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Real-time diagnosis of reactive oxygen species (ROS) in fresh sputum by electrochemical tracing; correlation between COVID-19 and viral-induced ROS in lung/respiratory epithelium during this pandemic.

Identifieur interne : 000926 ( Main/Corpus ); précédent : 000925; suivant : 000927

Real-time diagnosis of reactive oxygen species (ROS) in fresh sputum by electrochemical tracing; correlation between COVID-19 and viral-induced ROS in lung/respiratory epithelium during this pandemic.

Auteurs : Zohreh Sadat Miripour ; Ramin Sarrami-Forooshani ; Hassan Sanati ; Jalil Makarem ; Morteza Sanei Taheri ; Fatemeh Shojaeian ; Aida Hasanzadeh Eskafi ; Fereshteh Abbasvandi ; Naser Namdar ; Hadi Ghafari ; Parisa Aghaee ; Ashkan Zandi ; Mahsa Faramarzpour ; Meisam Hoseinyazdi ; Mahtab Tayebi ; Mohammad Abdolahad

Source :

RBID : pubmed:32729548

English descriptors

Abstract

COVID-19 is the shocking viral pandemics of this year which affected the health, economy, communications, and all aspects of social activities all over the world. Early diagnosis of this viral disease is very important since it can prevent lots of mortalities and care consumption. The functional similarities between COVID-19 and COVID-2 in inducing acute respiratory syndrome lightened our mind to find a diagnostic mechanism based on early traces of mitochondrial ROS overproduction as lung cells' dysfunctions induced by the virus. We designed a simple electrochemical sensor to selectively detect the intensity of ROS in the sputum sample (with a volume of less than 500 μl). Comparing the results of the sensor with clinical diagnostics of more than 140 normal and involved cases resulted in a response calibration with accuracy and sensitivity both 97%. Testing the sensor in more than 4 hospitals shed promising lights in ROS based real-time tracing of COVID-19 from the sputum sample.

DOI: 10.1016/j.bios.2020.112435
PubMed: 32729548
PubMed Central: PMC7341050

Links to Exploration step

pubmed:32729548

Le document en format XML

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<term>Biosensing Techniques (instrumentation)</term>
<term>Biosensing Techniques (methods)</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (virology)</term>
<term>Early Diagnosis (MeSH)</term>
<term>Electrochemical Techniques (instrumentation)</term>
<term>Electrochemical Techniques (methods)</term>
<term>Equipment Design (MeSH)</term>
<term>Female (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Lung (chemistry)</term>
<term>Lung (virology)</term>
<term>Male (MeSH)</term>
<term>Middle Aged (MeSH)</term>
<term>Pandemics (MeSH)</term>
<term>Pneumonia, Viral (diagnosis)</term>
<term>Pneumonia, Viral (virology)</term>
<term>Reactive Oxygen Species (analysis)</term>
<term>Sensitivity and Specificity (MeSH)</term>
<term>Sputum (chemistry)</term>
<term>Sputum (virology)</term>
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<div type="abstract" xml:lang="en">COVID-19 is the shocking viral pandemics of this year which affected the health, economy, communications, and all aspects of social activities all over the world. Early diagnosis of this viral disease is very important since it can prevent lots of mortalities and care consumption. The functional similarities between COVID-19 and COVID-2 in inducing acute respiratory syndrome lightened our mind to find a diagnostic mechanism based on early traces of mitochondrial ROS overproduction as lung cells' dysfunctions induced by the virus. We designed a simple electrochemical sensor to selectively detect the intensity of ROS in the sputum sample (with a volume of less than 500 μl). Comparing the results of the sensor with clinical diagnostics of more than 140 normal and involved cases resulted in a response calibration with accuracy and sensitivity both 97%. Testing the sensor in more than 4 hospitals shed promising lights in ROS based real-time tracing of COVID-19 from the sputum sample.</div>
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<AbstractText>COVID-19 is the shocking viral pandemics of this year which affected the health, economy, communications, and all aspects of social activities all over the world. Early diagnosis of this viral disease is very important since it can prevent lots of mortalities and care consumption. The functional similarities between COVID-19 and COVID-2 in inducing acute respiratory syndrome lightened our mind to find a diagnostic mechanism based on early traces of mitochondrial ROS overproduction as lung cells' dysfunctions induced by the virus. We designed a simple electrochemical sensor to selectively detect the intensity of ROS in the sputum sample (with a volume of less than 500 μl). Comparing the results of the sensor with clinical diagnostics of more than 140 normal and involved cases resulted in a response calibration with accuracy and sensitivity both 97%. Testing the sensor in more than 4 hospitals shed promising lights in ROS based real-time tracing of COVID-19 from the sputum sample.</AbstractText>
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