Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures
Identifieur interne : 000553 ( Istex/Corpus ); précédent : 000552; suivant : 000554Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures
Auteurs : Christin Munkebye Aarnes ; Inger Helene Madshus ; Jean Claude Guillemot ; Kirsten Sandvig ; Sjur OlsnesSource :
- Experimental Cell Research [ 0014-4827 ] ; 1987.
English descriptors
- Teeft :
- Ammonium acetate, Apical side, Asialoglycoprotein receptor, Biotin, Biotinylated, Biotinylated cells, Buoyant density, Cell surface, Cellular receptor, Cona, Conformational change, Conjugate, Diphtheria toxin, Free streptavidin, Hela, Hela ohio cells, Hydrophobic domains, Intact conjugate, Large number, Lysate, Microtitre plates, Natural poliovirus receptors, Olsnes, Poliovirus, Poliovirus receptors, Proc natl acad, Productive infection, Radioactive material, Receptor, Same buffer, Sandvig, Sendai virus, Specific binding, Streptavidin, Unbound conjugate, Unmodified, Unmodified cells, Unmodified virus, Unreacted streptavidin, Unreacted virus, Virus, Virus entry, Virus particle, Virus particles, Virus receptor, Virus surface.
Abstract
Abstract: Disulfide-linked conjugates of poliovirus with streptavidin or concanavalin A were formed and the binding of the conjugates to mouse L cells that lack natural poliovirus receptors was studied. The conjugate with streptavidin was specifically bound to biotinylated L cells, but not to unmodified L cells. The conjugate with conA was bound to L cells in the absence of, but not in the presence of α-methyl mannoside. Incubation of L cells with bound conjugates did not produce virus, although the conjugates were highly infectious in HeLa cells, containing natural poliovirus receptors. This suggests that the artificially bound virus was unable to penetrate the L cells and start replication. The possibility that binding of the virus to the natural receptor is required for efficient infection is discussed.
Url:
DOI: 10.1016/0014-4827(87)90322-3
Links to Exploration step
ISTEX:3B27B2F33D7D4109ACB94A21E6B005653E03FA5ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures</title>
<author><name sortKey="Aarnes, Christin Munkebye" sort="Aarnes, Christin Munkebye" uniqKey="Aarnes C" first="Christin Munkebye" last="Aarnes">Christin Munkebye Aarnes</name>
<affiliation><mods:affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Madshus, Inger Helene" sort="Madshus, Inger Helene" uniqKey="Madshus I" first="Inger Helene" last="Madshus">Inger Helene Madshus</name>
<affiliation><mods:affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Guillemot, Jean Claude" sort="Guillemot, Jean Claude" uniqKey="Guillemot J" first="Jean Claude" last="Guillemot">Jean Claude Guillemot</name>
<affiliation><mods:affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Sandvig, Kirsten" sort="Sandvig, Kirsten" uniqKey="Sandvig K" first="Kirsten" last="Sandvig">Kirsten Sandvig</name>
<affiliation><mods:affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Olsnes, Sjur" sort="Olsnes, Sjur" uniqKey="Olsnes S" first="Sjur" last="Olsnes">Sjur Olsnes</name>
<affiliation><mods:affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:3B27B2F33D7D4109ACB94A21E6B005653E03FA5A</idno>
<date when="1987" year="1987">1987</date>
<idno type="doi">10.1016/0014-4827(87)90322-3</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-BVM124HB-M/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000553</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000553</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures</title>
<author><name sortKey="Aarnes, Christin Munkebye" sort="Aarnes, Christin Munkebye" uniqKey="Aarnes C" first="Christin Munkebye" last="Aarnes">Christin Munkebye Aarnes</name>
<affiliation><mods:affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Madshus, Inger Helene" sort="Madshus, Inger Helene" uniqKey="Madshus I" first="Inger Helene" last="Madshus">Inger Helene Madshus</name>
<affiliation><mods:affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Guillemot, Jean Claude" sort="Guillemot, Jean Claude" uniqKey="Guillemot J" first="Jean Claude" last="Guillemot">Jean Claude Guillemot</name>
<affiliation><mods:affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Sandvig, Kirsten" sort="Sandvig, Kirsten" uniqKey="Sandvig K" first="Kirsten" last="Sandvig">Kirsten Sandvig</name>
<affiliation><mods:affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Olsnes, Sjur" sort="Olsnes, Sjur" uniqKey="Olsnes S" first="Sjur" last="Olsnes">Sjur Olsnes</name>
<affiliation><mods:affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Experimental Cell Research</title>
<title level="j" type="abbrev">YEXCR</title>
<idno type="ISSN">0014-4827</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1987">1987</date>
<biblScope unit="volume">170</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="483">483</biblScope>
<biblScope unit="page" to="490">490</biblScope>
</imprint>
<idno type="ISSN">0014-4827</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0014-4827</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Ammonium acetate</term>
<term>Apical side</term>
<term>Asialoglycoprotein receptor</term>
<term>Biotin</term>
<term>Biotinylated</term>
<term>Biotinylated cells</term>
<term>Buoyant density</term>
<term>Cell surface</term>
<term>Cellular receptor</term>
<term>Cona</term>
<term>Conformational change</term>
<term>Conjugate</term>
<term>Diphtheria toxin</term>
<term>Free streptavidin</term>
<term>Hela</term>
<term>Hela ohio cells</term>
<term>Hydrophobic domains</term>
<term>Intact conjugate</term>
<term>Large number</term>
<term>Lysate</term>
<term>Microtitre plates</term>
<term>Natural poliovirus receptors</term>
<term>Olsnes</term>
<term>Poliovirus</term>
<term>Poliovirus receptors</term>
<term>Proc natl acad</term>
<term>Productive infection</term>
<term>Radioactive material</term>
<term>Receptor</term>
<term>Same buffer</term>
<term>Sandvig</term>
<term>Sendai virus</term>
<term>Specific binding</term>
<term>Streptavidin</term>
<term>Unbound conjugate</term>
<term>Unmodified</term>
<term>Unmodified cells</term>
<term>Unmodified virus</term>
<term>Unreacted streptavidin</term>
<term>Unreacted virus</term>
<term>Virus</term>
<term>Virus entry</term>
<term>Virus particle</term>
<term>Virus particles</term>
<term>Virus receptor</term>
<term>Virus surface</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Disulfide-linked conjugates of poliovirus with streptavidin or concanavalin A were formed and the binding of the conjugates to mouse L cells that lack natural poliovirus receptors was studied. The conjugate with streptavidin was specifically bound to biotinylated L cells, but not to unmodified L cells. The conjugate with conA was bound to L cells in the absence of, but not in the presence of α-methyl mannoside. Incubation of L cells with bound conjugates did not produce virus, although the conjugates were highly infectious in HeLa cells, containing natural poliovirus receptors. This suggests that the artificially bound virus was unable to penetrate the L cells and start replication. The possibility that binding of the virus to the natural receptor is required for efficient infection is discussed.</div>
</front>
</TEI>
<istex><corpusName>elsevier</corpusName>
<keywords><teeft><json:string>poliovirus</json:string>
<json:string>streptavidin</json:string>
<json:string>receptor</json:string>
<json:string>cona</json:string>
<json:string>hela</json:string>
<json:string>biotinylated</json:string>
<json:string>biotin</json:string>
<json:string>sandvig</json:string>
<json:string>olsnes</json:string>
<json:string>lysate</json:string>
<json:string>hela ohio cells</json:string>
<json:string>hydrophobic domains</json:string>
<json:string>same buffer</json:string>
<json:string>virus particles</json:string>
<json:string>unmodified</json:string>
<json:string>virus</json:string>
<json:string>proc natl acad</json:string>
<json:string>biotinylated cells</json:string>
<json:string>apical side</json:string>
<json:string>conjugate</json:string>
<json:string>cellular receptor</json:string>
<json:string>conformational change</json:string>
<json:string>diphtheria toxin</json:string>
<json:string>productive infection</json:string>
<json:string>virus particle</json:string>
<json:string>specific binding</json:string>
<json:string>microtitre plates</json:string>
