CovidChloroV1, Main, Corpus, bibRecord, 000323

Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor.

Identifieur interne : 000323 ( Main/Corpus ); précédent : 000322; suivant : 000324

Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor.

Auteurs : Jung-Soo Suh ; Heon-Su Kim ; Tae-Jin Kim

Source :

Sensors and actuators. B, Chemical [ 0925-4005 ] ; 2021.

RBID : pubmed:33612970

Abstract

The global outbreak of coronavirus disease and rapid spread of the causative severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represent a significant threat to human health. A key mechanism of human SARS-CoV-2 infection is initiated by the combination of human angiotensin-converting enzyme 2 (hACE2) and the receptor-binding domain (RBD) of the SARS-CoV-2-derived spike glycoprotein. Despite the importance of these protein interactions, there are still insufficient detection methods to observe their activity at the cellular level. Herein, we developed a novel fluorescence resonance energy transfer (FRET)-based hACE2 biosensor to monitor the interaction between hACE2 and SARS-CoV-2 RBD. This biosensor facilitated the visualization of hACE2-RBD activity with high spatiotemporal resolutions at the single-cell level. Further studies revealed that the FRET-based hACE2 biosensors were sensitive to both exogenous and endogenous hACE2 expression, suggesting that they might be safely applied to the early stage of SARS-CoV-2 infection without direct virus use. Therefore, our novel biosensor could potentially help develop drugs that target SARS-CoV-2 by inhibiting hACE2-RBD interaction.

