Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows
Identifieur interne : 000234 ( Istex/Corpus ); précédent : 000233; suivant : 000235Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows
Auteurs : Ignacio Lanza ; Pedro Rubio ; Máximo Fernández ; María Mu Oz ; Pedro CármenesSource :
- Preventive Veterinary Medicine [ 0167-5877 ] ; 1993.
English descriptors
- Teeft :
- Callebaut, Castilla, Coronavirus, Different herd categories, Elisa, Elisa values, Family herds, Farm categories, Farm size, Gastroenteritis, Herd size, Inhibition elisa, Lanza, Le6n, Macelisa, Management practices, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Monoclonal elisa, Other european countries, Other hand, Other parts, Pensaert, Piglet, Porcine, Positive sera, Prcv, Prcv infection, Prevalence rates, Rubio, Seropositivity, Serum samples, Significant differences, Statistical analysis, Tgev, Tgev infection, Transmissible, Transmissible gastroenteritis virus, Viral.
Abstract
Abstract: The seroprevalence of porcine respiratory coronavirus (PRCV) among breeding sows was estimated in Castilla y León, the largest region in Spain, by a cross-sectional study. Serum samples (1247) were taken from sows from 58 different herds for this purpose throughout 1988 and were tested by a monoclonal antibody-capture ELISA (MACELISA), a test which detects antibodies to both transmissible gastroenteritis virus (TGEV) and PRCV. In order to discriminate positive sera to both coronaviruses, a blocking inhibition ELISA was used for all MACELISA-positive sera. By MACELISA, 31.4% of sera tested were positive and all of them were confirmed specifically as PRCV-positive by blocking inhibition ELISA, thus ruling out the presence of TGEV in the sampled area: 64% of the farms had at least one PRCV-seropositive sow, indicating that the infection was widespread Farms were classified into three categories, according to size and management practices. No significant differences were found in the prevalence rates among the three farm types; there was, however, a highly significant correlation between increasing farm size and increasing within-farm seropositivity.
Url:
DOI: 10.1016/0167-5877(93)90034-Q
Links to Exploration step
ISTEX:ACC9632F36483C01EB95F55C4D4CFABF958B4D5FLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows</title><author><name sortKey="Lanza, Ignacio" sort="Lanza, Ignacio" uniqKey="Lanza I" first="Ignacio" last="Lanza">Ignacio Lanza</name><affiliation><mods:affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Rubio, Pedro" sort="Rubio, Pedro" uniqKey="Rubio P" first="Pedro" last="Rubio">Pedro Rubio</name><affiliation><mods:affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Fernandez, Maximo" sort="Fernandez, Maximo" uniqKey="Fernandez M" first="Máximo" last="Fernández">Máximo Fernández</name><affiliation><mods:affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Mu Oz, Maria" sort="Mu Oz, Maria" uniqKey="Mu Oz M" first="María" last="Mu Oz">María Mu Oz</name><affiliation><mods:affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Carmenes, Pedro" sort="Carmenes, Pedro" uniqKey="Carmenes P" first="Pedro" last="Cármenes">Pedro Cármenes</name><affiliation><mods:affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:ACC9632F36483C01EB95F55C4D4CFABF958B4D5F</idno><date when="1993" year="1993">1993</date><idno type="doi">10.1016/0167-5877(93)90034-Q</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-90XVBBSZ-V/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000234</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000234</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows</title><author><name sortKey="Lanza, Ignacio" sort="Lanza, Ignacio" uniqKey="Lanza I" first="Ignacio" last="Lanza">Ignacio Lanza</name><affiliation><mods:affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Rubio, Pedro" sort="Rubio, Pedro" uniqKey="Rubio P" first="Pedro" last="Rubio">Pedro Rubio</name><affiliation><mods:affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Fernandez, Maximo" sort="Fernandez, Maximo" uniqKey="Fernandez M" first="Máximo" last="Fernández">Máximo Fernández</name><affiliation><mods:affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Mu Oz, Maria" sort="Mu Oz, Maria" uniqKey="Mu Oz M" first="María" last="Mu Oz">María Mu Oz</name><affiliation><mods:affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Carmenes, Pedro" sort="Carmenes, Pedro" uniqKey="Carmenes P" first="Pedro" last="Cármenes">Pedro Cármenes</name><affiliation><mods:affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Preventive