Immunomodulatory proteins FIP-gts and chloroquine induce caspase-independent cell death via autophagy for resensitizing cisplatin-resistant urothelial cancer cells.
Identifieur interne : 000189 ( PubMed/Corpus ); précédent : 000188; suivant : 000190Immunomodulatory proteins FIP-gts and chloroquine induce caspase-independent cell death via autophagy for resensitizing cisplatin-resistant urothelial cancer cells.
Auteurs : I-Lun Hsin ; Shao-Chuan Wang ; Jian-Ri Li ; Tsai-Chun Ciou ; Chih-Hsien Wu ; Hung-Ming Wu ; Jiunn-Liang KoSource :
- Phytomedicine : international journal of phytotherapy and phytopharmacology [ 1618-095X ] ; 2016.
English descriptors
- KwdEn :
- Amino Acid Chloromethyl Ketones (pharmacology), Antineoplastic Combined Chemotherapy Protocols (pharmacology), Apoptosis (drug effects), Autophagy (drug effects), Caspases (metabolism), Cell Cycle (drug effects), Cell Line, Tumor, Cell Survival (drug effects), Chloroquine (administration & dosage), Chloroquine (pharmacology), Cisplatin (pharmacology), Drug Resistance, Neoplasm (drug effects), Fungal Proteins (administration & dosage), Fungal Proteins (pharmacology), Ganoderma (chemistry), Humans, Immunologic Factors (pharmacology), Membrane Potential, Mitochondrial (drug effects), Urologic Neoplasms (drug therapy), Urologic Neoplasms (pathology).
- MESH :
- chemical , administration & dosage : Chloroquine, Fungal Proteins.
- chemical , metabolism : Caspases.
- chemical , pharmacology : Amino Acid Chloromethyl Ketones, Chloroquine, Cisplatin, Fungal Proteins, Immunologic Factors.
- chemistry : Ganoderma.
- drug effects : Apoptosis, Autophagy, Cell Cycle, Cell Survival, Drug Resistance, Neoplasm, Membrane Potential, Mitochondrial.
- drug therapy : Urologic Neoplasms.
- pathology : Urologic Neoplasms.
- pharmacology : Antineoplastic Combined Chemotherapy Protocols.
- Cell Line, Tumor, Humans.
Abstract
Chloroquine, a lysosomal inhibitor, is used for malaria, rheumatoid arthritis, and lupus erythematosus therapy. In our previous study, FIP-gts, an immunomodulatory protein from Ganoderma tsugae, inhibited cell viability in lung cancer cells and urothelial cancer cells. Urothelial carcinoma is the most common type of bladder cancer. Cisplatin resistance is an important issue in urothelial carcinoma therapy.
DOI: 10.1016/j.phymed.2016.09.003
PubMed: 27823620
Links to Exploration step
pubmed:27823620Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Immunomodulatory proteins FIP-gts and chloroquine induce caspase-independent cell death via autophagy for resensitizing cisplatin-resistant urothelial cancer cells.</title><author><name sortKey="Hsin, I Lun" sort="Hsin, I Lun" uniqKey="Hsin I" first="I-Lun" last="Hsin">I-Lun Hsin</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Inflammation Research & Drug Development Center, Changhua Christian Hospital, Changhua, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Shao Chuan" sort="Wang, Shao Chuan" uniqKey="Wang S" first="Shao-Chuan" last="Wang">Shao-Chuan Wang</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Urology, Chung-Shan Medical University Hospital, Taichung, Taiwan; School of Medicine, Chung-Shan Medical University, Taichung, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Jian Ri" sort="Li, Jian Ri" uniqKey="Li J" first="Jian-Ri" last="Li">Jian-Ri Li</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Urology, Department of Surgery, Taichung Veterans General Hospital, Taichung, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Ciou, Tsai Chun" sort="Ciou, Tsai Chun" uniqKey="Ciou T" first="Tsai-Chun" last="Ciou">Tsai-Chun Ciou</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Chih Hsien" sort="Wu, Chih Hsien" uniqKey="Wu C" first="Chih-Hsien" last="Wu">Chih-Hsien Wu</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Hung Ming" sort="Wu, Hung Ming" uniqKey="Wu H" first="Hung-Ming" last="Wu">Hung-Ming Wu</name><affiliation><nlm:affiliation>Inflammation Research & Drug Development Center, Changhua Christian Hospital, Changhua, Taiwan; Graduate Institute of Acupuncture Science, China Medical University, Taichung, Taiwan; Department of Neurology, Changhua Christian Hospital, Changhua, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Ko, Jiunn Liang" sort="Ko, Jiunn Liang" uniqKey="Ko J" first="Jiunn-Liang" last="Ko">Jiunn-Liang Ko</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Urology, Chung-Shan Medical University Hospital, Taichung, Taiwan; School of Medicine, Chung-Shan Medical University, Taichung, Taiwan. Electronic address: jlko1007@gmail.