Hydroxychloroquine prophylaxis and treatment is ineffective in macaque and hamster SARS-CoV-2 disease models.
Identifieur interne : 000964 ( Main/Corpus ); précédent : 000963; suivant : 000965Hydroxychloroquine prophylaxis and treatment is ineffective in macaque and hamster SARS-CoV-2 disease models.
Auteurs : Kyle Rosenke ; Michael A. Jarvis ; Friederike Feldmann ; Benjamin Schwarz ; Atsushi Okumura ; Jamie Lovaglio ; Greg Saturday ; Patrick W. Hanley ; Kimberly Meade-White ; Brandi N. Williamson ; Frederick Hansen ; Lizette Perez-Perez ; Shanna Leventhal ; Tsing-Lee Tang-Huau ; Julie Callison ; Elaine Haddock ; Kaitlin A. Stromberg ; Dana Scott ; Graham Sewell ; Catharine M. Bosio ; David Hawman ; Emmie De Wit ; Heinz FeldmannSource :
- JCI insight [ 2379-3708 ] ; 2020.
English descriptors
- KwdEn :
- Animals (MeSH), Antiviral Agents (therapeutic use), COVID-19 (drug therapy), COVID-19 (pathology), COVID-19 (prevention & control), COVID-19 (therapy), Chlorocebus aethiops (MeSH), Cricetinae (MeSH), Cytokines (metabolism), Disease Models, Animal (MeSH), Drug Evaluation, Preclinical (MeSH), Humans (MeSH), Hydroxychloroquine (therapeutic use), Lung (pathology), Lung (virology), Macaca mulatta (MeSH), Male (MeSH), SARS-CoV-2 (drug effects), Treatment Outcome (MeSH), Vero Cells (MeSH), Viral Load (drug effects), Virus Replication (drug effects), Virus Shedding (drug effects).
- MESH :
- chemical , metabolism : Cytokines.
- chemical , therapeutic use : Antiviral Agents, Hydroxychloroquine.
- drug effects : SARS-CoV-2, Viral Load, Virus Replication, Virus Shedding.
- drug therapy : COVID-19.
- pathology : COVID-19, Lung.
- prevention & control : COVID-19.
- therapy : COVID-19.
- virology : Lung.
- Animals, Chlorocebus aethiops, Cricetinae, Disease Models, Animal, Drug Evaluation, Preclinical, Humans, Macaca mulatta, Male, Treatment Outcome, Vero Cells.
Abstract
We remain largely without effective prophylactic/therapeutic interventions for COVID-19. Although many human COVID-19 clinical trials are ongoing, there remains a deficiency of supportive preclinical drug efficacy studies to help guide decisions. Here we assessed the prophylactic/therapeutic efficacy of hydroxychloroquine (HCQ), a drug of interest for COVID-19 management, in 2 animal disease models. The standard human malaria HCQ prophylaxis (6.5 mg/kg given weekly) and treatment (6.5 mg/kg given daily) did not significantly benefit clinical outcome, nor did it reduce SARS-CoV-2 replication/shedding in the upper and lower respiratory tract in the rhesus macaque disease model. Similarly, when used for prophylaxis or treatment, neither the standard human malaria dose (6.5 mg/kg) nor a high dose (50 mg/kg) of HCQ had any beneficial effect on clinical disease or SARS-CoV-2 kinetics (replication/shedding) in the Syrian hamster disease model. Results from these 2 preclinical animal models may prove helpful in guiding clinical use of HCQ for prophylaxis/treatment of COVID-19.