Veterinary Medicine</title><title level="j" type="abbrev">PREVET</title><idno type="ISSN">0167-5877</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">17</biblScope><biblScope unit="issue">3–4</biblScope><biblScope unit="page" from="263">263</biblScope><biblScope unit="page" to="269">269</biblScope></imprint><idno type="ISSN">0167-5877</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0167-5877</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Callebaut</term><term>Castilla</term><term>Coronavirus</term><term>Different herd categories</term><term>Elisa</term><term>Elisa values</term><term>Family herds</term><term>Farm categories</term><term>Farm size</term><term>Gastroenteritis</term><term>Herd size</term><term>Inhibition elisa</term><term>Lanza</term><term>Le6n</term><term>Macelisa</term><term>Management practices</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Monoclonal antibody</term><term>Monoclonal elisa</term><term>Other european countries</term><term>Other hand</term><term>Other parts</term><term>Pensaert</term><term>Piglet</term><term>Porcine</term><term>Positive sera</term><term>Prcv</term><term>Prcv infection</term><term>Prevalence rates</term><term>Rubio</term><term>Seropositivity</term><term>Serum samples</term><term>Significant differences</term><term>Statistical analysis</term><term>Tgev</term><term>Tgev infection</term><term>Transmissible</term><term>Transmissible gastroenteritis virus</term><term>Viral</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The seroprevalence of porcine respiratory coronavirus (PRCV) among breeding sows was estimated in Castilla y León, the largest region in Spain, by a cross-sectional study. Serum samples (1247) were taken from sows from 58 different herds for this purpose throughout 1988 and were tested by a monoclonal antibody-capture ELISA (MACELISA), a test which detects antibodies to both transmissible gastroenteritis virus (TGEV) and PRCV. In order to discriminate positive sera to both coronaviruses, a blocking inhibition ELISA was used for all MACELISA-positive sera. By MACELISA, 31.4% of sera tested were positive and all of them were confirmed specifically as PRCV-positive by blocking inhibition ELISA, thus ruling out the presence of TGEV in the sampled area: 64% of the farms had at least one PRCV-seropositive sow, indicating that the infection was widespread Farms were classified into three categories, according to size and management practices. No significant differences were found in the prevalence rates among the three farm types; there was, however, a highly significant correlation between increasing farm size and increasing within-farm seropositivity.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>prcv</json:string><json:string>elisa</json:string><json:string>tgev</json:string><json:string>coronavirus</json:string><json:string>porcine</json:string><json:string>gastroenteritis</json:string><json:string>elisa values</json:string><json:string>le6n</json:string><json:string>seropositivity</json:string><json:string>transmissible gastroenteritis virus</json:string><json:string>castilla</json:string><json:string>macelisa</json:string><json:string>monoclonal</json:string><json:string>callebaut</json:string><json:string>pensaert</json:string><json:string>management practices</json:string><json:string>rubio</json:string><json:string>viral</json:string><json:string>piglet</json:string><json:string>prcv infection</json:string><json:string>transmissible</json:string><json:string>farm size</json:string><json:string>significant differences</json:string><json:string>positive sera</json:string><json:string>tgev infection</json:string><json:string>different herd categories</json:string><json:string>farm categories</json:string><json:string>other european countries</json:string><json:string>lanza</json:string><json:string>family herds</json:string><json:string>monoclonal antibodies</json:string><json:string>monoclonal antibody</json:string><json:string>monoclonal elisa</json:string><json:string>statistical analysis</json:string><json:string>inhibition elisa</json:string><json:string>other hand</json:string><json:string>herd size</json:string><json:string>serum samples</json:string><json:string>other parts</json:string><json:string>prevalence rates</json:string></teeft></keywords><author><json:item><name>Ignacio Lanza</name><affiliations><json:string>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</json:string></affiliations></json:item><json:item><name>Pedro