<json:string>cell surface</json:string>
<json:string>unreacted virus</json:string>
<json:string>free streptavidin</json:string>
<json:string>ammonium acetate</json:string>
<json:string>unreacted streptavidin</json:string>
<json:string>virus surface</json:string>
<json:string>radioactive material</json:string>
<json:string>buoyant density</json:string>
<json:string>large number</json:string>
<json:string>virus receptor</json:string>
<json:string>unbound conjugate</json:string>
<json:string>natural poliovirus receptors</json:string>
<json:string>unmodified cells</json:string>
<json:string>intact conjugate</json:string>
<json:string>virus entry</json:string>
<json:string>asialoglycoprotein receptor</json:string>
<json:string>sendai virus</json:string>
<json:string>poliovirus receptors</json:string>
<json:string>unmodified virus</json:string>
</teeft>
</keywords>
<author><json:item><name>Christin Munkebye Aarnes</name>
<affiliations><json:string>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</json:string>
</affiliations>
</json:item>
<json:item><name>Inger Helene Madshus</name>
<affiliations><json:string>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</json:string>
</affiliations>
</json:item>
<json:item><name>Jean Claude Guillemot</name>
<affiliations><json:string>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</json:string>
</affiliations>
</json:item>
<json:item><name>Kirsten Sandvig</name>
<affiliations><json:string>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</json:string>
</affiliations>
</json:item>
<json:item><name>Sjur Olsnes</name>
<affiliations><json:string>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</json:string>
</affiliations>
</json:item>
</author>
<arkIstex>ark:/67375/6H6-BVM124HB-M</arkIstex>
<language><json:string>eng</json:string>
</language>
<originalGenre><json:string>Full-length article</json:string>
</originalGenre>
<abstract>Abstract: Disulfide-linked conjugates of poliovirus with streptavidin or concanavalin A were formed and the binding of the conjugates to mouse L cells that lack natural poliovirus receptors was studied. The conjugate with streptavidin was specifically bound to biotinylated L cells, but not to unmodified L cells. The conjugate with conA was bound to L cells in the absence of, but not in the presence of α-methyl mannoside. Incubation of L cells with bound conjugates did not produce virus, although the conjugates were highly infectious in HeLa cells, containing natural poliovirus receptors. This suggests that the artificially bound virus was unable to penetrate the L cells and start replication. The possibility that binding of the virus to the natural receptor is required for efficient infection is discussed.</abstract>
<qualityIndicators><score>6.92</score>
<pdfWordCount>3408</pdfWordCount>
<pdfCharCount>19276</pdfCharCount>
<pdfVersion>1.3</pdfVersion>
<pdfPageCount>8</pdfPageCount>
<pdfPageSize>468 x 684 pts</pdfPageSize>
<refBibsNative>true</refBibsNative>
<abstractWordCount>126</abstractWordCount>
<abstractCharCount>817</abstractCharCount>
<keywordCount>0</keywordCount>
</qualityIndicators>
<title>Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures</title>
<pmid><json:string>3036553</json:string>
</pmid>
<pii><json:string>0014-4827(87)90322-3</json:string>
</pii>
<genre><json:string>research-article</json:string>
</genre>
<host><title>Experimental Cell Research</title>
<language><json:string>unknown</json:string>
</language>
<publicationDate>1987</publicationDate>
<issn><json:string>0014-4827</json:string>
</issn>
<pii><json:string>S0014-4827(00)X0486-7</json:string>
</pii>
<volume>170</volume>
<issue>2</issue>
<pages><first>483</first>
<last>490</last>
</pages>
<genre><json:string>journal</json:string>
</genre>
</host>
<namedEntities><unitex><date><json:string>1987</json:string>
<json:string>35S</json:string>
</date>
<geogName></geogName>
<orgName><json:string>National Institute for Public Health, Oslo, Norway</json:string>
<json:string>Norway The Norwegian Cancer Society, Disulfide</json:string>
<json:string>Sigma Chemical Co.</json:string>
<json:string>Academic Press, Inc.</json:string>
</orgName>
<orgName_funder></orgName_funder>
