Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines.
Identifieur interne : 000609 ( Main/Exploration ); précédent : 000608; suivant : 000610Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines.
Auteurs : Lucia Grenga [France] ; Fabrice Gallais [France] ; Olivier Pible [France] ; Jean-Charles Gaillard [France] ; Duarte Gouveia [France] ; Hélène Batina [France] ; Niza Bazaline [France] ; Sylvie Ruat [France] ; Karen Culotta [France] ; Guylaine Miotello [France] ; Stéphanie Debroas [France] ; Marie-Anne Roncato [France] ; Gérard Steinmetz [France] ; Charlotte Foissard [France] ; Anne Desplan [France] ; Béatrice Alpha-Bazin [France] ; Christine Almunia [France] ; Fabienne Gas [France] ; Laurent Bellanger [France] ; Jean Armengaud [France]Source :
- Emerging microbes & infections [ 2222-1751 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Antigènes viraux.
- immunologie : Antigènes viraux, Betacoronavirus, Vaccins antiviraux, Virion.
- méthodes : Protéomique.
- Animaux, Cellules Vero, Spectrométrie de masse en tandem.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Antigens, Viral.
- chemical , immunology : Antigens, Viral, Viral Vaccines.
- immunology : Betacoronavirus, Virion.
- methods : Proteomics.
- Animals, Chlorocebus aethiops, Tandem Mass Spectrometry, Vero Cells.
Abstract
Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and is continuing to spread rapidly around the globe. No effective vaccine is currently available to prevent COVID-19, and intense efforts are being invested worldwide into vaccine development. In this context, all technology platforms must overcome several challenges resulting from the use of an incompletely characterized new virus. These include finding the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles. Here, we describe a shotgun tandem mass spectrometry workflow, the data produced can be used to guide optimization of the conditions for viral amplification. In parallel, we analysed the changes occurring in the host cell proteome following SARS-CoV-2 infection to glean information on the biological processes modulated by the virus that could be further explored as potential drug targets to deal with the pandemic.
DOI: 10.1080/22221751.2020.1791737
PubMed: 32619390
PubMed Central: PMC7473198
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Batina, Helene" sort="Batina, Helene" uniqKey="Batina H" first="Hélène" last="Batina">Hélène Batina</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Bazaline, Niza" sort="Bazaline, Niza" uniqKey="Bazaline N" first="Niza" last="Bazaline">Niza Bazaline</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Ruat, Sylvie" sort="Ruat, Sylvie" uniqKey="Ruat S" first="Sylvie" last="Ruat">Sylvie Ruat</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Culotta, Karen" sort="Culotta, Karen" uniqKey="Culotta K" first="Karen" last="Culotta">Karen Culotta</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Miotello, Guylaine" sort="Miotello, Guylaine" uniqKey="Miotello G" first="Guylaine" last="Miotello">Guylaine Miotello</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Debroas, Stephanie" sort="Debroas, Stephanie" uniqKey="Debroas S" first="Stéphanie" last="Debroas">Stéphanie Debroas</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Roncato, Marie Anne" sort="Roncato, Marie Anne" uniqKey="Roncato M" first="Marie-Anne" last="Roncato">Marie-Anne Roncato</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Steinmetz, Gerard" sort="Steinmetz, Gerard" uniqKey="Steinmetz G" first="Gérard" last="Steinmetz">Gérard Steinmetz</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Foissard, Charlotte" sort="Foissard, Charlotte" uniqKey="Foissard C" first="Charlotte" last="Foissard">Charlotte Foissard</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Desplan, Anne" sort="Desplan, Anne" uniqKey="Desplan A" first="Anne" last="Desplan">Anne Desplan</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Alpha Bazin, Beatrice" sort="Alpha Bazin, Beatrice" uniqKey="Alpha Bazin B" first="Béatrice" last="Alpha-Bazin">Béatrice Alpha-Bazin</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Almunia, Christine" sort="Almunia, Christine" uniqKey="Almunia C" first="Christine" last="Almunia">Christine Almunia</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Gas, Fabienne" sort="Gas, Fabienne" uniqKey="Gas F" first="Fabienne" last="Gas">Fabienne Gas</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Bellanger, Laurent" sort="Bellanger, Laurent" uniqKey="Bellanger L" first="Laurent" last="Bellanger">Laurent Bellanger</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Armengaud, Jean" sort="Armengaud, Jean" uniqKey="Armengaud J" first="Jean" last="Armengaud">Jean Armengaud</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32619390</idno><idno type="pmid">32619390</idno><idno type="doi">10.1080/22221751.2020.1791737</idno><idno type="pmc">PMC7473198</idno><idno type="wicri:Area/Main/Corpus">000B14</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000B14</idno><idno type="wicri:Area/Main/Curation">000B14</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000B14</idno><idno type="wicri:Area/Main/Exploration">000B14</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines.</title><author><name sortKey="Grenga, Lucia" sort="Grenga, Lucia" uniqKey="Grenga L" first="Lucia" last="Grenga">Lucia Grenga</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Gallais, Fabrice" sort="Gallais, Fabrice" uniqKey="Gallais F" first="Fabrice" last="Gallais">Fabrice Gallais</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Pible, Olivier" sort="Pible, Olivier" uniqKey="Pible O" first="Olivier" last="Pible">Olivier Pible</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Gaillard, Jean Charles" sort="Gaillard, Jean Charles" uniqKey="Gaillard J" first="Jean-Charles" last="Gaillard">Jean-Charles Gaillard</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Gouveia, Duarte" sort="Gouveia, Duarte" uniqKey="Gouveia D" first="Duarte" last="Gouveia">Duarte Gouveia</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Batina, Helene" sort="Batina, Helene" uniqKey="Batina H" first="Hélène" last="Batina">Hélène Batina</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Bazaline, Niza" sort="Bazaline, Niza" uniqKey="Bazaline N" first="Niza" last="Bazaline">Niza Bazaline</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Ruat, Sylvie" sort="Ruat, Sylvie" uniqKey="Ruat S" first="Sylvie" last="Ruat">Sylvie Ruat</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Culotta, Karen" sort="Culotta, Karen" uniqKey="Culotta K" first="Karen" last="Culotta">Karen Culotta</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Miotello, Guylaine" sort="Miotello, Guylaine" uniqKey="Miotello G" first="Guylaine" last="Miotello">Guylaine Miotello</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Debroas, Stephanie" sort="Debroas, Stephanie" uniqKey="Debroas S" first="Stéphanie" last="Debroas">Stéphanie Debroas</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Roncato, Marie Anne" sort="Roncato, Marie Anne" uniqKey="Roncato M" first="Marie-Anne" last="Roncato">Marie-Anne Roncato</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Steinmetz, Gerard" sort="Steinmetz, Gerard" uniqKey="Steinmetz G" first="Gérard" last="Steinmetz">Gérard Steinmetz</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Foissard, Charlotte" sort="Foissard, Charlotte" uniqKey="Foissard C" first="Charlotte" last="Foissard">Charlotte Foissard</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Desplan, Anne" sort="Desplan, Anne" uniqKey="Desplan A" first="Anne" last="Desplan">Anne Desplan</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Alpha Bazin, Beatrice" sort="Alpha Bazin, Beatrice" uniqKey="Alpha Bazin B" first="Béatrice" last="Alpha-Bazin">Béatrice Alpha-Bazin</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Almunia, Christine" sort="Almunia, Christine" uniqKey="Almunia C" first="Christine" last="Almunia">Christine Almunia</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Gas, Fabienne" sort="Gas, Fabienne" uniqKey="Gas F" first="Fabienne" last="Gas">Fabienne Gas</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Bellanger, Laurent" sort="Bellanger, Laurent" uniqKey="Bellanger L" first="Laurent" last="Bellanger">Laurent Bellanger</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author><author><name sortKey="Armengaud, Jean" sort="Armengaud, Jean" uniqKey="Armengaud J" first="Jean" last="Armengaud">Jean Armengaud</name><affiliation wicri:level="1"><nlm:affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze</wicri:regionArea><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion><wicri:noRegion>SPI Bagnols-sur-Cèze</wicri:noRegion></affiliation></author></analytic><series><title level="j">Emerging microbes & infections</title><idno type="eISSN">2222-1751</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Antigens, Viral (biosynthesis)</term><term>Antigens, Viral (immunology)</term><term>Betacoronavirus (immunology)</term><term>Chlorocebus aethiops (MeSH)</term><term>Proteomics (methods)</term><term>Tandem Mass Spectrometry (MeSH)</term><term>Vero Cells (MeSH)</term><term>Viral Vaccines (immunology)</term><term>Virion (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Antigènes viraux (biosynthèse)</term><term>Antigènes viraux (immunologie)</term><term>Betacoronavirus (immunologie)</term><term>Cellules Vero (MeSH)</term><term>Protéomique (méthodes)</term><term>Spectrométrie de masse en tandem (MeSH)</term><term>Vaccins antiviraux (immunologie)</term><term>Virion (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Antigènes viraux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term><term>Betacoronavirus</term><term>Vaccins antiviraux</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Betacoronavirus</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Protéomique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Tandem Mass Spectrometry</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Spectrométrie de masse en tandem</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and is continuing to spread rapidly around the globe. No effective vaccine is currently available to prevent COVID-19, and intense efforts are being invested worldwide into vaccine development. In this context, all technology platforms must overcome several challenges resulting from the use of an incompletely characterized new virus. These include finding the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles. Here, we describe a shotgun tandem mass spectrometry workflow, the data produced can be used to guide optimization of the conditions for viral amplification. In parallel, we analysed the changes occurring in the host cell proteome following SARS-CoV-2 infection to glean information on the biological processes modulated by the virus that could be further explored as potential drug targets to deal with the pandemic.