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27823620</idno><idno type="pmid">27823620</idno><idno type="doi">10.1016/j.phymed.2016.09.003</idno><idno type="wicri:Area/PubMed/Corpus">000189</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000189</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Immunomodulatory proteins FIP-gts and chloroquine induce caspase-independent cell death via autophagy for resensitizing cisplatin-resistant urothelial cancer cells.</title><author><name sortKey="Hsin, I Lun" sort="Hsin, I Lun" uniqKey="Hsin I" first="I-Lun" last="Hsin">I-Lun Hsin</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Inflammation Research & Drug Development Center, Changhua Christian Hospital, Changhua, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Shao Chuan" sort="Wang, Shao Chuan" uniqKey="Wang S" first="Shao-Chuan" last="Wang">Shao-Chuan Wang</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Urology, Chung-Shan Medical University Hospital, Taichung, Taiwan; School of Medicine, Chung-Shan Medical University, Taichung, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Jian Ri" sort="Li, Jian Ri" uniqKey="Li J" first="Jian-Ri" last="Li">Jian-Ri Li</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Urology, Department of Surgery, Taichung Veterans General Hospital, Taichung, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Ciou, Tsai Chun" sort="Ciou, Tsai Chun" uniqKey="Ciou T" first="Tsai-Chun" last="Ciou">Tsai-Chun Ciou</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Chih Hsien" sort="Wu, Chih Hsien" uniqKey="Wu C" first="Chih-Hsien" last="Wu">Chih-Hsien Wu</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Hung Ming" sort="Wu, Hung Ming" uniqKey="Wu H" first="Hung-Ming" last="Wu">Hung-Ming Wu</name><affiliation><nlm:affiliation>Inflammation Research & Drug Development Center, Changhua Christian Hospital, Changhua, Taiwan; Graduate Institute of Acupuncture Science, China Medical University, Taichung, Taiwan; Department of Neurology, Changhua Christian Hospital, Changhua, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Ko, Jiunn Liang" sort="Ko, Jiunn Liang" uniqKey="Ko J" first="Jiunn-Liang" last="Ko">Jiunn-Liang Ko</name><affiliation><nlm:affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Urology, Chung-Shan Medical University Hospital, Taichung, Taiwan; School of Medicine, Chung-Shan Medical University, Taichung, Taiwan. Electronic address: jlko1007@gmail.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Phytomedicine : international journal of phytotherapy and phytopharmacology</title><idno type="eISSN">1618-095X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Chloromethyl Ketones (pharmacology)</term><term>Antineoplastic Combined Chemotherapy Protocols (pharmacology)</term><term>Apoptosis (drug effects)</term><term>Autophagy (drug effects)</term><term>Caspases (metabolism)</term><term>Cell Cycle (drug effects)</term><term>Cell Line, Tumor</term><term>Cell Survival (drug effects)</term><term>Chloroquine (administration & dosage)</term><term>Chloroquine (pharmacology)</term><term>Cisplatin (pharmacology)</term><term>Drug Resistance, Neoplasm (drug effects)</term><term>Fungal Proteins (administration & dosage)</term><term>Fungal Proteins (pharmacology)</term><term>Ganoderma (chemistry)</term><term>Humans</term><term>Immunologic Factors (pharmacology)</term><term>Membrane Potential, Mitochondrial (drug effects)</term><term>Urologic Neoplasms (drug therapy)</term><term>Urologic Neoplasms (pathology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Chloroquine</term><term>Fungal Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Caspases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Amino Acid Chloromethyl Ketones</term><term>Chloroquine</term><term>Cisplatin</term><term>Fungal Proteins</term><term>Immunologic Factors</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Ganoderma</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term><term>Autophagy</term><term>Cell Cycle</term><term>Cell Survival</term><term>Drug Resistance, Neoplasm</term><term>Membrane Potential, Mitochondrial</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Urologic Neoplasms</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Urologic Neoplasms</term></keywords><keywords scheme="MESH" qualifier="pharmacology" xml:lang="en"><term>Antineoplastic Combined Chemotherapy Protocols</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chloroquine, a lysosomal inhibitor, is used for malaria, rheumatoid arthritis, and lupus erythematosus therapy. In our previous study, FIP-gts, an immunomodulatory protein from Ganoderma tsugae, inhibited cell viability in lung cancer cells and urothelial cancer cells. Urothelial carcinoma is the most common type of bladder cancer. Cisplatin resistance is an important issue in urothelial carcinoma therapy.