A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona Virus
Identifieur interne : 000117 ( Istex/Corpus ); précédent : 000116; suivant : 000118A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona Virus
Auteurs : Il Young Jung ; Jae Bem You ; Bo Ram Choi ; Ji Su Kim ; Hyun Kyung Lee ; Bora Jang ; Han Saem Jeong ; Kyuri Lee ; Sung Gap Im ; Hyukjin LeeSource :
- Advanced Healthcare Materials [ 2192-2640 ] ; 2016-09.
Abstract
Trail polymerization enables a significant enhancement of the DhITACT system. DhITACT‐Trail (DNA hydrogel formation by isothermal amplification of complementary targets trail polymerization) offers a robust diagnosis of target RNA strands in pseudo‐serum specimen. This system requires minimum liquid handling as compared to conventional analysis. In addition, a definitive diagnostic result can be achieved within 30 min by an optical detection.
Url:
DOI: 10.1002/adhm.201600334
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Seoul, Republic of Korea</mods:affiliation></affiliation></author><author><name sortKey="You, Jae Bem" sort="You, Jae Bem" uniqKey="You J" first="Jae Bem" last="You">Jae Bem You</name><affiliation><mods:affiliation>Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 34141, Daejeon, Republic of Korea</mods:affiliation></affiliation></author><author><name sortKey="Choi, Bo Ram" sort="Choi, Bo Ram" uniqKey="Choi B" first="Bo Ram" last="Choi">Bo Ram Choi</name><affiliation><mods:affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</mods:affiliation></affiliation></author><author><name sortKey="Kim, Ji Su" sort="Kim, Ji Su" uniqKey="Kim J" first="Ji Su" last="Kim">Ji Su Kim</name><affiliation><mods:affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</mods:affiliation></affiliation></author><author><name sortKey="Lee, Hyun Kyung" sort="Lee, Hyun Kyung" uniqKey="Lee H" first="Hyun Kyung" last="Lee">Hyun Kyung Lee</name><affiliation><mods:affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</mods:affiliation></affiliation></author><author><name sortKey="Jang, Bora" sort="Jang, Bora" uniqKey="Jang B" first="Bora" last="Jang">Bora Jang</name><affiliation><mods:affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</mods:affiliation></affiliation></author><author><name sortKey="Jeong, Han Saem" sort="Jeong, Han Saem" uniqKey="Jeong H" first="Han Saem" last="Jeong">Han Saem Jeong</name><affiliation><mods:affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</mods:affiliation></affiliation></author><author><name sortKey="Lee, Kyuri" sort="Lee, Kyuri" uniqKey="Lee K" first="Kyuri" last="Lee">Kyuri Lee</name><affiliation><mods:affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</mods:affiliation></affiliation></author><author><name sortKey="Im, Sung Gap" sort="Im, Sung Gap" uniqKey="Im S" first="Sung Gap" last="Im">Sung Gap Im</name><affiliation><mods:affiliation>Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 34141, Daejeon, Republic of Korea</mods:affiliation></affiliation><affiliation><mods:affiliation>KAIST Institute for Nano Century, Korea Advanced Institute of Science and Technology (KAIST), 34141, Daejeon, Republic of Korea</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: sgim@kaist.ac.kr</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: sgim@kaist.ac.kr</mods:affiliation></affiliation></author><author><name sortKey="Lee, Hyukjin" sort="Lee, Hyukjin" uniqKey="Lee H" first="Hyukjin" last="Lee">Hyukjin Lee</name><affiliation><mods:affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: hyukjin@ewha.ac.kr</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: sgim@kaist.ac.kr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Advanced Healthcare Materials</title><title level="j" type="alt">ADVANCED HEALTHCARE MATERIALS</title><idno type="ISSN">2192-2640</idno><idno type="eISSN">2192-2659</idno><imprint><biblScope unit="vol">5</biblScope><biblScope unit="issue">17</biblScope><biblScope unit="page" from="2168">2168</biblScope><biblScope unit="page" to="2173">2173</biblScope><biblScope 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DhITACT‐Trail (DNA hydrogel formation by isothermal amplification of complementary targets trail polymerization) offers a robust diagnosis of target RNA strands in pseudo‐serum specimen. This system requires minimum liquid handling as compared to conventional analysis. 