DOI: 10.1172/jci.insight.143174
PubMed: 33090972
PubMed Central: PMC7714406
Links to Exploration step
pubmed:33090972Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Hydroxychloroquine prophylaxis and treatment is ineffective in macaque and hamster SARS-CoV-2 disease models.</title>
<author><name sortKey="Rosenke, Kyle" sort="Rosenke, Kyle" uniqKey="Rosenke K" first="Kyle" last="Rosenke">Kyle Rosenke</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Jarvis, Michael A" sort="Jarvis, Michael A" uniqKey="Jarvis M" first="Michael A" last="Jarvis">Michael A. Jarvis</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>University of Plymouth, Plymouth, Devon, United Kingdom; The Vaccine Group Ltd, Plymouth, Devon, United Kingdom.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name>
<affiliation><nlm:affiliation>Rocky Mountain Veterinary Branch and.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Schwarz, Benjamin" sort="Schwarz, Benjamin" uniqKey="Schwarz B" first="Benjamin" last="Schwarz">Benjamin Schwarz</name>
<affiliation><nlm:affiliation>Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Okumura, Atsushi" sort="Okumura, Atsushi" uniqKey="Okumura A" first="Atsushi" last="Okumura">Atsushi Okumura</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Lovaglio, Jamie" sort="Lovaglio, Jamie" uniqKey="Lovaglio J" first="Jamie" last="Lovaglio">Jamie Lovaglio</name>
<affiliation><nlm:affiliation>Rocky Mountain Veterinary Branch and.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Saturday, Greg" sort="Saturday, Greg" uniqKey="Saturday G" first="Greg" last="Saturday">Greg Saturday</name>
<affiliation><nlm:affiliation>Rocky Mountain Veterinary Branch and.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hanley, Patrick W" sort="Hanley, Patrick W" uniqKey="Hanley P" first="Patrick W" last="Hanley">Patrick W. Hanley</name>
<affiliation><nlm:affiliation>Rocky Mountain Veterinary Branch and.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Meade White, Kimberly" sort="Meade White, Kimberly" uniqKey="Meade White K" first="Kimberly" last="Meade-White">Kimberly Meade-White</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Williamson, Brandi N" sort="Williamson, Brandi N" uniqKey="Williamson B" first="Brandi N" last="Williamson">Brandi N. Williamson</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hansen, Frederick" sort="Hansen, Frederick" uniqKey="Hansen F" first="Frederick" last="Hansen">Frederick Hansen</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Perez Perez, Lizette" sort="Perez Perez, Lizette" uniqKey="Perez Perez L" first="Lizette" last="Perez-Perez">Lizette Perez-Perez</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Leventhal, Shanna" sort="Leventhal, Shanna" uniqKey="Leventhal S" first="Shanna" last="Leventhal">Shanna Leventhal</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Tang Huau, Tsing Lee" sort="Tang Huau, Tsing Lee" uniqKey="Tang Huau T" first="Tsing-Lee" last="Tang-Huau">Tsing-Lee Tang-Huau</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Callison, Julie" sort="Callison, Julie" uniqKey="Callison J" first="Julie" last="Callison">Julie Callison</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Stromberg, Kaitlin A" sort="Stromberg, Kaitlin A" uniqKey="Stromberg K" first="Kaitlin A" last="Stromberg">Kaitlin A. Stromberg</name>
<affiliation><nlm:affiliation>Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name>
<affiliation><nlm:affiliation>Rocky Mountain Veterinary Branch and.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Sewell, Graham" sort="Sewell, Graham" uniqKey="Sewell G" first="Graham" last="Sewell">Graham Sewell</name>
<affiliation><nlm:affiliation>Leicester School of Pharmacy, De Montfort University, Leicester, United Kingdom.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Bosio, Catharine M" sort="Bosio, Catharine M" uniqKey="Bosio C" first="Catharine M" last="Bosio">Catharine M. Bosio</name>
<affiliation><nlm:affiliation>Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hawman, David" sort="Hawman, David" uniqKey="Hawman D" first="David" last="Hawman">David Hawman</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2020">2020</date>
<idno type="RBID">pubmed:33090972</idno>
<idno type="pmid">33090972</idno>
<idno type="doi">10.1172/jci.insight.143174</idno>
<idno type="pmc">PMC7714406</idno>
<idno type="wicri:Area/Main/Corpus">000964</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000964</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Hydroxychloroquine prophylaxis and treatment is ineffective in macaque and hamster SARS-CoV-2 disease models.</title>
<author><name sortKey="Rosenke, Kyle" sort="Rosenke, Kyle" uniqKey="Rosenke K" first="Kyle" last="Rosenke">Kyle Rosenke</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Jarvis, Michael A" sort="Jarvis, Michael A" uniqKey="Jarvis M" first="Michael A" last="Jarvis">Michael A. Jarvis</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>University of Plymouth, Plymouth, Devon, United Kingdom; The Vaccine Group Ltd, Plymouth, Devon, United Kingdom.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name>