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">27823620</PMID><DateCompleted><Year>2017</Year><Month>06</Month><Day>05</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-095X</ISSN><JournalIssue CitedMedium="Internet"><Volume>23</Volume><Issue>13</Issue><PubDate><Year>2016</Year><Month>Dec</Month><Day>01</Day></PubDate></JournalIssue><Title>Phytomedicine : international journal of phytotherapy and phytopharmacology</Title><ISOAbbreviation>Phytomedicine</ISOAbbreviation></Journal><ArticleTitle>Immunomodulatory proteins FIP-gts and chloroquine induce caspase-independent cell death via autophagy for resensitizing cisplatin-resistant urothelial cancer cells.</ArticleTitle><Pagination><MedlinePgn>1566-1573</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0944-7113(16)30157-X</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.phymed.2016.09.003</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Chloroquine, a lysosomal inhibitor, is used for malaria, rheumatoid arthritis, and lupus erythematosus therapy. In our previous study, FIP-gts, an immunomodulatory protein from Ganoderma tsugae, inhibited cell viability in lung cancer cells and urothelial cancer cells. Urothelial carcinoma is the most common type of bladder cancer. Cisplatin resistance is an important issue in urothelial carcinoma therapy.</AbstractText><AbstractText Label="PURPOSE" NlmCategory="OBJECTIVE">The aim of this study is to investigate the effect of combination treatment with FIP-gts and chloroquine on cytotoxicity to resensitize the cisplatin-resistant cells.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">FIP-gts and chloroquine cytotoxicity were determined by evaluating CCK-8 assay. Cell death pathways, ROS and cell cycle arrested were analysed through flow cytometry and Western blot. ShRNA targeting to autophagy-related genes were tested to evaluate their autophagic cell death for resistant urothelial cells.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Using CCK-8 assay, chloroquine increased FIP-gts-induced cytotoxicity in parental and cisplatin-resistant urothelial cancer cell lines. On flow cytometry, chloroquine enhanced FIP-gts-mediated sub-G1 accumulation, annexin V positive signal and mitochondrial membrane potential loss. Caspase-3/PARP cascade and z-VAD-fmk were performed to prove that FIP-gts and chloroquine induced caspase-independent cell death. Using H2DCFDA staining and flow cytometry, FIP-gts and chloroquine did not induce ROS production. N-acetyl cysteine, a ROS scavenger, inhibited the cytotoxicity and LC3-II accumulation in FIP-gts and chloroquine-treated N/P cells. To elucidate the role of autophagy in caspase-independent cell death by FIP-gts and chloroquine, LC3 shRNA were used to inhibit autophagy in N/P cells. The capabilities of FIP-gts and chloroquine to induce cytotoxicity and sub-G1 phase accumulation were abolished in autophagy-defective cells. This is the first study to reveal the novel function of FIP-gts in triggering caspase-independent cell death in cisplatin-resistant urothelial cancer cells.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Chloroquine enhanced FIP-gts-induced autophagy dependent caspase-independent cell death via abundant autophagosome accumulation. Combination treatment with FIP-gts and chloroquine may provide a new strategy for urothelial cancer therapy.</AbstractText><CopyrightInformation>Copyright © 2016 Elsevier GmbH. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hsin</LastName><ForeName>I-Lun</ForeName><Initials>IL</Initials><AffiliationInfo><Affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Inflammation Research & Drug Development Center, Changhua Christian Hospital, Changhua, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Shao-Chuan</ForeName><Initials>SC</Initials><AffiliationInfo><Affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Urology, Chung-Shan Medical University Hospital, Taichung, Taiwan; School of Medicine, Chung-Shan Medical University, Taichung, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jian-Ri</ForeName><Initials>JR</Initials><AffiliationInfo><Affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Urology, Department of Surgery, Taichung Veterans General Hospital, Taichung, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ciou</LastName><ForeName>Tsai-Chun</ForeName><Initials>TC</Initials><AffiliationInfo><Affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Chih-Hsien</ForeName><Initials>CH</Initials><AffiliationInfo><Affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Hung-Ming</ForeName><Initials>HM</Initials><AffiliationInfo><Affiliation>Inflammation Research & Drug Development Center, Changhua Christian Hospital, Changhua, Taiwan; Graduate Institute of Acupuncture Science, China Medical University, Taichung, Taiwan; Department of Neurology, Changhua Christian Hospital, Changhua, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ko</LastName><ForeName>Jiunn-Liang</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Urology, Chung-Shan Medical University Hospital, Taichung, Taiwan; School of Medicine, Chung-Shan Medical University, Taichung, Taiwan. Electronic address: jlko1007@gmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>09</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Phytomedicine</MedlineTA><NlmUniqueID>9438794</NlmUniqueID><ISSNLinking>0944-7113</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000590">Amino Acid Chloromethyl Ketones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007155">Immunologic Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C096713">benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone</NameOfSubstance></Chemical><Chemical><RegistryNumber>886U3H6UFF</RegistryNumber><NameOfSubstance UI="D002738">Chloroquine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="D020169">Caspases</NameOfSubstance></Chemical><Chemical><RegistryNumber>Q20Q21Q62J</RegistryNumber><NameOfSubstance UI="D002945">Cisplatin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000590" MajorTopicYN="N">Amino Acid Chloromethyl Ketones</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000971" MajorTopicYN="N">Antineoplastic Combined Chemotherapy Protocols</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="N">Apoptosis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001343" MajorTopicYN="N">Autophagy</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020169" MajorTopicYN="N">Caspases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002453" MajorTopicYN="N">Cell Cycle</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045744" MajorTopicYN="N">Cell Line, Tumor</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002738" MajorTopicYN="N">Chloroquine</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002945" MajorTopicYN="N">Cisplatin</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019008" MajorTopicYN="N">Drug Resistance, Neoplasm</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032902" MajorTopicYN="N">Ganoderma</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007155" MajorTopicYN="N">Immunologic Factors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053078" MajorTopicYN="N">Membrane Potential, Mitochondrial</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014571" MajorTopicYN="N">Urologic Neoplasms</DescriptorName><QualifierName UI="Q000188" MajorTopicYN="Y">drug therapy</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Abundant autophagosome accumulation stress</Keyword><Keyword MajorTopicYN="Y">Autophagy</Keyword><Keyword MajorTopicYN="Y">Caspase-independent cell death</Keyword><Keyword MajorTopicYN="Y">Chloroquine</Keyword><Keyword MajorTopicYN="Y">Cisplatin-resistant urothelial cells</Keyword><Keyword MajorTopicYN="Y">FIP-gts</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>05</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>08</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>09</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>11</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>11</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>6</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27823620</ArticleId><ArticleId IdType="pii">S0944-7113(16)30157-X</ArticleId><ArticleId IdType="doi">10.1016/j.phymed.2016.09.003</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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