A Pocket‐Sized Convective PCR Thermocycler
Identifieur interne : 000403 ( Istex/Corpus ); précédent : 000402; suivant : 000404A Pocket‐Sized Convective PCR Thermocycler
Auteurs : Nitin Agrawal ; Yassin Hassan ; Victor UgazSource :
- Angewandte Chemie International Edition [ 1433-7851 ] ; 2007-06-04.
Abstract
Is that a PCR in your pocket? An innovative thermocycling system is presented that harnesses natural convection to perform rapid multiplex and long‐target DNA amplification by the polymerase chain reaction (PCR). The design is inherently simple and consumes minimal electrical power, enabling a pocket‐sized battery‐powered device to be constructed at a cost of approximately US$10.
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DOI: 10.1002/anie.200700306
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ISTEX:886609DEAE41C083E3F32CEF77C3AA0E2BED8C43Le document en format XML
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An innovative thermocycling system is presented that harnesses natural convection to perform rapid multiplex and long‐target DNA amplification by the polymerase chain reaction (PCR). 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KGaA, Weinheim</copyright><eventGroup><event type="manuscriptReceived" date="2007-01-23"></event><event type="firstOnline" date="2007-04-30"></event><event type="publishedOnlineFinalForm" date="2007-05-24"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.6 mode:FullText source:FullText result:FullText" date="2010-04-20"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-03"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-14"></event></eventGroup><numberingGroup><numbering type="pageFirst">4316</numbering><numbering type="pageLast">4319</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:ANIE.ANIE200700306.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="0"></count><count type="referenceTotal" number="17"></count></countGroup><titleGroup><title type="main" xml:lang="en">A Pocket‐Sized Convective PCR Thermocycler<link href="#nss"></link></title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#aa #ap"><personName><givenNames>Nitin</givenNames><familyName>Agrawal</familyName><degrees>Dr.</degrees></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#ab"><personName><givenNames>Yassin A.</givenNames><familyName>Hassan</familyName><degrees>Prof.</degrees></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#aa"><personName><givenNames>Victor M.</givenNames><familyName>Ugaz</familyName><degrees>Prof.</degrees></personName><contactDetails><email>ugaz@tamu.edu</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="aa" countryCode="US" type="organization"><unparsedAffiliation>Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA, Fax: (+1) 979‐458‐1002</unparsedAffiliation></affiliation><affiliation xml:id="ab" countryCode="US" type="organization"><unparsedAffiliation>Department of Nuclear Engineering, Texas A&M University, College Station, TX 77843, USA</unparsedAffiliation></affiliation><affiliation xml:id="ap" countryCode="US" type="organization"><unparsedAffiliation>Present address: Center for Engineering in Medicine/Department of Surgery, Massachusetts General Hospital, Shriners Burns Hospital and Harvard Medical School, Boston, MA 02114, USA</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">analytical methods</keyword><keyword xml:id="kwd2">DNA replication</keyword><keyword xml:id="kwd3">microreactors</keyword><keyword xml:id="kwd4">molecular biology</keyword><keyword xml:id="kwd5">polymerase chain reaction</keyword></keywordGroup><fundingInfo><fundingAgency>National Institutes of Health</fundingAgency><fundingNumber>K22-HG02297</fundingNumber><fundingNumber>R01-HG003364</fundingNumber></fundingInfo><supportingInformation><p>Supporting information for this article is available on the WWW under <url href="http://www.wiley-vch.de/contents/jc_2002/2007/z700306_s.pdf">http://www.wiley‐vch.de/contents/jc_2002/2007/z700306_s.pdf</url> or from the author.</p></supportingInformation><abstractGroup><abstract type="graphical" xml:lang="en"><p><b>Is that a PCR in your pocket?</b> An innovative thermocycling system is presented that harnesses natural convection to perform rapid multiplex and long‐target DNA amplification by the polymerase chain reaction (PCR). The design is inherently simple and consumes minimal electrical power, enabling a pocket‐sized battery‐powered device to be constructed at a cost of approximately US$10.<blockFixed type="graphic"><mediaResourceGroup><mediaResource alt="image" eRights="yes" copyright="WILEY-VCH" href="urn:x-wiley:14337851:media:ANIE200700306:content"></mediaResource><mediaResource alt="thumbnail image" rendition="webLoRes" mimeType="image/gif" href=""></mediaResource><mediaResource alt="original image" rendition="webOriginal" mimeType="image/gif" href=""></mediaResource><mediaResource alt="magnified image" rendition="webHiRes" mimeType="image/gif" href=""></mediaResource></mediaResourceGroup></blockFixed></p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="nss"><p>This work was supported by the National Institutes of Health under grants K22‐HG02297 and R01‐HG003364. We thank Prof. Mihir Sen for helpful discussions regarding modeling the convective flow. We are also grateful to Ling Zhen for assistance with computational‐flow simulations.