Routine blood analysis greatly reduces the false-negative rate of RT-PCR testing for Covid-19.
Identifieur interne : 000239 ( Main/Corpus ); précédent : 000238; suivant : 000240Routine blood analysis greatly reduces the false-negative rate of RT-PCR testing for Covid-19.
Auteurs : Davide Ferrari ; Eleonora Sabetta ; Daniele Ceriotti ; Andrea Motta ; Marta Strollo ; Giuseppe Banfi ; Massimo LocatelliSource :
- Acta bio-medica : Atenei Parmensis [ 2531-6745 ] ; 2020.
English descriptors
- KwdEn :
- Adult (MeSH), Aged (MeSH), Aged, 80 and over (MeSH), Aspartate Aminotransferases (blood), Betacoronavirus (genetics), Biomarkers (blood), Coronavirus Infections (blood), Coronavirus Infections (diagnosis), Coronavirus Infections (epidemiology), Diagnosis, Differential (MeSH), False Negative Reactions (MeSH), Female (MeSH), Follow-Up Studies (MeSH), Hematologic Tests (methods), Humans (MeSH), Italy (epidemiology), L-Lactate Dehydrogenase (blood), Male (MeSH), Middle Aged (MeSH), Pandemics (MeSH), Pneumonia, Viral (blood), Pneumonia, Viral (diagnosis), Pneumonia, Viral (epidemiology), RNA, Viral (analysis), Real-Time Polymerase Chain Reaction (methods), Reproducibility of Results (MeSH), Retrospective Studies (MeSH).
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , blood : Aspartate Aminotransferases, Biomarkers, L-Lactate Dehydrogenase.
- geographic , epidemiology : Italy.
- blood : Coronavirus Infections, Pneumonia, Viral.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- epidemiology : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus.
- methods : Hematologic Tests, Real-Time Polymerase Chain Reaction.
- Adult, Aged, Aged, 80 and over, Diagnosis, Differential, False Negative Reactions, Female, Follow-Up Studies, Humans, Male, Middle Aged, Pandemics, Reproducibility of Results, Retrospective Studies.
Abstract
BACKGROUND
The COVID-19 outbreak is now a pandemic disease reaching as much as 210 countries worldwide with more than 2.5 million infected people and nearly 200.000 deaths. Amplification of viral RNA by RT-PCR represents the gold standard for confirmation of infection, yet it showed false-negative rates as large as 15-20% which may jeopardize the effect of the restrictive measures taken by governments. We previously showed that several hematological parameters were significantly different between COVID-19 positive and negative patients. Among them aspartate aminotransferase and lactate dehydrogenase had predictive values as large as 90%. Thus a combination of RT-PCR and blood tests could reduce the false-negative rate of the genetic test.
METHODS
We retrospectively analyzed 24 patients showing multiple and inconsistent RT-PCR, test during their first hospitalization period, and compared the genetic tests results with their AST and LDH levels.
RESULTS
We showed that when considering the hematological parameters, the RT-PCR false-negative rates were reduced by almost 4-fold.
CONCLUSIONS
The study represents a preliminary work aiming at the development of strategies that, by combining RT-PCR tests with routine blood tests, will lower or even abolish the rate of RT-PCR false-negative results and thus will identify, with high accuracy, patients infected by COVID-19.
DOI: 10.23750/abm.v91i3.9843
PubMed: 32921701
Links to Exploration step
pubmed:32921701Le document en format XML
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<author><name sortKey="Ferrari, Davide" sort="Ferrari, Davide" uniqKey="Ferrari D" first="Davide" last="Ferrari">Davide Ferrari</name>
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<author><name sortKey="Sabetta, Eleonora" sort="Sabetta, Eleonora" uniqKey="Sabetta E" first="Eleonora" last="Sabetta">Eleonora Sabetta</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Routine blood analysis greatly reduces the false-negative rate of RT-PCR testing for Covid-19.</title>
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<term>Aspartate Aminotransferases (blood)</term>
<term>Betacoronavirus (genetics)</term>
<term>Biomarkers (blood)</term>
<term>Coronavirus Infections (blood)</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (epidemiology)</term>
<term>Diagnosis, Differential (MeSH)</term>
<term>False Negative Reactions (MeSH)</term>
<term>Female (MeSH)</term>
<term>Follow-Up Studies (MeSH)</term>
<term>Hematologic Tests (methods)</term>
<term>Humans (MeSH)</term>
<term>Italy (epidemiology)</term>
<term>L-Lactate Dehydrogenase (blood)</term>
<term>Male (MeSH)</term>
<term>Middle Aged (MeSH)</term>
<term>Pandemics (MeSH)</term>
<term>Pneumonia, Viral (blood)</term>
<term>Pneumonia, Viral (diagnosis)</term>
<term>Pneumonia, Viral (epidemiology)</term>
<term>RNA, Viral (analysis)</term>
<term>Real-Time Polymerase Chain Reaction (methods)</term>
<term>Reproducibility of Results (MeSH)</term>
<term>Retrospective Studies (MeSH)</term>
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<term>Biomarkers</term>
<term>L-Lactate Dehydrogenase</term>
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<term>Pneumonia, Viral</term>
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<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term>
<term>Pneumonia, Viral</term>
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<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Pneumonia, Viral</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betacoronavirus</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Hematologic Tests</term>
<term>Real-Time Polymerase Chain Reaction</term>
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<term>Aged, 80 and over</term>
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<term>Follow-Up Studies</term>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>The COVID-19 outbreak is now a pandemic disease reaching as much as 210 countries worldwide with more than 2.5 million infected people and nearly 200.000 deaths. Amplification of viral RNA by RT-PCR represents the gold standard for confirmation of infection, yet it showed false-negative rates as large as 15-20% which may jeopardize the effect of the restrictive measures taken by governments. We previously showed that several hematological parameters were significantly different between COVID-19 positive and negative patients. Among them aspartate aminotransferase and lactate dehydrogenase had predictive values as large as 90%. Thus a combination of RT-PCR and blood tests could reduce the false-negative rate of the genetic test.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODS</b>
</p>
<p>We retrospectively analyzed 24 patients showing multiple and inconsistent RT-PCR, test during their first hospitalization period, and compared the genetic tests results with their AST and LDH levels.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>We showed that when considering the hematological parameters, the RT-PCR false-negative rates were reduced by almost 4-fold.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>The study represents a preliminary work aiming at the development of strategies that, by combining RT-PCR tests with routine blood tests, will lower or even abolish the rate of RT-PCR false-negative results and thus will identify, with high accuracy, patients infected by COVID-19.</p>
</div>
</front>
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<Abstract><AbstractText Label="BACKGROUND">The COVID-19 outbreak is now a pandemic disease reaching as much as 210 countries worldwide with more than 2.5 million infected people and nearly 200.000 deaths. Amplification of viral RNA by RT-PCR represents the gold standard for confirmation of infection, yet it showed false-negative rates as large as 15-20% which may jeopardize the effect of the restrictive measures taken by governments. We previously showed that several hematological parameters were significantly different between COVID-19 positive and negative patients. Among them aspartate aminotransferase and lactate dehydrogenase had predictive values as large as 90%. Thus a combination of RT-PCR and blood tests could reduce the false-negative rate of the genetic test.</AbstractText>
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