Genomic organization and expression of the 3′ end of the canine and feline enteric coronaviruses
Identifieur interne : 000352 ( Istex/Corpus ); précédent : 000351; suivant : 000353Genomic organization and expression of the 3′ end of the canine and feline enteric coronaviruses
Auteurs : H. Vennema ; J. W. A. Rossen ; J. Wesseling ; M. C. Horzinek ; P. J. M. RottierSource :
- Virology [ 0042-6822 ] ; 1992.
English descriptors
- Teeft :
- Amino, Amino acid sequences, Antigenic, Cdna, Cdna clones, Cdna synthesis, Clone, Coronavirus, Coronaviruses, Deletion, Endo, Expression product, Fecv, Feline, Feline coronavirus, Feline coronaviruses, Feline enteric coronavirus, Fipv, Fipv genome, Gastroenteritis, Genome, Genomic, Genomic organization, Glycoprotein, Groot, Nucleotide, Nucleotide sequence, Nucleotide sequences, Orfs, Peritonitis, Peritonitis virus, Polymerase, Primer, Protein bands, Protein genes, Reading frame, Reading frames, Recombinant, Recombinant vaccinia virus, Sequence analysis, Sequence data, Spaan, Structural proteins, Synthetic oligonucleotide, Tgev, Transmissible gastroenteritis virus, Vaccinia, Vennema, Viral, Virology.
Abstract
Abstract: The genomic organization at the 3′ end of canine coronavirus (CCV) and feline enteric coronavirus (FECV) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (FIPV) and transmissible gastroenteritis virus (TGEV) of swine. Comparison of the latter two has previously revealed an extra open reading frame (ORF) at the 3′ end of the FIPV genome, lacking in TGEV, which is currently designated ORF 6b. Both CCV and FECV possess 6b-related ORFs at the 3′ ends of their genomes. The presence of ORF 6b in three of four viruses in this antigenic cluster strongly suggests that TGEV has lost this ORF by deletion. The CCV ORF 6b is collinear with that of FIPV, but the predicted amino acid sequences are only 58% identical. The FECV ORF 6b contains a large deletion compared to that of FIPV, reducing the collinear part to 60%. The sequence homologies were highest between CCV and TGEV on the one hand and between FECV and FIPV on the other. Previously, we showed that the expression product of the FIPV ORF 6b can be detected in infected cells by immunoprecipitation (Vennema et al., 1992). In the present study we have performed similar experiments with CCV and FECV. In infected cells both viruses produced proteins related to but different from the FIPV 6b protein.
Url:
DOI: 10.1016/0042-6822(92)90174-N
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ISTEX:9F3B89832434E67F6645DAC582FAD5BC2A84258DLe document en format XML
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Horzinek</name><affiliation><mods:affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Rottier, P J M" sort="Rottier, P J M" uniqKey="Rottier P" first="P. J. M." last="Rottier">P. J. M. Rottier</name><affiliation><mods:affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">191</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="134">134</biblScope><biblScope unit="page" to="140">140</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid sequences</term><term>Antigenic</term><term>Cdna</term><term>Cdna clones</term><term>Cdna synthesis</term><term>Clone</term><term>Coronavirus</term><term>Coronaviruses</term><term>Deletion</term><term>Endo</term><term>Expression product</term><term>Fecv</term><term>Feline</term><term>Feline coronavirus</term><term>Feline coronaviruses</term><term>Feline enteric coronavirus</term><term>Fipv</term><term>Fipv genome</term><term>Gastroenteritis</term><term>Genome</term><term>Genomic</term><term>Genomic organization</term><term>Glycoprotein</term><term>Groot</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Nucleotide sequences</term><term>Orfs</term><term>Peritonitis</term><term>Peritonitis virus</term><term>Polymerase</term><term>Primer</term><term>Protein bands</term><term>Protein genes</term><term>Reading frame</term><term>Reading frames</term><term>Recombinant</term><term>Recombinant vaccinia virus</term><term>Sequence analysis</term><term>Sequence data</term><term>Spaan</term><term>Structural proteins</term><term>Synthetic oligonucleotide</term><term>Tgev</term><term>Transmissible gastroenteritis virus</term><term>Vaccinia</term><term>Vennema</term><term>Viral</term><term>Virology</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The genomic organization at the 3′ end of canine coronavirus (CCV) and feline enteric coronavirus (FECV) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (FIPV) and transmissible gastroenteritis virus (TGEV) of swine. Comparison of the latter two has previously revealed an extra open reading frame (ORF) at the 3′ end of the FIPV genome, lacking in TGEV, which is currently designated ORF 6b. Both CCV and FECV possess 6b-related ORFs at the 3′ ends of their genomes. The presence of ORF 6b in three of four viruses in this antigenic cluster strongly suggests that TGEV has lost this ORF by deletion. The CCV ORF 6b is collinear with that of FIPV, but the predicted amino acid sequences are only 58% identical. The FECV ORF 6b contains a large deletion compared to that of FIPV, reducing the collinear part to 60%. The sequence homologies were highest between CCV and TGEV on the one hand and between FECV and FIPV on the other. Previously, we showed that the expression product of the FIPV ORF 6b can be detected in infected cells by immunoprecipitation (Vennema et al., 1992). In the present study we have performed similar experiments with CCV and FECV. In infected cells both viruses produced proteins related to but different from the FIPV 6b protein.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>fipv</json:string><json:string>fecv</json:string><json:string>tgev</json:string><json:string>coronavirus</json:string><json:string>cdna</json:string><json:string>vennema</json:string><json:string>clone</json:string><json:string>genome</json:string><json:string>genomic</json:string><json:string>deletion</json:string><json:string>primer</json:string><json:string>amino acid sequences</json:string><json:string>viral</json:string><json:string>recombinant</json:string><json:string>sequence analysis</json:string><json:string>orfs</json:string><json:string>peritonitis</json:string><json:string>coronaviruses</json:string><json:string>genomic organization</json:string><json:string>gastroenteritis</json:string><json:string>vaccinia</json:string><json:string>virology</json:string><json:string>spaan</json:string><json:string>endo</json:string><json:string>antigenic</json:string><json:string>polymerase</json:string><json:string>glycoprotein</json:string><json:string>amino</json:string><json:string>peritonitis virus</json:string><json:string>nucleotide sequence</json:string><json:string>nucleotide</json:string><json:string>feline</json:string><json:string>groot</json:string><json:string>transmissible gastroenteritis virus</json:string><json:string>recombinant vaccinia virus</json:string><json:string>cdna synthesis</json:string><json:string>cdna clones</json:string><json:string>nucleotide sequences</json:string><json:string>feline coronaviruses</json:string><json:string>feline coronavirus</json:string><json:string>expression product</json:string><json:string>fipv genome</json:string><json:string>feline enteric coronavirus</json:string><json:string>synthetic oligonucleotide</json:string><json:string>structural proteins</json:string><json:string>protein genes</json:string><json:string>sequence data</json:string><json:string>reading frame</json:string><json:string>protein bands</json:string><json:string>reading frames</json:string></teeft></keywords><author><json:item><name>H. Vennema</name><affiliations><json:string>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</json:string></affiliations></json:item><json:item><name>J.W.A. Rossen</name><affiliations><json:string>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</json:string></affiliations></json:item><json:item><name>J. Wesseling</name><affiliations><json:string>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</json:string></affiliations></json:item><json:item><name>M.C. Horzinek</name><affiliations><json:string>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</json:string></affiliations></json:item><json:item><name>P.J.M. Rottier</name><affiliations><json:string>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-FLS1HPF0-X</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The genomic organization at the 3′ end of canine coronavirus (CCV) and feline enteric coronavirus (FECV) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (FIPV) and transmissible gastroenteritis virus (TGEV) of swine. Comparison of the latter two has previously revealed an extra open reading frame (ORF) at the 3′ end of the FIPV genome, lacking in TGEV, which is currently designated ORF 6b. Both CCV and FECV possess 6b-related ORFs at the 3′ ends of their genomes. The presence of ORF 6b in three of four viruses in this antigenic cluster strongly suggests that TGEV has lost this ORF by deletion. The CCV ORF 6b is collinear with that of FIPV, but the predicted amino acid sequences are only 58% identical. The FECV ORF 6b contains a large deletion compared to that of FIPV, reducing the collinear part to 60%. The sequence homologies were highest between CCV and TGEV on the one hand and between FECV and FIPV on the other. Previously, we showed that the expression product of the FIPV ORF 6b can be detected in infected cells by immunoprecipitation (Vennema et al., 1992). In the present study we have performed similar experiments with CCV and FECV. In infected cells both viruses produced proteins related to but different from the FIPV 6b protein.</abstract><qualityIndicators><score>8.887</score><pdfWordCount>4235</pdfWordCount><pdfCharCount>29340</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>7</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>221</abstractWordCount><abstractCharCount>1314</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Genomic organization and expression of the 3′ end of the canine and feline enteric coronaviruses</title><pmid><json:string>1329312</json:string></pmid><pii><json:string>0042-6822(92)90174-N</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. 78th Annu. Meeting U.S. Anim. 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Flare</json:string><json:string>Willy Spaan</json:string><json:string>La Monica</json:string><json:string>B. Moss</json:string><json:string>The</json:string><json:string>To</json:string><json:string>E. Lewis</json:string><json:string>Raoul de Groot</json:string><json:string>N. C. Pedersen</json:string></persName><placeName><json:string>Mannheim</json:string><json:string>Netherlands</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Cavanagh et al., 1990</json:string><json:string>Siddel et al., 1983</json:string><json:string>Rasschaert et al., 1987</json:string><json:string>Binn et al., 1975</json:string><json:string>Garwes et al., 1989</json:string><json:string>Jacobs et al., 1987</json:string><json:string>Fiscus and Teramoto, 1987</json:string><json:string>Tupper et al., 1987</json:string><json:string>Kapke and Brian, 1986</json:string><json:string>Yokomori and Lai, 1991</json:string><json:string>Wesseling et al.</json:string><json:string>Rasschaert et al., 1990</json:string><json:string>Wesley et al., 1990</json:string><json:string>Laemmli, 1970</json:string><json:string>Vennema et al., 1992</json:string><json:string>Lai, 1992</json:string><json:string>Chomczynski and Sacchi, 1987</json:string><json:string>Hohdatsu et al., 1991</json:string><json:string>Parker et al., 1989</json:string><json:string>Kawasaki and Wang, 1989</json:string><json:string>de Groot et al., 1988</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-FLS1HPF0-X</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - microbiologie</json:string></inist></categories><publicationDate>1992</publicationDate><copyrightDate>1992</copyrightDate><doi><json:string>10.1016/0042-6822(92)90174-N</json:string></doi><id>9F3B89832434E67F6645DAC582FAD5BC2A84258D</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FLS1HPF0-X/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FLS1HPF0-X/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-FLS1HPF0-X/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Genomic organization and expression of the 3′ end of the canine and feline enteric coronaviruses</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1992</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Regular article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Genomic organization and expression of the 3′ end of the canine and feline enteric coronaviruses</title><author xml:id="author-0000"><persName><forename type="first">H.</forename><surname>Vennema</surname></persName><note type="biography">To whom reprint requests should be addressed.</note><affiliation>To whom reprint requests should be addressed.</affiliation><affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</affiliation></author><author xml:id="author-0001"><persName><forename type="first">J.W.A.</forename><surname>Rossen</surname></persName><affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</affiliation></author><author xml:id="author-0002"><persName><forename type="first">J.</forename><surname>Wesseling</surname></persName><affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</affiliation></author><author xml:id="author-0003"><persName><forename type="first">M.C.</forename><surname>Horzinek</surname></persName><affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</affiliation></author><author xml:id="author-0004"><persName><forename type="first">P.J.M.</forename><surname>Rottier</surname></persName><affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</affiliation></author><idno type="istex">9F3B89832434E67F6645DAC582FAD5BC2A84258D</idno><idno type="ark">ark:/67375/6H6-FLS1HPF0-X</idno><idno type="DOI">10.1016/0042-6822(92)90174-N</idno><idno type="PII">0042-6822(92)90174-N</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0395-9</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992"></date><biblScope unit="volume">191</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="134">134</biblScope><biblScope unit="page" to="140">140</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1992</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The genomic organization at the 3′ end of canine coronavirus (CCV) and feline enteric coronavirus (FECV) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (FIPV) and transmissible gastroenteritis virus (TGEV) of swine. Comparison of the latter two has previously revealed an extra open reading frame (ORF) at the 3′ end of the FIPV genome, lacking in TGEV, which is currently designated ORF 6b. Both CCV and FECV possess 6b-related ORFs at the 3′ ends of their genomes. The presence of ORF 6b in three of four viruses in this antigenic cluster strongly suggests that TGEV has lost this ORF by deletion. The CCV ORF 6b is collinear with that of FIPV, but the predicted amino acid sequences are only 58% identical. The FECV ORF 6b contains a large deletion compared to that of FIPV, reducing the collinear part to 60%. The sequence homologies were highest between CCV and TGEV on the one hand and between FECV and FIPV on the other. Previously, we showed that the expression product of the FIPV ORF 6b can be detected in infected cells by immunoprecipitation (Vennema et al., 1992). In the present study we have performed similar experiments with CCV and FECV. In infected cells both viruses produced proteins related to but different from the FIPV 6b protein.</p></abstract></profileDesc><revisionDesc><change when="1992">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FLS1HPF0-X/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>9290174N</aid><ce:pii>0042-6822(92)90174-N</ce:pii><ce:doi>10.1016/0042-6822(92)90174-N</ce:doi><ce:copyright type="unknown" year="1992"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular article</ce:textfn></ce:dochead><ce:title>Genomic organization and expression of the 3′ end of the canine and feline enteric coronaviruses</ce:title><ce:author-group><ce:author><ce:given-name>H.