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>A Pocket‐Sized Convective PCR Thermocycler</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>A Pocket‐Sized Convective PCR Thermocycler</title></titleInfo><name type="personal"><namePart type="given">Nitin</namePart><namePart type="family">Agrawal</namePart><namePart type="termsOfAddress">Dr.</namePart><affiliation>Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA, Fax: (+1) 979‐458‐1002</affiliation><affiliation>Present address: Center for Engineering in Medicine/Department of Surgery, Massachusetts General Hospital, Shriners Burns Hospital and Harvard Medical School, Boston, MA 02114, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yassin A.</namePart><namePart type="family">Hassan</namePart><namePart type="termsOfAddress">Prof.</namePart><affiliation>Department of Nuclear Engineering, Texas A&M University, College Station, TX 77843, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Victor M.</namePart><namePart type="family">Ugaz</namePart><namePart type="termsOfAddress">Prof.</namePart><affiliation>Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA, Fax: (+1) 979‐458‐1002</affiliation><affiliation>E-mail: ugaz@tamu.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="shortCommunication" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</genre><originInfo><publisher>WILEY‐VCH Verlag</publisher><place><placeTerm type="text">Weinheim</placeTerm></place><dateIssued encoding="w3cdtf">2007-06-04</dateIssued><dateCaptured encoding="w3cdtf">2007-01-23</dateCaptured><copyrightDate encoding="w3cdtf">2007</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">4</extent><extent unit="tables">0</extent><extent unit="references">17</extent></physicalDescription><abstract type="graphical" lang="en">Is that a PCR in your pocket? An innovative thermocycling system is presented that harnesses natural convection to perform rapid multiplex and long‐target DNA amplification by the polymerase chain reaction (PCR). The design is inherently simple and consumes minimal electrical power, enabling a pocket‐sized battery‐powered device to be constructed at a cost of approximately US$10.</abstract><note type="content">*This work was supported by the National Institutes of Health under grants K22‐HG02297 and R01‐HG003364. We thank Prof. Mihir Sen for helpful discussions regarding modeling the convective flow. We are also grateful to Ling Zhen for assistance with computational‐flow simulations.</note><note type="funding">National Institutes of Health - No. K22-HG02297; No. R01-HG003364; </note><subject lang="en"><genre>keywords</genre><topic>analytical methods</topic><topic>DNA replication</topic><topic>microreactors</topic><topic>molecular biology</topic><topic>polymerase chain reaction</topic></subject><relatedItem type="host"><titleInfo><title>Angewandte Chemie International Edition</title></titleInfo><titleInfo type="abbreviated"><title>Angewandte Chemie International Edition</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><note type="content"> Supporting information for this article is available on the WWW under http://www.wiley‐vch.de/contents/jc_2002/2007/z700306_s.pdf or from the author.</note><subject><genre>article-category</genre><topic>Communication</topic><topic>Communications</topic></subject><identifier type="ISSN">1433-7851</identifier><identifier type="eISSN">1521-3773</identifier><identifier type="DOI">10.1002/(ISSN)1521-3773</identifier><identifier type="PublisherID">ANIE</identifier><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>46</number></detail><detail type="issue"><caption>no.</caption><number>23</number></detail><extent unit="pages"><start>4316</start><end>4319</end><total>4</total></extent></part></relatedItem><relatedItem type="references" displayLabel="cit1"><titleInfo><title>Nature</title></titleInfo><genre>journal-article</genre><note type="citation/reference">D. Butler, Nature 2006, 440, 6.</note><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Butler</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>440</number></detail><extent unit="pages"><start>6</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit2"><titleInfo><title>Science</title></titleInfo><genre>journal-article</genre><note type="citation/reference">D. A. King, C. Peckham, J. K. Waage, J. Brownlie, M. E. J. Woolhouse, Science 2006, 313, 1392.</note><name type="personal"><namePart type="given">D. A.</namePart><namePart type="family">King</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Peckham</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. K.</namePart><namePart type="family">Waage</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Brownlie</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. E. J.</namePart><namePart type="family">Woolhouse</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>313</number></detail><extent unit="pages"><start>1392</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit3"><titleInfo><title>Nature</title></titleInfo><genre>journal-article</genre><note type="citation/reference">P. Yager, T. Edwards, E. Fu, K. Helton, K. Nelson, M. Tam, B. H. Weigl, Nature 2006, 442, 412.</note><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Yager</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Edwards</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Fu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Helton</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Nelson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Tam</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B. H.</namePart><namePart type="family">Weigl</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>442</number></detail><extent unit="pages"><start>412</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit4"><titleInfo><title>Lab Chip</title></titleInfo><genre>journal-article</genre><note type="citation/reference">C. D. Chin, V. Linder, S. K. Sia, Lab Chip 2007, 7, 41.</note><name type="personal"><namePart type="given">C. D.</namePart><namePart type="family">Chin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Linder</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. K.</namePart><namePart type="family">Sia</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>7</number></detail><extent unit="pages"><start>41</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit5"><titleInfo><title>Lancet Infect. Dis.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">S. Yang, R. E. Rothman, Lancet Infect. Dis. 2004, 4, 337.</note><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Yang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. E.</namePart><namePart type="family">Rothman</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>4</number></detail><extent unit="pages"><start>337</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit6"><titleInfo><title>Nat. Rev. Genet.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">S. Sauer, B. M. H. Lange, J. Gobom, L. Nyarsik, H. Seitz, H. Lehrach, Nat. Rev. Genet. 2005, 6, 465.</note><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Sauer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B. M. H.</namePart><namePart type="family">Lange</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Gobom</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Nyarsik</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Seitz</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Lehrach</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>6</number></detail><extent unit="pages"><start>465</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit7"><titleInfo><title>Nat. Genet.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">A.‐C. Syvänen, Nat. Genet. 2005, 37, S5.</note><name type="personal"><namePart type="given">A.‐C.</namePart><namePart type="family">Syvänen</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>37</number></detail><extent unit="pages"><start>5</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit8"><titleInfo><title>Science</title></titleInfo><genre>journal-article</genre><note type="citation/reference">M. Krishnan, V. M. Ugaz, M. A. Burns, Science 2002, 298, 793.</note><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Krishnan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V. M.</namePart><namePart type="family">Ugaz</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. A.</namePart><namePart type="family">Burns</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>298</number></detail><extent unit="pages"><start>793</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit9"><titleInfo><title>Anal. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">M. Krishnan, N. Agrawal, M. A. Burns, V. M. Ugaz, Anal. Chem. 2004, 76, 6254.</note><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Krishnan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N.</namePart><namePart type="family">Agrawal</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. A.</namePart><namePart type="family">Burns</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V. M.</namePart><namePart type="family">Ugaz</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>76</number></detail><extent unit="pages"><start>6254</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit10"><titleInfo><title>Europhys. Lett.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">E. Yariv, G. Ben‐Dov, K. D. Dorfman, Europhys. Lett. 2005, 71, 1008.</note><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Yariv</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Ben‐Dov</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K. D.</namePart><namePart type="family">Dorfman</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>71</number></detail><extent unit="pages"><start>1008</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit11"><titleInfo><title>J. Assoc. Lab. Autom.