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32619390</PMID><DateCompleted><Year>2020</Year><Month>08</Month><Day>04</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">2222-1751</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Emerging microbes & infections</Title><ISOAbbreviation>Emerg Microbes Infect</ISOAbbreviation></Journal><ArticleTitle>Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines.</ArticleTitle><Pagination><MedlinePgn>1712-1721</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/22221751.2020.1791737</ELocationID><Abstract><AbstractText>Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and is continuing to spread rapidly around the globe. No effective vaccine is currently available to prevent COVID-19, and intense efforts are being invested worldwide into vaccine development. In this context, all technology platforms must overcome several challenges resulting from the use of an incompletely characterized new virus. These include finding the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles. Here, we describe a shotgun tandem mass spectrometry workflow, the data produced can be used to guide optimization of the conditions for viral amplification. In parallel, we analysed the changes occurring in the host cell proteome following SARS-CoV-2 infection to glean information on the biological processes modulated by the virus that could be further explored as potential drug targets to deal with the pandemic.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Grenga</LastName><ForeName>Lucia</ForeName><Initials>L</Initials><Identifier Source="ORCID">https://orcid.org/0000-0001-5560-1717</Identifier><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gallais</LastName><ForeName>Fabrice</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pible</LastName><ForeName>Olivier</ForeName><Initials>O</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-4398-6730</Identifier><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gaillard</LastName><ForeName>Jean-Charles</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gouveia</LastName><ForeName>Duarte</ForeName><Initials>D</Initials><Identifier Source="ORCID">https://orcid.org/0000-0001-5578-3370</Identifier><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Batina</LastName><ForeName>Hélène</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bazaline</LastName><ForeName>Niza</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ruat</LastName><ForeName>Sylvie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Culotta</LastName><ForeName>Karen</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miotello</LastName><ForeName>Guylaine</ForeName><Initials>G</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-2398-1249</Identifier><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Debroas</LastName><ForeName>Stéphanie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roncato</LastName><ForeName>Marie-Anne</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Steinmetz</LastName><ForeName>Gérard</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Foissard</LastName><ForeName>Charlotte</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Desplan</LastName><ForeName>Anne</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alpha-Bazin</LastName><ForeName>Béatrice</ForeName><Initials>B</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-9334-4392</Identifier><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Almunia</LastName><ForeName>Christine</ForeName><Initials>C</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-8749-5557</Identifier><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gas</LastName><ForeName>Fabienne</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bellanger</LastName><ForeName>Laurent</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Armengaud</LastName><ForeName>Jean</ForeName><Initials>J</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-1589-445X</Identifier><AffiliationInfo><Affiliation>Département Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI Bagnols-sur-Cèze, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Emerg Microbes Infect</MedlineTA><NlmUniqueID>101594885</NlmUniqueID><ISSNLinking>2222-1751</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014765">Viral Vaccines</NameOfSubstance></Chemical></ChemicalList><SupplMeshList><SupplMeshName Type="Organism" UI="C000656484">severe acute respiratory syndrome coronavirus 2</SupplMeshName></SupplMeshList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000073640" MajorTopicYN="N">Betacoronavirus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014765" MajorTopicYN="N">Viral Vaccines</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014771" MajorTopicYN="N">Virion</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">COVID-19</Keyword><Keyword MajorTopicYN="N">SARS-CoV-2</Keyword><Keyword MajorTopicYN="N">host response</Keyword><Keyword MajorTopicYN="N">infection kinetics</Keyword><Keyword MajorTopicYN="N">mass spectrometry</Keyword><Keyword MajorTopicYN="N">proteomics</Keyword><Keyword MajorTopicYN="N">vaccine</Keyword><Keyword MajorTopicYN="N">viral protein detection</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32619390</ArticleId><ArticleId IdType="doi">10.1080/22221751.2020.1791737</ArticleId><ArticleId IdType="pmc">PMC7473198</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nature. 