Putative probiotic Lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens
Identifieur interne : 000143 ( Istex/Corpus ); précédent : 000142; suivant : 000144Putative probiotic Lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens
Auteurs : Rejish Kumar V. J ; Byeong Joo Seo ; Mi Ran Mun ; Chul-Joong Kim ; Insun Lee ; Hongik Kim ; Yong-Ha ParkSource :
- Tropical Animal Health and Production [ 0049-4747 ] ; 2010-12-01.
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Abstract
Abstract: A total of 310 bacterial strains isolated from the porcine gastrointestinal tract were tested for their activity against transmissible gastroenteritis (TGE) coronavirus and other enteric pathogens. Based on activity, the strains Probio-38 and Probio-37 were selected as potential probiotics and identified as Lactobacillus plantarum Probio-38 and Lactobacillus salivarius Probio-37 respectively by 16S rRNA gene sequencing. Supernatants of these strains inhibited TGE coronavirus in vitro in ST cells, without any cytopathic effect even after 72 h of incubation. Both the strains exhibited high survival in synthetic gastric juice. The strains were resistant to 5% porcine bile and exhibited antimicrobial activity against all the 13 enteric bacterial pathogens tested. These strains also exhibited resistance to most of the antibiotics analyzed. The inhibition of transmissible gastroenteritis coronavirus and enteric bacterial pathogens as well as the bile tolerance, high survival in gastric juice, and the antibiotic resistance indicate that the two isolated bacterial strains are ideal probiotic candidates for animal application after proper in vivo experiments.
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DOI: 10.1007/s11250-010-9648-5
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J">Rejish Kumar V. J</name><affiliation><mods:affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</mods:affiliation></affiliation></author><author><name sortKey="Seo, Byeong Joo" sort="Seo, Byeong Joo" uniqKey="Seo B" first="Byeong Joo" last="Seo">Byeong Joo Seo</name><affiliation><mods:affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</mods:affiliation></affiliation></author><author><name sortKey="Mun, Mi Ran" sort="Mun, Mi Ran" uniqKey="Mun M" first="Mi Ran" last="Mun">Mi Ran Mun</name><affiliation><mods:affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</mods:affiliation></affiliation></author><author><name sortKey="Kim, Chul Joong" sort="Kim, Chul Joong" uniqKey="Kim C" first="Chul-Joong" last="Kim">Chul-Joong Kim</name><affiliation><mods:affiliation>Collage of Veterinary Medicine, Chungnam National University, 305-764, Daejeon, South Korea</mods:affiliation></affiliation></author><author><name sortKey="Lee, Insun" sort="Lee, Insun" uniqKey="Lee I" first="Insun" last="Lee">Insun Lee</name><affiliation><mods:affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</mods:affiliation></affiliation></author><author><name sortKey="Kim, Hongik" sort="Kim, Hongik" uniqKey="Kim H" first="Hongik" last="Kim">Hongik Kim</name><affiliation><mods:affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</mods:affiliation></affiliation></author><author><name sortKey="Park, Yong Ha" sort="Park, Yong Ha" uniqKey="Park Y" first="Yong-Ha" last="Park">Yong-Ha Park</name><affiliation><mods:affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: peter@ynu.ac.kr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Tropical Animal Health and Production</title><title level="j" type="abbrev">Trop Anim Health Prod</title><idno type="ISSN">0049-4747</idno><idno type="eISSN">1573-7438</idno><imprint><publisher>Springer Netherlands</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="2010-12-01">2010-12-01</date><biblScope unit="volume">42</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="1855">1855</biblScope><biblScope unit="page" to="1860">1860</biblScope></imprint><idno type="ISSN">0049-4747</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0049-4747</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibiotic sensitivity</term><term>Enteric pathogens</term><term>Lactobacillus plantarum</term><term>Lactobacillus salivarius</term><term>Transmissible gastroenteritis coronavirus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A total of 310 bacterial strains isolated from the porcine gastrointestinal tract were tested for their activity against transmissible gastroenteritis (TGE) coronavirus and other enteric pathogens. Based on activity, the strains Probio-38 and Probio-37 were selected as potential probiotics and identified as Lactobacillus plantarum Probio-38 and Lactobacillus salivarius Probio-37 respectively by 16S rRNA gene sequencing. Supernatants of these strains inhibited TGE coronavirus in vitro in ST cells, without any cytopathic effect even after 72 h of incubation. Both the strains exhibited high survival in synthetic gastric juice. The strains were resistant to 5% porcine bile and exhibited antimicrobial activity against all the 13 enteric bacterial pathogens tested. These strains also exhibited resistance to most of the antibiotics analyzed. The inhibition of transmissible gastroenteritis coronavirus and enteric bacterial pathogens as well as the bile tolerance, high survival in gastric juice, and the antibiotic resistance indicate that the two isolated bacterial strains are ideal probiotic candidates for animal application after proper in vivo experiments.