<orgName_provider></orgName_provider>
<persName></persName>
<placeName><json:string>Sweden</json:string>
</placeName>
<ref_url></ref_url>
<ref_bibl><json:string>[23]</json:string>
<json:string>Aarnes et al.</json:string>
<json:string>[6]</json:string>
<json:string>[22]</json:string>
<json:string>Carlsson et al. 1161</json:string>
<json:string>[17]</json:string>
<json:string>Fuller et al. [5]</json:string>
<json:string>[3]</json:string>
<json:string>[3,4]</json:string>
<json:string>[14]</json:string>
<json:string>[24]</json:string>
</ref_bibl>
<bibl></bibl>
</unitex>
</namedEntities>
<ark><json:string>ark:/67375/6H6-BVM124HB-M</json:string>
</ark>
<categories><wos><json:string>1 - science</json:string>
<json:string>2 - oncology</json:string>
<json:string>2 - cell biology</json:string>
</wos>
<scienceMetrix><json:string>1 - health sciences</json:string>
<json:string>2 - biomedical research</json:string>
<json:string>3 - biochemistry & molecular biology</json:string>
</scienceMetrix>
<scopus><json:string>1 - Life Sciences</json:string>
<json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string>
<json:string>3 - Cell Biology</json:string>
</scopus>
<inist><json:string>1 - sciences appliquees, technologies et medecines</json:string>
<json:string>2 - sciences biologiques et medicales</json:string>
<json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string>
</inist>
</categories>
<publicationDate>1987</publicationDate>
<copyrightDate>1987</copyrightDate>
<doi><json:string>10.1016/0014-4827(87)90322-3</json:string>
</doi>
<id>3B27B2F33D7D4109ACB94A21E6B005653E03FA5A</id>
<score>1</score>
<fulltext><json:item><extension>pdf</extension>
<original>true</original>
<mimetype>application/pdf</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-BVM124HB-M/fulltext.pdf</uri>
</json:item>
<json:item><extension>zip</extension>
<original>false</original>
<mimetype>application/zip</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-BVM124HB-M/bundle.zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-BVM124HB-M/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures</title>
</titleStmt>
<publicationStmt><authority>ISTEX</authority>
<publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher>
<availability><licence><p>elsevier</p>
</licence>
</availability>
<p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p>
<date>1987</date>
</publicationStmt>
<notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note>
<note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note>
</notesStmt>
<sourceDesc><biblStruct type="inbook"><analytic><title level="a">Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures</title>
<author xml:id="author-0000"><persName><forename type="first">Christin Munkebye</forename>
<surname>Aarnes</surname>
</persName>
<note type="biography">C. M. A. had a fellowship from Norges Almenvitenskapelige Forskningsråd, Oslo.</note>
<affiliation>C. M. A. had a fellowship from Norges Almenvitenskapelige Forskningsråd, Oslo.</affiliation>
<affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</affiliation>
</author>
<author xml:id="author-0001"><persName><forename type="first">Inger Helene</forename>
<surname>Madshus</surname>
</persName>
<affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</affiliation>
</author>
<author xml:id="author-0002"><persName><forename type="first">Jean Claude</forename>
<surname>Guillemot</surname>
</persName>
<affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</affiliation>
</author>
<author xml:id="author-0003"><persName><forename type="first">Kirsten</forename>
<surname>Sandvig</surname>
</persName>
<affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</affiliation>
</author>
<author xml:id="author-0004"><persName><forename type="first">Sjur</forename>
<surname>Olsnes</surname>
</persName>
<note type="biography">To whom offprint requests should be sent.</note>
<affiliation>To whom offprint requests should be sent.</affiliation>
<affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</affiliation>
</author>
<idno type="istex">3B27B2F33D7D4109ACB94A21E6B005653E03FA5A</idno>
<idno type="ark">ark:/67375/6H6-BVM124HB-M</idno>