Rubio</name><affiliations><json:string>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</json:string></affiliations></json:item><json:item><name>Máximo Fernández</name><affiliations><json:string>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</json:string></affiliations></json:item><json:item><name>María Muńoz</name><affiliations><json:string>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</json:string></affiliations></json:item><json:item><name>Pedro Cármenes</name><affiliations><json:string>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-90XVBBSZ-V</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The seroprevalence of porcine respiratory coronavirus (PRCV) among breeding sows was estimated in Castilla y León, the largest region in Spain, by a cross-sectional study. Serum samples (1247) were taken from sows from 58 different herds for this purpose throughout 1988 and were tested by a monoclonal antibody-capture ELISA (MACELISA), a test which detects antibodies to both transmissible gastroenteritis virus (TGEV) and PRCV. In order to discriminate positive sera to both coronaviruses, a blocking inhibition ELISA was used for all MACELISA-positive sera. By MACELISA, 31.4% of sera tested were positive and all of them were confirmed specifically as PRCV-positive by blocking inhibition ELISA, thus ruling out the presence of TGEV in the sampled area: 64% of the farms had at least one PRCV-seropositive sow, indicating that the infection was widespread Farms were classified into three categories, according to size and management practices. No significant differences were found in the prevalence rates among the three farm types; there was, however, a highly significant correlation between increasing farm size and increasing within-farm seropositivity.</abstract><qualityIndicators><score>6.412</score><pdfWordCount>2348</pdfWordCount><pdfCharCount>14380</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>7</pdfPageCount><pdfPageSize>461 x 677 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>172</abstractWordCount><abstractCharCount>1174</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows</title><pii><json:string>0167-5877(93)90034-Q</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Preventive Veterinary Medicine</title><language><json:string>unknown</json:string></language><publicationDate>1993</publicationDate><issn><json:string>0167-5877</json:string></issn><pii><json:string>S0167-5877(00)X0133-X</json:string></pii><volume>17</volume><issue>3–4</issue><pages><first>263</first><last>269</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1986</json:string><json:string>1993</json:string><json:string>1247</json:string><json:string>1988</json:string><json:string>1985</json:string></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>I. Lanza</json:string><json:string>Maria Mufioz</json:string><json:string>G.F. Bay</json:string><json:string>Pedro Cdrmenes</json:string><json:string>Pedro Rubio</json:string><json:string>L. Enjuanes</json:string></persName><placeName><json:string>US</json:string><json:string>Madrid</json:string><json:string>Europe</json:string><json:string>Denmark</json:string><json:string>Murcia</json:string><json:string>Spain</json:string><json:string>Belgium</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Rubio et al. (1987)</json:string><json:string>Cubero-Pablo et al., 1990</json:string><json:string>Callebaut et al. (1988b)</json:string><json:string>L Lanza et al.</json:string><json:string>M. Martin et al.</json:string><json:string>I. Lanza et aL</json:string><json:string>Wesley et al., 1990</json:string><json:string>Martin et al., 1987</json:string><json:string>Dean et al., 1990</json:string><json:string>Bourgueil et al., 1992</json:string><json:string>L Lanza et aL</json:string><json:string>Callebaut et al., 1988a,b</json:string><json:string>Lanza et al., 1993</json:string><json:string>Henningsen et al. ( 1988 )</json:string><json:string>Laviada et al., 1988</json:string><json:string>April 1988</json:string><json:string>Lanza et al., 1992</json:string><json:string>Pensaert et al., 1986</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-90XVBBSZ-V</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - veterinary sciences</json:string></wos><scienceMetrix><json:string>1 - applied sciences</json:string><json:string>2 - agriculture, fisheries & forestry</json:string><json:string>3 - veterinary sciences</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Agricultural and Biological Sciences</json:string><json:string>3 - Animal Science and Zoology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Veterinary</json:string><json:string>3 - Food Animals</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string><json:string>4 - sante publique. hygiene-medecine du travail</json:string></inist></categories><publicationDate>1993</publicationDate><copyrightDate>1993</copyrightDate><doi><json:string>10.1016/0167-5877(93)90034-Q</json:string></doi><id>ACC9632F36483C01EB95F55C4D4CFABF958B4D5F</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-90XVBBSZ-V/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-90XVBBSZ-V/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-90XVBBSZ-V/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1993</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows</title><author xml:id="author-0000"><persName><forename type="first">Ignacio</forename><surname>Lanza</surname></persName><note type="correspondence"><p>Corresponding author.</p></note><affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Pedro</forename><surname>Rubio</surname></persName><affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Máximo</forename><surname>Fernández</surname></persName><affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</affiliation></author><author xml:id="author-0003"><persName><forename type="first">María</forename><surname>Muńoz</surname></persName><affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Pedro</forename><surname>Cármenes</surname></persName><affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</affiliation></author><idno type="istex">ACC9632F36483C01EB95F55C4D4CFABF958B4D5F</idno><idno type="ark">ark:/67375/6H6-90XVBBSZ-V</idno><idno type="DOI">10.1016/0167-5877(93)90034-Q</idno><idno type="PII">0167-5877(93)90034-Q</idno></analytic><monogr><title level="j">Preventive Veterinary Medicine</title><title level="j" type="abbrev">PREVET</title><idno type="pISSN">0167-5877</idno><idno type="PII">S0167-5877(00)X0133-X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993"></date><biblScope unit="volume">17</biblScope><biblScope unit="issue">3–4</biblScope><biblScope unit="page" from="263">263</biblScope><biblScope unit="page" to="269">269</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1993</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The seroprevalence of porcine respiratory coronavirus (PRCV) among breeding sows was estimated in Castilla y León, the largest region in Spain, by a cross-sectional study. Serum samples (1247) were taken from sows from 58 different herds for this purpose throughout 1988 and were tested by a monoclonal antibody-capture ELISA (MACELISA), a test which detects antibodies to both transmissible gastroenteritis virus (TGEV) and PRCV. In order to discriminate positive sera to both coronaviruses, a blocking inhibition ELISA was used for all MACELISA-positive sera. By MACELISA, 31.4% of sera tested were positive and all of them were confirmed specifically as PRCV-positive by blocking inhibition ELISA, thus ruling out the presence of TGEV in the sampled area: 64% of the farms had at least one PRCV-seropositive sow, indicating that the infection was widespread Farms were classified into three categories, according to size and management practices. No significant differences were found in the prevalence rates among the three farm types; there was, however, a highly significant correlation between increasing farm size and increasing within-farm seropositivity.</p></abstract></profileDesc><revisionDesc><change when="1993">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-90XVBBSZ-V/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>PREVET</jid><aid>9390034Q</aid><ce:pii>0167-5877(93)90034-Q</ce:pii><ce:doi>10.1016/0167-5877(93)90034-Q</ce:doi><ce:copyright type="unknown" year="1993"></ce:copyright></item-info><head><ce:title>Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows</ce:title><ce:author-group><ce:author><ce:given-name>Ignacio</ce:given-name><ce:surname>Lanza</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Pedro</ce:given-name><ce:surname>Rubio</ce:surname></ce:author><ce:author><ce:given-name>Máximo</ce:given-name><ce:surname>Fernández</ce:surname></ce:author><ce:author><ce:given-name>María</ce:given-name><ce:surname>Muńoz</ce:surname></ce:author><ce:author><ce:given-name>Pedro</ce:given-name><ce:surname>Cármenes</ce:surname></ce:author><ce:affiliation><ce:textfn>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Corresponding author.