<affiliation><nlm:affiliation>Rocky Mountain Veterinary Branch and.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Schwarz, Benjamin" sort="Schwarz, Benjamin" uniqKey="Schwarz B" first="Benjamin" last="Schwarz">Benjamin Schwarz</name>
<affiliation><nlm:affiliation>Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Okumura, Atsushi" sort="Okumura, Atsushi" uniqKey="Okumura A" first="Atsushi" last="Okumura">Atsushi Okumura</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Lovaglio, Jamie" sort="Lovaglio, Jamie" uniqKey="Lovaglio J" first="Jamie" last="Lovaglio">Jamie Lovaglio</name>
<affiliation><nlm:affiliation>Rocky Mountain Veterinary Branch and.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Saturday, Greg" sort="Saturday, Greg" uniqKey="Saturday G" first="Greg" last="Saturday">Greg Saturday</name>
<affiliation><nlm:affiliation>Rocky Mountain Veterinary Branch and.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hanley, Patrick W" sort="Hanley, Patrick W" uniqKey="Hanley P" first="Patrick W" last="Hanley">Patrick W. Hanley</name>
<affiliation><nlm:affiliation>Rocky Mountain Veterinary Branch and.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Meade White, Kimberly" sort="Meade White, Kimberly" uniqKey="Meade White K" first="Kimberly" last="Meade-White">Kimberly Meade-White</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Williamson, Brandi N" sort="Williamson, Brandi N" uniqKey="Williamson B" first="Brandi N" last="Williamson">Brandi N. Williamson</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hansen, Frederick" sort="Hansen, Frederick" uniqKey="Hansen F" first="Frederick" last="Hansen">Frederick Hansen</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Perez Perez, Lizette" sort="Perez Perez, Lizette" uniqKey="Perez Perez L" first="Lizette" last="Perez-Perez">Lizette Perez-Perez</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Leventhal, Shanna" sort="Leventhal, Shanna" uniqKey="Leventhal S" first="Shanna" last="Leventhal">Shanna Leventhal</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Tang Huau, Tsing Lee" sort="Tang Huau, Tsing Lee" uniqKey="Tang Huau T" first="Tsing-Lee" last="Tang-Huau">Tsing-Lee Tang-Huau</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Callison, Julie" sort="Callison, Julie" uniqKey="Callison J" first="Julie" last="Callison">Julie Callison</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Stromberg, Kaitlin A" sort="Stromberg, Kaitlin A" uniqKey="Stromberg K" first="Kaitlin A" last="Stromberg">Kaitlin A. Stromberg</name>
<affiliation><nlm:affiliation>Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name>
<affiliation><nlm:affiliation>Rocky Mountain Veterinary Branch and.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Sewell, Graham" sort="Sewell, Graham" uniqKey="Sewell G" first="Graham" last="Sewell">Graham Sewell</name>
<affiliation><nlm:affiliation>Leicester School of Pharmacy, De Montfort University, Leicester, United Kingdom.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Bosio, Catharine M" sort="Bosio, Catharine M" uniqKey="Bosio C" first="Catharine M" last="Bosio">Catharine M. Bosio</name>
<affiliation><nlm:affiliation>Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hawman, David" sort="Hawman, David" uniqKey="Hawman D" first="David" last="Hawman">David Hawman</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name>
<affiliation><nlm:affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</nlm:affiliation>
</affiliation>
</author>
</analytic>
<series><title level="j">JCI insight</title>
<idno type="eISSN">2379-3708</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Antiviral Agents (therapeutic use)</term>
<term>COVID-19 (drug therapy)</term>
<term>COVID-19 (pathology)</term>
<term>COVID-19 (prevention & control)</term>
<term>COVID-19 (therapy)</term>
<term>Chlorocebus aethiops (MeSH)</term>
<term>Cricetinae (MeSH)</term>
<term>Cytokines (metabolism)</term>
<term>Disease Models, Animal (MeSH)</term>
<term>Drug Evaluation, Preclinical (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Hydroxychloroquine (therapeutic use)</term>
<term>Lung (pathology)</term>
<term>Lung (virology)</term>
<term>Macaca mulatta (MeSH)</term>
<term>Male (MeSH)</term>
<term>SARS-CoV-2 (drug effects)</term>
<term>Treatment Outcome (MeSH)</term>
<term>Vero Cells (MeSH)</term>
<term>Viral Load (drug effects)</term>
<term>Virus Replication (drug effects)</term>
<term>Virus Shedding (drug effects)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Antiviral Agents</term>
<term>Hydroxychloroquine</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS-CoV-2</term>
<term>Viral Load</term>
<term>Virus Replication</term>
<term>Virus Shedding</term>
</keywords>
<keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>COVID-19</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>COVID-19</term>
<term>Lung</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>COVID-19</term>
</keywords>
<keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>COVID-19</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Cricetinae</term>
<term>Disease Models, Animal</term>
<term>Drug Evaluation, Preclinical</term>
<term>Humans</term>
<term>Macaca mulatta</term>
<term>Male</term>
<term>Treatment Outcome</term>
<term>Vero Cells</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We remain largely without effective prophylactic/therapeutic interventions for COVID-19. Although many human COVID-19 clinical trials are ongoing, there remains a deficiency of supportive preclinical drug efficacy studies to help guide decisions. Here we assessed the prophylactic/therapeutic efficacy of hydroxychloroquine (HCQ), a drug of interest for COVID-19 management, in 2 animal disease models. The standard human malaria HCQ prophylaxis (6.5 mg/kg given weekly) and treatment (6.5 mg/kg given daily) did not significantly benefit clinical outcome, nor did it reduce SARS-CoV-2 replication/shedding in the upper and lower respiratory tract in the rhesus macaque disease model. Similarly, when used for prophylaxis or treatment, neither the standard human malaria dose (6.5 mg/kg) nor a high dose (50 mg/kg) of HCQ had any beneficial effect on clinical disease or SARS-CoV-2 kinetics (replication/shedding) in the Syrian hamster disease model. Results from these 2 preclinical animal models may prove helpful in guiding clinical use of HCQ for prophylaxis/treatment of COVID-19.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">33090972</PMID>
<DateCompleted><Year>2020</Year>
<Month>12</Month>
<Day>16</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>12</Month>
<Day>16</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2379-3708</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>5</Volume>
<Issue>23</Issue>
<PubDate><Year>2020</Year>
<Month>12</Month>
<Day>03</Day>
</PubDate>
</JournalIssue>
<Title>JCI insight</Title>
<ISOAbbreviation>JCI Insight</ISOAbbreviation>
</Journal>
<ArticleTitle>Hydroxychloroquine prophylaxis and treatment is ineffective in macaque and hamster SARS-CoV-2 disease models.</ArticleTitle>
<ELocationID EIdType="doi" ValidYN="Y">10.1172/jci.insight.143174</ELocationID>
<ELocationID EIdType="pii" ValidYN="Y">143174</ELocationID>
<Abstract><AbstractText>We remain largely without effective prophylactic/therapeutic interventions for COVID-19. Although many human COVID-19 clinical trials are ongoing, there remains a deficiency of supportive preclinical drug efficacy studies to help guide decisions. Here we assessed the prophylactic/therapeutic efficacy of hydroxychloroquine (HCQ), a drug of interest for COVID-19 management, in 2 animal disease models. The standard human malaria HCQ prophylaxis (6.5 mg/kg given weekly) and treatment (6.5 mg/kg given daily) did not significantly benefit clinical outcome, nor did it reduce SARS-CoV-2 replication/shedding in the upper and lower respiratory tract in the rhesus macaque disease model. Similarly, when used for prophylaxis or treatment, neither the standard human malaria dose (6.5 mg/kg) nor a high dose (50 mg/kg) of HCQ had any beneficial effect on clinical disease or SARS-CoV-2 kinetics (replication/shedding) in the Syrian hamster disease model. Results from these 2 preclinical animal models may prove helpful in guiding clinical use of HCQ for prophylaxis/treatment of COVID-19.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rosenke</LastName>
<ForeName>Kyle</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jarvis</LastName>
<ForeName>Michael A</ForeName>
<Initials>MA</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>University of Plymouth, Plymouth, Devon, United Kingdom; The Vaccine Group Ltd, Plymouth, Devon, United Kingdom.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Feldmann</LastName>
<ForeName>Friederike</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch and.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Schwarz</LastName>
<ForeName>Benjamin</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Okumura</LastName>
<ForeName>Atsushi</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lovaglio</LastName>
<ForeName>Jamie</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch and.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Saturday</LastName>
<ForeName>Greg</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch and.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hanley</LastName>
<ForeName>Patrick W</ForeName>
<Initials>PW</Initials>
<AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch and.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Meade-White</LastName>