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Rejish Kumar V. J</name><affiliations><json:string>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</json:string></affiliations></json:item><json:item><name>Byeong Joo Seo</name><affiliations><json:string>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</json:string></affiliations></json:item><json:item><name>Mi Ran Mun</name><affiliations><json:string>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</json:string></affiliations></json:item><json:item><name>Chul-Joong Kim</name><affiliations><json:string>Collage of Veterinary Medicine, Chungnam National University, 305-764, Daejeon, South Korea</json:string></affiliations></json:item><json:item><name>Insun Lee</name><affiliations><json:string>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</json:string></affiliations></json:item><json:item><name>Hongik Kim</name><affiliations><json:string>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</json:string></affiliations></json:item><json:item><name>Yong-Ha Park</name><affiliations><json:string>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</json:string><json:string>E-mail: peter@ynu.ac.kr</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Transmissible gastroenteritis coronavirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Lactobacillus plantarum</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Lactobacillus salivarius</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Enteric pathogens</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Antibiotic sensitivity</value></json:item></subject><articleId><json:string>9648</json:string><json:string>s11250-010-9648-5</json:string></articleId><arkIstex>ark:/67375/VQC-574LJGMZ-X</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: A total of 310 bacterial strains isolated from the porcine gastrointestinal tract were tested for their activity against transmissible gastroenteritis (TGE) coronavirus and other enteric pathogens. Based on activity, the strains Probio-38 and Probio-37 were selected as potential probiotics and identified as Lactobacillus plantarum Probio-38 and Lactobacillus salivarius Probio-37 respectively by 16S rRNA gene sequencing. Supernatants of these strains inhibited TGE coronavirus in vitro in ST cells, without any cytopathic effect even after 72 h of incubation. Both the strains exhibited high survival in synthetic gastric juice. The strains were resistant to 5% porcine bile and exhibited antimicrobial activity against all the 13 enteric bacterial pathogens tested. These strains also exhibited resistance to most of the antibiotics analyzed. The inhibition of transmissible gastroenteritis coronavirus and enteric bacterial pathogens as well as the bile tolerance, high survival in gastric juice, and the antibiotic resistance indicate that the two isolated bacterial strains are ideal probiotic candidates for animal application after proper in vivo experiments.</abstract><qualityIndicators><score>7.198</score><pdfWordCount>3254</pdfWordCount><pdfCharCount>21259</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>6</pdfPageCount><pdfPageSize>595.276 x 790.866 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>162</abstractWordCount><abstractCharCount>1178</abstractCharCount><keywordCount>5</keywordCount></qualityIndicators><title>Putative probiotic Lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens</title><genre><json:string>research-article</json:string></genre><host><title>Tropical Animal Health and Production</title><language><json:string>unknown</json:string></language><publicationDate>2010</publicationDate><copyrightDate>2010</copyrightDate><issn><json:string>0049-4747</json:string></issn><eissn><json:string>1573-7438</json:string></eissn><journalId><json:string>11250</json:string></journalId><volume>42</volume><issue>8</issue><pages><first>1855</first><last>1860</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Veterinary