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="6" month="4" year="1993"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The seroprevalence of porcine respiratory coronavirus (PRCV) among breeding sows was estimated in Castilla y León, the largest region in Spain, by a cross-sectional study. Serum samples (1247) were taken from sows from 58 different herds for this purpose throughout 1988 and were tested by a monoclonal antibody-capture ELISA (MACELISA), a test which detects antibodies to both transmissible gastroenteritis virus (TGEV) and PRCV. In order to discriminate positive sera to both coronaviruses, a blocking inhibition ELISA was used for all MACELISA-positive sera. By MACELISA, 31.4% of sera tested were positive and all of them were confirmed specifically as PRCV-positive by blocking inhibition ELISA, thus ruling out the presence of TGEV in the sampled area: 64% of the farms had at least one PRCV-seropositive sow, indicating that the infection was widespread Farms were classified into three categories, according to size and management practices. No significant differences were found in the prevalence rates among the three farm types; there was, however, a highly significant correlation between increasing farm size and increasing within-farm seropositivity.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows</title></titleInfo><name type="personal"><namePart type="given">Ignacio</namePart><namePart type="family">Lanza</namePart><affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</affiliation><description>Corresponding author.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pedro</namePart><namePart type="family">Rubio</namePart><affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Máximo</namePart><namePart type="family">Fernández</namePart><affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">María</namePart><namePart type="family">Muńoz</namePart><affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pedro</namePart><namePart type="family">Cármenes</namePart><affiliation>Departamento de Sanidad Animal (Enfermedades infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Campus de Vegazana, E-24071-León, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The seroprevalence of porcine respiratory coronavirus (PRCV) among breeding sows was estimated in Castilla y León, the largest region in Spain, by a cross-sectional study. Serum samples (1247) were taken from sows from 58 different herds for this purpose throughout 1988 and were tested by a monoclonal antibody-capture ELISA (MACELISA), a test which detects antibodies to both transmissible gastroenteritis virus (TGEV) and PRCV. In order to discriminate positive sera to both coronaviruses, a blocking inhibition ELISA was used for all MACELISA-positive sera. By MACELISA, 31.4% of sera tested were positive and all of them were confirmed specifically as PRCV-positive by blocking inhibition ELISA, thus ruling out the presence of TGEV in the sampled area: 64% of the farms had at least one PRCV-seropositive sow, indicating that the infection was widespread Farms were classified into three categories, according to size and management practices. No significant differences were found in the prevalence rates among the three farm types; there was, however, a highly significant correlation between increasing farm size and increasing within-farm seropositivity.</abstract><relatedItem type="host"><titleInfo><title>Preventive Veterinary Medicine</title></titleInfo><titleInfo type="abbreviated"><title>PREVET</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued></originInfo><identifier type="ISSN">0167-5877</identifier><identifier type="PII">S0167-5877(00)X0133-X</identifier><part><date>1993</date><detail type="volume"><number>17</number><caption>vol.</caption></detail><detail type="issue"><number>3–4</number><caption>no.</caption></detail><extent unit="issue-pages"><start>137</start><end>294</end></extent><extent unit="pages"><start>263</start><end>269</end></extent></part></relatedItem><identifier type="istex">ACC9632F36483C01EB95F55C4D4CFABF958B4D5F</identifier><identifier type="ark">ark:/67375/6H6-90XVBBSZ-V</identifier><identifier type="DOI">10.1016/0167-5877(93)90034-Q</identifier><identifier type="PII">0167-5877(93)90034-Q</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-90XVBBSZ-V/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000234 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000234 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:ACC9632F36483C01EB95F55C4D4CFABF958B4D5F
|texte= Seroprevalence of porcine respiratory coronavirus infection in Spanish breeding sows
}}
| This area was generated with Dilib version V0.6.33. |  |