<ForeName>Kimberly</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Williamson</LastName>
<ForeName>Brandi N</ForeName>
<Initials>BN</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hansen</LastName>
<ForeName>Frederick</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Perez-Perez</LastName>
<ForeName>Lizette</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Leventhal</LastName>
<ForeName>Shanna</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tang-Huau</LastName>
<ForeName>Tsing-Lee</ForeName>
<Initials>TL</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Callison</LastName>
<ForeName>Julie</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Haddock</LastName>
<ForeName>Elaine</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Stromberg</LastName>
<ForeName>Kaitlin A</ForeName>
<Initials>KA</Initials>
<AffiliationInfo><Affiliation>Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Scott</LastName>
<ForeName>Dana</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch and.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sewell</LastName>
<ForeName>Graham</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Leicester School of Pharmacy, De Montfort University, Leicester, United Kingdom.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bosio</LastName>
<ForeName>Catharine M</ForeName>
<Initials>CM</Initials>
<AffiliationInfo><Affiliation>Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hawman</LastName>
<ForeName>David</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>de Wit</LastName>
<ForeName>Emmie</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Feldmann</LastName>
<ForeName>Heinz</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052060">Research Support, N.I.H., Intramural</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>12</Month>
<Day>03</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>JCI Insight</MedlineTA>
<NlmUniqueID>101676073</NlmUniqueID>
<ISSNLinking>2379-3708</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D016207">Cytokines</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>4QWG6N8QKH</RegistryNumber>
<NameOfSubstance UI="D006886">Hydroxychloroquine</NameOfSubstance>
</Chemical>
</ChemicalList>
<SupplMeshList><SupplMeshName Type="Protocol" UI="C000705127">COVID-19 drug treatment</SupplMeshName>
</SupplMeshList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000998" MajorTopicYN="N">Antiviral Agents</DescriptorName>
<QualifierName UI="Q000627" MajorTopicYN="Y">therapeutic use</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000086382" MajorTopicYN="N">COVID-19</DescriptorName>
<QualifierName UI="Q000188" MajorTopicYN="N">drug therapy</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName>
<QualifierName UI="Q000628" MajorTopicYN="Y">therapy</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004195" MajorTopicYN="N">Disease Models, Animal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004353" MajorTopicYN="N">Drug Evaluation, Preclinical</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006886" MajorTopicYN="N">Hydroxychloroquine</DescriptorName>
<QualifierName UI="Q000627" MajorTopicYN="Y">therapeutic use</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008253" MajorTopicYN="N">Macaca mulatta</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000086402" MajorTopicYN="N">SARS-CoV-2</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016896" MajorTopicYN="N">Treatment Outcome</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019562" MajorTopicYN="N">Viral Load</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017201" MajorTopicYN="N">Virus Shedding</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">COVID-19</Keyword>
<Keyword MajorTopicYN="Y">Cytokines</Keyword>
<Keyword MajorTopicYN="Y">Drug screens</Keyword>
<Keyword MajorTopicYN="Y">Molecular biology</Keyword>
<Keyword MajorTopicYN="Y">Therapeutics</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year>
<Month>08</Month>
<Day>10</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2020</Year>
<Month>10</Month>
<Day>16</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2020</Year>
<Month>10</Month>
<Day>23</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>12</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2020</Year>
<Month>10</Month>
<Day>22</Day>
<Hour>17</Hour>
<Minute>32</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">33090972</ArticleId>
<ArticleId IdType="pii">143174</ArticleId>
<ArticleId IdType="doi">10.1172/jci.insight.143174</ArticleId>