DOI: 10.1016/j.snb.2021.129663
PubMed: 33612970
PubMed Central: PMC7885701

Links to Exploration step

pubmed:33612970

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor.</title>
<author><name sortKey="Suh, Jung Soo" sort="Suh, Jung Soo" uniqKey="Suh J" first="Jung-Soo" last="Suh">Jung-Soo Suh</name>
<affiliation><nlm:affiliation>Department of Integrated Biological Science, Pusan National University, Pusan 46241, Republic of Korea.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Kim, Heon Su" sort="Kim, Heon Su" uniqKey="Kim H" first="Heon-Su" last="Kim">Heon-Su Kim</name>
<affiliation><nlm:affiliation>Department of Integrated Biological Science, Pusan National University, Pusan 46241, Republic of Korea.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Kim, Tae Jin" sort="Kim, Tae Jin" uniqKey="Kim T" first="Tae-Jin" last="Kim">Tae-Jin Kim</name>
<affiliation><nlm:affiliation>Department of Integrated Biological Science, Pusan National University, Pusan 46241, Republic of Korea.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>Department of Biological Sciences, Pusan National University, Pusan 46241, Republic of Korea.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>Institute of Systems Biology, Pusan National University, Pusan 46241, Republic of Korea.</nlm:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2021">2021</date>
<idno type="RBID">pubmed:33612970</idno>
<idno type="pmid">33612970</idno>
<idno type="doi">10.1016/j.snb.2021.129663</idno>
<idno type="pmc">PMC7885701</idno>
<idno type="wicri:Area/Main/Corpus">000323</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000323</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor.</title>
<author><name sortKey="Suh, Jung Soo" sort="Suh, Jung Soo" uniqKey="Suh J" first="Jung-Soo" last="Suh">Jung-Soo Suh</name>
<affiliation><nlm:affiliation>Department of Integrated Biological Science, Pusan National University, Pusan 46241, Republic of Korea.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Kim, Heon Su" sort="Kim, Heon Su" uniqKey="Kim H" first="Heon-Su" last="Kim">Heon-Su Kim</name>
<affiliation><nlm:affiliation>Department of Integrated Biological Science, Pusan National University, Pusan 46241, Republic of Korea.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Kim, Tae Jin" sort="Kim, Tae Jin" uniqKey="Kim T" first="Tae-Jin" last="Kim">Tae-Jin Kim</name>
<affiliation><nlm:affiliation>Department of Integrated Biological Science, Pusan National University, Pusan 46241, Republic of Korea.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>Department of Biological Sciences, Pusan National University, Pusan 46241, Republic of Korea.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>Institute of Systems Biology, Pusan National University, Pusan 46241, Republic of Korea.</nlm:affiliation>
</affiliation>
</author>
</analytic>
<series><title level="j">Sensors and actuators. B, Chemical</title>
<idno type="ISSN">0925-4005</idno>
<imprint><date when="2021" type="published">2021</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The global outbreak of coronavirus disease and rapid spread of the causative severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represent a significant threat to human health. A key mechanism of human SARS-CoV-2 infection is initiated by the combination of human angiotensin-converting enzyme 2 (hACE2) and the receptor-binding domain (RBD) of the SARS-CoV-2-derived spike glycoprotein. Despite the importance of these protein interactions, there are still insufficient detection methods to observe their activity at the cellular level. Herein, we developed a novel fluorescence resonance energy transfer (FRET)-based hACE2 biosensor to monitor the interaction between hACE2 and SARS-CoV-2 RBD. This biosensor facilitated the visualization of hACE2-RBD activity with high spatiotemporal resolutions at the single-cell level. Further studies revealed that the FRET-based hACE2 biosensors were sensitive to both exogenous and endogenous hACE2 expression, suggesting that they might be safely applied to the early stage of SARS-CoV-2 infection without direct virus use. Therefore, our novel biosensor could potentially help develop drugs that target SARS-CoV-2 by inhibiting hACE2-RBD interaction.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33612970</PMID>
<DateRevised><Year>2021</Year>
<Month>03</Month>
<Day>12</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0925-4005</ISSN>
<JournalIssue CitedMedium="Print"><Volume>334</Volume>
<PubDate><Year>2021</Year>
<Month>May</Month>
<Day>01</Day>
</PubDate>
</JournalIssue>
<Title>Sensors and actuators. B, Chemical</Title>
<ISOAbbreviation>Sens Actuators B Chem</ISOAbbreviation>
</Journal>
<ArticleTitle>Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor.</ArticleTitle>
<Pagination><MedlinePgn>129663</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.snb.2021.129663</ELocationID>
<Abstract><AbstractText>The global outbreak of coronavirus disease and rapid spread of the causative severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represent a significant threat to human health. A key mechanism of human SARS-CoV-2 infection is initiated by the combination of human angiotensin-converting enzyme 2 (hACE2) and the receptor-binding domain (RBD) of the SARS-CoV-2-derived spike glycoprotein. Despite the importance of these protein interactions, there are still insufficient detection methods to observe their activity at the cellular level. Herein, we developed a novel fluorescence resonance energy transfer (FRET)-based hACE2 biosensor to monitor the interaction between hACE2 and SARS-CoV-2 RBD. This biosensor facilitated the visualization of hACE2-RBD activity with high spatiotemporal resolutions at the single-cell level. Further studies revealed that the FRET-based hACE2 biosensors were sensitive to both exogenous and endogenous hACE2 expression, suggesting that they might be safely applied to the early stage of SARS-CoV-2 infection without direct virus use. Therefore, our novel biosensor could potentially help develop drugs that target SARS-CoV-2 by inhibiting hACE2-RBD interaction.</AbstractText>
<CopyrightInformation>© 2021 Elsevier B.V. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Suh</LastName>
<ForeName>Jung-Soo</ForeName>
<Initials>JS</Initials>
<AffiliationInfo><Affiliation>Department of Integrated Biological Science, Pusan National University, Pusan 46241, Republic of Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Heon-Su</ForeName>
<Initials>HS</Initials>
<AffiliationInfo><Affiliation>Department of Integrated Biological Science, Pusan National University, Pusan 46241, Republic of Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Tae-Jin</ForeName>
<Initials>TJ</Initials>
<AffiliationInfo><Affiliation>Department of Integrated Biological Science, Pusan National University, Pusan 46241, Republic of Korea.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Biological Sciences, Pusan National University, Pusan 46241, Republic of Korea.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute of Systems Biology, Pusan National University, Pusan 46241, Republic of Korea.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2021</Year>
<Month>02</Month>
<Day>16</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Switzerland</Country>
<MedlineTA>Sens Actuators B Chem</MedlineTA>
<NlmUniqueID>101149755</NlmUniqueID>
<ISSNLinking>0925-4005</ISSNLinking>
</MedlineJournalInfo>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ACE2</Keyword>
<Keyword MajorTopicYN="N">Biosensor</Keyword>
<Keyword MajorTopicYN="N">CQ, chloroquine</Keyword>
<Keyword MajorTopicYN="N">FRET</Keyword>
<Keyword MajorTopicYN="N">HCQ, hydroxychloroquine</Keyword>
<Keyword MajorTopicYN="N">Live-cell imaging</Keyword>
