Analysis of cis -Acting Sequences Essential for Coronavirus Defective Interfering RNA Replication
Identifieur interne : 000244 ( Istex/Corpus ); précédent : 000243; suivant : 000245Analysis of cis -Acting Sequences Essential for Coronavirus Defective Interfering RNA Replication
Auteurs : Young-Nam Kim ; Yong Seok Jeong ; Shinji MakinoSource :
- Virology [ 0042-6822 ] ; 1993.
Abstract
Abstract: Mouse hepatitis virus (MHV) defective interfering (DI) RNA was used to determine the cis-acting sequences required for MHV RNA replication. A 2.2-kb-long cDNA clone of the MHV DI RNA DIssE was used to test the effect of deletions throughout the DI RNA on replication in DI RNA-transfected, MHV-infected cells. Data from a series of deletion mutants demonstrated that about 470 nucleotides at the 5′ terminus, 460 nucleotides at the 3′ terminus, and about 135 nucleotides in an internal position approximately 0.9 kb from the 5′ end of DI RNA were necessary for DI RNA replication. These data suggested that cis-acting sequences which were necessary for MHV RNA replication required not only terminal sequences but also an internal sequence present at about 3.2 kb from the 5′ end of the genome.
Url:
DOI: 10.1006/viro.1993.1566
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A 2.2-kb-long cDNA clone of the MHV DI RNA DIssE was used to test the effect of deletions throughout the DI RNA on replication in DI RNA-transfected, MHV-infected cells. Data from a series of deletion mutants demonstrated that about 470 nucleotides at the 5′ terminus, 460 nucleotides at the 3′ terminus, and about 135 nucleotides in an internal position approximately 0.9 kb from the 5′ end of DI RNA were necessary for DI RNA replication. These data suggested that cis-acting sequences which were necessary for MHV RNA replication required not only terminal sequences but also an internal sequence present at about 3.2 kb from the 5′ end of the genome.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Young-Nam Kim</name><affiliations><json:string>Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712-1095</json:string></affiliations></json:item><json:item><name>Yong Seok Jeong</name><affiliations><json:string>Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712-1095</json:string></affiliations></json:item><json:item><name>Shinji Makino</name><affiliations><json:string>Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712-1095</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-GSLCCMJS-N</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Mouse hepatitis virus (MHV) defective interfering (DI) RNA was used to determine the cis-acting sequences required for MHV RNA replication. A 2.2-kb-long cDNA clone of the MHV DI RNA DIssE was used to test the effect of deletions throughout the DI RNA on replication in DI RNA-transfected, MHV-infected cells. Data from a series of deletion mutants demonstrated that about 470 nucleotides at the 5′ terminus, 460 nucleotides at the 3′ terminus, and about 135 nucleotides in an internal position approximately 0.9 kb from the 5′ end of DI RNA were necessary for DI RNA replication. 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A 2.2-kb-long cDNA clone of the MHV DI RNA DIssE was used to test the effect of deletions throughout the DI RNA on replication in DI RNA-transfected, MHV-infected cells. Data from a series of deletion mutants demonstrated that about 470 nucleotides at the 5′ terminus, 460 nucleotides at the 3′ terminus, and about 135 nucleotides in an internal position approximately 0.9 kb from the 5′ end of DI RNA were necessary for DI RNA replication. These data suggested that cis-acting sequences which were necessary for MHV RNA replication required not only terminal sequences but also an internal sequence present at about 3.2 kb from the 5′ end of the genome.</p></abstract></profileDesc><revisionDesc><change when="1993">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-GSLCCMJS-N/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YVIRO</jid><aid>71566</aid><ce:pii>S0042-6822(83)71566-7</ce:pii><ce:doi>10.1006/viro.1993.1566</ce:doi><ce:copyright type="full-transfer" year="1993">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Analysis of <ce:italic>cis</ce:italic>-Acting Sequences Essential for Coronavirus Defective Interfering RNA Replication</ce:title><ce:author-group><ce:author><ce:given-name>Young-Nam</ce:given-name><ce:surname>Kim</ce:surname></ce:author><ce:author><ce:given-name>Yong Seok</ce:given-name><ce:surname>Jeong</ce:surname></ce:author><ce:author><ce:given-name>Shinji</ce:given-name><ce:surname>Makino</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712-1095</ce:textfn></ce:affiliation></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Mouse hepatitis virus (MHV) defective interfering (DI) RNA was used to determine the cis-acting sequences required for MHV RNA replication. A 2.2-kb-long cDNA clone of the MHV DI RNA DIssE was used to test the effect of deletions throughout the DI RNA on replication in DI RNA-transfected, MHV-infected cells. Data from a series of deletion mutants demonstrated that about 470 nucleotides at the 5′ terminus, 460 nucleotides at the 3′ terminus, and about 135 nucleotides in an internal position approximately 0.9 kb from the 5′ end of DI RNA were necessary for DI RNA replication. These data suggested that <ce:italic>cis</ce:italic>-acting sequences which were necessary for MHV RNA replication required not only terminal sequences but also an internal sequence present at about 3.2 kb from the 5′ end of the genome.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Analysis of cis -Acting Sequences Essential for Coronavirus Defective Interfering RNA Replication</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Analysis of</title></titleInfo><name type="personal"><namePart type="given">Young-Nam</namePart><namePart type="family">Kim</namePart><affiliation>Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712-1095</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yong Seok</namePart><namePart type="family">Jeong</namePart><affiliation>Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712-1095</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shinji</namePart><namePart type="family">Makino</namePart><affiliation>Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712-1095</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Mouse hepatitis virus (MHV) defective interfering (DI) RNA was used to determine the cis-acting sequences required for MHV RNA replication. A 2.2-kb-long cDNA clone of the MHV DI RNA DIssE was used to test the effect of deletions throughout the DI RNA on replication in DI RNA-transfected, MHV-infected cells. Data from a series of deletion mutants demonstrated that about 470 nucleotides at the 5′ terminus, 460 nucleotides at the 3′ terminus, and about 135 nucleotides in an internal position approximately 0.9 kb from the 5′ end of DI RNA were necessary for DI RNA replication. These data suggested that cis-acting sequences which were necessary for MHV RNA replication required not only terminal sequences but also an internal sequence present at about 3.2 kb from the 5′ end of the genome.</abstract><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0180-8</identifier><part><date>1993</date><detail type="volume"><number>197</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>510</end></extent><extent unit="pages"><start>53</start><end>63</end></extent></part></relatedItem><identifier type="istex">7705555B30554C801200167D9F50418A1BBECFF2</identifier><identifier type="ark">ark:/67375/6H6-GSLCCMJS-N</identifier><identifier type="DOI">10.1006/viro.1993.1566</identifier><identifier type="PII">S0042-6822(83)71566-7</identifier><accessCondition type="use and reproduction" contentType="copyright">©1993 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1993</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-GSLCCMJS-N/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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