 4 were shown to be glycosylated as were two of the minor polypeptides.</abstract><note>Original Papers</note><relatedItem type="host"><titleInfo><title>Archives of Virology</title></titleInfo><titleInfo type="abbreviated"><title>Archives of Virology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1976-03-01</dateIssued><copyrightDate encoding="w3cdtf">1976</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Virology</topic><topic>Infectious Diseases</topic></subject><identifier type="ISSN">0304-8608</identifier><identifier type="eISSN">1432-8798</identifier><identifier type="JournalID">705</identifier><identifier type="IssueArticleCount">17</identifier><identifier type="VolumeIssueCount">4</identifier><part><date>1976</date><detail type="volume"><number>50</number><caption>vol.</caption></detail><detail type="issue"><number>1-2</number><caption>no.</caption></detail><extent unit="pages"><start>55</start><end>72</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1976</recordOrigin></recordInfo></relatedItem><identifier type="istex">8CD998C8F1C58A9441BDE584ACD3AD6647937DC1</identifier><identifier type="ark">ark:/67375/1BB-CZ5J5V1V-S</identifier><identifier type="DOI">10.1007/BF01318001</identifier><identifier type="ArticleID">BF01318001</identifier><identifier type="ArticleID">Art6</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1976</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 1976</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-CZ5J5V1V-S/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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