<idno type="DOI">10.1016/0014-4827(87)90322-3</idno>
<idno type="PII">0014-4827(87)90322-3</idno>
</analytic>
<monogr><title level="j">Experimental Cell Research</title>
<title level="j" type="abbrev">YEXCR</title>
<idno type="pISSN">0014-4827</idno>
<idno type="PII">S0014-4827(00)X0486-7</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1987"></date>
<biblScope unit="volume">170</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="483">483</biblScope>
<biblScope unit="page" to="490">490</biblScope>
</imprint>
</monogr>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><creation><date>1987</date>
</creation>
<langUsage><language ident="en">en</language>
</langUsage>
<abstract xml:lang="en"><p>Abstract: Disulfide-linked conjugates of poliovirus with streptavidin or concanavalin A were formed and the binding of the conjugates to mouse L cells that lack natural poliovirus receptors was studied. The conjugate with streptavidin was specifically bound to biotinylated L cells, but not to unmodified L cells. The conjugate with conA was bound to L cells in the absence of, but not in the presence of α-methyl mannoside. Incubation of L cells with bound conjugates did not produce virus, although the conjugates were highly infectious in HeLa cells, containing natural poliovirus receptors. This suggests that the artificially bound virus was unable to penetrate the L cells and start replication. The possibility that binding of the virus to the natural receptor is required for efficient infection is discussed.</p>
</abstract>
</profileDesc>
<revisionDesc><change when="1987">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
<json:item><extension>txt</extension>
<original>false</original>
<mimetype>text/plain</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-BVM124HB-M/fulltext.txt</uri>
</json:item>
</fulltext>
<metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration>
<istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType>
<istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YEXCR</jid>
<aid>87903223</aid>
<ce:pii>0014-4827(87)90322-3</ce:pii>
<ce:doi>10.1016/0014-4827(87)90322-3</ce:doi>
<ce:copyright type="unknown" year="1987"></ce:copyright>
</item-info>
<head><ce:title>Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures</ce:title>
<ce:author-group><ce:author><ce:given-name>Christin Munkebye</ce:given-name>
<ce:surname>Aarnes</ce:surname>
<ce:cross-ref refid="FN1"><ce:sup>1</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Inger Helene</ce:given-name>
<ce:surname>Madshus</ce:surname>
</ce:author>
<ce:author><ce:given-name>Jean Claude</ce:given-name>
<ce:surname>Guillemot</ce:surname>
</ce:author>
<ce:author><ce:given-name>Kirsten</ce:given-name>
<ce:surname>Sandvig</ce:surname>
</ce:author>
<ce:author><ce:given-name>Sjur</ce:given-name>
<ce:surname>Olsnes</ce:surname>
<ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:affiliation><ce:textfn>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</ce:textfn>
</ce:affiliation>
<ce:correspondence id="COR1"><ce:label>∗</ce:label>
<ce:text>To whom offprint requests should be sent.</ce:text>
</ce:correspondence>
<ce:footnote id="FN1"><ce:label>1</ce:label>
<ce:note-para>C. M. A. had a fellowship from Norges Almenvitenskapelige Forskningsråd, Oslo.</ce:note-para>
</ce:footnote>
</ce:author-group>
<ce:date-received day="26" month="11" year="1986"></ce:date-received>
<ce:abstract><ce:section-title>Abstract</ce:section-title>
<ce:abstract-sec><ce:simple-para>Disulfide-linked conjugates of poliovirus with streptavidin or concanavalin A were formed and the binding of the conjugates to mouse L cells that lack natural poliovirus receptors was studied. The conjugate with streptavidin was specifically bound to biotinylated L cells, but not to unmodified L cells. The conjugate with conA was bound to L cells in the absence of, but not in the presence of α-methyl mannoside. Incubation of L cells with bound conjugates did not produce virus, although the conjugates were highly infectious in HeLa cells, containing natural poliovirus receptors. This suggests that the artificially bound virus was unable to penetrate the L cells and start replication. The possibility that binding of the virus to the natural receptor is required for efficient infection is discussed.</ce:simple-para>