2020 Mar;579(7798):270-273</Citation><ArticleIdList><ArticleId IdType="pubmed">32015507</ArticleId></ArticleIdList></Reference><Reference><Citation>Antimicrob Agents Chemother. 2020 Jun 23;64(7):</Citation><ArticleIdList><ArticleId IdType="pubmed">32366720</ArticleId></ArticleIdList></Reference><Reference><Citation>Antiviral Res. 2020 Jun;178:104792</Citation><ArticleIdList><ArticleId IdType="pubmed">32272173</ArticleId></ArticleIdList></Reference><Reference><Citation>mSphere. 2020 Mar 18;5(2):</Citation><ArticleIdList><ArticleId IdType="pubmed">32188753</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2020 Aug 19;:</Citation><ArticleIdList><ArticleId IdType="pubmed">32817221</ArticleId></ArticleIdList></Reference><Reference><Citation>J Korean Med Sci. 2020 Feb 24;35(7):e84</Citation><ArticleIdList><ArticleId IdType="pubmed">32080990</ArticleId></ArticleIdList></Reference><Reference><Citation>Syst Appl Microbiol. 2015 Jun;38(4):246-57</Citation><ArticleIdList><ArticleId IdType="pubmed">25933927</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2014 Mar 25;9(3):e92974</Citation><ArticleIdList><ArticleId IdType="pubmed">24667817</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol. 2020 Aug;22(8):2996-3004</Citation><ArticleIdList><ArticleId IdType="pubmed">32133743</ArticleId></ArticleIdList></Reference><Reference><Citation>Trop Med Int Health. 2020 Mar;25(3):278-280</Citation><ArticleIdList><ArticleId IdType="pubmed">32052514</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450</Citation><ArticleIdList><ArticleId IdType="pubmed">30395289</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2014;1197:275-85</Citation><ArticleIdList><ArticleId IdType="pubmed">25172287</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2020 Jul;583(7816):469-472</Citation><ArticleIdList><ArticleId IdType="pubmed">32408336</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Dis Ther. 2020 Apr 23;:1-20</Citation><ArticleIdList><ArticleId IdType="pubmed">32328406</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Proteomics. 2005 Jul;4(7):902-13</Citation><ArticleIdList><ArticleId IdType="pubmed">15784933</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Microbes Infect. 2020 Dec;9(1):275-277</Citation><ArticleIdList><ArticleId IdType="pubmed">32005086</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2011 Oct 03;9(12):889-93</Citation><ArticleIdList><ArticleId IdType="pubmed">21963800</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Med. 2020 Jul 28;12(1):68</Citation><ArticleIdList><ArticleId IdType="pubmed">32723359</ArticleId></ArticleIdList></Reference><Reference><Citation>Aging (Albany NY). 2020 Jun 23;12(12):11277-11286</Citation><ArticleIdList><ArticleId IdType="pubmed">32575076</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2020 Jan;25(3):</Citation><ArticleIdList><ArticleId IdType="pubmed">31992387</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteomics. 2015 Jun;15(11):1819-28</Citation><ArticleIdList><ArticleId IdType="pubmed">25604190</ArticleId></ArticleIdList></Reference><Reference><Citation>Brief Bioinform. 2018 May 1;19(3):425-436</Citation><ArticleIdList><ArticleId IdType="pubmed">28065917</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Drug Discov. 2020 May;19(5):305-306</Citation><ArticleIdList><ArticleId IdType="pubmed">32273591</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Immunol. 2009 Jan;46(3):393-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19038458</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2019 Apr 3;10(1):1523</Citation><ArticleIdList><ArticleId IdType="pubmed">30944313</ArticleId></ArticleIdList></Reference><Reference><Citation>J Microbiol Biotechnol. 2019 Nov 28;29(11):1817-1829</Citation><ArticleIdList><ArticleId IdType="pubmed">31546302</ArticleId></ArticleIdList></Reference><Reference><Citation>Bull Acad Natl Med. 2008 Mar;192(3):511-8; discussion 518-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18819697</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteome Res. 2017 Jun 2;16(6):2113-2120</Citation><ArticleIdList><ArticleId IdType="pubmed">28506058</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2016 Feb;13(2):151-7</Citation><ArticleIdList><ArticleId IdType="pubmed">26689262</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteomics. 2014 Jun;14(11):1403-23</Citation><ArticleIdList><ArticleId IdType="pubmed">24610701</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteomics. 2020 Jul;20(14):e2000107</Citation><ArticleIdList><ArticleId IdType="pubmed">32462744</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Grenga, Lucia" sort="Grenga, Lucia" uniqKey="Grenga L" first="Lucia" last="Grenga">Lucia Grenga</name></noRegion><name sortKey="Almunia, Christine" sort="Almunia, Christine" uniqKey="Almunia C" first="Christine" last="Almunia">Christine Almunia</name><name sortKey="Alpha Bazin, Beatrice" sort="Alpha Bazin, 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M" first="Marie-Anne" last="Roncato">Marie-Anne Roncato</name><name sortKey="Ruat, Sylvie" sort="Ruat, Sylvie" uniqKey="Ruat S" first="Sylvie" last="Ruat">Sylvie Ruat</name><name sortKey="Steinmetz, Gerard" sort="Steinmetz, Gerard" uniqKey="Steinmetz G" first="Gérard" last="Steinmetz">Gérard Steinmetz</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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