<Keyword MajorTopicYN="N">NA, numerical aperture</Keyword>
<Keyword MajorTopicYN="N">RBD, receptor-binding domain</Keyword>
<Keyword MajorTopicYN="N">RBM, receptor-binding motif</Keyword>
<Keyword MajorTopicYN="N">ROI, region of interest</Keyword>
<Keyword MajorTopicYN="N">SARS-CoV-2</Keyword>
<Keyword MajorTopicYN="N">SARS-CoV-2, severe acute respiratory syndrome coronavirus 2</Keyword>
<Keyword MajorTopicYN="N">SEM, standard error of the mean</Keyword>
<Keyword MajorTopicYN="N">bg, background</Keyword>
<Keyword MajorTopicYN="N">hACE2, human angiotensin-converting enzyme 2</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year>
<Month>10</Month>
<Day>05</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2020</Year>
<Month>12</Month>
<Day>16</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2021</Year>
<Month>02</Month>
<Day>10</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2021</Year>
<Month>2</Month>
<Day>23</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2021</Year>
<Month>2</Month>
<Day>23</Day>
<Hour>6</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2021</Year>
<Month>2</Month>
<Day>22</Day>
<Hour>5</Hour>
<Minute>51</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">33612970</ArticleId>
<ArticleId IdType="doi">10.1016/j.snb.2021.129663</ArticleId>
<ArticleId IdType="pii">S0925-4005(21)00231-8</ArticleId>
<ArticleId IdType="pmc">PMC7885701</ArticleId>
</ArticleIdList>
<pmc-dir>pmcsd</pmc-dir>
        <ReferenceList><Reference><Citation>J Neurochem. 2008 Dec;107(6):1482-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19014390</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Pharmacol. 2011 Apr;11(2):150-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21215698</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Discov. 2020 Mar 18;6:16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32194981</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2002 Jun 20;417(6891):822-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12075344</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2020 Mar 17;94(7):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31996437</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pathol. 2004 Jun;203(2):631-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15141377</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Circ Res. 2000 Sep 1;87(5):E1-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10969042</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virol J. 2005 Aug 22;2:69</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16115318</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2020 Mar 27;367(6485):1444-1448</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32132184</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Cardiol. 2014 Jul;11(7):413-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24776703</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pathol. 2007 May;212(1):1-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17464936</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Pharmacol. 2020 Nov 20;11:574720</Citation>
<ArticleIdList><ArticleId IdType="pubmed">33658924</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Physiol Lung Cell Mol Physiol. 2009 Jul;297(1):L84-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19411314</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2020 Mar;579(7798):265-269</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32015508</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Hepatol. 2007 Sep;47(3):387-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17532087</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Pharmacol Res. 2016 May;107:154-162</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26995300</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Nucl Med Mol Imaging. 2020 May;47(5):1275-1280</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32107577</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cardiovasc Res. 2020 May 1;116(6):1097-1100</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32227090</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2020 Mar 26;382(13):1199-1207</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31995857</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hypertension. 2006 Mar;47(3):515-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16365192</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virol Sin. 2020 Jun;35(3):266-271</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32125642</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 1951 Oct 27;2(6687):755-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14874500</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Mol Immunol. 2020 Jun;17(6):621-630</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32415260</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Neuroimmune Pharmacol. 2020 Jun;15(2):174-180</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32415419</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Molecules. 2020 Sep 10;25(18):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32927621</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Heart Fail Rep. 2014 Mar;11(1):58-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24293035</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2020 Sep 11;11(1):4541</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32917884</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Rev Allergy Immunol. 2012 Apr;42(2):145-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21221847</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2020 May;581(7807):221-224</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32225175</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Physiol Heart Circ Physiol. 2005 Dec;289(6):H2281-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16055515</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Ann Intern Med. 1993 Dec 1;119(11):1067-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8239224</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eye (Lond). 2020 Jul;34(7):1212-1219</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32382146</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>DNA Seq. 2002 Aug;13(4):217-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12487024</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Jan 30;314(1):235-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14715271</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phytomedicine. 2020 Dec;79:153333</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32920291</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2020 Jun 30;527(3):702-708</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32410735</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/CovidChloroV1/Data/Main/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000323 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000323 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    CovidChloroV1
   |flux=    Main
   |étape=   Corpus
   |type=    RBID
   |clé=     pubmed:33612970
   |texte=   Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i   -Sk "pubmed:33612970" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd   \
       | NlmPubMed2Wicri -a CovidChloroV1

This area was generated with Dilib version V0.6.38.
Data generation: Sat May 22 17:02:32 2021. Site generation: Sat May 22 17:06:52 2021

	Serveur d'exploration COVID et hydrochloroquine
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration COVID et hydrochloroquine

Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor.

Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor.

Source :

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki