How to perform a nasopharyngeal swab in adults and children in the COVID-19 era.
Identifieur interne : 000A37 ( Main/Curation ); précédent : 000A36; suivant : 000A38How to perform a nasopharyngeal swab in adults and children in the COVID-19 era.
Auteurs : S. Pondaven-Letourmy [France] ; F. Alvin [France] ; Y. Boumghit [France] ; F. Simon [France]Source :
- European annals of otorhinolaryngology, head and neck diseases [ 1879-730X ] ; 2020.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Adulte (MeSH), Betacoronavirus (génétique), Betacoronavirus (isolement et purification), Enfant (MeSH), Humains (MeSH), Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Pandémies (MeSH), Partie nasale du pharynx (virologie), Pneumopathie virale (diagnostic), Pneumopathie virale (virologie), RT-PCR (MeSH), Techniques de laboratoire clinique (méthodes).
- MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus, Pneumopathie virale.
- génétique : Betacoronavirus.
- isolement et purification : Betacoronavirus.
- méthodes : Techniques de laboratoire clinique.
- virologie : Infections à coronavirus, Partie nasale du pharynx, Pneumopathie virale.
- Adulte, Enfant, Humains, Pandémies, RT-PCR.
English descriptors
- KwdEn :
- Adult (MeSH), Betacoronavirus (genetics), Betacoronavirus (isolation & purification), Child (MeSH), Clinical Laboratory Techniques (methods), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Humans (MeSH), Nasopharynx (virology), Pandemics (MeSH), Pneumonia, Viral (diagnosis), Pneumonia, Viral (virology), RNA, Viral (analysis), Reverse Transcriptase Polymerase Chain Reaction (MeSH).
- MESH :
- chemical , analysis : RNA, Viral.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus.
- isolation & purification : Betacoronavirus.
- methods : Clinical Laboratory Techniques.
- virology : Coronavirus Infections, Nasopharynx, Pneumonia, Viral.
- Adult, Child, Humans, Pandemics, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
The nasopharyngeal swab is currently the main testing method used to diagnose COVID-19. The principle is to collect respiratory cells infected by the virus and to use the RT-PCR (Reverse Transcription - Polymerase Chain Reaction) technique to detect the RNA of the virus. The false negative rate is high, about 30%, which can mainly be explained by an incorrect execution of the technique may increase the false negative rate and decrease the test's sensitivity. The aim of this note is to help healthcare providers to perform this test correctly in adults and children.
DOI: 10.1016/j.anorl.2020.06.001
PubMed: 32646750
PubMed Central: PMC7274641
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pubmed:32646750Le document en format XML
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<term>Child (MeSH)</term>
<term>Clinical Laboratory Techniques (methods)</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (virology)</term>
<term>Humans (MeSH)</term>
<term>Nasopharynx (virology)</term>
<term>Pandemics (MeSH)</term>
<term>Pneumonia, Viral (diagnosis)</term>
<term>Pneumonia, Viral (virology)</term>
<term>RNA, Viral (analysis)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
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<term>Betacoronavirus (isolement et purification)</term>
<term>Enfant (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Pandémies (MeSH)</term>
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<term>Pneumopathie virale (virologie)</term>
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<front><div type="abstract" xml:lang="en">The nasopharyngeal swab is currently the main testing method used to diagnose COVID-19. The principle is to collect respiratory cells infected by the virus and to use the RT-PCR (Reverse Transcription - Polymerase Chain Reaction) technique to detect the RNA of the virus. The false negative rate is high, about 30%, which can mainly be explained by an incorrect execution of the technique may increase the false negative rate and decrease the test's sensitivity. The aim of this note is to help healthcare providers to perform this test correctly in adults and children.</div>
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