Medicine</value></json:item><json:item><value>Zoology</value></json:item></subject></host><ark><json:string>ark:/67375/VQC-574LJGMZ-X</json:string></ark><publicationDate>2010</publicationDate><copyrightDate>2010</copyrightDate><doi><json:string>10.1007/s11250-010-9648-5</json:string></doi><id>DCAE0259EE9EC85FE80BCAB1FE2BA93099B5D104</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-574LJGMZ-X/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-574LJGMZ-X/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/VQC-574LJGMZ-X/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Putative probiotic Lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">Springer Netherlands</publisher><pubPlace>Dordrecht</pubPlace><availability><licence><p>Springer Science+Business Media B.V., 2010</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>2010</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note>Original Research</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Putative probiotic Lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens</title><author xml:id="author-0000"><persName><forename type="first">Rejish</forename><surname>V. J</surname></persName><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Byeong</forename><surname>Seo</surname></persName><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Mi</forename><surname>Mun</surname></persName><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Chul-Joong</forename><surname>Kim</surname></persName><affiliation>Collage of Veterinary Medicine, Chungnam National University, 305-764, Daejeon, South Korea</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Insun</forename><surname>Lee</surname></persName><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Hongik</forename><surname>Kim</surname></persName><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation></author><author xml:id="author-0006" corresp="yes"><persName><forename type="first">Yong-Ha</forename><surname>Park</surname></persName><email>peter@ynu.ac.kr</email><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation></author><idno type="istex">DCAE0259EE9EC85FE80BCAB1FE2BA93099B5D104</idno><idno type="ark">ark:/67375/VQC-574LJGMZ-X</idno><idno type="DOI">10.1007/s11250-010-9648-5</idno><idno type="article-id">9648</idno><idno type="article-id">s11250-010-9648-5</idno></analytic><monogr><title level="j">Tropical Animal Health and Production</title><title level="j" type="abbrev">Trop Anim Health Prod</title><idno type="pISSN">0049-4747</idno><idno type="eISSN">1573-7438</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">39</idno><idno type="volume-issue-count">8</idno><imprint><publisher>Springer Netherlands</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="2010-12-01"></date><biblScope unit="volume">42</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="1855">1855</biblScope><biblScope unit="page" to="1860">1860</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: A total of 310 bacterial strains isolated from the porcine gastrointestinal tract were tested for their activity against transmissible gastroenteritis (TGE) coronavirus and other enteric pathogens. Based on activity, the strains Probio-38 and Probio-37 were selected as potential probiotics and identified as Lactobacillus plantarum Probio-38 and Lactobacillus salivarius Probio-37 respectively by 16S rRNA gene sequencing. Supernatants of these strains inhibited TGE coronavirus in vitro in ST cells, without any cytopathic effect even after 72 h of incubation. Both the strains exhibited high survival in synthetic gastric juice. The strains were resistant to 5% porcine bile and exhibited antimicrobial activity against all the 13 enteric bacterial pathogens tested. These strains also exhibited resistance to most of the antibiotics analyzed. The inhibition of transmissible gastroenteritis coronavirus and enteric bacterial pathogens as well as the bile tolerance, high survival in gastric juice, and the antibiotic resistance indicate that the two isolated bacterial strains are ideal probiotic candidates for animal application after proper in vivo experiments.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Transmissible gastroenteritis coronavirus</term></item><item><term>Lactobacillus plantarum</term></item><item><term>Lactobacillus salivarius</term></item><item><term>Enteric pathogens</term></item><item><term>Antibiotic sensitivity</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Veterinary Medicine</term></item><item><term>Zoology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-12-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-574LJGMZ-X/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer Netherlands</PublisherName><PublisherLocation>Dordrecht</PublisherLocation></PublisherInfo><Journal OutputMedium="All"><JournalInfo JournalProductType="NonStandardArchiveJournal" NumberingStyle="Unnumbered"><JournalID>11250</JournalID><JournalPrintISSN>0049-4747</JournalPrintISSN><JournalElectronicISSN>1573-7438</JournalElectronicISSN><JournalTitle>Tropical Animal Health and Production</JournalTitle><JournalAbbreviatedTitle>Trop Anim Health Prod</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Veterinary Medicine</JournalSubject><JournalSubject Type="Secondary">Zoology </JournalSubject></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>42</VolumeIDStart><VolumeIDEnd>42</VolumeIDEnd><VolumeIssueCount>8</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular" OutputMedium="All"><IssueInfo IssueType="Regular" TocLevels="0"><IssueIDStart>8</IssueIDStart><IssueIDEnd>8</IssueIDEnd><IssueArticleCount>39</IssueArticleCount><IssueHistory><OnlineDate><Year>2010</Year><Month>10</Month><Day>27</Day></OnlineDate><PrintDate><Year>2010</Year><Month>10</Month><Day>26</Day></PrintDate><CoverDate><Year>2010</Year><Month>12</Month></CoverDate><PricelistYear>2010</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Springer Science+Business Media B.V.</CopyrightHolderName><CopyrightYear>2010</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s11250-010-9648-5" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>9648</ArticleID><ArticleDOI>10.1007/s11250-010-9648-5</ArticleDOI><ArticleSequenceNumber>39</ArticleSequenceNumber><ArticleTitle Language="En">Putative probiotic <Emphasis Type="Italic">Lactobacillus</Emphasis> spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens</ArticleTitle><ArticleCategory>Original Research</ArticleCategory><ArticleFirstPage>1855</ArticleFirstPage><ArticleLastPage>1860</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2010</Year><Month>6</Month><Day>28</Day></RegistrationDate><Accepted><Year>2010</Year><Month>6</Month><Day>27</Day></Accepted><OnlineDate><Year>2010</Year><Month>7</Month><Day>10</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Science+Business Media B.V.</CopyrightHolderName><CopyrightYear>2010</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Rejish</GivenName><GivenName>Kumar</GivenName><FamilyName>V. J</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Byeong</GivenName><GivenName>Joo</GivenName><FamilyName>Seo</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Mi</GivenName><GivenName>Ran</GivenName><FamilyName>Mun</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Chul-Joong</GivenName><FamilyName>Kim</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Insun</GivenName><FamilyName>Lee</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Hongik</GivenName><FamilyName>Kim</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yong-Ha</GivenName><FamilyName>Park</FamilyName></AuthorName><Contact><Phone>+82-53-8103055</Phone><Fax>+82-53-8134620</Fax><Email>peter@ynu.ac.kr</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Department of Applied Microbiology and Biotechnology</OrgDivision><OrgName>Yeungnam University</OrgName><OrgAddress><Street>Kyongsan</Street><City>Kyeongbuk</City><Postcode>712-749</Postcode><Country Code="KR">South Korea</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Collage of Veterinary Medicine</OrgDivision><OrgName>Chungnam National University</OrgName><OrgAddress><City>Daejeon</City><Postcode>305-764</Postcode><Country Code="KR">South Korea</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>A total of 310 bacterial strains isolated from the porcine gastrointestinal tract were tested for their activity against transmissible gastroenteritis (TGE) coronavirus and other enteric pathogens. Based on activity, the strains Probio-38 and Probio-37 were selected as potential probiotics and identified as <Emphasis Type="Italic">Lactobacillus plantarum</Emphasis> Probio-38 and <Emphasis Type="Italic">Lactobacillus salivarius</Emphasis> Probio-37 respectively by 16S rRNA gene sequencing. Supernatants of these strains inhibited TGE coronavirus in vitro in ST cells, without any cytopathic effect even after 72 h of incubation. Both the strains exhibited high survival in synthetic gastric juice. The strains were resistant to 5% porcine bile and exhibited antimicrobial activity against all the 13 enteric bacterial pathogens tested. These strains also exhibited resistance to most of the antibiotics analyzed. The inhibition of transmissible gastroenteritis coronavirus and enteric bacterial pathogens as well as the bile tolerance, high survival in gastric juice, and the antibiotic resistance indicate that the two isolated bacterial strains are ideal probiotic candidates for animal application after proper in vivo experiments.