<ArticleId IdType="pmc">PMC7714406</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Crit Care. 1999;3(2):R19-R24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11094478</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2019 Sep 10;9(1):12937</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31506515</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2020 Jan;25(3):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31992387</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Res. 2020 Mar;30(3):269-271</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32020029</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Infect Dis. 2020 Jul 28;71(15):732-739</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32150618</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Br J Clin Pharmacol. 1988 Sep;26(3):303-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3179169</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Discov. 2020 Mar 18;6:16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32194981</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Expert Rev Anti Infect Ther. 2017 May;15(5):483-492</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28286997</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Comp Med. 2010 Oct;60(5):389-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21262125</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMJ. 2020 Apr 1;369:m1335</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32238355</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antimicrob Agents Chemother. 2009 Apr;53(4):1468-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19188392</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Antimicrob Agents. 2020 Jul;56(1):105949</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32205204</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMJ. 2020 May 14;369:m1849</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32409561</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2020 Feb 22;395(10224):565-574</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32007145</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Rheumatol Ther. 2015 Dec;2(2):183-195</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27747530</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Med. 1983 Jul 18;75(1A):11-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6408923</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur Respir J. 1999 Jul;14(1):160-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10489845</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Infect Dis. 2020 Dec 3;71(9):2428-2446</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32215622</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2020 Aug 6;383(6):517-525</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32492293</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2020 Jun 10;12(6):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32532094</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Rheumatol. 2020 Mar;16(3):155-166</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32034323</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virol J. 2005 Aug 22;2:69</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16115318</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Ther Drug Monit. 2016 Apr;38(2):259-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26587870</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Immunopathol. 2016 Jul;38(4):517-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27001429</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 1;1072:320-327</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29207305</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2015 Jun;21(6):1065-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25988934</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Br J Clin Pharmacol. 1989 Jun;27(6):771-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2757893</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2020 Jun 18;382(25):2411-2418</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32379955</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2020 Sep;585(7826):584-587</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32698191</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2020 Feb 15;395(10223):507-513</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32007143</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2020 Sep;585(7824):268-272</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32396922</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/CovidChloroV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000964 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000964 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= CovidChloroV1 |flux= Main |étape= Corpus |type= RBID |clé= pubmed:33090972 |texte= Hydroxychloroquine prophylaxis and treatment is ineffective in macaque and hamster SARS-CoV-2 disease models. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:33090972" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a CovidChloroV1
![]() | This area was generated with Dilib version V0.6.38. | ![]() |