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DhITACT‐Trail (DNA hydrogel formation by isothermal amplification of complementary targets trail polymerization) offers a robust diagnosis of target RNA strands in pseudo‐serum specimen. This system requires minimum liquid handling as compared to conventional analysis. 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(KAIST)</orgName><address><settlement>Daejeon</settlement><postCode>34141</postCode><country key="KR" xml:lang="en">KOREA, REPUBLIC OF</country></address></affiliation><affiliation><orgName type="division">KAIST Institute for Nano Century</orgName><orgName type="institution">Korea Advanced Institute of Science and Technology (KAIST)</orgName><address><settlement>Daejeon</settlement><postCode>34141</postCode><country key="KR" xml:lang="en">KOREA, REPUBLIC OF</country></address></affiliation></author><author xml:id="author-0009" role="corresp"><persName><forename type="first">Hyukjin</forename><surname>Lee</surname></persName><email>hyukjin@ewha.ac.kr</email><affiliation><orgName type="division">College of Pharmacy</orgName><orgName type="division">Graduate School of Pharmaceutical Sciences</orgName><orgName type="institution">Ewha Womans University</orgName><address><settlement>Seoul</settlement><postCode>120‐750</postCode><country key="KR" xml:lang="en">KOREA, REPUBLIC 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KGaA, Weinheim</copyright><eventGroup><event type="manuscriptReceived" date="2016-03-31"></event><event type="manuscriptRevised" date="2006-04-29"></event><event type="xmlCreated" agent="Aptara" date="2016-06-21"></event><event type="firstOnline" date="2016-06-22"></event><event type="publishedOnlineEarlyUnpaginated" date="2016-06-22"></event><event type="publishedOnlineFinalForm" date="2016-09-07"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:5.0.6 mode:FullText" date="2017-02-09"></event></eventGroup><numberingGroup><numbering type="pageFirst">2168</numbering><numbering type="pageLast">2173</numbering></numberingGroup><correspondenceTo>E‐mail: <email>sgim@kaist.ac.kr</email>, <email>hyukjin@ewha.ac.kr</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ADHM.ADHM201600334.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="0"></count><count type="wordTotal" number="4408"></count></countGroup><titleGroup><title type="main">A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona Virus</title></titleGroup><creators><creator xml:id="adhm201600334-cr-0001" creatorRole="author" affiliationRef="#adhm201600334-aff-0001"><personName><givenNames>Il Young</givenNames><familyName>Jung</familyName></personName></creator><creator xml:id="adhm201600334-cr-0002" creatorRole="author" affiliationRef="#adhm201600334-aff-0002"><personName><givenNames>Jae Bem</givenNames><familyName>You</familyName></personName></creator><creator xml:id="adhm201600334-cr-0003" creatorRole="author" affiliationRef="#adhm201600334-aff-0001"><personName><givenNames>Bo Ram</givenNames><familyName>Choi</familyName></personName></creator><creator xml:id="adhm201600334-cr-0004" creatorRole="author" affiliationRef="#adhm201600334-aff-0001"><personName><givenNames>Ji Su</givenNames><familyName>Kim</familyName></personName></creator><creator xml:id="adhm201600334-cr-0005" creatorRole="author" affiliationRef="#adhm201600334-aff-0001"><personName><givenNames>Hyun Kyung</givenNames><familyName>Lee</familyName></personName></creator><creator xml:id="adhm201600334-cr-0006" creatorRole="author" affiliationRef="#adhm201600334-aff-0001"><personName><givenNames>Bora</givenNames><familyName>Jang</familyName></personName></creator><creator xml:id="adhm201600334-cr-0007" creatorRole="author" affiliationRef="#adhm201600334-aff-0001"><personName><givenNames>Han Saem</givenNames><familyName>Jeong</familyName></personName></creator><creator xml:id="adhm201600334-cr-0008" creatorRole="author" affiliationRef="#adhm201600334-aff-0001"><personName><givenNames>Kyuri</givenNames><familyName>Lee</familyName></personName></creator><creator xml:id="adhm201600334-cr-0009" creatorRole="author" affiliationRef="#adhm201600334-aff-0002 #adhm201600334-aff-0003" corresponding="yes"><personName><givenNames>Sung Gap</givenNames><familyName>Im</familyName></personName><contactDetails><email>sgim@kaist.ac.kr</email></contactDetails></creator><creator