</ce:abstract-sec>
</ce:abstract>
</head>
</converted-article>
</istex:document>
</istex:metadataXml>
<mods version="3.6"><titleInfo><title>Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA"><title>Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures</title>
</titleInfo>
<name type="personal"><namePart type="given">Christin Munkebye</namePart>
<namePart type="family">Aarnes</namePart>
<affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</affiliation>
<description>C. M. A. had a fellowship from Norges Almenvitenskapelige Forskningsråd, Oslo.</description>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Inger Helene</namePart>
<namePart type="family">Madshus</namePart>
<affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Jean Claude</namePart>
<namePart type="family">Guillemot</namePart>
<affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Kirsten</namePart>
<namePart type="family">Sandvig</namePart>
<affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Sjur</namePart>
<namePart type="family">Olsnes</namePart>
<affiliation>Institute for Cancer Research, The Norwegian Radium Hospital, The Norwegian Cancer Society, Montebello, Oslo 3, Norway</affiliation>
<description>To whom offprint requests should be sent.</description>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre>
<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1987</dateIssued>
<copyrightDate encoding="w3cdtf">1987</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="iso639-2b">eng</languageTerm>
<languageTerm type="code" authority="rfc3066">en</languageTerm>
</language>
<abstract lang="en">Abstract: Disulfide-linked conjugates of poliovirus with streptavidin or concanavalin A were formed and the binding of the conjugates to mouse L cells that lack natural poliovirus receptors was studied. The conjugate with streptavidin was specifically bound to biotinylated L cells, but not to unmodified L cells. The conjugate with conA was bound to L cells in the absence of, but not in the presence of α-methyl mannoside. Incubation of L cells with bound conjugates did not produce virus, although the conjugates were highly infectious in HeLa cells, containing natural poliovirus receptors. This suggests that the artificially bound virus was unable to penetrate the L cells and start replication. The possibility that binding of the virus to the natural receptor is required for efficient infection is discussed.</abstract>
<relatedItem type="host"><titleInfo><title>Experimental Cell Research</title>
</titleInfo>
<titleInfo type="abbreviated"><title>YEXCR</title>
</titleInfo>
<genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre>
<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1987</dateIssued>
</originInfo>
<identifier type="ISSN">0014-4827</identifier>
<identifier type="PII">S0014-4827(00)X0486-7</identifier>
<part><date>1987</date>
<detail type="volume"><number>170</number>
<caption>vol.</caption>
</detail>
<detail type="issue"><number>2</number>
<caption>no.</caption>
</detail>
<extent unit="issue-pages"><start>259</start>
<end>512</end>
</extent>
<extent unit="pages"><start>483</start>
<end>490</end>
</extent>
</part>
</relatedItem>
<identifier type="istex">3B27B2F33D7D4109ACB94A21E6B005653E03FA5A</identifier>
<identifier type="ark">ark:/67375/6H6-BVM124HB-M</identifier>
<identifier type="DOI">10.1016/0014-4827(87)90322-3</identifier>
<identifier type="PII">0014-4827(87)90322-3</identifier>
<recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource>
</recordInfo>
</mods>
<json:item><extension>json</extension>
<original>false</original>
<mimetype>application/json</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-BVM124HB-M/record.json</uri>
</json:item>
</metadata>
<serie></serie>
</istex>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000553 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000553 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV1 |flux= Istex |étape= Corpus |type= RBID |clé= ISTEX:3B27B2F33D7D4109ACB94A21E6B005653E03FA5A |texte= Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |