TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions
Identifieur interne : 000464 ( Istex/Corpus ); précédent : 000463; suivant : 000465TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions
Auteurs : Murielle Godet ; Rene L'Haridon ; Jean-Francois Vautherot ; Hubert LaudeSource :
- Virology [ 0042-6822 ] ; 1992.
English descriptors
- Teeft :
- Academic press, Acnpv, Acorf4, Amino, Amino acid sequence, Amino acid sequences, Amino acids, Ascites fluids, Assay, Baculovirus, Bronchitis virus, Cell extracts, Cell monolayers, Clone, Common feature, Consensus sequence, Core sequence, Coronavirus, Coronavirus genome, Coronavirus genomes, Coronavirus members, Coronavirus study group, Coronaviruses, Encode nonstructural proteins, Epitope, Eukaryotic proteins, Gastroenteritis, Gastroenteritis virus, Genome, Godet, Human coronavirus, Hybridoma, Hydrophobic polypeptide, Immunofluorescence, Immunoprecipitated, Integral membrane protein, Laude, Mabs, Mammalian cells, Matrix protein genes, Membrane protein, Molar ratio, Monoclonal, Monoclonal antibodies, Mrna, Nonreducing conditions, Nucleotide sequence, Open reading frame, Orf4, Orf4 polypeptide, Orf4 product, Orf4 protein, Orf4 sequence, Orfs, Other coronaviruses, Peptide, Peptide scanning, Plasmid, Polyclonal antibodies, Polypeptide, Preliminary experiments, Reading frames, Recombinant, Recombinant baculovirus, Recombinant baculoviruses, Recombinant orf4 protein, Sample buffer, Sequence analysis, Simian virus, Spike glycoprotein, Structural polypeptide, Surface fluorescence, Tgev, Tgev orf4, Tgev particles, Tgev virions, Transmembrane orientation, Transmissible gastroenteritis virus, Unique regions, Viral, Virion, Virology, Virus, Wild type acnpv.
Abstract
Abstract: The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence. Two epitopes were localized within the last 21 C-terminal residues of the sequence through peptide scanning and analysis of the reactivity of a truncated ORF4 recombinant protein. Since the relevant MAbs were found to induce a cell surface fluorescence, these data suggest that ORF4 may be an integral membrane protein having a Cexo-Nendo orientation. Anti-ORF4 MAbs were also used to show that ORF4 polypeptide may be detected in TGEV virion preparations, with an estimated number of 20 molecules incorporated per particle. Comparison of amino acid sequence data provided strong evidence that other coronaviruses encode a polypeptide homologous to TGEV ORF4. Our results led us to propose that ORF4 represents a novel minor structural polypeptide, tentatively designated SM (small membrane protein).
Url:
DOI: 10.1016/0042-6822(92)90521-P
Links to Exploration step
ISTEX:80743413DA1F1D29E7BBC0E2FD258B0C3BF66216Le document en format XML
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last="Vautherot">Jean-Francois Vautherot</name><affiliation><mods:affiliation>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses, France</mods:affiliation></affiliation></author><author><name sortKey="Laude, Hubert" sort="Laude, Hubert" uniqKey="Laude H" first="Hubert" last="Laude">Hubert Laude</name><affiliation><mods:affiliation>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">188</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="666">666</biblScope><biblScope unit="page" to="675">675</biblScope></imprint><idno 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baculoviruses</term><term>Recombinant orf4 protein</term><term>Sample buffer</term><term>Sequence analysis</term><term>Simian virus</term><term>Spike glycoprotein</term><term>Structural polypeptide</term><term>Surface fluorescence</term><term>Tgev</term><term>Tgev orf4</term><term>Tgev particles</term><term>Tgev virions</term><term>Transmembrane orientation</term><term>Transmissible gastroenteritis virus</term><term>Unique regions</term><term>Viral</term><term>Virion</term><term>Virology</term><term>Virus</term><term>Wild type acnpv</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence. Two epitopes were localized within the last 21 C-terminal residues of the sequence through peptide scanning and analysis of the reactivity of a truncated ORF4 recombinant protein. Since the relevant MAbs were found to induce a cell surface fluorescence, these data suggest that ORF4 may be an integral membrane protein having a Cexo-Nendo orientation. Anti-ORF4 MAbs were also used to show that ORF4 polypeptide may be detected in TGEV virion preparations, with an estimated number of 20 molecules incorporated per particle. Comparison of amino acid sequence data provided strong evidence that other coronaviruses encode a polypeptide homologous to TGEV ORF4. 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Agronomique, 78350 Jouy-en-Joses, France</json:string></affiliations></json:item><json:item><name>Jean-Francois Vautherot</name><affiliations><json:string>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses, France</json:string></affiliations></json:item><json:item><name>Hubert Laude</name><affiliations><json:string>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses, France</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-1KH3HVD1-V</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence. Two epitopes were localized within the last 21 C-terminal residues of the sequence through peptide scanning and analysis of the reactivity of a truncated ORF4 recombinant protein. Since the relevant MAbs were found to induce a cell surface fluorescence, these data suggest that ORF4 may be an integral membrane protein having a Cexo-Nendo orientation. Anti-ORF4 MAbs were also used to show that ORF4 polypeptide may be detected in TGEV virion preparations, with an estimated number of 20 molecules incorporated per particle. Comparison of amino acid sequence data provided strong evidence that other coronaviruses encode a polypeptide homologous to TGEV ORF4. Our results led us to propose that ORF4 represents a novel minor structural polypeptide, tentatively designated SM (small membrane protein).</abstract><qualityIndicators><score>9.352</score><pdfWordCount>5784</pdfWordCount><pdfCharCount>36836</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>196</abstractWordCount><abstractCharCount>1353</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions</title><pmid><json:string>1316677</json:string></pmid><pii><json:string>0042-6822(92)90521-P</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci. USA</title><language><json:string>unknown</json:string></language><volume>81</volume><pages><first>3998</first><last>4002</last></pages></serie><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1992</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0433-3</json:string></pii><volume>188</volume><issue>2</issue><pages><first>666</first><last>675</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1992</json:string><json:string>35S</json:string></date><geogName><json:string>Semliki</json:string></geogName><orgName><json:string>Cambridge Research Biochemicals</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>D. X. Liu</json:string><json:string>J. F. Vautherot</json:string><json:string>P. Boireau</json:string><json:string>J. Levin</json:string><json:string>J. Gelfi</json:string><json:string>M. Nezondb</json:string></persName><placeName><json:string>Mannheim</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Rasschaert et al., 1987</json:string><json:string>Welch and Sefton, 1980</json:string><json:string>Haridon et al., 1991</json:string><json:string>Garwes et al., 1989</json:string><json:string>Raabe and Siddel, 1989</json:string><json:string>Kiefhaber et al., 1991</json:string><json:string>Brown and Faulkner (1977)</json:string><json:string>Surgrue and Hay, 1991</json:string><json:string>Boursnell et al., 1985</json:string><json:string>Budzilowicz and Weiss, 1987</json:string><json:string>Delmas and Laude, 1991</json:string><json:string>Kapke and Brian, 1986</json:string><json:string>Hay et al., 1985</json:string><json:string>Britton et al., 1989</json:string><json:string>Skinner et al., 1985</json:string><json:string>Zebedee and Lamb, 1988</json:string><json:string>Summers and Smith (1987)</json:string><json:string>Gaedigk-Nitschko and Schlesinger, 1990</json:string><json:string>Geysen et al., 1984</json:string><json:string>Delmas and Laude, 1990</json:string><json:string>Schmidt, 1982</json:string><json:string>Laude et al., 1986</json:string><json:string>Liu et al., 1991</json:string><json:string>Britton et al., 1988</json:string><json:string>Smith et al., 1990</json:string><json:string>Wesley et al., 1989</json:string><json:string>Olmsted and Collins, 1989</json:string><json:string>Lamb et al., 1985</json:string><json:string>Luckow and Summers, 1989</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-1KH3HVD1-V</json:string></ark><categories><wos><json:string>1 - 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Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence. Two epitopes were localized within the last 21 C-terminal residues of the sequence through peptide scanning and analysis of the reactivity of a truncated ORF4 recombinant protein. Since the relevant MAbs were found to induce a cell surface fluorescence, these data suggest that ORF4 may be an integral membrane protein having a Cexo-Nendo orientation. Anti-ORF4 MAbs were also used to show that ORF4 polypeptide may be detected in TGEV virion preparations, with an estimated number of 20 molecules incorporated per particle. Comparison of amino acid sequence data provided strong evidence that other coronaviruses encode a polypeptide homologous to TGEV ORF4. Our results led us to propose that ORF4 represents a novel minor structural polypeptide, tentatively designated SM (small membrane protein).</p></abstract></profileDesc><revisionDesc><change when="1992">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-1KH3HVD1-V/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>9290521P</aid><ce:pii>0042-6822(92)90521-P</ce:pii><ce:doi>10.1016/0042-6822(92)90521-P</ce:doi><ce:copyright type="unknown" year="1992"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular article</ce:textfn></ce:dochead><ce:title>TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions</ce:title><ce:author-group><ce:author><ce:given-name>Murielle</ce:given-name><ce:surname>Godet</ce:surname></ce:author><ce:author><ce:given-name>Rene</ce:given-name><ce:surname>L'Haridon</ce:surname></ce:author><ce:author><ce:given-name>Jean-Francois</ce:given-name><ce:surname>Vautherot</ce:surname></ce:author><ce:author><ce:given-name>Hubert</ce:given-name><ce:surname>Laude</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom correspondence and reprint requests should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="18" month="11" year="1991"></ce:date-received><ce:date-accepted day="26" month="2" year="1992"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence. Two epitopes were localized within the last 21 C-terminal residues of the sequence through peptide scanning and analysis of the reactivity of a truncated ORF4 recombinant protein. Since the relevant MAbs were found to induce a cell surface fluorescence, these data suggest that ORF4 may be an integral membrane protein having a Cexo-Nendo orientation. Anti-ORF4 MAbs were also used to show that ORF4 polypeptide may be detected in TGEV virion preparations, with an estimated number of 20 molecules incorporated per particle. Comparison of amino acid sequence data provided strong evidence that other coronaviruses encode a polypeptide homologous to TGEV ORF4. Our results led us to propose that ORF4 represents a novel minor structural polypeptide, tentatively designated SM (small membrane protein).</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions</title></titleInfo><name type="personal"><namePart type="given">Murielle</namePart><namePart type="family">Godet</namePart><affiliation>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rene</namePart><namePart type="family">L'Haridon</namePart><affiliation>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean-Francois</namePart><namePart type="family">Vautherot</namePart><affiliation>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hubert</namePart><namePart type="family">Laude</namePart><affiliation>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses, France</affiliation><description>To whom correspondence and reprint requests should be addressed.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence. Two epitopes were localized within the last 21 C-terminal residues of the sequence through peptide scanning and analysis of the reactivity of a truncated ORF4 recombinant protein. Since the relevant MAbs were found to induce a cell surface fluorescence, these data suggest that ORF4 may be an integral membrane protein having a Cexo-Nendo orientation. Anti-ORF4 MAbs were also used to show that ORF4 polypeptide may be detected in TGEV virion preparations, with an estimated number of 20 molecules incorporated per particle. Comparison of amino acid sequence data provided strong evidence that other coronaviruses encode a polypeptide homologous to TGEV ORF4. Our results led us to propose that ORF4 represents a novel minor structural polypeptide, tentatively designated SM (small membrane protein).</abstract><note type="content">Section title: Regular article</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0433-3</identifier><part><date>1992</date><detail type="volume"><number>188</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>417</start><end>975</end></extent><extent unit="pages"><start>666</start><end>675</end></extent></part></relatedItem><identifier type="istex">80743413DA1F1D29E7BBC0E2FD258B0C3BF66216</identifier><identifier type="ark">ark:/67375/6H6-1KH3HVD1-V</identifier><identifier type="DOI">10.1016/0042-6822(92)90521-P</identifier><identifier type="PII">0042-6822(92)90521-P</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-1KH3HVD1-V/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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