</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>Transmissible gastroenteritis coronavirus</Keyword><Keyword><Emphasis Type="Italic">Lactobacillus plantarum</Emphasis></Keyword><Keyword><Emphasis Type="Italic">Lactobacillus salivarius</Emphasis></Keyword><Keyword>Enteric pathogens</Keyword><Keyword>Antibiotic sensitivity</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Putative probiotic Lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Putative probiotic Lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens</title></titleInfo><name type="personal"><namePart type="given">Rejish</namePart><namePart type="given">Kumar</namePart><namePart type="family">V. J</namePart><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Byeong</namePart><namePart type="given">Joo</namePart><namePart type="family">Seo</namePart><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mi</namePart><namePart type="given">Ran</namePart><namePart type="family">Mun</namePart><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Chul-Joong</namePart><namePart type="family">Kim</namePart><affiliation>Collage of Veterinary Medicine, Chungnam National University, 305-764, Daejeon, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Insun</namePart><namePart type="family">Lee</namePart><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hongik</namePart><namePart type="family">Kim</namePart><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Yong-Ha</namePart><namePart type="family">Park</namePart><affiliation>Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, 712-749, Kyeongbuk, South Korea</affiliation><affiliation>E-mail: peter@ynu.ac.kr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Netherlands</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateIssued encoding="w3cdtf">2010-12-01</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: A total of 310 bacterial strains isolated from the porcine gastrointestinal tract were tested for their activity against transmissible gastroenteritis (TGE) coronavirus and other enteric pathogens. Based on activity, the strains Probio-38 and Probio-37 were selected as potential probiotics and identified as Lactobacillus plantarum Probio-38 and Lactobacillus salivarius Probio-37 respectively by 16S rRNA gene sequencing. Supernatants of these strains inhibited TGE coronavirus in vitro in ST cells, without any cytopathic effect even after 72 h of incubation. Both the strains exhibited high survival in synthetic gastric juice. The strains were resistant to 5% porcine bile and exhibited antimicrobial activity against all the 13 enteric bacterial pathogens tested. These strains also exhibited resistance to most of the antibiotics analyzed. The inhibition of transmissible gastroenteritis coronavirus and enteric bacterial pathogens as well as the bile tolerance, high survival in gastric juice, and the antibiotic resistance indicate that the two isolated bacterial strains are ideal probiotic candidates for animal application after proper in vivo experiments.</abstract><note>Original Research</note><subject lang="en"><genre>Keywords</genre><topic>Transmissible gastroenteritis coronavirus</topic><topic>Lactobacillus plantarum</topic><topic>Lactobacillus salivarius</topic><topic>Enteric pathogens</topic><topic>Antibiotic sensitivity</topic></subject><relatedItem type="host"><titleInfo><title>Tropical Animal Health and Production</title></titleInfo><titleInfo type="abbreviated"><title>Trop Anim Health Prod</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2010-10-27</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Veterinary Medicine</topic><topic>Zoology</topic></subject><identifier type="ISSN">0049-4747</identifier><identifier type="eISSN">1573-7438</identifier><identifier type="JournalID">11250</identifier><identifier type="IssueArticleCount">39</identifier><identifier type="VolumeIssueCount">8</identifier><part><date>2010</date><detail type="volume"><number>42</number><caption>vol.</caption></detail><detail type="issue"><number>8</number><caption>no.</caption></detail><extent unit="pages"><start>1855</start><end>1860</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media B.V., 2010</recordOrigin></recordInfo></relatedItem><identifier type="istex">DCAE0259EE9EC85FE80BCAB1FE2BA93099B5D104</identifier><identifier type="ark">ark:/67375/VQC-574LJGMZ-X</identifier><identifier type="DOI">10.1007/s11250-010-9648-5</identifier><identifier type="ArticleID">9648</identifier><identifier type="ArticleID">s11250-010-9648-5</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media B.V., 2010</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer Science+Business Media B.V., 2010</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-574LJGMZ-X/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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