xml:id="adhm201600334-cr-0010" creatorRole="author" affiliationRef="#adhm201600334-aff-0001" corresponding="yes"><personName><givenNames>Hyukjin</givenNames><familyName>Lee</familyName></personName><contactDetails><email>hyukjin@ewha.ac.kr</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="adhm201600334-aff-0001" countryCode="KR"><orgDiv>College of Pharmacy</orgDiv><orgDiv>Graduate School of Pharmaceutical Sciences</orgDiv><orgName>Ewha Womans University</orgName><address><city>Seoul</city><postCode>120‐750</postCode><country>Republic of Korea</country></address></affiliation><affiliation xml:id="adhm201600334-aff-0002" countryCode="KR"><orgDiv>Department of Chemical and Biomolecular Engineering</orgDiv><orgName>Korea Advanced Institute of Science and Technology (KAIST)</orgName><address><city>Daejeon</city><postCode>34141</postCode><country>Republic of Korea</country></address></affiliation><affiliation xml:id="adhm201600334-aff-0003" countryCode="KR"><orgDiv>KAIST Institute for Nano Century</orgDiv><orgName>Korea Advanced Institute of Science and Technology (KAIST)</orgName><address><city>Daejeon</city><postCode>34141</postCode><country>Republic of Korea</country></address></affiliation></affiliationGroup><supportingInformation><p>As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re‐organized for online delivery, but are not copy‐edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.</p><supportingInfoItem><mediaResource alt="supplementary_material" rendition="webOriginal" href="urn-x:wiley:21922640:media:adhm201600334:adhm201600334-sup-0001-S1"></mediaResource><caption>Supplementary</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="graphical" xml:lang="en" xml:id="adhm201600334-abs-0001"><p><b>Trail polymerization enables a significant enhancement of the DhITACT system</b>. DhITACT‐Trail (DNA hydrogel formation by isothermal amplification of complementary targets trail polymerization) offers a robust diagnosis of target RNA strands in pseudo‐serum specimen. This system requires minimum liquid handling as compared to conventional analysis. In addition, a definitive diagnostic result can be achieved within 30 min by an optical detection.
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type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jae Bem</namePart><namePart type="family">You</namePart><affiliation>Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 34141, Daejeon, Republic of Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bo Ram</namePart><namePart type="family">Choi</namePart><affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ji Su</namePart><namePart type="family">Kim</namePart><affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hyun Kyung</namePart><namePart type="family">Lee</namePart><affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bora</namePart><namePart type="family">Jang</namePart><affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Han Saem</namePart><namePart type="family">Jeong</namePart><affiliation>College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kyuri</namePart><namePart 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Sciences, Ewha Womans University, 120‐750, Seoul, Republic of Korea</affiliation><affiliation>E-mail: hyukjin@ewha.ac.kr</affiliation><affiliation>E-mail: sgim@kaist.ac.kr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2016-09</dateIssued><dateCreated encoding="w3cdtf">2016-06-21</dateCreated><dateCaptured encoding="w3cdtf">2016-03-31</dateCaptured><copyrightDate encoding="w3cdtf">2016</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">4</extent><extent 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DhITACT‐Trail (DNA hydrogel formation by isothermal amplification of complementary targets trail polymerization) offers a robust diagnosis of target RNA strands in pseudo‐serum specimen. This system requires minimum liquid handling as compared to conventional analysis. In addition, a definitive diagnostic result can be achieved within 30 min by an optical detection.</abstract><relatedItem type="host"><titleInfo><title>Advanced Healthcare Materials</title></titleInfo><titleInfo type="abbreviated"><title>Adv. Healthcare Mater.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><note type="content"> As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re‐organized for online delivery, but are not copy‐edited or typeset. 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