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">N. Agrawal, V. M. Ugaz, J. Assoc. Lab. Autom. 2006, 11, 217.</note><name type="personal"><namePart type="given">N.</namePart><namePart type="family">Agrawal</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V. M.</namePart><namePart type="family">Ugaz</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>11</number></detail><extent unit="pages"><start>217</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit12"><titleInfo><title>Phys. Rev. Lett.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">D. Braun, N. L. Goddard, A. Libchaber, Phys. Rev. Lett. 2003, 91, 158103.</note><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Braun</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N. L.</namePart><namePart type="family">Goddard</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Libchaber</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>91</number></detail><extent unit="pages"><start>158103</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit13"><titleInfo><title>Anal. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">E. K. Wheeler, W. Benett, P. Stratton, J. Richards, A. Chen, A. Christian, K. D. Ness, J. Ortega, L. G. Li, T. H. Weisgraber, K. Goodson, F. Milanovich, Anal. Chem. 2004, 76, 4011.</note><name type="personal"><namePart type="given">E. K.</namePart><namePart type="family">Wheeler</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Benett</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Stratton</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Richards</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Chen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Christian</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K. D.</namePart><namePart type="family">Ness</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Ortega</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L. G.</namePart><namePart type="family">Li</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T. H.</namePart><namePart type="family">Weisgraber</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Goodson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Milanovich</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>76</number></detail><extent unit="pages"><start>4011</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit14"><titleInfo><title>Anal. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Z. Chen, S. Qian, W. R. Abrams, D. Malamud, H. Bau, Anal. Chem. 2004, 76, 3707.</note><name type="personal"><namePart type="given">Z.</namePart><namePart type="family">Chen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Qian</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W. R.</namePart><namePart type="family">Abrams</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Malamud</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Bau</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>76</number></detail><extent unit="pages"><start>3707</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit15"><titleInfo><title>Appl. Phys. Lett.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">M. Hennig, D. Braun, Appl. Phys. Lett. 2005, 87, 183901.</note><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Hennig</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Braun</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>87</number></detail><extent unit="pages"><start>183901</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit16"><titleInfo><title>Anal. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">M. G. Roper, C. J. Easley, J. P. Landers, Anal. Chem. 2005, 77, 3887.</note><name type="personal"><namePart type="given">M. G.</namePart><namePart type="family">Roper</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. J.</namePart><namePart type="family">Easley</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. P.</namePart><namePart type="family">Landers</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>77</number></detail><extent unit="pages"><start>3887</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit17"><titleInfo><title>10</title></titleInfo><name type="personal"><namePart type="given">K. D.</namePart><namePart type="family">Spitzack</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V. M.</namePart><namePart type="family">Ugaz</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>book-chapter</genre><relatedItem type="host"><titleInfo><title>Microfluidic Techniques: Reviews and Protocols, Vol. 321</title></titleInfo><originInfo><publisher>Humana Press</publisher><place><placeTerm type="text">Totowa, NJ</placeTerm></place></originInfo><part><date>2005</date></part></relatedItem></relatedItem><identifier type="istex">886609DEAE41C083E3F32CEF77C3AA0E2BED8C43</identifier><identifier type="ark">ark:/67375/WNG-M8KH6MSB-X</identifier><identifier type="DOI">10.1002/anie.200700306</identifier><identifier type="ArticleID">ANIE200700306</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2007 WILEY‐VCH Verlag GmbH & Co. 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