</ce:given-name><ce:surname>Vennema</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.W.A.</ce:given-name><ce:surname>Rossen</ce:surname></ce:author><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Wesseling</ce:surname></ce:author><ce:author><ce:given-name>M.C.</ce:given-name><ce:surname>Horzinek</ce:surname></ce:author><ce:author><ce:given-name>P.J.M.</ce:given-name><ce:surname>Rottier</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom reprint requests should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="5" month="5" year="1992"></ce:date-received><ce:date-accepted day="1" month="7" year="1992"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The genomic organization at the 3′ end of canine coronavirus (CCV) and feline enteric coronavirus (FECV) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (FIPV) and transmissible gastroenteritis virus (TGEV) of swine. Comparison of the latter two has previously revealed an extra open reading frame (ORF) at the 3′ end of the FIPV genome, lacking in TGEV, which is currently designated ORF 6b. Both CCV and FECV possess 6b-related ORFs at the 3′ ends of their genomes. The presence of ORF 6b in three of four viruses in this antigenic cluster strongly suggests that TGEV has lost this ORF by deletion. The CCV ORF 6b is collinear with that of FIPV, but the predicted amino acid sequences are only 58% identical. The FECV ORF 6b contains a large deletion compared to that of FIPV, reducing the collinear part to 60%. The sequence homologies were highest between CCV and TGEV on the one hand and between FECV and FIPV on the other. Previously, we showed that the expression product of the FIPV ORF 6b can be detected in infected cells by immunoprecipitation (Vennema <ce:italic>et al.</ce:italic>, 1992). In the present study we have performed similar experiments with CCV and FECV. In infected cells both viruses produced proteins related to but different from the FIPV 6b protein.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Genomic organization and expression of the 3′ end of the canine and feline enteric coronaviruses</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Genomic organization and expression of the 3′ end of the canine and feline enteric coronaviruses</title></titleInfo><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Vennema</namePart><affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</affiliation><description>To whom reprint requests should be addressed.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.W.A.</namePart><namePart type="family">Rossen</namePart><affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Wesseling</namePart><affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.C.</namePart><namePart type="family">Horzinek</namePart><affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.J.M.</namePart><namePart type="family">Rottier</namePart><affiliation>Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, Yalelaan 1, P.O. Box 80. 165, 3508 TD Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The genomic organization at the 3′ end of canine coronavirus (CCV) and feline enteric coronavirus (FECV) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (FIPV) and transmissible gastroenteritis virus (TGEV) of swine. Comparison of the latter two has previously revealed an extra open reading frame (ORF) at the 3′ end of the FIPV genome, lacking in TGEV, which is currently designated ORF 6b. Both CCV and FECV possess 6b-related ORFs at the 3′ ends of their genomes. The presence of ORF 6b in three of four viruses in this antigenic cluster strongly suggests that TGEV has lost this ORF by deletion. The CCV ORF 6b is collinear with that of FIPV, but the predicted amino acid sequences are only 58% identical. The FECV ORF 6b contains a large deletion compared to that of FIPV, reducing the collinear part to 60%. The sequence homologies were highest between CCV and TGEV on the one hand and between FECV and FIPV on the other. Previously, we showed that the expression product of the FIPV ORF 6b can be detected in infected cells by immunoprecipitation (Vennema et al., 1992). In the present study we have performed similar experiments with CCV and FECV. In infected cells both viruses produced proteins related to but different from the FIPV 6b protein.</abstract><note type="content">Section title: Regular article</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0395-9</identifier><part><date>1992</date><detail type="volume"><number>191</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>532</end></extent><extent unit="pages"><start>134</start><end>140</end></extent></part></relatedItem><identifier type="istex">9F3B89832434E67F6645DAC582FAD5BC2A84258D</identifier><identifier type="ark">ark:/67375/6H6-FLS1HPF0-X</identifier><identifier type="DOI">10.1016/0042-6822(92)90174-N</identifier><identifier type="PII">0042-6822(92)90174-N</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FLS1HPF0-X/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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